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RLINl,encoding a putative coproporphyrinogen Ⅲ oxidase,is involved in lesion initiation in rice

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Journal of Genetics and Genomics 2011年第1期38卷 29-37页

作者： Changhui Sun Linchuan Liu jiuyou tang Aihong Lin Fantao Zhang Jun Fang Genfa Zhang Chengcai Chu Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875 China The State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Datun Road Chaoyang District Beijing 100101 China Graduate School of the Chinese Academy of Sciences Yuquan Road Beijing 100039 China Rice Research Institute Sichuan Agricultural University Chengdu 611130 China

Lesion mimic is necrotic lesions on plant leaf or stem in the absence of pathogenic infection, and its exact biological mechanism is varied. By a large-scale screening of our T-DNA mutant population, we identified a mutant rice lesion initiation 1 （rlin1）, which was controlled by a single nuclear recessive gene. Map-based cloning revealed that RLIN1 encoded a putative coproporphyrinogen Ⅲ oxidase in tetrapyrrole biosynthesis pathway. Sequencing results showed that a G to T substitution occurred in the second exon of RLIN1 and led to a missense mutation from Asp to Tyr. Ectopic expression of RLIN1 could rescue rlin1 lesion mimic phenotype. Histochemical analysis demonstrated that lesion formation in rlin1 was light-dependent accompanied by reactive oxygen species accumulated. These results suggest that tetrapyrrole participates in lesion formation in rice.

关键词： Rice Map-based cloning Lesion mimic Coproporphyrinogen Ⅲ oxidase Tetrapyrrole

An activated form of NB-ARC protein RLS1 functions with cysteine-rich receptor-like protein RMC to trigger cell death in rice

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Plant Communications 2023年第2期4卷 151-166页

作者： Yiqin Wang Zhenfeng Teng Hua Li Wei Wang Fan Xu Kai Sun Jinfang Chu Yangwen Qian Gary JLoake Chengcai Chu jiuyou tang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Genetics and Developmental Biology and National Center for Plant Gene Research(Beijing) Chinese Academy of SciencesBeijing 100101China Biogle Genome Editing Center Changzhou 213125China Institute of Molecular Plant Sciences School of Biological SciencesUniversity of EdinburghEdinburgh EH93BFUK Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China

A key event that follows pathogen recognition by a resistance(R)protein containing an NB-ARC(nucleotide-binding adaptor shared by Apaf-1,R proteins,and Ced-4)domain is hypersensitive response(HR)-type cell death accompanied by accumulation of reactive oxygen species and nitric ***,the integral mechanisms that underlie this process remain relatively ***,we show that a gain-offunction mutation in the NB-ARC protein RLS1(Rapid Leaf Senescence 1)triggers high-light-dependent HR-like cell death in *** RLS1-mediated defense response is largely independent of salicylic acid accumulation,NPR1(Nonexpressor of Pathogenesis-Related Gene 1)activity,and RAR1(Required for Mla12 Resistance 1)function.A screen for suppressors of RLS1 activation identified RMC(Root Meander Curling)as essential for the RLS1-activated defense *** encodes a cysteine-rich receptor-like secreted protein(CRRSP)and functions as an RLS1-binding ***,their co-expression resulted in a change in the pattern of subcellular localization and was sufficient to trigger cell death accompanied by a decrease in the activity of the antioxidant enzyme ***,our findings reveal an NBARC-CRRSP signaling module that modulates oxidative state,the cell death process,and associated immunity responses in rice.

关键词： hypersensitive response NB-ARC protein cysteine-rich receptor-like secreted protein disease resistance antioxidant enzymes

Significant Improvement of Cotton Verticillium Wilt Resistance by Manipulating the Expression of Gastrodia Anlffungal Proteins

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Molecular Plant 2016年第10期9卷 1436-1439页

作者： Yiqin Wang Chengzhen Liang Shenjie Wu Xueyan Zhang jiuyou tang Guijiang Jian Gaili Jiao Fuguang Li Chengcai Chu State Key Laboratory of Plant Genomics and National Center for Plant GeneResearch (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China Institute of Economic Crops Shanxi Academy of Agricultural Sciences Yuncheng Shanxi Province 044000 China Institute of Cotton Research Chinese Academy of Agricultural Sciences Anyang Henan Province 455000 China Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China

Dear Editor Verticillium wilt caused by Verticillium dahliae Kleb. is the most destructive disease in cotton-growing areas around the world. The infection significantly reduces cotton yield and fiber quality due to leaf chlorosis, necrosis or wilting, leaf or boll abscission, and even plant death. The losses may reach up to 80% of lint cotton yield （Wei et al., 2015）.

关键词： GASTRODIA elata VERTICILLIUM wilt of cotton DISEASE resistance of plants

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

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Molecular Plant 2017年第9期10卷 1242-1245页

作者： Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei tang, jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai Qian, Yangwen Biogle Genome Editing Ctr Changzhou 213125 Jiangsu Peoples R China Chinese Acad Sci Natl Ctr Plant Gene Res Beijing Inst Genet & Dev Biol State Key Lab Plant Genom Beijing 100101 Peoples R China Chinese Acad Sci Shanghai Ctr Plant Stress Biol Shanghai 201602 Peoples R China Chinese Acad Sci Ctr Excellence Mol Plant Sci Shanghai 201602 Peoples R China Chinese Acad Sci Inst Subtrop Agr Key Lab Agroecol Proc Subtrop Reg Changsha 410125 Hunan Peoples R China

Dear Editor Since the completion of the rice （Oryza sativa） genome- sequencing project, a major goal of rice research has been the functional characterization of all annotated genetic loci in various biological processes. One of the most efficient and widely-used strategies for studying gene function is genetic mutagenesis. Several rice mutant libraries have been generated in the past decade, providing a wealth of resources for plant research （Chang et al., 2012）. CRISPR/Cas9 （clustered regularly interspaced short palindromic repeats-associated nuclease 9） has recently emerged as a powerful tool for rice research and breeding （Cong et al., 2013; Feng et al., 2013; Miao et al., 2013;

关键词： Genome-wide Targeted Mutagenesis Rice Using the CRISPR Cas9 System

Genomic decoding of breeding history to guide breeding-by-design in rice

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National Science Review 2023年第5期10卷 124-137页

作者： Zhuo Chen Qingyun Bu Guifu Liu Maoqing Wang Hongru Wang Huazhao Liu Xiufeng Li Hong Li Jun Fang Yan Liang Zhenfeng Teng Sai Kang Hong Yu Zhukuan Cheng Yongbiao Xue Chengzhi Liang jiuyou tang Jiayang Li Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of Sciences Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and AgroecologyChinese Academy of Sciences Key Laboratory of Crop Biotechnology Breeding of the Ministry of Agriculture and Rural Affairs Beidahuang Kenfeng Seed Co.Ltd Institute of Botany Chinese Academy of Sciences Guangdong Laboratory for Lingnan Modern Agriculture and State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of AgricultureSouth China Agricultural University University of Chinese Academy of Sciences State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of Sciences Hainan Yazhou Bay Seed Laboratory

Deciphering the intrinsic molecular logic of empirical crop breeding from a genomic perspective is a decisive prerequisite for breeding-by-design(BbD),but remains not well ***,we decoded the historical features of past rice breeding by phenotyping and haplotyping 546 accessions covering the majority of cultivars bred in the history of Northeast China(NEC).We revealed that three groups founded the genetic diversities in NEC rice with distinct evolution patterns and traced and verified the breeding footprints to known or genome-wide association study(GWAS)-detected quantitative trait loci(QTLs),or introgressions from indica sub-species with chronological changes in allele *** we summarized a rice breeding trend/principle in NEC,and combined with the successful example in breeding and application of Zhongkefa5 to demonstrate the guiding value of our conclusion for BbD in *** study provides a paradigm for decoding the breeding history of a specific crop to guide BbD,which may have implications in different crop breeding.

关键词： rice breeding-by-design breeding history genome-wide association study genomic decoding

Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system

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Journal of Genetics and Genomics 2009年第5期36卷 267-276页

作者： Yimian Ma Luo Liu Chengguang Zhu Changhui Sun Bo Xu Jun Fang jiuyou tang Anding Luo Shouyun Cao Gupo Li Qian Qian Yongbiao Xue Chengcai Chu State Key Laboratory of Plant Genomics and National Plant Gene Research Center (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate University of the Chinese Academy of Sciences Beijing 100039 China State Key Laboratory of Rice Biology China Institute of Rice Research Chinese Academy of Agricultural Sciences Hangzhou 310006 China Laboratory of Molecular and Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.

关键词： rice mutant population promoter trap activation tag T-DNA integration

SDG724 mediates H3K36me2/3 deposition at MADS50 and RFT1,and promotes flowering in rice

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SDG724 mediates H3K36me2/3 deposition at MADS50 and RFT1,and...

The Inheritance of Early Heading in the Rice Variety USSR5

从植物科学到农业发展——2012全国植物生物学大会

作者： Changhui Sun,Jun Fang,Taolan Zhao,Bo Xu,Fantao Zhang,Linchuan Liu,jiuyou tang, Xiaojian Deng,Fan Chen,Qian Qian,Xiaofeng Cao,Chengcai Chu Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China.

Chromatin modifications are known to affect flowering time in the long-day(LD) plant ***,the role of histone methylation in flowering time regulation of rice, a short-day(SD) plant,remains to be *** identified a late-flowering long vegetative phase 1(lvp1) mutant in rice,and map-based cloning revealed that LVP1 encodes the SET domain group protein 724(SDG724).Biochemical characterization showed that SDG724 is an active histone methyltransferase in vitro and contributes to a major fraction of global H3K36 methylation in *** analyses of flowering time genes in wild-type and lvp1 mutants revealed that Ehd1,but not Hd1,are misregulated in SDG724 loss of function mutants to promote flowering under both SD and LD conditions. In addition,the lvp1 se5 double mutant flowered later than the se5 single mutant,indicating that lesions in SDG724 delay flowering time irrespective of *** assays showed that Ivp1 had reduced levels of H3K36me2/3 within certain chromatin regions of Os MADS50 and *** suggests that the divergent functions of paralogs RFT1 and Hd3a,and of Os MADS50 and MADS51 are in part due to differential H3K36me2/3 deposition,which also correlates with higher expression levels of Os MADS50 and RFT1 in flowering promotion of rice.

关键词： rice flowering transition histone methyltransferase H3K36 methylation florigen

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Journal of Genetics and Genomics 2007年第1期34卷 46-55页

作者：江玲徐俊锋魏祥进王松凤唐九友翟虎渠万建民南京农业大学作物遗传与种质创新国家重点实验室江苏省植物基因工程技术研究中心中国农业科学院作物科学研究所北京100081

USSR5, a japonica rice variety from the former Soviet Union, is an extremely early maturing rice variety. To elucidate the genetic basis for its early heading, genetic analysis was carried out by crossing it with a set of major gene nearly isogenic lines （NIL） and QTL-isogenic lines. The early heading of USSR5 was attributed to the presence of photoperiod-insensitive alleles at E1 and Se-1 gene, the photoperiod-sensitive inhibitor gene i-Se-1, and the dominant earliness gene Ef-1. Analysis of a backcrossed population （BCIF1） derived from the cross USSR5 x N22 indicated that two quantitative trait loci （QTL） for early heading were located on chromosomes 7 and 8, accounting for 27.4% and 11.2% of the phenotypic variance, respectively, with both early alleles originating from USSRS. From an F2 population of the same cross, early heading QTLs were detected on chromosomes 1, 2, 7, 9, and 10, with individual QTL accounting for between 4.1% and 15.4% of the phenotypic variance. Early heading alleles at four of these five QTLs originated from USSRS. A comparison of chromosomal locations suggests that one of these QTLs may be identical with the known gene Hd4 （E1）. The relationship between the other QTLs and known genes for heading date are not clear. USSR5 is a promising source for propagating earliness for the development of improved early heading rice varieties.

关键词： rice (Oryza sativa L.) heading date nearly isogenic line genotype analysis QTL mapping

Impact of ocean data assimilation on the seasonal forecast of the 2014/15 marine heatwave in the Northeast Pacific Ocean

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 24-31页

作者： Tiantian tang Jiaying He Huihang Sun Jingjia Luo Institute for Climate and Application Research(ICAR)/Key Laboratory of Meteorological Disaster Nanjing University of Information Science and TechnologyNanjingChina

A remarkable marine heatwave,known as the“Blob”,occurred in the Northeast Pacific Ocean from late 2013 to early 2016,which displayed strong warm anomalies extending from the surface to a depth of 300 *** study employed two assimilation schemes based on the global Climate Forecast System of Nanjing University of Information Science(NUIST-CFS 1.0)to investigate the impact of ocean data assimilation on the seasonal prediction of this extreme marine *** sea surface temperature(SST)nudging scheme assimilates SST only,while the deterministic ensemble Kalman filter(EnKF)scheme assimilates observations from the surface to the deep *** latter notably improves the forecasting skill for subsurface temperature anomalies,especially at the depth of 100-300 m(the lower layer),outperforming the SST nudging *** excels in predicting both horizontal and vertical heat transport in the lower layer,contributing to improved forecasts of the lower-layer warming during the *** improvements stem from the assimilation of subsurface observational data,which are important in predicting the upper-ocean *** results suggest that assimilating ocean data with the EnKF scheme significantly enhances the accuracy in predicting subsurface temperature anomalies during the Blob and offers better understanding of its underlying mechanisms.

关键词： Seasonal forecast Ocean data assimilation Marine heatwave Subsurface temperature