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槲蕨根茎响应钙胁迫的差异表达代谢物分析

广西植物 2024年第3期44卷 531-540页

作者：吴依琳李慧马洪娜李伟忠檀龙颜贵州中医药大学药学院贵阳550025

槲蕨为喀斯特地区典型的附生蕨类植物,主要通过其根茎附生于岩石的表面或缝隙中。槲蕨以干燥的根茎入药,具有重要的药用价值。为探究钙离子(Ca^(2+))胁迫下槲蕨根茎中代谢物的变化,该研究采用超高效液相色谱联用四极杆飞行时间质谱(UPLC... 详细信息

槲蕨为喀斯特地区典型的附生蕨类植物,主要通过其根茎附生于岩石的表面或缝隙中。槲蕨以干燥的根茎入药,具有重要的药用价值。为探究钙离子(Ca^(2+))胁迫下槲蕨根茎中代谢物的变化,该研究采用超高效液相色谱联用四极杆飞行时间质谱(UPLC-QTOF/MS)技术对不同浓度(0、600、1200 mmol·L^(-1))Ca^(2+)胁迫下槲蕨根茎进行非靶向代谢组学分析。结果表明:(1)共鉴定到64种差异表达代谢物。(2)在0、600 mmol·L^(-1)比较组中有48个差异表达代谢物,在0、1200 mmol·L^(-1)比较组中有45个差异表达代谢物,在600、1200 mmol·L^(-1)比较组中有44个差异表达代谢物。(3)鉴定到的差异表达代谢物根据其化学分类归属信息进行归类,分为5类。综上认为,Ca^(2+)胁迫影响槲蕨根茎的氨基酸代谢、黄酮类化合物生物合成、木质素生物合成、脂肪酸代谢及其他途径;该研究通过非靶向代谢组学分析,初步揭示了参与槲蕨根茎应答Ca^(2+)胁迫的关键代谢物,为进一步研究槲蕨适应Ca^(2+)胁迫的调控机制奠定了基础,也为槲蕨根茎药材的品质改善提供了新思路。

关键词：非靶向代谢组学高浓度Ca^(2+) 代谢通路适应机制苗药

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半夏块茎腐烂病研究进展

热带农业科学 2024年第1期44卷 135-139页

作者：李伟忠曹海禄梁春波檀龙颜贵州中医药大学药学院贵州贵阳550025 中国中药协会中药材种植养殖专业委员会北京100700

半夏块茎腐烂病是半夏生产的主要限制因素之一,严重影响半夏的产量和品质。由于半夏块茎腐烂病的致病菌为土传病原菌,防治尤为困难。文章从半夏块茎腐烂病的致病因子与防治措施、病原菌分离与鉴定、抗病资源筛选、抗病机理研究等方面阐... 详细信息

半夏块茎腐烂病是半夏生产的主要限制因素之一,严重影响半夏的产量和品质。由于半夏块茎腐烂病的致病菌为土传病原菌,防治尤为困难。文章从半夏块茎腐烂病的致病因子与防治措施、病原菌分离与鉴定、抗病资源筛选、抗病机理研究等方面阐述了半夏块茎腐烂病研究进展,为半夏抗腐烂病种质资源挖掘及抗病分子育种等提供理论依据。

关键词：半夏块茎腐烂病致病菌研究进展

基于转录组分析鉴定半夏叶片机械损伤应答基因

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贵州农业科学 2024年第2期52卷 113-123页

作者：陈宏宇李伟忠梁春波梁彦涛檀龙颜于莹贵州中医药大学药学院贵州贵阳550025 贵州中医药大学基础医学院贵州贵阳550025

【目的】探明半夏叶片在机械损伤胁迫应答方面的基因表达差异,为进一步研究半夏胁迫应答机制提供参考。【方法】对半夏在机械损伤胁迫下的叶片进行转录组测序,并比较分析基因表达与调控通路。【结果】从3个组织共获得246711个单基因(Uni... 详细信息

【目的】探明半夏叶片在机械损伤胁迫应答方面的基因表达差异,为进一步研究半夏胁迫应答机制提供参考。【方法】对半夏在机械损伤胁迫下的叶片进行转录组测序,并比较分析基因表达与调控通路。【结果】从3个组织共获得246711个单基因(Unigene),其中117798个基因在Nr、COG等4个数据库中获得注释。剪切型机械损伤和针刺型机械损伤分别识别7895个和22972个应答基因。在应答基因中,发现多个机械损伤胁迫的基因成员。【结论】半夏机械损伤应答过程可能与防御应答、损伤应答和叶绿体类囊体膜等多个过程有关。

关键词：半夏转录组机械损伤基因

Interface failure of segmental tunnel lining strengthened with steel plates based on fracture mechanics

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Frontiers of Structural and Civil Engineering 2024年第1期18卷 137-149页

作者： Yazhen SUN Yang YU Jinchang WANG longyan WANG School of Transportation and Geomatics Engineering Shenyang Jianzhu UniversityShenyang 110168China School of Civil Engineering Shenyang Jianzhu UniversityShenyang 110168China Institute of Transportation Engineering Zhejiang UniversityHangzhou 310058China Center for Balance Architecture Zhejiang UniversityHangzhou310058China

Segmental tunnel lining strengthened with steel plates is widely used worldwide to provide a permanent strengthening method.Most existing studies assume an ideal steel-concrete interface,ignoring discontinuous deformation characteristics,making it difficult to accurately analyze the strengthened structure’s failure mechanism.In this study,interfacial fracture mechanics of composite material was applied to the segmental tunnel lining strengthened with steel plates,and a numerical three-dimensional solid nonlinear model of the lining structure was established,combining the extended finite element method with a cohesive-zone model to account for the discontinuous deformation characteristics of the interface.The results accurately describe the crack propagation process,and are verified by full-scale testing.Next,dynamic simulations based on the calibrated model were conducted to analyze the sliding failure and cracking of the steel-concrete interface.Lastly,detailed location of the interface bonding failure are further verified by model test.The results show that,the cracking failure and bond failure of the interface are the decisive factors determining the instability and failure of the strengthened structure.The proposed numerical analysis is a major step forward in revealing the interface failure mechanism of strengthened composite material structures.

关键词： segmental tunnel lining steel plate strengthening connecting interface cohesive-zone model extended finite element method

Targeting CD47-SIRPα axis for Hodgkin and non-Hodgkin lymphoma immunotherapy

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Genes & Diseases 2024年第1期11卷 205-217页

作者： Pengcheng Zhao longyan Xie Lei Yu Ping Wang School of Life Sciences and Medicine Shandong University of TechnologyZiboShandong 255000China Tongji University Cancer Center Shanghai Tenth People's HospitalSchool of MedicineTongji UniversityShanghai 200092China

The interaction between cluster of differentiation 47(CD47)and signal regulatory proteinα(SiRPa)protects healthy cells from macrophage attack,which is crucial for maintain-ing immune homeostasis.Overexpression of CD47 occurs widely across various tumor cell types and transmits the"don't eat me"signal to macrophages to avoid phagocytosis through binding to SIRPa.Blockade of the CD47-SIRPa axis is therefore a promising approach for cancer treat-ment.Lymphoma is the most common hematological malignancy and is an area of unmet clin-ical need.This review mainly described the current strategies targeting the CD47-SIRPa axis,including antibodies,SiRPaFc fusion proteins,small molecule inhibitors,and peptides both in preclinical studies and clinical trials with Hodgkin lymphoma and non-Hodgkin lymphoma.

关键词： Cancer treatment CD47-SIRPαaxis Hodgkin lymphoma Immunotherapy Non-Hodgkin lymphoma

Physiological and proteomic analysis of salinity tolerance in germinating seeds from Brassica napus

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Physiological and proteomic analysis of salinity tolerance i...

Rab18 maintains homeostasis of subcutaneous adipose tissue to prevent obesity-induced metabolic disorders

生态文明建设中的植物学：现在与未来——中国植物学会第十...

作者： tan longyan Zhao Qi Qiu Lili Dai Shaojun Alkali Soil Natural Environmental Science Center Northeast Forestry University College of Life and Environmental Sciences Shanghai Normal University

Soil salinity is a major abiotic stress that has a serious influence on seed germination.Studying salinity tolerance mechanism during seed germination will provide valuable information for engineering plants to enhance salt tolerance.Oilseed from Brassica species is an important source of vegetable oil,and most of the Brassica species are moderately salt-tolerant.In the present study,the mature and germinating seeds of Brassica napus were investigated under NaCl(0 mM,100 mM,and 200 mM) treatments using physiological and proteomic approaches.The germination rate,biomass,membrane integrity,osmolyte contents,and the activities of antioxidant enzymes in seeds were measured.Using comparative proteomic technology,57 and 46 salt-responsive proteins were identified in mature and germinating seeds under salt conditions,respectively.These proteins were mainly involved in signaling,carbohydrate and energy metabolism,protein metabolism,stress and defense.Our proteomics results suggested both nuclear and mitochondria genes-encoded proteins played important roles in seed germination and salt tolerance.The energy-related metabolism(e.g.TCA cycle,glycolysis,and gluconeogenesis) were enhanced under NaCl stress,which could provide sufficient energy for salt tolerance.Besides,the abundances and activities of antioxidant enzymes were changed in germinating seeds under NaCl stress,implying that diverse ROS scavenging pathways were triggered to cope with salt stress.In addition,our results revealed that alterations in osmotic homoestasis,active protein processing and turnover,and enhanced methionine cycle were necessary for seed germination and salt tolerance.

关键词： Seed germination Brassica napus Proteomics Salinity tolerance

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Science China(Life Sciences) 2024年第6期67卷 1170-1182页

作者： Jiaming Liu Liangkui Li Dijin Xu Yuqi Li Tao Chen Yeyang Liu Yuqian Bao Yan Wang longyan Yang Peng Li Li Xu State Key Laboratory of Membrane Biology and Tsinghua-Peking Center for Life Sciences Beijing Advanced Innovation Center for Structural BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Shanghai Qi Zhi Institute Shanghai 200232China Shanghai Key Laboratory of Metabolic Remodeling and Health Institute of Metabolism and Integrative BiologyFudan UniversityShanghai 200438China Tianjian Laboratory of Advanced Biomedical Sciences Zhengzhou UniversityZhengzhou 450001China Department of Physiology School of Basic Medical SciencesGannan Medical UniversityGanzhou 341000China Department of Endocrinology and Metabolism Shanghai Jiao Tong University School of Medicine Affiliated Sixth People’s HospitalShanghai 200025China Center for Endocrine Metabolism and Immune Diseases Beijing Luhe HospitalCapital Medical UniversityBeijing 101149China Beijing Key Laboratory of Diabetes Research and Care Beijing 101149China

Metabolically healthy obesity refers to obese individuals who do not develop metabolic disorders.These people store fat in subcutaneous adipose tissue(SAT)rather than in visceral adipose tissue(VAT).However,the molecules participating in this specific scenario remain elusive.Rab18,a lipid droplet(LD)-associated protein,mediates the contact between the endoplasmic reticulum(ER)and LDs to facilitate LD growth and maturation.In the present study,we show that the protein level of Rab18 is specifically upregulated in the SAT of obese people and mice.Rab18 adipocyte-specific knockout(Rab18 AKO)mice had a decreased volume ratio of SAT to VAT compared with wildtype mice.When subjected to high-fat diet(HFD),Rab18 AKO mice had increased ER stress and inflammation,reduced adiponectin,and decreased triacylglycerol(TAG)accumulation in SAT.In contrast,TAG accumulation in VAT,brown adipose tissue(BAT)or liver of Rab18AKO mice had a moderate increase without ER stress stimulation.Rab18 AKO mice developed insulin resistance and systematic inflammation.Rab18 AKO mice maintained body temperature in response to acute and chronic cold induction with a thermogenic SAT,similar to the counterpart mice.Furthermore,Rab18-deficient 3T3-L1 adipocytes were more prone to palmitate-induced ER stress,indicating the involvement of Rab18 in alleviating lipid toxicity.Rab18 AKO mice provide a good animal model to investigate metabolic disorders such as impaired SAT.In conclusion,our studies reveal that Rab18 is a key and specific regulator that maintains the proper functions of SAT by alleviating lipid-induced ER stress.

关键词： Rab18 subcutaneous adipose tissue(SAT) metabolically healthy obesity(MHO) lipotoxicity ER stress ectopic lipid storage LD-ER contact

Development of a potent benzonitrile-based inhibitor of glutaminyl-peptide cyclotransferase-like protein(QPCTL)with antitumor efficacy

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Signal Transduction and Targeted Therapy 2024年第1期9卷 44-46页

作者： Lei Yu Pengcheng Zhao Yaoliang Sun Zening Zheng Wenhao Du Lishan Zhang Yaxu Li longyan Xie Shilin Xu Ping Wang Tongji University Cancer Center Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghaiChina Department of Medicinal Chemistry Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghaiChina

Dear Editor,Immune checkpoint therapies manipulating the immune system to eliminate tumor cells have shown remarkable clinical efficacy in treating various cancers.CD47,an emerging efficient immune checkpoint,is crucial for cancer cells to evade macrophagemediated phagocytosis by interaction with signal-regulatory proteinα(SIRPα).Antibodies blocking the CD47/SIRPαinteraction have been effective to promote macrophage-mediated phagocytosis in various types of cancer in mice and humans.CD47 is not only highly expressed in tumor cells,but also normal cells,such as red blood cells(RBCs).Thus,during clinical trials involving cancer patients,anti-CD47 antibodies may promote the macrophagesmediated phagocytosis of RBCs,ultimately inducing undesirable anemia side effects.In contrast,small molecule inhibitors interrupting CD47/SIRPαaxis have shown potential to overcome the anemia,possibly due to their lower immunogenicity and shorter half-life compared to antibodies.1 Hence,developing the novel strategies,especially those without the anemia side effect,to intervene in CD47/SIRPαinteraction will benefit cancer immunotherapy.

关键词： anemia overcome eliminate

朱砂根叶响应Ca^(2+)胁迫的差异代谢物分析

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植物生理学报 2023年第9期59卷 1771-1782页

作者：马洪娜吴依琳马珊珊檀龙颜周英贵州中医药大学药学院贵阳550025

为研究朱砂根叶片对Ca^(2+)胁迫的适应机制,采用非靶向代谢组学技术对不同浓度(0、200、400mmol·L^(-1))Ca^(2+)胁迫5 d的朱砂根叶片进行分析。结果表明,相比对照组,200 mmol·L^(-1)Ca^(2+)胁迫有87个差异代谢物,400 mmol·L^(-1)Ca^(... 详细信息

为研究朱砂根叶片对Ca^(2+)胁迫的适应机制,采用非靶向代谢组学技术对不同浓度(0、200、400mmol·L^(-1))Ca^(2+)胁迫5 d的朱砂根叶片进行分析。结果表明,相比对照组,200 mmol·L^(-1)Ca^(2+)胁迫有87个差异代谢物,400 mmol·L^(-1)Ca^(2+)胁迫有107个差异代谢物,而400和200 mmol·L^(-1)Ca^(2+)胁迫组之间存在89个差异代谢物。这些差异代谢物主要包括氨基酸类、脂肪酸类、苯甲酸类、黄酮类和木质素类代谢物。朱砂根叶片主要通过调节氨基酸类代谢物的含量调节渗透平衡,通过调节多不饱和脂肪酸类代谢物的含量维持细胞膜稳定,通过调节苯甲酸类代谢物、黄酮类代谢物的含量清除活性氧自由基,通过调节木质素类代谢物的含量增强细胞壁厚度等方式适应Ca^(2+)胁迫。

关键词： Ca^(2+)胁迫代谢组学朱砂根适应机制