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FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury

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Military Medical Research 2024年第4期11卷 521-542页

作者： Mi Zhou Yang-Wu-Yue Liu Yu-Hang He Jing-Yu Zhang Hao Guo Hao Wang Jia-Kui Ren Yi-Xun Su Teng Yang Jia-Bo Li Wen-Hui He Peng-Jiao Ma man-Tian Mi Shuang-Shuang Dai Department of Biochemistry and Molecular Biology School of Basic MedicineArmy Medical UniversityChongqing 400038China Research Center for Nutrition and Food Safety Chongqing Key Laboratory of Nutrition and HealthInstitute of Military Preventive MedicineArmy Medical UniversityChongqing 400038China Department of Neurosurgery Daping HospitalArmy Medical UniversityChongqing 400042China Department of Trauma and Emergency Southwest HospitalArmy Medical UniversityChongqing 400038China Department of Histology and Embryology Chongqing Key Laboratory of NeurobiologyBrain and Intelligence Research Key Suyixun Laboratory of Chongqing Education CommissionArmy Medical UniversityChongqing 400038China Research Center Seventh Affiliated Hospital of Sun Yat-Sen UniversityShenzhen 518107GuangdongChina

Background:Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury(TBI).However,the heterogeneity,multifunctionality,and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly ***:Using the combined single-cell transcriptomics,metabolomics,and proteomics analysis from TBI patients and the TBI mouse model,we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral *** also characterized the underlying mechanisms both invitro and invivo through molecular simulations,signaling detections,gene expression regulation assessments[including dual-luciferase reporter and chromatin immunoprecipitation(ChIP)assays],primary cultures or co-cultures of neutrophils and oligodendrocytes,intracellular iron,and lipid hydroperoxide concentration measurements,as well as forkhead box protein O1(FOXO1)conditional knockout ***:We identified that high expression of the FOXO1 protein was induced in neutrophils after TBI both in TBI patients and the TBI mouse *** of these FOXO1high neutrophils in the brain was detected not only in the acute phase but also in the chronic phase post-TBI,aggravating acute brain inflammatory damage and promoting late TBI-induced *** the acute stage,FOXO1 upregulated cytoplasmic Versican(VCAN)to interact with the apoptosis regulator B-cell lymphoma-2(BCL-2)-associated X protein(BAX),suppressing the mitochondrial translocation of BAX,which mediated the antiapoptotic effect companied with enhancing interleukin-6(IL-6)production of FOXO1high *** the chronic stage,the“FOXO1-transferrin receptor(TFRC)”mechanism contributes to FOXO1high neutrophil ferroptosis,disturbing the iron homeostasis of oligodendrocytes and inducing a reduction in myelin basic protein,which contributes to the progression of late depression afte

关键词： Traumatic brain injury(TBI) Neutrophil Forkhead box protein O1(FOXO1) Acute stage Chronic stage

Shuxuening Injection Inhibits Apoptosis and Reduces Myocardial Ischemia-Reperfusion Injury in Rats through PI3K/AKT Pathway

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Chinese Journal of Integrative Medicine 2024年第5期30卷 421-432页

作者： YUE Tong-tong CAO Ying-jie CAO Ya-xuan LI Wei-xia WANG Xiao-yan SI Chun-ying XIA Han ZHU Ming-jun tang Jin-fa WANG He The First Clinical Medical College of Henan University of Chinese Medicine Zhengzhou 450046China Department of Pharmacy the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China Department of Cardiovascular Diseases the First Affiliated Hospital of Henan University of Chinese MedicineZhengzhou 451200China

Objective: To investigate the main components and potential mechanism of Shuxuening Injection(SXNI) in the treatment of myocardial ischemia-reperfusion injury(MIRI) through network pharmacology and in vivo research. Methods: The Traditional Chinese Medicine Systems Pharmacology(TCMSP) and Pharm Mapper databases were used to extract and evaluate the effective components of Ginkgo biloba leaves, the main component of SXNI. The Online Mendelian Inheritance in man(OMIM) and Gene Cards databases were searched for disease targets and obtain the drug target and disease target intersections. The active ingredient-target network was built using Cytoscape 3.9.1 software. The STRING database, Metascape online platform, and R language were used to obtain the key targets and signaling pathways of the anti-MIRI effects of SXNI. In order to verify the therapeutic effect of different concentrations of SXNI on MIRI in rats, 60 rats were first divided into 5 groups according to random number table method: the sham operation group, the model group, SXNI low-dose(3.68 mg/kg), medium-dose(7.35 mg/kg), and high-dose(14.7 mg/kg) groups, with 12 rats in each group. Then, another 60 rats were randomly divided into 5 groups: the sham operation group, the model group, SXNI group(14.7 mg/kg), SXNI+LY294002 group,and LY294002 group, with 12 rats in each group. The drug was then administered intraperitoneally at body weight for 14 days. The main biological processes were validated using in vivo testing. Evans blue/triphenyltetrazolium chloride(TTC) double staining, hematoxylin-eosin(HE) staining, terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) assay, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis were used to investigate the efficacy and mechanism of SXNI in MIRI rats. Results: Eleven core targets and 30 Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways were selected. Among these, the phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT) pathway was

关键词： myocardial ischemia reperfusion injury PI3K/AKT signaling pathway Shuxuening Injection apoptosis Ginkgo biloba leaves

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铜仁农村人居环境质量评价及空间分布研究

城市建筑 2024年第9期21卷 117-119,126页

作者：石勇美唐漫叶强铜仁职业技术学院铜仁市建筑勘察设计院有限公司

农村人居环境是一个综合复杂的动态的巨系统,是实施乡村振兴战略的重点任务。文章以铜仁市10个县(区)为研究对象,以生产条件、生活质量和生态环境为系统层,粮食生产率、农业机械总动力等20个指标构建铜仁市农村人居环境质量评价指标体系... 详细信息

农村人居环境是一个综合复杂的动态的巨系统,是实施乡村振兴战略的重点任务。文章以铜仁市10个县(区)为研究对象,以生产条件、生活质量和生态环境为系统层,粮食生产率、农业机械总动力等20个指标构建铜仁市农村人居环境质量评价指标体系,运用熵值法确定其指标权重,以ArcGIS软件直观表示其空间分布情况。结果表明,铜仁市农村人居环境质量综合指数整体较低,空间分布不均衡,整体呈东高西低空间分布格局;各子系统质量差异显著,生态环境呈现西高东低格局,生产和生活系统空间整体上与农村人居环境质量综合指数基本一致。

关键词：农村人居环境铜仁熵值法

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基于工匠精神塑造的“食品工程原理”课程思政沁润路径

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食品工业 2024年第5期45卷 233-235页

作者：唐文婷李曼蒲传奋青岛农业大学食品科学与工程学院青岛266109

在树立和践行“大食物观”的时代背景下,为塑造学生作为未来食品人的工匠精神,系统分析“食品工程原理”课程思政教学实施的必要性和价值意蕴。从重构“食品工程原理”课程思政育人目标、加强课程思政教学团队建设、构建联动育人机制、... 详细信息

在树立和践行“大食物观”的时代背景下,为塑造学生作为未来食品人的工匠精神,系统分析“食品工程原理”课程思政教学实施的必要性和价值意蕴。从重构“食品工程原理”课程思政育人目标、加强课程思政教学团队建设、构建联动育人机制、融入课程相关思政元素、建立多元动态思政育人教学评估体系角度出发,探索新形势下“食品工程原理”课程思政实施路径。课程思政建设重点塑造学生工匠精神,同时培养学生家国情怀、理想信念、创新精神和哲学思辨能力,以期为食品科学专业工程类课程思政建设和高素质创新型人才培养提供借鉴。

关键词：课程思政工匠精神食品工程原理

低碳视角下北京西四北二条3号院更新改造与能耗模拟研究

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建筑节能（中英文） 2024年第1期52卷 109-113,148页

作者：张曼曹雪萦汤羽扬北京建筑大学建筑与城市规划学院北京100044 北京建筑大学建筑遗产研究院北京100044

通过实地调研北京老城西四北头条至八条历史文化街区的四合院传统民居,并与居民开展改造诉求访谈,在提升人居环境的基础上探索合适的改造策略,借助EnergyPlus能耗模拟研究改造前后能耗变化,达到老城民居低碳节能改造的目的,并分析其在... 详细信息

通过实地调研北京老城西四北头条至八条历史文化街区的四合院传统民居,并与居民开展改造诉求访谈,在提升人居环境的基础上探索合适的改造策略,借助EnergyPlus能耗模拟研究改造前后能耗变化,达到老城民居低碳节能改造的目的,并分析其在探索中国典型四合院民居的能源性能改善方法的意义。研究结果表明,改造后的民居全年耗能18960.21 kW·h,平均每平方米供暖能耗为19.64 kW·h/(m^(2)·a)。改造后的各房屋围护结构系数均发生变化,如建筑外墙传热系数优化为0.259 W/(m^(2)·K),屋顶传热系数优化为0.181 W/(m^(2)·K),楼板传热系数优化为0.131 W/(m^(2)·K),外窗传热系数优化为1.1 W/(m^(2)·K),符合北京当前对于住宅节能的相关规范。如果一套保温性能更好的四合院传统民居能够在城市更新进程中作为一个组成部分传承下来,将有助于中国老城历史片区传统风貌保护与传统建筑低碳节能优化。

关键词：城市更新北京老城四合院能源性能 EnergyPlus

Cu(Ⅱ)结合对3种黄酮醇分子结构与抗氧化活性影响的理论分析

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林产化学与工业 2024年第4期44卷 130-138页

作者：管勤昊汤丽华徐曼张亮亮黄立新中国林业科学研究院林产化学工业研究所、江苏省生物质能源与材料重点实验室、国家林业和草原局林产化学工程重点实验室、林木生物质低碳高效利用国家工程研究中心、江苏省林业资源高效加工利用协同创新中心江苏南京210042 华侨大学先进碳转化技术研究院福建厦门361021

为揭示黄酮醇与Cu(Ⅱ)之间的相互作用机制,进而提升黄酮醇-Cu(Ⅱ)配位化合物的抗氧化活性,采用密度泛函理论B3LYP方法,分析了羟基去质子化及结合Cu(Ⅱ)对水溶液中3种林源膳食黄酮醇山奈酚、槲皮素、杨梅素分子结构与抗氧化活性的影响。... 详细信息

为揭示黄酮醇与Cu(Ⅱ)之间的相互作用机制,进而提升黄酮醇-Cu(Ⅱ)配位化合物的抗氧化活性,采用密度泛函理论B3LYP方法,分析了羟基去质子化及结合Cu(Ⅱ)对水溶液中3种林源膳食黄酮醇山奈酚、槲皮素、杨梅素分子结构与抗氧化活性的影响。结果表明:羟基去质子化后,黄酮醇分子内其他O—H键键长减小、苯环结构二面角发生改变、分子偶极矩增加、最高占有分子轨道(HOMO)与最低未占有分子轨道(LUMO)能级差(ΔE)减小、分子内其他羟基氢原子正电荷数减小;结合Cu(Ⅱ)后,黄酮醇分子内其他O—H键键长增加、二面角与偶极矩改变、ΔE减小、氢原子正电荷数增大。研究还发现:去质子化后的黄酮醇主要通过改变分子结构和减小ΔE提升离子活性,于去质子化位点形成较高负电势,从而通过静电相互作用与Cu(Ⅱ)发生配位;结合Cu(Ⅱ)后的黄酮醇主要通过增加键长、改变分子结构、增加氢原子正电荷数促进与自由基结合,减小ΔE提升分子活性,增强其抗氧化能力。

关键词：密度泛函理论林源膳食黄酮醇分子结构抗氧化

融媒体时代高校思想政治教育叙事话语体系建设探析

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兰州文理学院学报（社会科学版） 2024年第1期40卷 83-88页

作者：杨筱曼汤先萍刘剑新疆医科大学马克思主义学院新疆乌鲁木齐830011

思想政治教育叙事话语是中国话语的重要组成部分,是传播中国声音的主要方式。建设符合融媒体时代特征的思想政治教育叙事话语体系,有利于培养大学生政治素养、提升大学生思想道德素质和传承中华优秀传统文化。思想政治教育叙事话语面临... 详细信息

思想政治教育叙事话语是中国话语的重要组成部分,是传播中国声音的主要方式。建设符合融媒体时代特征的思想政治教育叙事话语体系,有利于培养大学生政治素养、提升大学生思想道德素质和传承中华优秀传统文化。思想政治教育叙事话语面临政治引导与多元需求的冲突、内容构建与形式表达的冲突和真理唯一与价值多元的冲突。重构叙事话语内容、优化叙事话语场域和创新叙事话语方式是构建思想政治教育叙事话语体系的有效路径。

关键词：思想政治教育叙事话语融媒体

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The burgeoning importance of PIWI-interacting RNAs in cancer progression

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Science China(Life Sciences) 2024年第4期67卷 653-662页

作者： Xinpei Deng Tianle Liao Jindong Xie Da Kang Yiwei He Yuying Sun Zhangling Wang Yongluo Jiang Xuan Miao Yixuan Yan Hailin tang Lewei Zhu Yutian Zou Peng Liu State Key Laboratory of Oncology in South China Guangdong Provincial Clinical Research Center for CancerSun Yat-sen University Cancer CenterGuangzhou 510060China School of Medicine Sun Yat-sen UniversityShenzhen 518107China Key Laboratory of Cardiovascular and Cerebrovascular Medicine Nanjing Medical UniversityNanjing 211166China Guanghua School of Stomatology Sun Yat-sen UniversityGuangzhou 510062China The First People’s Hospital of Foshan Foshan 528000China

PIWI-interacting RNAs(pi RNAs)are a class of small noncoding RNA molecules that specifically bind to piwi protein family members to exert regulatory functions in germ *** studies have found that pi RNAs,as tissue-specific molecules,both play oncogenic and tumor suppressive roles in cancer progression,including cancer cell proliferation,metastasis,chemoresistance and ***,the atypical manifestation of pi RNAs and PIWI proteins in various malignancies presents a promising strategy for the identification of novel biomarkers and therapeutic targets in the diagnosis and management of ***,the precise functions of pi RNAs in cancer progression and their underlying mechanisms have yet to be fully *** review aims to examine current research on the biogenesis and functions of pi RNA and its burgeoning importance in cancer progression,thereby offering novel perspectives on the potential utilization of pi RNAs and piwi proteins in the management and treatment of advanced cancer.

关键词： PIWI-interacting RNAs/piRNAs cancer progression metastasis chemoresistance stemness

Ultrasomics in liver cancer: Developing a radiomics model for differentiating intrahepatic cholangiocarcinoma from hepatocellular carcinoma using contrast-enhanced ultrasound

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World Journal of Radiology 2024年第7期16卷 247-255页

作者： Li-Ya Su Ming Xu Yan-Lin Chen man-Xia Lin Xiao-Yan Xie Department of Medical Ultrasound The First Affiliated HospitalInstitute of Diagnostic and Interventional UltrasoundSun Yat-Sen UniversityGuangzhou 510000Guangdong ProvinceChina

BACKGROUND Hepatocellular carcinoma(HCC)and intrahepatic cholangiocarcinoma(ICC)represent the predominant histological types of primary liver cancer,comprising over 99%of *** their differing biological behaviors,prognoses,and treatment strategies,accurately differentiating between HCC and ICC is crucial for effective clinical ***,an emerging image processing technology,can automatically extract various quantitative image features that may elude the human *** on the application of ultrasound(US)-based radiomics methods in distinguishing HCC from ICC are *** In our retrospective study,we included a total of 280 patients who were diagnosed with ICC(n=140)and HCC(n=140)between 1999 and *** patients were divided into training(n=224)and testing(n=56)groups for *** images and relevant clinical characteristics were *** utilized the XGBoost method to extract and select radiomics features and further employed a random forest algorithm to establish ultrasomics *** compared the diagnostic performances of these ultrasomics models with that of *** Four distinct ultrasomics models were constructed,with the number of selected features varying between models:13 features for the US model;15 for the contrast-enhanced ultrasound(CEUS)model;13 for the combined US+CEUS model;and 21 for the US+CEUS+clinical data *** US+CEUS+clinical data model yielded the highest area under the receiver operating characteristic curve(AUC)among all models,achieving an AUC of 0.973 in the validation cohort and 0.971 in the test *** performance exceeded even the most experienced radiologist(AUC=0.964).The AUC for the US+CEUS model(training cohort AUC=0.964,test cohort AUC=0.955)was significantly higher than that of the US model alone(training cohort AUC=0.822,test cohort AUC=0.816).This finding underscored the significant benefit of incorporating CEUS information in accurately distin-guishing ICC from HC

关键词： Cholangiocarcinoma Hepatocellular carcinoma Contrast-enhanced ultrasound Radiomics Primary liver tumor