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World Journal of Gastroenterology 2014年第42期20卷 15837-15844页

作者： Christoph G Dietrich tanja Kottmann Manuela Alavi Department of Medicine Bethlehem Hospital Teaching Hospital of Aachen University RWTH Private Institute of Medical Statistics

AIM: To investigate the effect of Lactobacillus-containing commercially available probiotic formulations in Germany during antibiotic treatment with an analysis of cost-efficiency.

关键词： Antibiotics-associated diarrhea Probiotics Lactobacillus casei

Heterologous Expression of Mycobacterium tuberculosis Ag85B in Pichia pastoris

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Wuhan University Journal of Natural Sciences 2005年第3期10卷 602-606页

作者： LIUYan PENGJian-hong mali-xin JUWei QUXue-ju LIUJun-yan DepartmentofImmunology SchoolofMedicineWuhanUniversityWuhan430071HubeiChina LaboratoryofMolecularMicrobiology＆GeneEngineering FacultyofLifeScienceHubeiUniversityWuhan430064HubeiChina InstituteofAllergyandImmune-RelatedDisease SchoolofMedicineWuhanUniversityWuhan430071HubeiChina

The DNA fragment encoding mature Mycobacterium tuberculosis major secretory protein Ag85B was inserted into the Pichia pastoris secretory expression vector pHBM905A, under the control of the AOX1 promoter. The recombinant plasmid pHBM905A-85B linearized by Sal I was introduced into Pichia pastoris strain GS115 by PEG1000 transformation method. After phenotype screening and PCR identification, the resulting GS115-pHBM905A-85B strain was cultivated and induced with methanol. The recombinant Ag85B protein in secreted form was attained with molecular weight of 35 × 10^3 approximately detected by SDS-PAGE and Western blot. EI,ISA experiment proved that the protein had good antigen specificity. Secretory expression of recombinant M. tuberculosis Ag85B in P. pastoris will open a door to mass production of the protein in heterologous host and allow ready evaluation of its immunological function.

关键词： Pichia pastoris secretory expression Mycobacterium tuberculosis Ag85B

Hybrid Single Image Super-Resolution Algorithm for Medical Images

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Computers, Materials & Continua 2022年第9期72卷 4879-4896页

作者： Walid El-Shafai Ehab Mahmoud Mohamed Medien Zeghid Anas mali Moustafa H.Aly Department of Electronics and Electrical Communications Engineering Faculty of Electronic EngineeringMenoufia UniversityMenouf32952Egypt Security Engineering Lab Computer Science DepartmentPrince Sultan UniversityRiyadh11586Saudi Arabia Electrical Engineering Department College of EngineeringPrince Sattam Bin Abdulaziz UniversityWadi Addwasir11991Saudi Arabia Electrical Engineering Department Aswan UniversityAswan81542Egypt Electronics and Micro-Electronics Laboratory(E.μ.E.L) Faculty of SciencesUniversity of MonastirMonastir5000Tunisia Alexandria Higher Institute of Engineering&Technology(AIET) AlexandriaEgypt Electronics and Communications Engineering Department College of Engineering and TechnologyArab Academy for ScienceTechnology and Maritime TransportAlexandria1029Egypt

High-quality medical microscopic images used for diseases detection are expensive and difficult to ***,low-resolution images are favorable due to their low storage space and ease of sharing,where the images can be enlarged when needed using Super-Resolution(SR)***,it is important to maintain the shape and size of the medical images while enlarging *** of the problems facing SR is that the performance of medical image diagnosis is very poor due to the deterioration of the reconstructed image ***,this paper suggests a multi-SR and classification framework based on Generative Adversarial Network(GAN)to generate high-resolution images with higher quality and finer details to reduce *** proposed framework comprises five GAN models:Enhanced SR Generative Adversarial Networks(ESRGAN),Enhanced deep SR GAN(EDSRGAN),Sub-Pixel-GAN,SRGAN,and Efficient Wider Activation-B GAN(WDSR-b-GAN).To train the proposed models,we have employed images from the famous BreakHis dataset and enlarged them by 4×and 16×upscale factors with the ground truth of the size of 256×256×***,several evaluation metrics like Peak Signal-to-Noise Ratio(PSNR),Mean Squared Error(MSE),Structural Similarity Index(SSIM),Multiscale Structural Similarity Index(MS-SSIM),and histogram are applied to make comprehensive and objective comparisons to determine the best methods in terms of efficiency,training time,and storage *** obtained results reveal the superiority of the proposed models over traditional and benchmark models in terms of color and texture restoration and detection by achieving an accuracy of 99.7433%.

关键词： GAN medical images SSIM MS-SSIM PSNR SISR

Wood substitution potential in greenhouse gas emission reduction–review on current state and application of displacement factors

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Forest Ecosystems 2021年第3期8卷 548-565页

作者： tanja Myllyviita Sampo Soimakallio Jáchym Judl Jyri Seppälä Finnish Environment Institute Helsinki Finland

Background:Replacing non-renewable materials and energy with wood offers a potential strategy to mitigate climate change if the net emissions of ecosystem and technosystem are reduced in a considered time *** factors(DFs)describe an emission reduction for a wood-based product or fuel which is used in place of a non-wood *** aims of this review were to map and assess DFs from scientific literature and to provide findings on how to harmonise practices behind them and to support coherent ***:Most of the reviewed DFs were positive,implying decreasing fossil GHG emissions in the *** vast majority of the reviewed DFs describe avoided fossil emissions either both in processing and use of wood or only in the latter when wood processing emissions were considered *** of the reviewed DFs included emissions avoided in post-use of harvested wood products(HWPs).Changes in forest and product carbon stocks were not included in DFs except in a few single ***,in most of the reviewed studies they were considered separately in a consistent way along with *** for wood energy,construction and material substitution were widely available,whereas DFs for packaging products,chemicals and textiles were *** than half of DFs were calculated by the authors of the reviewed articles while the rest of them were adopted from other ***:Most of the reviewed DFs describe the avoided fossil GHG *** DFs may provide insights on the wood-based products with a potential to replace emissions intensive alternatives but they do not reveal the actual climate change mitigation effects of wood *** way DFs should be applied and interpreted depends on what has been included in *** the aim of DFs is to describe the overall climate effects of wood use,DFs should include all the relevant GHG flows,including changes in forest and HWP carbon stock and post-use of HWPs,however,based on this lite

关键词： substitution potential replace

Influence of short chain ceramides and lipophilic penetration enhancers on the nano-structure of stratum corneum model membranes studied using neutron diffraction

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Frontiers of Chemical Science and Engineering 2013年第1期7卷 29-36页

作者： Annett SCHROETER tanja ENGELBRECHT Reinhard H. H. NEUBERT Institute of Pharmacy Martin Luther University Halle-Wittenberg D-06120 Halle/Saale Germany

Oriented stratum corneum model lipid membranes were used to study the influence of the short chain ceramides （CER）[NP] and [AP] as well as the impact of the lipophilic penetration enhancer molecules oleic acid （OA） and isopropyl myristate （IPM） on the lipid nanostrueture. The influence of the enhancer molecules were studied using specifically deuterated OA and IPM and neutron diffraction. 2H NMR spectroscopy was used to study the impact of the ceramides＇ degree of order within the stratum corneum model lipid membranes. It was found that CER[NP] forms two very stable phases with high resistance against temperature increase. Phase B showed unusual hydration behavior as no water uptake of this phase was observed. The 2H NMR spectroscopic measurements showed that CER[NP] based ternary model system had a higher state of lamellar order in comparison to CER[AP] based lipid matrix. The studies confirmed that the short chain ceramides, particularly CER[NP], have a very high impact on the integrity of the Stratum comeum lipid bilayers. The penetration enhancer OA has not influenced the repeat distance of the model membrane based on CER[AP], and was not able to induce a phase separation in the investigated lipid matrix. However, a disorder and a fluidisation of the model membranes were observed when OA was incorporated. IPM showed the same effect but two phases （assigned as phase A and B） appeared, when IPM was used as penetration enhancer and incorporated into the model membrane. Furthermore, two arrangements of IPM were identified in phase A using deuterated IPM. A model of the nanostructure of the Stratum corneum lipid membranes is presented.

关键词： nano-structure of the stratum corneum cer-amide penetration enhancer model membrane neutron diffraction 2H NMR spectroscopy

Dual-Targeting of Arabidopsis 6-Phosphogluconolactonase 3 （PGL3）to Chloroplasts and Peroxisomes Involves Interaction with Trx m2 in the Cytosol

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Molecular Plant 2014年第1期7卷 252-255页

作者： Christian Holscher tanja Meyer Antje von Schaewen Institut for Biologie und Biotechnologie der Pflanzen Westfalische Wilhelms-Universitat Munster Schlossgarten 3 48149 Munster Germany

The oxidative pentose-phosphate pathway （OPPP） represents a central branch of cellular metabolism emanating from glucose-6-phosphate （G6P） to provide reductive power （NADPH） and sugar phosphates for anabolic biosyntheses. In plant cells, the oxidative OPPP branch is found in the cytosol and in plastids, consisting of glucose-6-phosphate dehydrogenase （G6PD）, 6-phosphogluconolactonase （PGL）, and 6-phosphogluconate dehydrogenase （6PGD）. These enzymes are encoded by small gene families in the nuclear genome, which, in Arabidopsis, comprise six G6PD, five 6-PGL, and three 6-PGD isoforms （Kruger and von Schaewen, 2003）. Specific targeting motifs at the C-terminus of 6-PGL and 6-PGD isoforms suggested that the OPPP may also occur in peroxisomes （Reumann et al., 2004）.

关键词： Cytosol C-Terminus nuclear genome Plastid Peroxisomes 6-phosphogluconolactonase Gene families Plant cells chloroplasts Glucosephosphate Dehydrogenase Dual Glucose-6-Phosphate Arabidopsis sugar phosphates

Optical vortices 30 years on:OAM manipulation from topological charge to multiple singularities

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Light(Science & Applications) 2019年第1期8卷 339-367页

作者： Yijie Shen Xuejiao Wang Zhenwei Xie Changjun Min Xing Fu Qiang Liu mali Gong Xiaocong Yuan Key Laboratory of Photonic Control Technology(Tsinghua University) Ministry of Education100084 BeijingChina State Key Laboratory of Precision Measurement Technology and Instruments Department of Precision InstrumentTsinghua University100084 BeijingChina National Engineering Laboratory for Public Safety Risk Perception and Control by Big Data(NELPSRPC) China Academy of Electronics and Information Technology of CETCChina Electronic Technology Group Corporation100041 BeijingChina Nanophotonics Research Center Shenzhen University518060 ShenzhenChina

Thirty years ago,Coullet et *** that a special optical field exists in laser cavities bearing some analogy with the superfluid *** then,optical vortices have been widely studied,inspired by the hydrodynamics sharing similar *** to a fluid vortex with a central flow singularity,an optical vortex beam has a phase singularity with a certain topological charge,giving rise to a hollow intensity *** a beam with helical phase fronts and orbital angular momentum reveals a subtle connection between macroscopic physical optics and microscopic quantum *** amazing properties provide a new understanding of a wide range of optical and physical phenomena,including twisting photons,spin–orbital interactions,Bose-Einstein condensates,etc.,while the associated technologies for manipulating optical vortices have become increasingly tunable and ***,owing to these salient properties and optical manipulation technologies,tunable vortex beams have engendered tremendous advanced applications such as optical tweezers,high-order quantum entanglement,and nonlinear *** article reviews the recent progress in tunable vortex technologies along with their advanced applications.

关键词： tunable optics charge

Entropy Generation and Thermal Radiation Effects on Nanofluid over Permeable Stretching Sheet

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Journal of Mathematical Research with Applications 2022年第5期42卷 523-538页

作者： S.K.ASHA G.mali Department of Mathematics Karnatak University

In the present paper, effects of entropy generation and nonlinear thermal radiation on Jeffery nanofluid over permeable stretching sheet with partial slip effect were analyzed. The suitable similarity transformation is utilized for the reduction of a set of governing equations,which are solved by using Differential Transformation Method(DTM) with the help of symbolic software MATHEMATICA. The accuracy of impact of slip parameter on coefficient of skin friction by using DTM and numerical method(Shooting technique with fourth-order RungeKutta) is illustrated and good agreement is found. Further, velocity, temperature, nanoparticle volume fraction and entropy generation profiles are shown graphically and studied in detail for various physical parameters. We notice that, as slip parameter rises the velocity and entropy generation profile rises. Enhancement in the effect of nonlinear thermal radiation reduces the entropy generation.

关键词： stretching sheet partial slip permeable nonlinear thermal radiation entropy generation Jeffrery nanofluid

Cortisol levels in skimmed milk during the first 22 weeks of lactation and response to short-term metabolic stress and lameness in dairy cows

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Journal of Animal Science and Biotechnology 2015年第4期6卷 485-491页

作者： Katharina Gellrich tanja Sigl Heinrich H.D.Meyer Steffi Wiedemann Physiology Weihenstephan Technische Universitaet Muenchen Animal Health Institute of Animal Breeding and HusbandryKiel University

Background： Cortisol is secreted into blood in reaction to acute stress, but also in phases of diminished feed intake and changed animal behavior. As cows do not always show clear signs of discomfort, reliable diagnostic markers could be used to provide information regarding individual cows＇ distress. The objective of this study was to establish an ether free immunoassay for the detection of cortisol and to determine values during the first 22 weeks of lactation. Furthermore, the response in milk cortisol levels was assessed during times of metabolic stress and pain associated symptoms of lameness. Methods： Milk yield and composition, blood serum glucose, NEFA and BHBA as well as milk cortisol were determined in 24 multiparous Holstein-Friesian cows over the course of the first 22 weeks of lactation. Animals were further checked for signs of clinical diseases on a daily basis. Two feed restrictions over three days （FR; 70 % of precious ad libitum intake） were performed during the 4th wk and the 21st wk, respectively. An ELISA for cortisol measurement in easily accessible bovine skimmed milk was established and applied. Results： On the last day of FR in early lactation, a reduction in milk yield and changes in serum metabolites compared to respective previous values were detected. The FR in mid-lactation resulted in no changes in milk production and serum metabolites. Milk cortisol was highest during first wk of lactation and remained on comparable levels thereafter. Milk yield and composition were not influenced by FR. Lameness resulted in enhanced milk cortisol levels. Conclusion： Milk cortisol could be used as an indicator of painful symptoms such as lameness. Higher values of milk cortisol levels during first wk of lactation should be taken into account for interpretation.

关键词： Cortisol Dairy cow ELISA Feed restriction Milk

Silica chloride catalyzed one-pot synthesis of fully substituted pyrazoles

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Chinese Chemical Letters 2011年第10期22卷 1187-1190页

作者： Dhanaji V.Jawale Umesh R.Pratap Jyotirling R.mali Ramrao A.Mane Department of Chemistry Dr.Babasaheb Ambedkar Marathwada UniversityAurangabad(M.S.) 431 004India

Silica chloride catalysted one pot cyclocondensation of aldehydes,ethyl acetoacetate and phenyl hydrazine leading to substituted pyrazoles has been reported.

关键词： One-pot synthesis Silica chloride Solvent free Fully substituted pyrazoles