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In vitro pancreas duodenal homeobox-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells

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World Journal of Gastroenterology 2007年第39期13卷 5232-5237页

作者： tao liu chun-you wang feng yu shan-miao gou he-shui wu jiong-xin xiong feng zhou Department of Pancreatic Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM: To observe whether pancreatic and duodenal homeobox factor-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells in vitro. METHODS: Rat pancreatic tissue was submitted to digestion by collegenase, ductal epithelial cells were separated by density gradient centrifugation and then cultured in RPMI1640 medium with 10% fetal bovine serum. After 3-5 passages, the cells were incubated in a six-well plate for 24 h before transfection of recombination plasmid XlHbox8VP16. Lightcycler quantitative real-time RT-PCR was used to detect the expression of PDX-1 and insulin mRNA in pancreatic epithelial cells. The expression of PDX-1 and insulin protein was analyzed by Western blotting. Insulin secretion was detected by radioimmunoassay. Insulin- producing cells were detected by dithizone-staining. RESULTS: XlHbox8 mRNA was expressed in pancreatic ductal epithelial cells. PDX-1 and insulin mRNA as well as PDX-1 and insulin protein were signifi cantly increased in the transfected group. The production and insulin secretion of insulin-producing cells differentiated from pancreatic ductal epithelial cells were higher than those of the untransfected cells in vitro with a significant difference (1.32 ± 0.43 vs 3.48 ± 0.81, P helial cells into insulin-producing cells in vitro. In vitro PDX-1 transfection is a valuable strategy for increasing the source of insulin-producing cells.

关键词： Pancreatic ductal epithelial cells Islet Differentiation Pancreas duodenal homeobox-i

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Pancreas duodenal homeobox-1 expression and significance in pancreatic cancer

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World Journal of Gastroenterology 2007年第18期13卷 2615-2618页

作者： tao liu shan-miao gou chun-you wang he-shui wu liong-xin xiong feng zhou Department of Pancreatic Surgery Union Hospital Tongji Medical College Huazhong University ofScience and Technology Wuhan 430022 Hubei Province China

AIM： To study the correlations of Pancreas duodenal homeobox-1 with pancreatic cancer characteristics, including pathological grading, TNM grading, tumor metastasis and tumor cell proliferation. METHODS： Reverse transcriptase-polymerase chain reaction （RT-PCR） was used to detect PDX-1 mRNA expression in pancreatic cancer tissue and normal pancreatic tissue. The expression of PDX-1 protein was measured by Western blot and immunohistochemistry. Immunohistochemistry was also used to detect proliferative cell nuclear antigen （PCNA）. Correlations of PDX-1 with pancreatic cancer characteristics, including pathological grading, TNM grading, tumor metastasis and tumor cell proliferation, were analyzed by using χ^2 test. RESULTS： Immunohistochemistry showed that 41.1% of pancreatic cancers were positive for PDX-1 expression, but normal pancreatic tissue except islets showed no staining for PDX-1. In consistent with the result of imunohistochemistry, Western blot showed that 37.5% of pancreatic cancers were positive for PDX-1. RT-PCR showed that PDX-1 expression was significantly higher in pancreatic cancer tissues than normal pancreatic tissues （2^-3.56±0.35 vs 2^-8.76±0.14, p 〈 0.01）. Lymph node metastasis （P 〈 0.01）, TNM grading （P 〈 0.05）, pathological grading （P 〈 0.05） and tumor cell proliferation （P 〈 0.01） were significantly correlated with PDX-1 expression levels. CONCLUSION： PDX-1 is re-expressed in pancreatic cancer, and PDX-l-positive pancreatic cancer cells show more malignant potential compared to PDX-l-negative cells. Therefore, PDX-l-positive cells may be tumor stemcells and PDX-1 may act as alternate surface marker of pancreatic cancer stem cells.

关键词： Pancreatic neoplasms Transcription factors Tumor stem cells Lymphatic metastasis Biological markers

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Persistence of side population cells with high drug efflux capacity in pancreatic cancer

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World Journal of Gastroenterology 2008年第6期14卷 925-930页

作者： Jing zhou chun-you wang tao liu Bin wu feng zhou jiong-xin xiong he-shui wu Jing tao Gang Zhao Ming Yang shan-miao gou Institute of Pancreatic Surgery Union Hospital Huazhong University of Science and Technology 1277 Jiefang Ave. Wuhan 430022 Hubei Province China Department of Orthopaedics Union Hospital Huazhong University of Science and Technology 1277 Jiefang Ave Wuhan 430022 Hubei Province China

AIM: To investigate the persistence of side population (SP) cells in pancreatic cancer and their role and mechanism in the drug resistance. METHODS: The presentation of side population cells in pancreatic cancer cell line PANC-1 and its proportion change when cultured with Gemcitabine, was detected by Hoechst 33342 staining and FACS analysis. The expression of ABCB1 and ABCG2 was detected by real- time PCR in either SP cells or non-SP cells. RESULTS: SP cells do exist in PANC-1, with a median of 3.3% and a range of 2.1-8.7%. After cultured with Gemcitabine for 3 d, the proportion of SP cells increased significantly (3.8% ± 1.9%, 10.7% ± 3.7%, t = 4.616, P = 0.001 her concentrations in SP as compared with non-SP cells (ABCB1: 1.15 ± 0.72, 5.82 ± 1.16, t = 10.839, P = 0.000 he efflux of fluorescent staining and drug resistance. CONCLUSION: SP cells with inherently high resistance to chemotherapeutic agents do exist in pancreatic cancers, which may be candidate cancer stem cells contributing to the relapse of the tumor.

关键词： Side population cells Hoechst 33342 Drug resistance Cancer stem cells Pancreatic cancer

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保留十二指肠的胰头全切术治疗慢性胰腺炎35例报告

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中华普通外科杂志 2009年第3期24卷 179-181页

作者：周峰王春友吴河水熊炯所刘涛勾善淼华中科技大学附属协和医院胰腺外科湖北武汉430022

目的总结保留十二指肠的胰头全切术治疗慢性胰腺炎的经验。方法回顾分析自1999年1月至2006年12月采用保留十二指肠的胰头全切术治疗慢性胰腺炎35例的临床资料，并对患者疼痛改善情况及内分泌功能变化进行随访。结果平均手术时间为286±5... 详细信息

目的总结保留十二指肠的胰头全切术治疗慢性胰腺炎的经验。方法回顾分析自1999年1月至2006年12月采用保留十二指肠的胰头全切术治疗慢性胰腺炎35例的临床资料，并对患者疼痛改善情况及内分泌功能变化进行随访。结果平均手术时间为286±55min，平均手术输血量为1.4±1.3单位压积红细胞。无手术死亡。术后并发症发生率为17％，其中1例胰瘘，3例胆漏，1例腹腔出血，1例切口裂开，无十二指肠瘘。术后患者疼痛得到缓解，QLQ-C30疼痛评分由术前的59±27降至术后的13±21。术后患者内分泌功能未继续恶化，随访过程中无新增糖尿病病例出现。结论保留十二指肠的胰头全切术是一种安全、有效的治疗慢性胰腺炎的手术方式。较之保留十二指肠的胰头次全切除术，本手术能更彻底的切除胰头和钩突的炎性病变。

关键词：胰十二指肠切除术胰腺炎手术后并发症

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