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江苏省生物医学实验室科研人员暴露调查分析

南京医科大学学报（自然科学版） 2024年第1期44卷 65-71页

作者：张燕黄剑刘红唐梅张芙蓉吴菁沈宁薛斌南京医科大学附属逸夫医院医学工程部江苏南京211112 中国药科大学生命科学与技术学院江苏南京211198 南京医科大学附属逸夫医院中心实验室江苏南京211112 瑞测精准医学检测(上海)有限公司上海200120 南京医科大学附属逸夫医院检验科江苏南京211112

目的:检测江苏省生物医学实验室科研人员体内环境污染物残留状况,为实验室生物安全制度的制定提供依据。方法:在江苏省南京、苏州、盐城、淮安4个地区高校内分别选取100例生物医学实验室科研人员和100例非科研人员为研究对象,采用问卷... 详细信息

目的:检测江苏省生物医学实验室科研人员体内环境污染物残留状况,为实验室生物安全制度的制定提供依据。方法:在江苏省南京、苏州、盐城、淮安4个地区高校内分别选取100例生物医学实验室科研人员和100例非科研人员为研究对象,采用问卷调查了解不同人群接触环境污染物的现状,并进一步收集研究对象的晨尿样本,其中科研人员和非科研人员分别收集到了95例和94例样本。采用β-葡萄糖醛酸苷酶水解法和高效液相色谱-串联质谱法检测尿液中邻苯二甲酸盐[di(2-ethylhexyl)phthalate,DEHP]、双酚A和苯并芘的含量。结果:江苏省内生物医学实验室科研人员尿液中DEHP、双酚A、苯并芘的残留量[(1359.0±251.0)μg/g、(284.4±134.0)μg/g、(109.0±43.2)ng/g)]显著高于对照组人员[(489.7±103.1)μg/g、(177.3±86.4)μg/g、(80.2±31.5)ng/g,Png/g、(139.3±32.8)ng/g],DEHP在各地区实验室人员体内没有明显差异,而盐城地区实验室人员体内的双酚A含量最高[(386.1±202.1)μg/g]。结论:江苏省各地区生物医学实验室科研人员体内积累的环境污染物普遍高于非实验室人员,这可能与日常实验室工作相关,因此实验室科研人员更应注重防护。

关键词：生物医学实验室科研人员环境污染物尿液

Interleukin-4 affects microglial autophagic flux

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Neural Regeneration Research 2019年第9期14卷 1594-1602页

作者： Run-Hong tang Rui-Qun Qi Hua-Yan Liu Department of Neurology the First Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Department of Dermatology Key Laboratory of Immunodermatologythe First Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Interleukin-4 plays an important protective role in Alzheimer’s disease by regulating microglial phenotype,phagocytosis of amyloid-β,and secretion of anti-inflammatory and neurotrophic ***,increasing evidence has suggested that autophagy regulates innate immunity by affecting M1/M2 polarization of microglia/***,the role of interleukin-4 in microglial autophagy is *** view of this,BV2 microglia were treated with 0,10,20 or 50 ng/mL interleukin-4 for 24,48,or 72 ***,light chain 3-II and p62 protein expression levels were detected by western blot ***2 microglia were incubated with interleukin-4(20 ng/mL,experimental group),3-methyladenine(500μM,autophagy inhibitor,negative control group),rapamycin(100 nM,autophagy inductor,positive control group),3-methyladenine+interleukin-4(rescue group),or without treatment for 24 hours,and then exposed to amyloid-β(1μM,model group)or vehicle control(control)for 24 ***3-II and p62 protein expression levels were again detected by western blot *** addition,expression levels of multiple markers of M1 and M2 phenotype were assessed by real-time fluorescence quantitative polymerase chain reaction,while intracellular and supernatant amyloid-βprotein levels were measured by enzyme-linked immunosorbent *** results showed that interleukin-4 induced microglial autophagic flux,most significantly at 20 ng/mL for 48 ***-4 pretreated microglia inhibited blockade of amyloid-β-induced autophagic flux,and promoted amyloid-βuptake and degradation partly through autophagic flux,but inhibited switching of amyloid-β-induced M1 phenotype independent on autophagic *** results indicate that interleukin-4 pretreated microglia increases uptake and degradation of amyloid-βin a process partly mediated by autophagy,which may play a protective role against Alzheimer’s disease.

关键词： nerve regeneration Alzheimer’s disease interleukin-4 amyloid-β microglial autophagy microglial polarization microglia M1 phenotype M2 phenotype peptide degradation neural regeneration

苦参碱通过Hippo信号通路促进大鼠皮肤创面愈合及血管生成

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医学研究与战创伤救治 2024年第1期37卷 23-31页

作者：曾华郑勇周林冯上新唐俊孙妍婧罗玮武汉市中医医院肛肠科武汉430014 武汉市中医医院病理科武汉430014 湖北省第三人民医院全科医学科武汉430030

目的探讨苦参碱(MT)对皮肤创面愈合的影响及可能机制。方法在48只大鼠中成功建立全层皮肤切除创伤模型,将模型大鼠随机分为阴性对照组(模型组,涂抹100μL等渗盐水)、阳性药物组(PD组,涂抹100μL的京万红软膏)、苦参碱组(MT组,涂抹浓度为... 详细信息

目的探讨苦参碱(MT)对皮肤创面愈合的影响及可能机制。方法在48只大鼠中成功建立全层皮肤切除创伤模型,将模型大鼠随机分为阴性对照组(模型组,涂抹100μL等渗盐水)、阳性药物组(PD组,涂抹100μL的京万红软膏)、苦参碱组(MT组,涂抹浓度为200 ng/mL的苦参碱混合液100μL)、苦参碱+维替泊芬组(MT+VP组,涂抹苦参碱混合液100μL后,腹腔注射100 mg/kg维替泊芬),每组12只。各组每日给药1次,持续7 d。比较各组大鼠的创面愈合率、病理组织损伤情况、CD31阳性染色细胞比例,以及血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平。蛋白免疫印迹检测(Western blot)Hippo通路相关蛋白表达。结果造模后3、7、14 d时,与模型组创面愈合率[(14.02±1.87)%、(36.23±3.62)%、(65.53±3.85)%]相比,PD组[(21.36±2.51)%、(61.86±3.13)%、(94.26±0.26)%]和MT组[(21.57±2.24)%、(54.35±3.66)%、(94.18±3.13)%]均明显升高(P<0.05);与MT组相比,MT+VP组创面愈合率[(18.35±2.03)%、(43.68±3.54)%、(74.26±3.55)%]均明显降低(P<0.05)。造模后7 d,与模型组(186.75±18.53、85.73±10.15、57.96±5.85)相比,PD组(89.75±9.75、43.12±5.75、8.76±1.13)和MT组(92.57±23.62、46.62±6.54、12.36±1.06)的IL-1β、IL-6、TNF-α水平均明显降低(P<0.05),CD31阳性染色细胞百分比明显升高(P<0.05),MST1、LAST1和YAP蛋白磷酸化水平明显升高(P<0.05),而YAP核移位明显降低(P<0.05)。与MT组相比,MT+VP组IL-1β、IL-6、TNF-α水平均明显升高,CD31阳性染色细胞百分比明显降低,MST1、LAST1和YAP蛋白磷酸化水平明显降低,而YAP核移位明显增加(P<0.05)。与模型组皮肤创面完全愈合时间[(26.33±0.94)d]和瘢痕厚度[(0.58±0.11)mm]相比,PD组[(19.50±0.82)d、(0.24±0.04)mm]和MT组[(19.00±0.50)d、(0.25±0.04)mm]明显减少(P<0.05);与MT组相比,MT+VP组创面完全愈合时间[(22.33±0.94)d]和瘢痕厚度[(0.41±0.04)mm]均明显增加(P<0.05)。结论苦参碱能够通过抑制炎症反应、促进血管生成加速全层皮肤切除创伤大鼠的皮肤创面愈合,这一过程可能涉及苦参碱对Hippo-Yap通路的激活效应。

关键词：皮肤创面愈合苦参碱炎症血管生成 Hippo通路

Activation of c-Jun N-terminal Kinases by Ribotoxic Stresses

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Cellular & Molecular Immunology 2005年第6期2卷 419-425页

作者： Dong-Yun Ouyang Yuan-Yuan Wang Yong-tang Zheng Key Laboratory of Animal Models and Human Diseases Mechanisms and Laboratory of Molecular Immunopharmacology Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China

The c-Jun N-terminal kinases （JNKs） are classic stress-activated protein kinases. Many cellular stresses have been shown to stimulate JNK activation. In this review, we focus on ribotoxic stresses based on their multiple biological potencies including anti-HIV-1 activity. Some of the functions of ribotoxins and the signaling transduction pathway that mediated are mentioned. Different from other stimulators, ribotoxic stresses act on special motifs of 28S rRNA in translationally active mammal ribosomes. Binding and damaging on the motif leads to JNK activation and subsequently biological response to the signal initiator, which is named ribotoxic stress response. Cellular ＆ Molecular Immunology. 2005;2（6）：419-425.

关键词： ribotoxic stress response JNK MAPK apoptosis ribotoxin ribosome inactivating protein HIV

中国西北地区天主教及基督新教史研究现状与史料

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西北民族研究 2011年第4期 103-121页

作者：汤开建曾金莲澳门大学历史系

研究西北地区的基督教史,具有重大意义。因为基督教在西北地区的历史源远流长,可上溯至唐元。该地区交通不便,环境相对恶劣,而且信仰状况复杂,民族语言多样。基督教在西北地区的活动,充分体现了中西交流的丰富历史图景。不过,目前西北... 详细信息

研究西北地区的基督教史,具有重大意义。因为基督教在西北地区的历史源远流长,可上溯至唐元。该地区交通不便,环境相对恶劣,而且信仰状况复杂,民族语言多样。基督教在西北地区的活动,充分体现了中西交流的丰富历史图景。不过,目前西北地区基督教研究仍显得不足,主要表现在研究队伍薄弱,对中西文史料缺乏深入挖掘上。鉴于此,本文着意梳理20世纪80年代以来西北地区基督教史研究状况(不涉及唐元两代的景教研究),整理西北地区基督教史档案史料的分布情况,并对西北地区基督教史的未来走向提出一些粗浅看法。

关键词：西北地区天主教基督新教史料

Metal-to-ligand charge transfer chirality-based sensing of mercury ions

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Photonics Research 2021年第2期9卷 213-221页

作者： XIOngBIN WAng QIUSHI WAng YULOng CHEN JIAGEN LI RUIKUN PAN XIng CHEng KAR WEI ng XI ZHU TIngCHAO HE JIAJI CHEng ZIKAng tang RUI CHEN Institute of Applied Physics and Materials Engineering University of MacaoAvenida da UniversidadeTaipaMacao 999078China Department of Electrical and Electronic Engineering Southern University of Science and TechnologyShenzhen 518055China School of Materials Science and Engineering Hubei UniversityWuhan 430062China Department of Materials Science and Engineering Southern University of Science and TechnologyShenzhen 518055China Shenzhen Institute of Artificial Intelligence and Robotics for Society(AIRS) Shenzhen 518172China College of Physics and Optoelectronic Engineering Shenzhen UniversityShenzhen 518060China

Chiral ligand conjugated transition metal oxide nanoparticles(NPs) are a promising platform for chiral recognition, biochemical sensing, and chiroptics. Herein, we present chirality-based strategy for effective sensing of mercury ions via ligand-induced chirality derived from metal-to-ligand charge transfer(MLCT) effects. The ligand competition effect between molybdenum and heavy metal ions such as mercury is designated to be essential for MLCT chirality. With this know-how, mercury ions, which have a larger stability constant(Kf) than molybdenum, can be selectively identified and quantified with a limit of detection(LOD) of 0.08 and 0.12 nmol/L for D-cysteine and L-cysteine(Cys) capped Mo O2 NPs. Such chiral chemical sensing nanosystems would be an ideal prototype for biochemical sensing with a significant impact on the field of biosensing, biological systems,and water research-based nanotoxicology.

关键词： mercury molybdenum charge

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水稻群体分蘖动态模型构建与应用

农业工程学报 2024年第10期40卷 213-221页

作者：王小卉李绪孟唐启源邹丹罗友谊李可夫彭剑李灿曹乐平湖南农业大学信息与智能科学技术学院长沙410128 湖南农业大学农学院长沙410128 衡阳市农业科学院衡阳421200 衡山县农业农村局衡阳421399 同济大学国豪书院上海200092 宁远县农业农村局宁远425600 湖南生物机电职业技术学院长沙410127

为定量分析水稻群体茎蘖数量动态变化过程及分蘖动态特征,该研究使用双Logistic模型分别描述分蘖发生与死亡过程,建立水稻群体分蘖动态模型;根据水稻分蘖过程的时序特征定义描述分蘖过程的特征指标,并推导出分蘖特征指标的计算式;基于... 详细信息

为定量分析水稻群体茎蘖数量动态变化过程及分蘖动态特征,该研究使用双Logistic模型分别描述分蘖发生与死亡过程,建立水稻群体分蘖动态模型;根据水稻分蘖过程的时序特征定义描述分蘖过程的特征指标,并推导出分蘖特征指标的计算式;基于不同基因型品种的种植方式、种植时期、种植密度下水稻分蘖动态数据集检验模型优度和适应性;并应用分蘖动态模型和指标探索分蘖动态对种植密度的响应规律。结果表明,所建模型对不同基因型水稻品种在不同种植方式、种植时期和种植密度下的分蘖动态数据拟合优度较好,标准均方根误差S_(RMSE)服从均值小于5%的Gamma分布,并且99%的S_(RMSE)小于10%。基于所建模型计算的分蘖特征指标(包括模型参数)对种植密度有很好的响应;留一法检验表明模型的预测性较好,观测值与模拟值的R^(2)=0.96。所建模型能够精确描述水稻茎蘖数量演变过程,具有很好的拟合优度、适应性和可解释性,可用于分析基因、环境、农艺措施对分蘖动态的影响,分蘖特征指标可望成为分析基因与环境互作的重要表型参数,对指导水稻精准栽培也有重要理论价值和实际意义。

关键词：水稻模型分蘖动态双Logistic模型种植密度参数拟合

TaVNS reduces inflammatory responses in a L-NAME-induced rat model of pre-eclampsia

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BIOCELL 2021年第5期45卷 1231-1240页

作者： LINMEI ZHEng ROng tang LEI SHI ZHOngYI ZHOU Department of Obstetrics Hainan General HospitalHainan Affiliated Hospital of Hainan Medical UniversityHaikou570100China Department of General Surgery Hainan General HospitalHainan Affiliated Hospital of Hainan Medical UniversityHaikou570100China Department of ICU Hainan General HospitalHainan Affiliated Hospital of Hainan Medical UniversityHaikou570100China

Pre-eclampsia is characterized by an excessive maternal inflammatory *** cholinergic antiinflammatory pathway(CAP)has been shown as the efferent arm of a vagal reflex with the potential to limit inflammatory ***,in this study,the CAP regulation through the nervous vagal stimulation(VNS)reduced the severity of ng-nitro-L-arginine methyl ester(L-NAME)-induced pre-eclampsia was determined in a rat *** were given 125 mg/kg/day of L-NAME via subcutaneous injection on gestational day(GD)10–*** addition,the rats were treated by active or sham electrical stimulation once a day during GD 13–*** blood pressure(SBP),urinary albumin,and pregnancy outcomes were documented for each *** average fetal weights and crown-rump length(CRL)as well as the placental weights of rats in both control and experimental groups were recorded onthe 13th day,16th day and 20th day of ***,placentas were collected from the rats on GD20 to measure the level of *** addition,qRT-PCR and Western blot analysis were used to detect the mRNA and protein expression ofα7 nicotinic acetylcholine receptor(α7nAChR)and nuclear factor-κB(NF-κB),*** assays were also carried out to determine the location and level ofα7nAChR and NF-κB in *** regulation through the transcutaneous auricular nerve stimulation alleviated the clinical symptoms in the rats after L-NAME induction,including hypertension,proteinuria,fetal growth retardation and fetal *** addition,TaVNS also increasedα7nAChR expression,reduced NF-κB p65 expression,and reversed LNAME-induced proinflammatory cytokines in the placenta tissues,including tumor necrosis factor-alpha(TNF-α),high mobility group box 1(HMGB-1)and interleukin-6(IL-6).The findings of this study showed that TaVNS might be used as a promising tool to attenuate pre-eclampsia-like *** addition,the protective effect of TaVNS was associated with the improvement ofα7nAChR

关键词： Pre-eclampsia Cholinergic anti-inflammatory pathway TaVNS Inflammation

UPLC-MS/MS法测定人血浆中的阿哌沙班

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华西药学杂志 2024年第4期39卷 411-415页

作者：唐维英杨姗姗魏伯平吴强袁军朱恒怡四川省药品检验研究院国家药监局药物制剂体内外相关性技术研究重点实验室四川成都611731

目的采用超高效液相色谱-串联质谱法测定人血浆中的阿哌沙班。方法采用乙腈沉淀蛋白预处理血浆样品,WatersXbridgeC_(18)色谱柱(50mm×2.1mm,3.5μm)进行分离,以5%乙腈(A)和95%乙腈(B)的含0.1%甲酸水溶液作为流动相,梯度洗脱,流速0.4mL... 详细信息

目的采用超高效液相色谱-串联质谱法测定人血浆中的阿哌沙班。方法采用乙腈沉淀蛋白预处理血浆样品,WatersXbridgeC_(18)色谱柱(50mm×2.1mm,3.5μm)进行分离,以5%乙腈(A)和95%乙腈(B)的含0.1%甲酸水溶液作为流动相,梯度洗脱,流速0.4mL·min^(-1),柱温40℃,进样量2μL。采用电喷雾离子源,正离子多反应监测模式扫描,用于定量分析的离子对分别为m/z460.3→443.3(阿哌沙班)、m/z464.4→447.3(^(13)C阿哌沙班-d_(3)内标)。结果0.5~400ng·mL^(-1)阿哌沙班与峰面积的线性关系良好(r=0.9995),定量下限为0.5ng·mL^(-1),提取回收率≥99.22%,质控样品的批内、批间RSD均≤4.53%;全血样品和血浆样品的稳定性符合生物样品分析的要求。结论所用方法简便快速、准确度高、灵敏度好,适用于测定人血浆中阿哌沙班的浓度,可用于其在健康人体中的药动学及生物等效性研究。

关键词：阿哌沙班抗凝血药超高效液相色谱-串联质谱法血浆浓度蛋白沉淀法药动学生物等效性方法验证