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Glycogen metabolism-mediated intercellular communication in the tumor microenvironment influences liver cancer prognosis

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Oncology Research 2024年第3期32卷 563-576页

作者： YANG ZHANG NANNAN QIN XIJUN WANG RUI LIANG QUAN LIU RUOYI GENG TIANXIAO JIANG yunfei LIU JINWEI LI Graduate School Kunming Medical UniversityKunming650000China Depatment of Vascular Surgery Fuwai Yunnan Cardiovascular HospitalAfliated Cardiovascular Hospital of Kunming Medical UniversityKunming650000China Department of Neurosurgery West China HospitalSichuan UniversityChengdu610000China Department of Neurosurgery The Fourth Afiliated Hospital of Guangxi Medical UniversityLiuzhou545000China Depatment of Gynecology Oncology The Fourth Afliated Hospital of Guangxi Medical UniversityLiuzhou545000China School of Basic Medical Sciences Xianning Medical CollegeHubei University of Science and TechnologyXianning437100China College of Bioengineering Chongqing UniversityChongqing400030China Department of General Visceraland Transplant SurgeryLudwig Maximilians University MunichMunich81377Germany

Glycogen metabolism plays a key role in the development of hepatoellular carcinoma(HCC),but the function of glycogen metabolism genes in the tumor microenvironment(TME)is still to be *** cell RNA-seq data were obtained from ten HCC tumor samples totaling 64,545 cells and 65 glycogen metabolism genes were analyzed bya nonnegative matrix factorization(NMF).The prognosis and immune response of new glycogen TME cell dusters were predicted by using HCC and immunotherapy cohorts from public *** single cell analysis was divided into fibroblasts,NT T cells,macrophages,endothelial clls,and B cells,which were separately divided into new cell clusters by glycogen metabolism gene *** temporal trajectory analysis demonstrated the temporal differentiation trajectory of different glycogen subtype cell *** communication analysis revealed extensive interactions between endothelial cells with glycogen metabolizing TME *** subtypes and diferent glycogen subtype cell *** analysis of transcription factors upstream of TME cell clusters with different glycogen *** addition,TME cell dusters of glycogen metabolism were found to be enriched in expression in CAF subtypes,CD8 depleted,M1,and M2 *** seq analysis showed the prognostic signifcance of glycogen *** TME cell dusters in HCC,while a significant immune response was found in the immunotherapy cohort in patients treated with immune checkpoint blockade(ICB),especially for CAFs,T cells,and macrophages In summary,our study reveals for the first time that glycogen metabolism mediates intercellular communication in the hepatocellular carcinoma microenvironment while elucidating the anti-tumor mechanisms and immune prognostic responses of different subtypes of cell dusters.

关键词： Glycogen metabolism Metabolic map Single cell Tumor microenvironment Liver cancer Prognosis Immunotherapy

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GRK2-YAP signaling is implicated in pulmonary arterial hypertension development

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Chinese Medical Journal 2024年第7期137卷 846-858页

作者： Peng Ye yunfei Deng Yue Gu Pengfei Liu Jie Luo Jiangqin Pu Jingyu Chen Yu Huang Nanping Wang Yong Ji Shaoliang Chen Division of Cardiovascular Molecular Laboratory Third Clinical CollegeNanjing Medical UniversityNanjingJiangsu 210006China Division of Cardiovascular Laboratory Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China Division of Cardiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200080China Division of Pulmonary Surgery Wuxi People’s HospitalNanjing Medical UniversityWuxiJiangsu 300247China Institute of Vascular Medicine The Chinese University of Hong KongHongkong 999077China Health Science Center East China Normal UniversityShanghai 200241China School of Pharmacy Nanjing Medical UniversityNanjingJiangsu 210004China Division of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China

Background:Pulmonary arterial hypertension(PAH)is characterized by excessive proliferation of small pulmonary arterial vascular smooth muscle cells(PASMCs),endothelial dysfunction,and extracellular matrix remodeling.G protein-coupled receptor kinase 2(GRK2)plays an important role in the maintenance of vascular tone and blood ***,the role of GRK2 in the pathogenesis of PAH is ***:GRK2 levels were detected in lung tissues from healthy people and PAH patients.C57BL/6 mice,vascular smooth muscle cell-specific Grk2-knockout mice(Grk2^(∆SM22)),and littermate controls(Grk2^(flox/flox))were grouped into control and hypoxia mice(n=8).Pulmonary hypertension(PH)was induced by exposure to chronic hypoxia(10%)combined with injection of the SU5416(cHx/SU).The expression levels of GRK2 and Yes-associated protein(YAP)in pulmonary arteries and PASMCs were detected by Western blotting and immunofluorescence *** mRNA expression levels of Grk2 and Yes-associated protein(YAP)in PASMCs were quantified with real-time polymerase chain reaction(RT-PCR).Wound-healing assay,3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)assay,and 5-Ethynyl-2′-deoxyuridine(EdU)staining were performed to evaluate the proliferation and migration of ***,the interaction among proteins was detected by immunoprecipitation ***:The expression levels of GRK2 were upregulated in the pulmonary arteries of patients with PAH and the lungs of PH ***,cHx/SU-induced PH was attenuated in Grk2^(∆SM22) mice compared with littermate *** amelioration of PH in Grk2^(∆SM22) mice was accompanied by reduced pulmonary vascular *** vitro study further confirmed that GRK2 knock-down significantly altered hypoxia-induced PASMCs proliferation and migration,whereas this effect was severely intensified by overexpression of *** also identified that GRK2 promoted YAP expression and nuclear translocation in PASMCs,resulting in excessive PA

关键词： Pulmonary arterial hypertension GRK2 YAP Pulmonary artery smooth muscle cell Hypoxia

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Adaptive Predefined-Time Backstepping Control for Grid Connected Photovoltaic Inverter

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Energy Engineering 2024年第8期121卷 2065-2083页

作者： Jiarui Zhang Dan Liu Kan Cao Ping Xiong Xiaotong Ji Yanze Xu yunfei Mu Key Laboratory of Smart Grid of Ministry of Education Tianjin UniversityTianjin300072China State Grid Hubei Electric Power Research Institute Wuhan430077China State Grid Hubei Electric Power Co. Ltd.Wuhan430077China

The system performance of grid-connected photovoltaic(PV)has a serious impact on the grid *** improve the control performance and shorten the convergence time,a predefined-time controller based on backstepping technology and dynamic surface control is formulated for the inverter in the grid-connected *** time-varying tuning functions are introduced into state-tracking errors to realize the predefined-time control *** address the“computational explosion problem”in the design process of backstepping control,dynamic surface control is adopted to avoid the analytical calculations of virtual *** disturbances of the PV system are estimated and compensated by adaptive *** control parameters are chosen and the global stability of the closed-loop is ensured by Lyapunov *** results confirm the effectiveness of the proposed controller and ensure the predefined time control in the photovoltaic inverter.

关键词： Photovoltaic inverter system backstepping technology predefined-time control adaptive control

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Ciclopirox inhibits SARS-CoV-2 replication by promoting the degradation of the nucleocapsid protein

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2505-2519页

作者： Xiafei Wei Yuzheng Zhou Xiaotong Shen Lujie Fan Donglan Liu Xiang Gao Jian Zhou Yezi Wu yunfei Li Wei Feng Zheng Zhang Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalSouthern University of Science and TechnologyShenzhen 518112China Guangzhou Laboratory Guangzhou Medical UniversityGuangzhou 511495China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory Healththe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120China

The nucleocapsid protein(NP)plays a crucial role in SARS-CoV-2 replication and is the most abundant structural protein with a long *** its vital role in severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)assembly and host inflammatory response,it remains an unexplored target for drug *** this study,we identified a small-molecule compound(ciclopirox)that promotes NP degradation using an FDA-approved library and a drug-screening cell *** significantly inhibited SARS-CoV-2 replication both in vitro and in vivo by inducing NP *** induced abnormal NP aggregation through indirect interaction,leading to the formation of condensates with higher viscosity and lower *** condensates were subsequently degraded via the autophagy-lysosomal pathway,ultimately resulting in a shortened NP half-life and reduced NP *** results suggest that NP is a potential drug target,and that ciclopirox holds substantial promise for further development to combat SARS-CoV-2 replication.

关键词： SARS-CoV-2 Nucleocapsid protein Viral replication Ciclopirox Abnormal aggregation Protein degradation Autophagy-lysosome Drug target

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CLAWS CELEBRE

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The World of Chinese 2019年第4期 72-73页

作者：谭云飞不详

Last July, 100,000 cooked crayfish from Hubei province boarded the Sino-Euro Railway and landed in Russia for the 2018 World Cup--baffling foreign media, but exhilarating Chinese spectators for whom this summer treat ... 详细信息

关键词： CLAWS CELEBRE Last July crayfish

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FRUIT OF THE SOUTH

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The World of Chinese 2021年第1期 72-73页

作者：谭云飞不详

A steed raising red dust won the fair mistress’ smiles/But few knew how many steeds died bringing her lychees from the south," wrote the poet Du Mu(杜牧) in the tang dynasty (618-907),satirizing Emperor Xuanzong’s r... 详细信息

关键词： favorite bringing raising

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COOL BEANS

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The World of Chinese 2020年第4期 74-75页

作者：谭云飞不详

A starchy snack that relieves the heat of summer凉粉:炎炎夏日的解暑佳品"Sour,salty,spicy,aromatic!More refreshing than ice!A bowl of it would bring you to heaven(酸,咸,麻辣香,解暑胜冰凉,若能吃一碗,犹如进天堂)!"This ch... 详细信息

关键词： starch COOL 凉粉

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FRAUDSTERS ARE EXPLOITING BUREAUCRATIC INERTIA OVER ID FRAUD

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The World of Chinese 2019年第3期 6-7页

作者：谭云飞不详

For nearly four years, a man wanted for murdering his mother evaded police with a series of' false identities. The capture of 24-year-old Wu Xieyu at a Chongqing airport in April, with over 30 national ID cards in his possession, has once more drawn attention to the thriving illegal market in fake identities-and the bureaucracy than enables it.

关键词： man police cards

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Ricec Cispy

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The World of Chinese 2018年第6期 84-85页

作者：谭云飞不详

Rural children born in China during the 1970s and 80s can perhaps recall standing at grandma's elbow as she cooked steaming rice over firewood, hoping she'll let them scrape the pot if they behaved--a childhood tradit... 详细信息

关键词： Rural children steaming rice similar

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TECHNO BABBLE

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The World of Chinese 2020年第4期 88-90页

作者：谭云飞不详

The incomprehensible jargon of Chinese internet firms当生活充满互联网行话"If the last 20 years have been about internet technology,the next 30 years could be called the internet era,"Jack Ma,the founder of Chinese int... 详细信息

关键词： internet Transformation fir

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