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Chinese Science Bulletin 2001年第1期46卷 53-57页

作者： REN Hongwei Muto Norio Itoh Norio YU Meimin RU Binggeng tanaka keiichi National Laboratory of Protein Engineering College of Life SciencesPeking UniversityBeijing 100871China Department of Environmental Toxicology Faculty of Pharmaceutical SciencesOsaka University1-6 YamadaokaSuitaOsaka 565-0871Japan

Generally, there are two major isoforms of me-tallothionein (MT) in mammals. In this study two cDNAs of metallothionein, MT-A and MT-B, in a fresh-water fish, crucian carp (Carassius cuvieri), were cloned by the rapid amplification of cDNA ends (RACE). The homology of their reading frame is about 92.3%. The sequence analysis of both cDNAs gave the structures of coding regions corresponding to 60 amino acid residues, and the structures of complete 3’ -untranslated regions in which a significant difference in the size of their 3’-untranslated regions (130 bp for MT-A and 280 bp for MT-B) exists. The results of amino acid sequencing of both MT-1 and MT-2 purified by HPLC are identical to those deduced from MT cDNA genes, indicating that MT-1 is from MT-A gene and MT-2 is from MT-B gene respectively. No blocking in the N-terminal of MT-2 isoform was the first case found in vertebrates, most of which were blocked by acetylation. These results suggest that there were differential controls at the

关键词： metallothionein isoform carassius-cuvieri suppression RACE.

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Gastrointestinal stromal tumor presenting with prominent calcification

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World Journal of Gastroenterology 2012年第39期18卷 5645-5648页

作者： Naoki Izawa Takeshi Sawada Ryuichi Abiko Daisuke Kumon Mami Hirakawa Mika Kobayashi Nobuyuki Obinata Masahito Nomoto Tadateru Maehata Shun-ichi Yamauchi Takefumi Kouro Takashi Tsuda Satoshi Kitajima Hiroshi Yasuda keiichi tanaka Ichiro tanaka Masahiro Hoshikawa Masayuki Takagi Fumio Itoh Division of Gastroenterology and Hepatology St.Marianna University School of MedicineKawasaki 216-8511Japan Division of Gastroenterological and General Surgery St.Marianna University School of MedicineKawasaki 216-8511Japan Department of Pathology St.Marianna University School of MedicineKawasak 216-8511Japan

We present a rare case of a gastrointestinal stromal tumor (GIST) in the stomach with prominent calcifica-tion at presentation. A 61-year-old woman visited our hospital because of epigastric discomfort. A spherical calcified lesion with a diameter of about 30 mm was incidentally shown in the left upper quadrant on an abdominal X-ray. Computed tomography demonstrated that the tumor was growing from the upper gastric body, with calcification in the peripheral ring area. A laparoscopic partial gastrectomy was performed, and the resected specimen revealed a well-circumscribed tumor with exophytic growth from the gastric muscularis propria. Microscopic examination revealed spindle- shaped tumor cells with calcification and hemorrhage. Additionally, positive immunoreactivity of the tumor to KIT and CD34 and a low mitotic index resulted in the diagnosis of very low risk GIST. There are a few case reports of heavily calcified GIST, although solitary or punctate calcification of primary GIST has been reported in several case series. Dystrophic calcification of necrotic or degenerative tissue is the supposed cause of primary calcified GISTs. In contrast, appearance of calcification after administration of imatinib mesylate, which may be one indicator of disease response, is possibly caused by a different mechanism.

关键词： Gastrointestinal stromal tumor Calcifica-tion Stomach Computed tomography Imatinib me-sylate

Iguratimod, a Disease-Modifying Anti-Rheumatic Drug, Inhibits Osteoclastogenesis and Bone Resorption through Suppression of the Nuclear Factor of Activated T Cells Signaling Pathway

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Open Journal of Rheumatology and Autoimmune Diseases 2016年第4期6卷 106-119页

作者： Jun Shiota Hidetoshi Murao Akihiko Miura Masaaki Mikami keiichi tanaka Research Laboratories Toyama Chemical Co. Ltd. Toyama Japan

Introduction: The aim of this study was to observe an inhibition of bone resorption and osteoclastogenesis by iguratimod (IGU, T-614), a disease-modifying anti-rheumatic drug, using adjuvant-induced arthritis (AIA) rats and receptor activator of nuclear factor kappa-B ligand (RANKL)-stimulated RAW264.7 cells. Methods: The bone mineral density and 3D morphometric parameters of hind paws in AIA rats were measured using micro computed tomography (μCT) imaging. The activity of osteoclast cells was estimated based on tartrate-resistant acid phosphatase (TRAP) staining in specimens from the rats. In vitro TRAP activity was investigated using RANKL-stimulated RAW264.7 cells. The amount of nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) protein was measured by western blot analysis. The expression of Nfatc1, its regulator genes, its upstream factors, and osteoclast-functional genes were investigated. Results: In addition to the suppression of bone resorption and lesions of bone trabeculae of AIA rats, IGU significantly decreased the number of TRAP-positive cells in the calcaneal bones. Moreover, this drug inhibited the differentiation of RANKL-stimulated RAW264.7 cells into osteoclasts, which were identified morphologically and functionally. IGU decreased the amount of NFATc1 protein and improved the altered expression of NFATc1-associated genes and osteoclast-functional genes. Conclusions: IGU suppressed osteoclastogenesis and bone resorption via the RANKL-NFATc1 pathway, suggesting such effect would be expected in clinical use.

关键词： Bone Resorption Iguratimod NFATc1 Osteoclast Rheumatoid Arthritis

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乐陶

现代装饰（家居） 2013年第11期 52-55页

作者： keiichi tanaka 伏平

一件完美的陶瓷工艺品的制作加工过程严密而细致。首先是将瓷土淘成可用的瓷泥,并将其分割开来摞成柱状。然后,将摞好的瓷泥放入旋转转盘,用手和拉坯工具将瓷泥拉成瓷坯。拉好的瓷坯只是一个雏形,还需要根据所做的形状选取不同的印模,... 详细信息

一件完美的陶瓷工艺品的制作加工过程严密而细致。首先是将瓷土淘成可用的瓷泥,并将其分割开来摞成柱状。然后,将摞好的瓷泥放入旋转转盘,用手和拉坯工具将瓷泥拉成瓷坯。拉好的瓷坯只是一个雏形,还需要根据所做的形状选取不同的印模,将瓷坯印成各种不同的形状,再将印好的瓷坯修刮整齐与匀称,用清水洗去尘土。然后,画坯、上釉与烧窑。瓷坯在窑内经受千度高温的烧炼后,最终成为精美的瓷器。来自日本的陶瓷艺术家keiichi tanaka,1979年出生于日本千叶,1999年就读于日本武藏野美术大学工业系室内与工艺美术设计专业,2003年毕业后留校,担任助教、助理研究员。

关键词：陶瓷艺术家画坯烧窑武藏野拉坯上釉制作加工工艺美术设计日本千叶 tanaka

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毫微米级机械振幅的激光干涉测量法

中国检验检测 1980年第3期 43-46+56页

作者： keiichi tanaka 曾稳盛

一、前言早已报导过用干涉法测量微小振幅。Osterberg 提出了应用单色干涉条纹并以一个反射镜产生正弦运动方法的数学原理。用这种方法,按照光波波长可测量绝对振幅。然而,根据理论,通过直接观测条纹能测出的最小振幅限制到0.191λ(λ... 详细信息

一、前言早已报导过用干涉法测量微小振幅。Osterberg 提出了应用单色干涉条纹并以一个反射镜产生正弦运动方法的数学原理。用这种方法,按照光波波长可测量绝对振幅。然而,根据理论,通过直接观测条纹能测出的最小振幅限制到0.191λ(λ为光波波长)。可用干涉仪由一对平镜组成,由准直的光束照射。为了

关键词：反射镜正弦输入信号单次平镜干涉测量法反光镜超声波传感器

Preliminary Result of Hyperfractionated High-Dose Proton Beam Radiotherapy for Pediatric Skull Base Chordomas

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Journal of Cancer Therapy 2017年第4期8卷 327-332页

作者： Masashi Mizumoto Hiroyoshi Akutsu Tetsuya Yamamoto Takashi Fukushima Yoshiko Oshiro Daichi Takizawa keiichi tanaka Masaaki Goto Toshiyuki Okumura Akira Matsumura Koji Tsuboi Hideyuki Sakurai Departments of Radiation Oncology University of Tsukuba Tsukuba Japan Departments of Neurosurgery University of Tsukuba Tsukuba Japan Departments of Child Health University of Tsukuba Tsukuba Japan

Objective: Proton beam therapy (PBT) may provide good local control for skull base chordoma and reduced toxicities, especially for pediatric patients. Methods: We evaluated the efficacy and safety of hyperfractionated high-dose PBT in6 pediatric patients with newly-diagnosed skull basechordoma who were treated with PBT at our institute from 2011 to 2015. The patients were 5 males and one female, and the median age was 9 years old (range: 5 - 13). All patients received surgery before PBT. The median period between surgery and PBT was 57 days (range: 34 - 129 days). The treatment dose was 78.4 GyE in 56 fractions (twice per day). Results: All patients received PBT without severe acute toxicity. The median follow-up period was 27 months (range: 21 - 71 months). At the last follow-up, all patients were alive and all tumors were well controlled. Acute and late toxicities were generally acceptable, with only grade 1 and 2 events. Late toxicities included growth hormone abnormality and cortical hormone abnormality. One patient needed growth hormone and cortical hormone replacement therapy. Conclusion: Although the number of pediatric patients was small, our overall findings in the 6 cases indicate that hyperfractionated high-dose PBT is safe and effective for pediatric patients with skull base chordoma.

关键词： Chordoma Radiotherapy Proton Beam Therapy Proton Radiotherapy Pediatrics

肉鸭的体脂肪含量与血清脂类成分变化规律的研究

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动物营养学报 2005年第1期17卷 19-19页

作者：周长海田中桂一吉林大学生物与农业工程学院吉林长春130022 日本北海道大学农学部

试验测定了肉鸭不同生长阶段体脂肪含量及血清中各种脂类成分含量。结果表明,伴随肉鸭日龄的增加,肉鸭腹腔脂肪含量及体脂肪含量逐渐增加,血清中酯型胆固醇和游离型胆固醇含量递减,三磷酸甘油酯递增,磷脂无规律性变化。说明腹腔脂肪含... 详细信息

试验测定了肉鸭不同生长阶段体脂肪含量及血清中各种脂类成分含量。结果表明,伴随肉鸭日龄的增加,肉鸭腹腔脂肪含量及体脂肪含量逐渐增加,血清中酯型胆固醇和游离型胆固醇含量递减,三磷酸甘油酯递增,磷脂无规律性变化。说明腹腔脂肪含量是判断体脂肪积累状况的重要指标。血清中三磷酸甘油酯浓度不仅与体脂肪含量成比较强的正相关,而且可以作为肉鸭体脂肪合成与积累的判断指标。

关键词：脂肪含量肉鸭血清变化规律脂类胆固醇含量成分含量生长阶段试验测定脂肪积累判断指标脂肪合成甘油酯三磷酸游离型规律性正相关腹腔日龄

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Isolated splenic vein thrombosis secondary to splenic metastasis: A case report

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World Journal of Gastroenterology 2006年第40期12卷 6561-6563页

作者： Kunihiko Hiraiwa Kyoei Morozumi Hiroshi Miyazaki keiichi Sotome Akio Fu-rukawa Makoto Nakamaru Yoichi tanaka Hisami Iri Department of Surgery Hamamatsu Red Cross Hospital Hamamatsu Shizuoka Japan Department of Surgery Fussa Hos-pital Fussa Tokyo Japan Division of Surgical Pathology Clinical Labora-tory Tokyo Dental College Ichikawa General Hospital Ichikawa Chiba Japan Department of Pathology Fussa Hospital Fussa To-kyo Japan

A 49-year-old, previously healthy woman sought treatment for abdominal pain. Colonoscopy revealed ascending colon cancer. Computed tomography and angiography showed splenic metastasis and thrombosis extending from the splenic vein to the portal vein. She underwent right hemicolectomy, splenectomy, and distal pancreatomy. Histological findings showed no malignant cell in the splenic vein which was filled with organizing thrombus. We postulate the mechanism of splenic vein thrombosis in our case to be secondary to the extrinsic compression of the splenic vein by the splenic metastasis or by the inflammatory process produced by the splenic metastasis. In conclusion, we suggest that splenic metastasis should be added to the list of differential diagnosis which causes splenic vein thrombosis. In the absence of other sites of neoplastic disease, splenectomy seems to be the preferred therapy because it can be performed with low morbidity and harbors the potential for long-term survival.

关键词： Colon cancer Splenic metastasis Splenic vein occlusion Splenic vein thrombosis

Clinical efficacy of a combination of Percoll continuous density gradient and swim-up techniques for semen processing in HIV-1 serodiscordant couples

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Asian Journal of Andrology 2017年第2期19卷 208-213页

作者： Osamu Inoue Naoaki Kuji Hiroe Ito Mitsutoshi Yamada Toshio Hamatani Aimi Oyadomari Shingo Kato Hideji Hanabusa keiichi Isaka Mamoru tanaka Department of Obstetrics and Gynecology Keio University School of Medicine Tokyo 1608582 Japan Department of Obstetrics and Gynecology Tokyo Medical College Tokyo 1600023 Japan Department of Microbiology Keio University School of Medicine Tokyo 1608582 Japan Department of Hematology Ogikubo Hospital Tokyo 1670035 Japan

To evaluate the clinical efficacy of a procedure comprising a combination of Percoll continuous density gradient and modified swim-up techniques for the removal of human immunodeficiency virus type 1 （HIV-1） from the semen of HIV-1 infected males, a total of 129 couples with an HIV-1 positive male partner and an HIV-1 negative female partner （serodiscordant couples） who were treated at Keio University Hospital between January 2002 and April 2012 were examined. A total of 183 ejaculates from 129 HIV-1 infected males were processed. After swim-up, we successfully collected motile sperms at a recovery rate as high as 100.0% in cases of normozoospermia （126/126 ejaculates）, oligozoospermia （6/6）, and asthenozoospermia （36/36）. The recovery rate of oligoasthenozoospermia was 86.7% （13/15）. In processed semen only four ejaculates （4/181：2.2%） showed viral nucleotide sequences consistent with those in the blood of the infected males. After using these sperms, no horizontal infections of the female patients and no vertical infections of the newborns were observed. Furthermore, no obvious adverse effects were observed in the offspring. This protocol allowed us to collect HIV-1 negative motile sperms at a high rate, even in male factor cases. We concluded that our protocol is clinically effective both for decreasing HIV-1 infections and for yielding a healthy child.

关键词： assisted reproductive technology human immunodeficiency virus type 1 serodiscordant couPle viral nucleotide sequence

Veno-Venous Extracorporeal Membrane Oxygenation for Acute Lung Injury after Surgery for Aortic Dissection: A Case Report

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World Journal of Cardiovascular Surgery 2017年第12期7卷 156-163页

作者： Takanori Kono Takeshi Oda keiichi Akaiwa Katsuhiko Nakamura Hiroyuki tanaka Department of Cardiovascular Surgery Omura Municipal Hospital Nagasaki Japan Department of Surgery Kurume University School of Medicine Fukuoka Japan

Acute respiratory failure after surgery for aortic dissection is a serious complication that has been associated with increased mortality and morbidity. Veno-venous (VV) extracorporeal membrane oxygenation (ECMO) is reported as one of the treatments for this life-threatening complication. A 78-year-old male patient was admitted to our hospital for Stanford type A acute aortic dissection. He underwent emergency replacement of the ascending aorta, after which he developed acute lung injury. It was difficult to maintain oxygenation with a respirator mask. Therefore, VV-ECMO was initiated on postoperative day 1. The oxygenation gradually improved, and VV-ECMO was continued until postoperative day 13. On postoperative day 25, mechanical ventilation was withdrawn. The patient was discharged from the hospital on postoperative day 149. We report a case of successful treatment of acute lung injury with VV-ECMO initiated after surgery for aortic dissection. VV-ECMO can be considered as a treatment option for severe acute lung injury after surgery for aortic dissection.

关键词： Acute Aortic Dissection Cardiopulmonary Bypass Cardiothoracic Surgery Acute Lung Injury Extracorporeal Membrane Oxygenation