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Effect of human autologous serum and fetal bovine serum on human corneal epithelial cell viability,migration and proliferation in vitro

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 908-913页

作者： Ming-Feng Wu tanja stachon1 Berthold Seitz Achim Langenbucher Nora Szentmary Department of Ophthalmology Saarland University Medical Center Experimental Ophthalmology Saarland University Department of Ophthalmology Semmelweis University

AIM： To analyze the concentration-dependent effects of autologous serum （AS） and fetal bovine serum （FBS） on human corneal epithelial cell （HCEC） viability, migration and proliferation. METHODS： AS was prepared from 13 patients with non- healing epithelial defects Dulbecco＇s modified eagle medium/ Ham＇s F12 （DMEM/F12） with 5% FBS, 0.5% dimethyl sulphoxide （DMSO）, 10 ng/mL human epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in serum media： DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS or FBS. HCEC viability was analyzed using cell proliferation kit XTI＇, migration using a wound healing assay, proliferation by the cell proliferation enzyme-linked immunosorbent assay （ELISA） BrdU kit. Statistical analysis was performed using the generalized linear model, the values at 30% AS or 30% FBS were used as the baselines. RESULTS： HCEC viability was the highest at 30% AS or 15% FBS and the lowest at 10% AS or 30% FBS application. HCEC migration was the quickest through 30% AS or 30% FBS and the slowest through 5% AS or 5% FBS concentrations. Proliferation was the most increased through 15% AS or 5% FBS and the least increased through 30% AS or 30% FBS concentrations. HCEC viability at 10% and 15% AS was significantly worse （P=0.001, P=0.023） compared to baseline and significantly better at 15% FBS （P=0.003） concentrations. HCEC migration was significantly worse （P〈0.007） and HCEC proliferation significantly better （P〈0.001） in all concentration groups compared to baseline. CONCLUSION： For the best viability of HCEC 30% AS or 15% FBS, for HCEC migration 30% AS or 30% FBS, for proliferation 15% AS or 5% FBS should be used. Therefore, we suggest the use of 30% AS in clinical practice.

关键词： autologous serum eye drops serum concen-tration migration proliferation viability human comeal epithelialcells

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Impact of crosslinking/riboflavin-UVA-photodynamic inactivation on viability,apoptosis and activation of human keratocytes in vitro

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The Journal of Biomedical Research 2015年第4期29卷 321-325页

作者： tanja stachon Jiong Wang Xufei Song Achim Langenbucher Berthold Seitz Nóra Szentmáry Department of Ophthalmology Saarland University Medical Center Department of Ophthalmology Renmin Hospital of Wuhan University Department of Ophthalmology The Second Hospital of Jilin University Experimental Ophthalmology Saarland University

Riboflavin-UVA photodynamic inactivation is a potential treatment altemative in therapy resistant infectious keratitis. The purpose of our study was to determine the impact of riboflavin-UVA photodynamic inactivation on viability, apop- tosis and activation of human keratocytes in vitro. Primary human keratocytes were isolated from human corneal buttons and cultured in DMEM/Ham＇s F12 medium supplemented with 10% fetal calf serum. Keratocytes underwent UVA light illumination （375 nm） for 4.10 minutes （2 J/cm2） during exposure to different concentrations of riboflavin. Twenty-four hours after treatment, cell viability was evaluated photometrically, whereas apoptosis, CD34 and alpha-smooth muscle actin （α-SMA） expression were assessed using flow cytometry. We did not detect significant changes in cell viability, apoptosis, CD34 and α-SMA expression in groups only treated with riboflavin or UVA light. In the group treated with riboflavin-UVA-photodynamic inactivation, viability of keratocytes decreased significantly at 0.1% riboflavin （P〈0.01） while the percentage of CD34 （P〈0.01 for both 0.05% and 0.1% riboflavin） and alpha-SMA positive keratocytes （P〈0.01 and P〈0.05 for 0.05% and 0.1% riboflavin, respectively） increased significantly compared to the controls. There was no significant change in the percentage of apoptotic keratocytes compared to controls at any of the used ribo- flavin concentrations （P=0.09 and P=0.13）. We concluded that riboflavin-UVA-photodynamic-inactivation decreases viability of myofibroblastic transformation and multipotent haematopoietic stem cell transformation; however, it does not have an impact on apoptosis of human keratocytes in vitro.

关键词： crosslinking human keratocytes viability apoptosis a-SMA CD34

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Population distribution modelling at fine spatio-temporal scale based on mobile phone data

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International Journal of Digital Earth 2019年第11期12卷 1319-1340页

作者： Petr Kubicek Milan Konecny Zdenek stachon Jie Shen Lukas Herman Tomas Reznik Karel Stanek Radim Stampach Simon Leitgeb Department of Geography Faculty of ScienceMasaryk UniversityBrnoCzech Republic Key Laboratory of Virtual Geographic Environment of Ministry of Education Nanjing Normal UniversityNanjingChina Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application NanjingChina School of Geography Nanjing Normal UniversityNanjingChina

Population distribution modelling can benefit many different domains,for example,transportation,urban planning,ecology or emergency *** about the location and number of people in an affected area is crucial for decision-makers during emergencies and *** phone data represents relatively reliable and time accurate information on real-time population distribution,movement and *** this study,we evaluate the spatio-temporal distribution of population derived from phone data of the selected pilot area(City of Brno,Czech Republic).Analysis is based on the dataset describing the estimated human presence(EHP)with two values-visitors and transiting *** temporal change of data is first analysed and further processed using two methodological ***,the dasymetric method is used where the building geometry and technical attributes served as a target ***,the results of building level analysis are transformed into a regular grid zone of both visitors and the general *** spatio-temporal patterns are compared to the census *** demonstrate how the proposed building level dasymetric approach can improve the spatial granularity of *** use of proposed methodology within selected emergency situations is further discussed.

关键词： Population distribution modelling mobile phone data estimated human presence emergency management

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