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Immobilization in Spheres of a Cocktail Rich in Xylanase Produced by the Fungus <i>Fusarium sp</i>. EA 1.3.1 for Hydrolysis of Sugarcane Bagasse

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Advances in Bioscience and Biotechnology 2021年第12期12卷 436-457页

作者： Alice Gomes Miranda tarcisio michael ferreira soares de oliveira Rosymar Coutinho de Lucas David Lee Nelson Juan Pedro Bretas Roa Vivian Machado Benassi Programa de Pós-Graduaç ã o em Biocombustíveis Universidade Federal dos Vales do Jequitinhonha e Mucuri campus JK Diamantina Minas Gerais Brazil Instituto de Ciê ncia e Tecnologia Universidade Federal dos Vales do Jequitinhonha e Mucuri campus JK Diamantina Minas Gerais Brazil Instituto de Ciê ncias Biológicas Departamento de Parasitologia Microbiologia e Imunologia Universidade Federal de Juiz de Fora Minas Gerais Brazil Faculdade de Medicina de Ribeirã o Preto Departamento de Bioquímica Universidade de S&#227 o Paulo Ribeir&#227 o Preto S&#227 o Paulo Brazil

Second generation ethanol is produced from the degradation of lignocellulosic biomass using enzymes as catalysts, with emphasis on xylanases. These biocatalysts are often costly, but stable at high temperatures, and their reuse is of great value, so the immobilization of the enzymes can increase their applicability on an industrial scale. We sought to immobilize a cocktail rich in xylanase produced by the fungus Fusarium sp. EA 1.3.1 in alginate spheres, optimize the immobilization method, characterize the immobilized derivatives, improve their physical-chemical characteristics, and perform the hydrolysis of sugarcane bagasse to release sugars. The Fusarium sp. EA 1.3.1 has been identified and used for cocktail rich in xylanase production that was immobilized in alginate spheres. During this process, the drip equipment, and the concentration of the solutions of sodium alginate and calcium chloride were evaluated. The best results were obtained with the glass rod and with concentrations of 3.14% and 2.10% for the solutions, respectively. The apparent optimum conditions of pH and temperature reaction were studied, and the values of pH 6.5 and 60°C were obtained. The immobilized conjugate also presented greater stability at this temperature than that of the soluble cocktail. The conjugate could be recycled up to six times, and its activity was maintained after 75 days of storage. Finally, the hydrolysis in natural sugarcane bagasse was achieved, and greater amounts of reducing sugars were obtained in the reaction with the conjugate. Thus, the cocktail rich in xylanase produced by the fungus Fusarium sp. EA1.3.1 was successfully immobilized on alginate spheres and possesses the potential to be used as a catalyst in industrial processes such as the lignocellulosic ethanol industry.

关键词： Xylanase Cocktail Spheres Immobilization Lignocellulolytic Enzymes Biomass Hydrolysis

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Isolation of Filamentous Fungi from the Caatinga Region and Production of Amylolytic Enzymes of Great Industrial Interest

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Journal of Biosciences and Medicines 2020年第11期8卷 152-164页

作者： Ingrid Cristina soares Amorim Gessica oliveira Marinho tarcisio michael ferreira soares de oliveira Juan Pedro Bretas Roa Arlete Barbosa dos Reis David Lee Nelson Thiago Machado Pasin Vivian Machado Benassi Instituto de Ciência e Tecnologia Universidade Federal dos Vales dos Jequitinhonha e Mucuri Campus JK Diamantina Brasil Programa de Pós-Graduaç &atilde o em Biocombustíveis Universidade Federal dos Vales dos Jequitinhonha e Mucuri Campus JK Diamantina Brasil departamento de Bioquímica e Imunologia Faculdade de Medicina de Ribeir&atilde o Preto Universidade de S&atilde o Paulo Ribeir&atilde o Preto Brasil

The industrial importance and the high cost of the commercial amylase require the study of microorganisms that produce these enzymes. For this reason, the objective of this work was to isolate filamentous fungi from a region of the caatinga and evaluate their potential for the production of amylase. Four soil samples were collected from a deactivated dump located in the city of Diamantina, MG, in a region of the caatinga. The analysis of amylolytic production in a submerged medium at the ideal temperature of each microorganism was performed using the saccharification method, and the reducing sugars formed were quantified by DNS. Fourteen filamentous fungi were isolated, which had different morphological aspects. Regarding amylase production, a mean activity of 0.477 U·mL-1 was obtained with the isolates I 1.2.1 and I 4.4.1. These results bring important information regarding the biodiversity of the caatinga, in addition to the isolation of microorganisms that can be used as biological machinery to obtain metabolites with high biotechnological and industrial potential.

关键词： Amylolytic Enzyme Bioprospecting Filamentous Fungi Industrial Interest

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Histological Assessment of Bone Regeneration in the Maxilla with Homologous Bone Graft:A Feasible Option for Maxillary Bone Reconstruction

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Journal of Renewable Materials 2024年第1期12卷 131-148页

作者： Sergio Henrique Gonçalves Motta Ana Paula Ramos soares Juliana Campos Hasse Fernandes Gustavo Vicentis oliveira Fernandes Graduate Center of Faculdade Redentor CLIVORio de JaneiroBrazil Periodontics and Oral Medicine department University of Michigan School of DentistryAnn ArborMI48109USA Centre for Interdisciplinary Research in Health(CIIS) Universidade Católica PortuguesaViseu3504-505Portugal

Bone biomaterials have been increasingly used to reconstruct maxillary atrophic ***,the aim of this study was to evaluate bone reconstruction in the maxilla using a homologous cortico-cancellous FFB(lyophilized)graft and verify its *** individuals were included from 2014 to *** first surgery was performed to install homologous bone blocks in the *** period of the second intervention varied between 5 months and 15 days to 11 months(≈7.93 months).The biopsies were taken from the central region of the matured graft during the surgery for implant *** patients presented clinical and radiographic conditions for the installation of dental *** was a 100%of survival *** histological assessment showed that the homologous block bone graft was an osteoconductive biomaterial,with connective tissue present,and newly formed bone juxtaposed on its *** were bone trabeculae with osteocytes and active osteoblasts with connective tissue in the mineralization process;the remodeling process can be found through the reverse lines.A limited focus of necrosis with fibrosis was detected,with small resorption and areas of inflammatory infiltrate,but without clinical *** homologous block bone graft can be considered a feasible option to substitute the autogenous bone graft(gold standard),with predictable clinical and favorable histological *** patients had a shorter surgical period,low morbidity,and an unlimited amount of biomaterial available at an accessible cost.

关键词： Regeneration bone graft histology homologous allogenous autogenous

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Liquid crystal immunosensors for the selective detection of Escherichia coli with a fast analysis tool

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Photonics Research 2024年第7期12卷 1564-1573页

作者： SANDRO C.oliveira MARIA S.soares BÁRBARA V.GONÇALVES ANDREIA C.M.RODRIGUES AMAdeU M.V.M.soares RITA G.SOBRAL NUNO F.SANTOS JAN NEDOMA PEDRO L.ALMEIDA CARLOS MARQUES CICECO-Aveiro Institute of Materials Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal i3N Physics DepartmentUniversity of Aveiro3810-193 AveiroPortugal Associate Laboratory i4HB-Institute for Health and Bioeconomy School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal CESAM-Centre for Environmental and Marine Studies and department of Biology University of Aveiro3810-193 AveiroPortugal department of Telecommunications VSB-Technical University of OstravaOstrava 70800Czech Republic i3N-CENIMAT School of Sciences and TechnologyNOVA University of Lisbon1099-085 LisbonPortugal UnIRE ISELPolytechnic Institute of Lisbon1549-020 LisbonPortugal department of Physics VSB-Technical University of OstravaOstrava 70800Czech Republic

The consumption of contaminated food may cause serious illnesses,and traditional methods to detect Escherichia coli are still associated with long waiting times and high costs given the necessity to transport samples to specialized *** is a need to develop new technologies that allow cheap,fast,and direct monitoring at the site of ***,in this work,we developed optical immunosensors for the selective detection of ***,based on liquid crystal technology,whose molecules can align in different manners depending on the boundary conditions (such as substrates) as well as the environment that they *** glass substrate was functionalized with *** antibody using cysteamine as an intermediate,and a vertical alignment was imposed on the liquid crystal molecules by using DMOAP during *** presence of bacteria disrupts the alignment of the liquid crystal molecules,changing the intensity of light emerging between cross polarizers,measured using a polarized optical microscope and a *** was possible to detect *** in suspensions in the concentration range from 2.8 cells/mL to 2.8×10^(9) cells∕*** was also evaluated,and the sensors were used to analyze contaminated water samples.A prototype was developed to allow faster,in-situ,and easier analysis avoiding bulky instruments.

关键词： crystal alignment analysis

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Risk Stratification in Obstetrics: An Integrated Approach to Maternal Health

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Open Journal of Obstetrics and Gynecology 2024年第6期14卷 903-910页

作者： Taina Crespo Mendonca Bianca Beatriz soares dos Reis Júlia Cruz Coelho Antônio de oliveira Battistini Pestana Vitória Carolina Barreto Negri Alvaro Augusto Trigo department of Medicine University of Franca Franca Brazil

Introduction: Prenatal care is crucial for maternal and fetal health, highlighting the importance of obstetric risk stratification. This enables personalized care, avoiding unnecessary interventions and reducing costs. Conditions such as first trimester bleeding, spontaneous abortion, gestational trophoblastic disease, and ectopic pregnancy require specific approaches. Early identification of these complications is vital, especially in urgent and emergency obstetric cases, which demand immediate hospital attention. Objective: Comprehensive review of first trimester pathologies and gestational hypertension, focusing on obstetric risks and personalized prenatal management. Methods: Literature review on bleeding and gestational hypertension. Results: The study provides a detailed analysis of pathologies associated with first trimester uterine bleeding and hypertensive disorders, focusing on obstetric risk stratification. The main causes of maternal and fetal mortality are hypertensive disorders, hemorrhages, infections, childbirth complications, and abortion. Spontaneous abortion is common, with different classifications, ranging from threatened to missed abortion. Infected abortion is a severe complication. Brazil has restrictive abortion laws, but many unsafe abortions occur, resulting in high public health costs. Pharmacological treatment with misoprostol is a safe option. Other pathologies include gestational trophoblastic disease, ectopic pregnancy, and placenta previa. Preeclampsia and eclampsia are severe conditions requiring immediate treatment to avoid complications. Conclusion: Early identification and management of obstetric risk factors, such as uterine bleeding, are essential for positive maternal and fetal outcomes. A multidisciplinary approach is fundamental.

关键词： Obstetrics Emergencies Hypertension Pregnancy-Induced Uterine Hemorrhage Pregnancy Trimester First

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development of a Ready-to-Use PSMA-11 Kit Formulation and Biological Evaluation of Binding Affinity

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Advances in Molecular Imaging 2024年第2期13卷 7-22页

作者： Maria Renata Valente Brandão Freire Natália Marchesan Bexiga Marco Antonio Stephano João Ezequiel oliveira Carlos Roberto Jorge soares Ana Cláudia Camargo Miranda Anna Carolina de Alcântara Barbosa Marycel Figols de Barboza Laura Fernanda Garcia Luiz Felipe S. Teixeira Maria Helena Marumo Bellini Natanael Gomes da Silva Luiza Mascarenhas Balieiro Joel Mendes Elaine Bortoleti de Araújo Centro de Radiofarmcia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil departamento de Tecnologia Farmacutica Faculdade de Cincias Farmacuticas (USP) Cidade Universitria So Paulo Brasil Centro de Biotecnologia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil departamento de Medicina Nuclear do Hospital Israelita Albert Einstein So Paulo/SP Brasil

Introduction: 68Ga-PSMA-11 is considered the gold standard in detection of micro and oligometastases in advanced prostate cancer, being used for therapeutic planning, as well as, potentially, for evaluating response to treatment. The development of ready-to-use lyophilized kit of PSMA-11 adds quality and safety to the routine use of this radiopharmaceutical and represents a pharmacotechnical challenge as it must preserve the integrity and specificity of the ligand. Methods: PSMA-11 kit formulation was proposed, considering radiolabeling parameters and the preservation of the peptide during the lyophilization process, using mannitol as an excipient. Critical temperature characterization studies were carried out using DSC equipment and the freeze-drying process was developed. The direct radiolabeling conditions were evaluated and standardized using 68Ge/68Ga generator eluate from two different manufacturers (ITG and Eckert & Ziegler). The radiochemical purity was evaluated by TLC and HPLC. Biological evaluation was carried out with lyophilized PSMA-11 to demonstrate the integrity of the peptide and preservation of biological activity after the lyophilization process. Results: Based on critical temperature characterization studies, the freeze-drying cycle was designed to reach a freezing temperature of around −40˚C and primary drying at 2˚C. Using 20 mg of mannitol, an intact and elegant lyophilized cake was obtained. PSMA-11 lyophilized kit was directly labeled with 68Ga eluate from 68Ge/68Ga GMP generators (ITG and Eckert & Ziegler) resulting in % RP > 95% at pH 4.0 to 4.5. The results obtained from in vitro and in vivo biological competition studies confirmed the preservation of PSMA-11 affinity for the receptor after lyophilization. Conclusion: A lyophilized formulation (Kit) of PSMA-11 was successfully obtained, which preserved the integrity and biological activity of the peptide and guaranteed radiolabeling efficiency.

关键词： 68Ga-PSMA-11 Prostate-Specific Membrane Antigen (PSMA) Ready-to-Use Kit GMP-Grade Generators Metastatic Prostate Cancer PET Imaging

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国际护理知识网络建设的调查研究

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International Journal of Nursing Sciences 2024年第3期11卷 374-380,I0004,I0005页

作者： Margareth Santos Zanchetta Suzanne Fredericks Kateryna Metersky Geraldine Martorella Pammla Petrucka Laurie Clune Kelly Graziani Giacchero Vedana Cristina Rosa soares Lavareda Baixinho Cristianne Maria Famer Rocha Sara Campagna Sally Zhang He Márcia Teles de oliveira Gouveia Marcelo Medeiros denize Bouttelet Munari Daniel Gonzalo Eslava Albarracin Carlos Aguilera-Serrano Walterlania Silva Santos Toronto Metropolitan University TorontoCanada Florida State University TallahasseeUSA University of Saskatchewan ReginaCanada University of Regina ReginaCanada State of Sao Paulo University Ribeirao PretoBrazil Lisbon Superior School of Nursing LisbonPortugal Federal University of Rio Grande do Sul Porto AlegreBrazil University of Turin TurinItaly Scarborough Health Network ScarboroughCanada Federal University of Piauí TeresinaBrazil Federal University of Goias GoianiaBrazil Fundacion Universitaria Cafam BogotaColombia University of Malaga MalagaSpain University of Brasília Ceil^andiaBrazil

本文报告了为创建护理知识网络(Nursing Knowledge Network,NKN)对不同国家护理院校进行环境扫描调查所收集到的信息。采用在线方式(2021年2-10月)进行环境扫描,探索研究领域的确定、现有资源、预期收益、教学研究创新、知识传播障碍及... 详细信息

本文报告了为创建护理知识网络(Nursing Knowledge Network,NKN)对不同国家护理院校进行环境扫描调查所收集到的信息。采用在线方式(2021年2-10月)进行环境扫描,探索研究领域的确定、现有资源、预期收益、教学研究创新、知识传播障碍及网络的预期贡献。目标信息提供者包括来自13个国家63所大学的200名护理教师。要求每所大学有1名参与者回答问卷。共有42所大学(约70%)回复,包括巴西21所,加拿大4所,葡萄牙和西班牙各3所,哥伦比亚、墨西哥、秘鲁和美国各2所,智利、意大利和巴拉圭各1所。其分享了相关信息和见解:通过护理高等教育机构之间的协作和交流,全球可以加强共同创新和研究合作能力;较少被研究的领域是知识网络生成证据、为当地科学实践提供信息的潜在重点。这些信息将为进一步了解护理发展,以及地方、区域和全球护理证据应用和传播相关的政府政策与计划制订提供参考。

关键词：信息网络创新扩散国际教育交流知识管理护理教育护理教师实践护理院校跨文化护理

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Time-dependent impact of a high-fat diet on the intestinal barrier of male mice

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World Journal of Methodology 2024年第1期14卷 114-123页

作者： Carolline Santos Miranda Daiana Araujo Santana-oliveira Isabela Lopes Vasques-Monteiro Nathan soares Dantas-Miranda Jade Sancha de oliveira Glauser Flavia Maria Silva-Veiga Vanessa Souza-Mello department of Anatomy Rio de Janeiro State UniversityRio de Janeiro 20551030Brazil

BACKGROUND Excessive saturated fat intake compromises the integrity of the intestinal mucosa,leading to low-grade inflammation,impaired mucosal integrity,and increased intestinal permeability,resulting in the migration of lipopolysaccharide(LPS)to other *** To evaluate the chronic effects(at 10 and 16 wk)of a high-fat diet(HFD)(with 50%energy as fat)on the phylogenetic gut microbiota distribution and intestinal barrier structure and protection in C57BL/6 *** Forty adult male mice were divided into four nutritional groups,where the letters refer to the type of diet(control and HFD or HF)and the numbers refer to the period(in weeks)of diet administration:Control diet for 10 wk,HFD for 10 wk,control diet for 16 wk,and HFD for 16 *** sacrifice,biochemical,molecular,and stereological analyses were *** The HF groups were overweight,had gut dysbiosis,had a progressive decrease in occludin immunostaining,and had increased LPS *** progression reduced the number of goblet cells per large intestine area and Mucin2 expression in the HF16 group,consistent with a completely disarranged intestinal ultrastructure after 16 wk of HFD *** Chronic HFD intake causes overweight,gut dysbiosis,and morphological and functional alterations of the intestinal barrier after 10 or 16 ***-dependent reductions in goblet cell numerical density and mucus production have emerged as targets for countering obesity-driven intestinal damage.

关键词： High-fat diet Intestine Ultrastructure Goblet cells Gut microbiota

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Behavior of wood basic density according to environmental variables

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Journal of Forestry Research 2022年第2期33卷 497-505页

作者： Gabriel Marcos Vieira oliveira JoséMárcio de Mello Carlos Rogério de Mello JoséRoberto soares Scolforo Eder Pereira Miguel Thiago Campos Monteiro Brazil Institute of Agricultural and Forestry defense of Espírito Santo(IDAF) Av.Lourival Lugon Moulinnº300CentroJerônimo MonteiroES CEP:29.550-000Brazil department of Forestry Sciences Universidade Federal de Lavras-UFLACaixa Postal 3037LavrasMG CEP:37.200-000Brazil Engineering department Universidade Federal de Lavras-UFLACaixa Postal 3037LavrasMG CEP:37.200-000Brazil Forestry Engineering department Faculdade de TecnologiaUniversity of Brasilia-UnBCampus Universitário Darcy RibeiroCaixa Postal 4357Asa Norte-BrasíliaDF CEP:70910-900Brazil department of Forestry Engineering and Technology Universidade Federal do Paraná-UFPRAv.Pref.Lothário Meissner632Jardim BotânicoCuritibaPR CEP:80.210-170Brazil

The relationships between climate conditions and wood density in tropical forests are still poorly *** quantify spatial dependence of wood density in the state of Minas Gerais(MG,Brazil),map spatial distribution of density,and correlate density with climate variables,we extracted data from the Forest Inventory of Minas Gerais for 1988 trees scaled throughout the territory and measured wood density of discs removed from the *** variables were extracted from the database of the Ecological-Economic Zoning of Minas *** spatial analysis,tree densities were measured at 44 georeferenced sampling *** data were subjected to exploratory analysis,variography,cross-validation,model selection,and ordinary *** relationships between wood density and environmental variables were calculated using dispersion matrices,linear correlation,and *** density proved to be highly spatially dependent,reaching a correlation of 96%,and was highly continuous over a distance of 228 *** distribution of wood density followed a continuous gradient of 514-659 kg m^(−3),enabling corre-lation with environment *** was correlated with mean annual precipitation(−0.57),temperature(0.63),and evapotranspiration(0.83).Geostatistical methods proved useful in predicting wood density in native tropical forests with different climate *** results confirmed the sensitivity of wood density to climate change,which could affect future carbon stock in forests.

关键词： Biomass Climate variables Geostatistics Hardwood Forest inventory Minas Gerais

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dealing with Multisource Information for Estuarine Flood Risk Appraisal in Two Western European Coastal Areas

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International Journal of Disaster Risk Science 2022年第2期13卷 199-213页

作者： Ana Rodrigues Rilo Alexandre Manuel de oliveira soares Tavares Paula Maria dos Santos Freire José Luis Zêzere Estuarine and Coastal Zones Unit LNEC-National Civil Engineering Laboratory.1700-075 LisbonPortugal Centre of Geographical Studies Institute of Geography and Spatial PlanningUniversidade de Lisboa1700-066 LisbonPortugal Centre for Social Studies and Earth Sciences department Coimbra University3030-790 CoimbraPortugal

Estuaries are usually affected by compound flooding triggers that cause diverse territorial *** fluvial flood risk assessment frameworks are well established in the literature,integrated management instruments that deal with estuarine flood risk remain incomplete and often *** research presents a methodology to extract relevant information from multiple sources post-event and a database building process that is applied to two contrasting estuaries(the Tagus River estuary in Portugal,and the Shannon River estuary in Ireland)in the Western European coastal ***,a total of 274 documents were analyzed and the information was stored in two *** correspondence analysis was applied to extract the most informative and relevant estuarine flood *** integrated estuarine flood risk assessment framework is presented and discussed based on the extracted *** framework is driven by two distinct dimensions(oceanic and hydrographic)and revealed the transversal position of triggers of estuarine floods,reflecting the compounding effects usually present in these *** results also highlight two levels of flood risk mostly based on damage typology.

关键词： Estuarine floods Estuarine flood indicators Multiple correspondence analysis Post-hazard multisource information Western Europe

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