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Journal of Forestry Research 2021年第2期32卷 741-753页

作者： Wei tang Anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 101 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Malonyl-CoA synthetases may modulate cell responses to abiotic stress by regulating stress-related signaling transduction pathways or activating expression of transcription ***,the molecular mechanism of cold stress tolerance enhanced by malonyl-CoA synthetase is not fully ***,we report that overexpression of the Arabidopsis thaliana malonyl-CoA synthetase gene AAE13.1 resulted in increased cell viability and growth rate and decreased thiobarbituric acid reactive substances under cold stress in rice(Oryza sativa L.),tobacco(Nicotiana tabacum),and slash pine(Pinus elliottii Engelm.).AAE13.1 was associated with cold stress tolerance by increasing the activity of ascorbate peroxidase,catalase,polyphenol oxidase,and peroxidase and the accumulation of acid phosphatase and alkaline *** six rice mitogenactivated protein kinase(MAPK)genes examined,AAE13.1 overexpression increased the expression of OsMAPK genes during cold ***13.1 activated expression of stressresponse genes OsMAPK1,OsMAPK2,and OsMAPK3,indicating that AAE13.1 enhances cold stress tolerance by regulating expression of MAPK genes in plant *** results increase our understanding of cold stress tolerance in species of monocotyledons,dicotyledons,and gymnosperms.

关键词： Cold stress Malonyl-CoA synthetase gene MAPK transcription factor Phosphatase Pinus

Overexpression of Arabidopsis thaliana cysteine2/histidine2-type transcription factor 6 gene enhances plant resistance to a bacterial pathogen

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Journal of Forestry Research 2021年第1期32卷 249-262页

作者： Wei tang Anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 2 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Transcription factors can be used to engineer plants for enhanced ***,the mechanism(s)by which the C2H2-type zinc fi nger transcription factor enhances pathogen resistance in cells is not fully ***,Agrobacterium tumefaciens carrying the gene for Arabidopsis thaliana cysteine2/histidine2-type transcription factor 6(ZAT6)was used to engineer rice(Oryza sativa L.),cotton(Gossypium hirsutum L.),and slash pine(Pinus elliottii Engelm.)to generate transgenic cell *** cells were then inoculated with the pathogenic bacterium Pseudomonas *** to the control,cell viability of transgenic cells increased 39–47%and growth rate increased 9–15%by 7 days after inoculation in rice,cotton and slash *** phosphatase activity and alkaline phosphatase activity and transcript levels of Ca 2+-dependent protein kinase genes OsCPK1,OsCPK2,OsCPK6,and OsCPK8 and mitogen-activated protein kinase genes OsMAPK1,OsMAPK2,OsMAPK3,and OsMAPK8 increased signifi cantly in transgenic rice cells by 3 day after inoculation,and extracellular pH had decreased by 10–14%by 96 min after inoculation in transgenic rice,cotton and slash pine *** results suggest that ZAT6 enhances *** resistance in plant cells by modulating transcription of CPK and MAPK and oxidase activity.

关键词： Ca^2+-dependent protein kinase Mitogenactivated protein kinase Oxidase Pathogen Pinus

Genome engineering technologies for targeted genetic modification in plants

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Journal of Forestry Research 2018年第4期29卷 875-887页

作者： Wei tang Anna y. tang College of Arts and Science East Carolina University Greenville NC 27858 USA University of North Carolina at Chapel Hill 101 Stadium Drive Chapel Hill NC 27514 USA

Well-established targeted technologies to engi- neer genomes such as zinc-finger nuclease-based editing （ZFN）, transcription activator-like effector nuclease-based editing （TALEN）, and clustered regularly interspaced short palindromic repeats and associated protein system-based editing （CRISPR/Cas） are proving to advance basic and applied research in numerous plant species. Compared with systems using ZFNs and TALENs, the most recently developed CRISPR/Cas system is more efficient due to its use of an RNA-guided nuclease to generate double-strand DNA breaks. To accelerate the applications of these technologies, we provide here a detailed overview of these systems, highlight the strengths and weaknesses of each, summarize research advances made with these technologies in model and crop plants, and discuss their applications in plant functional genomics. Such targeted approaches for genetically modifying plants will benefit agricultural production in the future.

关键词： Double-stranded DNA break Genomeediting CRISPR system Transcription activator-likeeffector nucleases Zinc-finger nucleases

MicroRNAs associated with molecular mechanisms for plant root formation and growth

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Journal of Forestry Research 2016年第1期27卷 1-12页

作者： Wei tang Anna y.tang Department of Biology East Carolina University 101 Stadium Drive University of North Carolina at Chapel Hill

Although roots are so important for plant growth and crop productivity, the molecular mecha- nism（s） of root formation and growth is not fully under- stood. To increase our understanding of the underlying molecular mechanisms, here we review microRNA regu- lation of processes related to root formation： the regulation of transcription factors, nutrient uptake, stress signaling, and growth signaling. We have summarized the interaction, expression, transport, and signaling events that involve microRNAs in ideal and stressed conditions in a number of model plants, highlighting the involvement of microRNAs in root formation. MicroRNAs are now known to be important players in root initiation, development, and growth; understanding the precise mechanisms involved will be valuable for plant molecular breeding to develop high-yielding crops with high stress resistance and low nutrient requirements.

关键词： MicroRNAs, Nutrient. Root formationStress response Transcription factor

Transcriptional mechanisms regulating gene expression and determining cell fates in plant development

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Journal of Forestry Research 2017年第5期28卷 863-880页

作者： Wei tang Anna y.tang College of Arts and Science East Carolina University University of North Carolina at Chapel Hill

Transcriptional regulatory mechanisms that control transcriptional regulators, target genes, and their interactions provide new insights into general development processes throughout the life cycle of the plant. Although different molecular mechanisms that regulate plant growth and development have been identified, detailed transcriptional mechanisms that control gene expression, modulate developmental programmes, and determine cell fates in plant development are not fully understood. To increase our understanding on transcriptional mechanisms regulating diverse processes in plant development, we have reviewed the regulation of transcription during the process of development including transcriptional mechanisms regulating root, stem, leaf, flower, seed, embryo, endosperm, ovule, fruit, and chloroplast development. We have summarized the interaction, expression, transport, signaling events of transcriptional regulators and their targets in a number of model plants and highlighted the involvement of hormones and microRNAs in plant development. Understanding the precise transcriptional mechanisms regulating gene expression in plant development will be valuable for plant molecular breeding.

关键词： MicroRNAs Molecular breeding Plant development Root formation Transcriptional regulators

Biological significance of RNA-seq and single-cell genomic research in woody plants

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Journal of Forestry Research 2019年第5期30卷 1555-1568页

作者： Wei tang Anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA University of North Carolina at Chapel Hill 101 Stadium DriveChapel HillNC 27514USA

RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living *** knowledge gained from RNA-seq and single-cell genomic research will have a great impact in many aspects of plant *** this review,we summary and discuss the biological significance of RNA-seq and single-cell genomic research in plants including the single-cell DNA-sequencing,RNA-seq and single-cell RNA sequencing in woody plants,methods of RNA-seq and single-cell RNA-sequencing,single-cell RNA-sequencing for studying plant development,and single-cell RNA-sequencing for elucidating cell type *** will focus on RNA-seq and single-cell RNA sequencing in woody plants,understanding of plant development through single-cell RNAsequencing,and elucidation of cell type composition via single-cell *** presented in this review will be helpful to increase our understanding of plant genomic research in a way with the power of plant single-cell RNA-sequencing analysis.

关键词： Cell type composition Genomics Plant development Single-cell RNA-sequencing methods

Transgenic woody plants for biofuel

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Journal of Forestry Research 2014年第2期25卷 225-236页

作者： Wei tang Anna y.tang Department of Biology East Carolina University 101 Stadium Drive University of North Carolina at Chapel Hill

Transgenic trees as a new source for biofuel have brought a great interest in tree biotechnology. Genetically modifying forest trees for ethanol production have advantages in technical challenges, costs, environmental concerns, and financial problems over some of crops. Genetic engineering of forest trees can be used to reduce the level of lignin, to produce the fast-growing trees, to develop trees with higher cellulose, and to allow the trees to be grown more widely. Trees can establish themselves in the field with less care of farmers, compared to most of crops. Transgenic crops as a new source for biofuel have been recently reviewed in several reviews. Here, we overview transgenic woody plants as a new source for biofuel including genetically modified woody plants and environment; main focus of woody plants genetic modifications; solar to chemical energy transfer; cellulose biosynthesis; lignin biosynthesis; and cellulosic ethanol as biofuel.

关键词： biofuel cell wall cellulose lignin transgenic tree

Applications and roles of the CRISPR system in genome editing of plants

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Journal of Forestry Research 2017年第1期28卷 15-28页

作者： Wei tang Anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA University of North Carolina at Chapel Hill 101 Stadium DriveChapel HillNC 27514USA

Genome editing is a valuable tool to target specific DNA sequences for mutagenesis in the genomes of microbes, plants, and animals. Although different genome editing technologies are available, the clustered regularly interspaced short palindromic repeats/Cas9 （CRISPR/ Cas9） system, which utilizes engineered endonucleases to generate a double-stranded DNA break （DSB） in the target DNA region and subsequently stimulates site-specific mutagenesis through DNA repair machineries, is emerging as a powerful genome editing tool for elucidating mecha- nisms of protection from plant viruses, plant disease resistance, and gene functions in basic and applied research. In this review, we provide an overview of recent advances in the CRISPR system associated genome editing in plants by focusing on application of this technology in model plants, crop plants, fruit plants, woody plants and grasses and discuss how genome editing associated with the CRISPR system can provide insights into genome modifications and functional genomics in plants.

关键词： CRISPR system break Functional genomics modifications Double-stranded DNA Genome editing Genome

Novel polyethyleneimine/TMC-based nanofiltration membrane prepared on a polydopamine coated substrate

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Frontiers of Chemical Science and Engineering 2018年第2期12卷 273-282页

作者： Zhe yang Xiaoyu Huang Jianqiang Wang Chuyang y. tang Department of Civil Engineering the University of Hong Kong Hong Kong China

Most commercial NF membranes are negatively charged at the pH range of a typical feed solution. In order to enhance the removal of cations （such as Mg2＋ or Ca2＋）, we utilized polyethyleneimine （PEI） and trimesoyl chloride （TMC） to perform interfacial polymerization reaction on a polydopamine coated hydrolyzed polyacry-lonitrile substrate to obtain a positively charged nanofiltration membrane. Effects ofpolydopamine coating time, PEI concentration, TMC reaction time and concentration on the membrane physicochemical properties and separation performance were systematically investigated using scanning electron microscopy, streaming potential and water contact angle measurements. The optimal NF membrane showed high rejection for divalent ions （93.6±2.6% for MgSO4, 92.4±1.3% for MgCl2, and 90.4±2.1% for Na2SO4）, accompanied with NaCl rejection of 27.8±2.5% with a permeation flux of 17.2±2.8 L.m-2.h-1 at an applied pressure of 8 bar （salt concentrations were all 1000 mg.L-1）. The synthesized membranes showed promising potentials for the applications of water soft-ening.

关键词： nanofiltration polyethyleneimine trimesoyl chloride polydopamine positively charged rejection layer

Nonlinear excitations and“peakons”of a(2+1)-dimensional generalized Broer-Kaup system

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Acta Mechanica Sinica 2007年第2期23卷 209-214页

作者： X. y. tang K. W. Chow S. y. Lou Department of Physics Shanghai Jiao Tong UniversityShanghai 200030 China Department of Mechanical Engineering University of Hong KongHong Kong China Department of Physics Ningbo UniversityNingbo 315211 China

Shallow water waves and a host of long wave phenomena are commonly investigated by various models of nonlinear evolution equations. Examples include the Korteweg-de Vries, the Camassa-Holm, and the Whitham-Broer-Kaup （WBK） equations. Here a generalized WBK system is studied via the multi-linear variable separation approach. A special class of wave profiles with discontinuous derivatives （＂peakons＂） is developed. Peakons of various features, e.g. periodic, pulsating or fractal, are investigated and interactions of such entities are studied.

关键词： Shallow water wave .Excitation Broer-Kaup system .Peakon