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Cloning and characterization of 66 kDa streptavidin-binding peptides(SBP)of Pisum sativum *** specific to ***

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BIOCELL 2019年第3期43卷 155-166页

作者： MAHMOUD MOUSTAFA SAAD ALAMRI tarek taha ali SHATI SULAIMAN ALRUMMAN MOHAMED ALKAHTANI Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide(SBP)found in the germinated embryos of Pisum sativum *** pea(*** ***)embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos,but not in the *** total RNA was isolated from embryos of *** but could not be isolated from the *** partial nucleotides sequences of 66 kDa SBPs of embryonic stalk(*** ***)were cloned and identified using pMOSBlue vector.66 kDa(SBP)gene from the embryos of *** *** possesses 327 bp having an open reading frame(ORF)region in a part of the gene that encoded for 108 amino *** showed similarity among 66 kDa SBPs *** ***,with *** seed biotinylated protein(SBP65)and *** sbp65a mRNA with DNA distance matrix between 0.0094 to ***-TOF mass spectrometry analysis of 66 kDa(SBP)proteins showed it had similar short peptides to 19 proteins found in different organisms,especially Convicilin precursor,and the seed biotinylated protein in *** alignment results of both nucleotide sequences and amino acid residues either from cloning or MALDI-TOF-MS showed differences with related species,especially *** mRNA was found in the cotyledons during seeds germination,which means no metabolic activities and this part may act only as food reservoirs for growing newly embryos.

关键词： Cloning Embryo 66 kDa Pisum sativum Streptavidin-binding peptides(SBP)

Analysis of BrainMRI: AI-Assisted Healthcare Framework for the Smart Cities

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Intelligent Automation & Soft Computing 2023年第2期35卷 1843-1856页

作者： Walid El-Shafai Randa ali Ahmed Sedik taha El-sayed taha Mohammed Abd-Elnaby Fathi E.Abd El-Samie Department Electronics and Electrical Communications Engineering Faculty of Electronic EngineeringMenoufia UniversityMenoufia32952Egypt Department of ROBOTICS and Intelligent Machines Faculty of Artificial IntelligenceKafrelsheikh UniversityKafrelsheikhEgypt Department of Computer Engineering College of Computers and Information TechnologyTaif UniversityTaif21944Saudi Arabia

The use of intelligent machines to work and react like humans is vital in emerging smart ***-aided analysis of complex and huge MRI(Mag-netic Resonance Imaging)scans is very important in healthcare *** AI(Artiﬁcial Intelligence)driven healthcare applications,tumor detection is one of the contemporary researchﬁelds that have become attractive to *** are several modalities of imaging performed on the brain for the pur-pose of tumor *** paper offers a deep learning approach for detecting brain tumors from MR(Magnetic Resonance)images based on changes in the division of the training and testing data and the structure of the CNN(Convolu-tional Neural Network)*** proposed approach is carried out on a brain tumor dataset from the National Centre of Image-Guided Therapy,including about 4700 MRI images of ten brain tumor cases with both normal and abnormal *** dataset is divided into test,and train subsets with a ratio of the training set to the validation set of 70:*** main contribution of this paper is introdu-cing an optimum deep learning structure of CNN *** simulation results are obtained for 50 epochs in the training *** simulation results reveal that the optimum CNN architecture consists of four layers.

关键词： Healthcare smart cities clinical automation CNN machine learning brain tumor medical diagnosis

Gene expression of 49 kDa apyrase,cytoskeletal proteins,ATPase,ADPase and amino acid contents of Pisum sativum(L.)cells germinated in Euryops arabicus(*** Jaub.&Spach)water extract

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BIOCELL 2019年第2期43卷 81-87页

作者： MAHMOUD MOUSTAFA SAAD ALAMRI HOIDA ZAKI NAGLAA LOUTFY tarek taha ali SHATI MOHAMED ALKAHTANI SAJDA SIDDIQI Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases,cytoskeletal proteins,ATPases,ADPase and amount of amino acid of pea(Pisum sativum ***).Pellets of cytoskeletals proteins(27000 xg)were probed with anti-apyrase antibody,biotinylated anti-rat,actin and alpha and beta-tubulin for Western *** and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine *** 72 hours,the abundance of apyrases,cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in *** extracts were sharply increased than those sown in distilled *** the samples exhibited that the stems had more amount from apyrases,cytoskeletal proteins,amino acids and ATPase and ADPase activities than primary leaves and primary roots that were germinated either on *** water extract or in distilled *** on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins,*** water extract had an important effect on plant germinations.

关键词： Cytoskeletal proteins ATPase ADPase Pisum sativum Euryops arabicus

A review on zero energy buildings-Pros and cons

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Energy and Built Environment 2023年第1期4卷 25-38页

作者： Tabbi Wilberforce A.G.Olabi Enas taha sayed Khaled Elsaid Hussein M.Maghrabie Mohammad ali Abdelkareem Mechanical Engineering and Design Aston UniversitySchool of Engineering and Applied ScienceAston TriangleBirmingham B47ETUK Department of Sustainable and Renewable Energy Engineering University of SharjahP.O.Box 27272SharjahUnited Arab Emirates Center for Advanced Materials Research University of SharjahPO Box 27272SharjahUnited Arab Emirates Chemical Engineering Department Minia UniversityElminiaEgypt Chemical Engineering Department Texas A&M UniversityTX 77843-3122College StationTXUSA Department of Mechanical Engineering Faculty of EngineeringSouth Valley UniversityQena 83521Egypt

Enhancing the energy efficiency of structures has been a staple of energy *** key goal is to slash elec-tricity usage in order to minimize the footprint of *** goal is sought by putting restrictions on the design specifications with respect to the properties of the raw materials and components as well as the exploitation of sustainable sources of *** facts for the basis for zero-energy building(ZEB)being *** novel technology has faced several obstacles impeding its commercialization and future *** inves-tigation therefore holistically explored and evaluated the state of zero energy building and factors impeding their *** review further proposed some suggestion in terms of technology that can be considered by the sector to augment existing ***,the investigation touched on the effect of occupant’s character in zero energy *** in terms of government subsidies and tax rebates were recommended to encourage more investors into the ***,the perception of zero energy building being more expensive compared to the traditional structures can equally be curbed via efficient and effective public sensitization.

关键词： Zero energy buildings Policies Renewable energy Construction industry

Feasible design for electricity generation from Chlorella vulgaris using convenient photosynthetic conditions

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BIOCELL 2018年第1期42卷 7-11页

作者： MAHMOUD MOUSTAFA tarek taha MOHAMED ELNOUBY M.A.ABU-SAIED ali SHATI MOHAMED AL-KAHTANI SULAIMAN ALRUMMAN Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Composite and Nanostructured materials research department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt Polymer Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

Many recent studies are concerned with low cost,easy to handle and alternative renewable energy as a feasible solution for the upcoming crisis of energy *** are unicellular entities the can only depend on CO_(2),water and solar power to cover their nutritional *** current study is concerned with using algal cells in a polymeric hydrogel,as a cheap source of energy for electricity *** vulgaris has been proved to be a promising algal species for electricity generation,as compared with Micractinium *** hydrogel has been used for the immobilization of both algal species in order to protect them from the adverse surrounding conditions in addition to its ability to slowly release the required water molecules according to *** these conditions,*** showed the ability to generate 60 mV compared with 15 mV generated by *** electron micrographs showed nano-threads that bind the *** cells to each other,indicating the ability of algae to create nanowires that facilitate the electron transfer among algal cells and from cells to the nearest ***,we would expect an increase in the produced potential with simultaneous amendment of environmentally polluted water,such as sewage or waste *** of FTIR and raman spectroscopy proved the presence of the characteristic groups of PVA hydrogel and proved the proper integration of the algal cells inside the hydrogel cavities.

关键词： Chlorella vulgaris immobilization PVA hydrogel Electricity generation Algae Microbial fuel cell(MFC)

Progress in plant-based bioelectrochemical systems and their connection with sustainable development goals

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Carbon Resources Conversion 2021年第1期4卷 169-183页

作者： Enas taha sayed Mohammad ali Abdelkareem Khaled Obaideen Khaled Elsaid Tabbi Wilberforce Hussein M.Maghrabie A.G.Olabi Center for Advanced Materials Research University of SharjahPO Box 27272SharjahUnited Arab Emirates Chemical Engineering Department Minia UniversityElminiaEgypt Dept.of Sustainable and Renewable Energy Engineering University of SharjahP.O.Box 27272SharjahUnited Arab Emirates Chemical Engineering Department Texas A&M UniversityCollege StationTX 77843-3122TXUSA Mechanical Engineering and Design Aston UniversitySchool of Engineering and Applied ScienceAston TriangleBirmingham B47ETUK Department of Mechanical Engineering Faculty of EngineeringSouth Valley UniversityQena 83521Egypt

Living organisms’energy conversion is considered as an essential and sustainable green energy source and future bio-hybrid ***,plants were used after harvesting as biomass in bio-fermentation as an energy *** bio-electrochemical systems,microorganisms work with plants to generate electricity,hydrogen,or *** work discusses the simultaneous pollutant removal and electricity generation in plant-based bio-electrochemical systems(P-BES).Factors affecting the P-BES performance and the removal efficiencies of the different organic and inorganic pollutants were ***,the plant-based bioelectrochemical systems’role in achieving the sustainable development goals(SDGs)was *** SDGs contribution of plant-based bioelectrochemical systems were presented and discussed to evaluate such systems’ability to achieve the three pillars of sustainable development,i.e.,economic,environmental,and social.

关键词： Plant Bioelectrochemical systems Sustainable development goals,pollutant removal Power generation

Ultralong hydroxyapatite-based forward osmosis membrane for freshwater generation

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Frontiers of Chemical Science and Engineering 2024年第9期18卷 37-49页

作者： Mohamed Gamal Gomaa Hamdy Maamoun Abdel-Ghafar Francesco Galiano Francesca Russo Alberto Figoli El-sayed ali Abdel-Aal Abdel-Hakim taha Kandil Bahaa Ahmed Salah Central Metallurgical Research and Development Institute(CMRDI) 11421 CairoEgypt Chemistry Department Faculty of ScienceHelwan University11795 CairoEgypt Institute on Membrane Technology(CNR-ITM) 87036 RendeItaly

Increasing global water shortages are accelerating the pace of membrane manufacturing,which generates many environmentally harmful *** challenges need a radical rethink of developing innovative membranes that can address freshwater production without generating environmentally harmful *** work utilized the synthesized ultra-long hydroxyapatite(UHA)by the solvothermal method using the green solvent oleic acid in preparing UHA-based forward osmosis *** membranes were developed using different loading ratios of graphene oxide(GO)by vacuum-assisted filtration *** prepared GO/UHA membranes were identified using X-ray diffraction,scanning electron microscope,Fourier-transform infrared spectroscopy,and X-ray photoelectron *** contact angle and pore size distribution were determined for the obtained GO/UHA *** obtained hierarchical porous structure in the prepared membranes with interconnected channels results in a stable water flux with reverse salt *** best water flux rate of 42±2 L·m^(–2)·h^(–1)was achieved using the 50 mg GO/UHA membrane,which is 3.3 times higher than the pristine membrane,and a reverse salt flux of 67 g·m^(-2)·h^(–1).The obtained results showed a promising capability of a new generation of sustainable inorganic-based membranes that can be utilized in freshwater generation by energy-efficient techniques such as forward osmosis.

关键词： forward osmosis ultra-long hydroxyapatite graphene oxide inorganic-based membrane

Hydrothermal preparation of TiO_(2)-Ag nanoparticles and its antimicrobial performance against human pathogenic microbial cells in water

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BIOCELL 2018年第3期42卷 93-97页

作者： Mahmoud MOUSTAFA Saad ALAMRI Mohamed ELNOUBY tarek taha M.A.ABU-SAIED ali SHATI Mohamed AL-KAHTANI Sulaiman ALRUMMAN Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Composite and Nanostructured Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-CITY)New Borg El-Arab City21934AlexandriaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-CITY)New Borg El-Arab City21934AlexandriaEgypt Polymer Materials Research Department Advanced Technology and New Materials Research InstituteCity of Scientific Research and Technological Applications(SRTA-CITY)New Borg El-Arab City21934AlexandriaEgypt

Water contaminated with pathogenic microbes is considered as one of the most common routes for transmitting diseases in human *** methods have been applied for the decontamination of microbes in contaminated *** the current study,an easy to do hydrothermal method has been used for the preparation of TiO_(2)-Ag *** obtained material was characterised using a scanning electron microscope(SEM)and fourier transform infra-red spectroscopy(FTIR).The morphological appearance of the obtained nanoparticles was in the shape of a sphere with a size range of 60-90 *** antimicrobial activity of the prepared nanoparticles was tested against several pathogenic bacteria and *** obtained results proved that the nanoparticles succeeded to affect all the tested microbes in the following order:Bacillus cereus ATCC6633>Pseudomonas aeruginosa ATCC9027=Klebsiella pneumoniae ATCC13883>Vibrio cholera ATCC700=Candida albicans ATCC 700=Escherichia coli NCTC10418>Staphylococcus aureus *** minimum inhibitory concentration(MIC)of the prepared nanoparticles varied among the tested microbes at range of 12 mg/ml and 25 mg/*** results encourage the application of prepared TiO_(2)-Ag nanoparticles for treatment of microbe-contaminated waters.

关键词： TiO_(2)-Ag nanoparticles Hydrothermal Antimicrobial activity Water treatment

Meta-heuristics for Feature Selection and Classification in Diagnostic Breast Cancer

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Computers, Materials & Continua 2022年第10期73卷 749-765页

作者： Doaa Sami Khafaga Amel ali Alhussan El-sayed M.El-kenawy ali E.Takieldeen tarek M.Hassan Ehab A.Hegazy Elsayed Abdel Fattah Eid Abdelhameed Ibrahim Abdelaziz A.Abdelhamid Department of Computer Sciences College of Computer and Information SciencesPrincess Nourah Bint Abdulrahman UniversityRiyadh11671Saudi Arabia Department of Communications and Electronics Delta Higher Institute of Engineering and TechnologyMansoura35111Egypt Faculty of Artificial Intelligence Delta University for Science and TechnologyMansoura35712Egypt Communications and Computers Engineering Department Faculty of EngineeringDelta University for Science and TechnologyInternational Coastal RoadGamasa CityDakhliyaEgypt Faculty of Applied Health Science Technology Delta University for Science and TechnologyMansoura35712Egypt Faculty of Medicine Head of Internal Medicine DepartmentDelta University for Science and TechnologyMansouraEgypt Computer Engineering and Control Systems Department Faculty of EngineeringMansoura University35516MansouraEgypt Department of Computer Science Faculty of Computer and Information SciencesAin Shams University11566CairoEgypt Department of Computer Science College of Computing and Information TechnologyShaqra University11961Saudi Arabia

One of the most common kinds of cancer is breast *** early detection of it may help lower its overall rates of *** this paper,we robustly propose a novel approach for detecting and classifying breast cancer regions in thermal *** proposed approach starts with data preprocessing the input images and segmenting the significant regions of *** addition,to properly train the machine learning models,data augmentation is applied to increase the number of segmented regions using various scaling *** the other hand,to extract the relevant features from the breast cancer cases,a set of deep neural networks(VGGNet,ResNet-50,AlexNet,and GoogLeNet)are *** resulting set of features is processed using the binary dipper throated algorithm to select the most effective features that can realize high classification *** selected features are used to train a neural network to finally classify the thermal images of breast *** achieve accurate classification,the parameters of the employed neural network are optimized using the continuous dipper throated optimization *** results show the effectiveness of the proposed approach in classifying the breast cancer cases when compared to other recent approaches in the ***,several experiments were conducted to compare the performance of the proposed approach with the other *** results of these experiments emphasized the superiority of the proposed approach.

关键词： Breast cancer image segmentation dipper throated optimization feature selection meta-heuristics