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Journal of Agricultural Science and Technology(B) 2013年第3期3卷 230-237页

作者： Ronivaldo Rodrigues da Silva tamara Angelo Hamilton Cabral Institute of Biosciences Letters and Exact Sciences Sao Paulo State University UNESP SSo Josd do Rio Preto Sao Paulo Brazil Department Pharmaceutical Sciences School of Pharmaceutical Sciences ofRibeirao Preto University ofSao Paulo RibeiraoPreto Sao Paulo CEP 14040-903 Brazil

In this present work, the best conditions for production of peptidases under solid state fermentation by the fungi Penicillium corylophilum and Penicillium waksmanii, partial purification using Sephadex G-75 gel filtration column, as well as the biochemical characterization of the partial purified enzymes were investigated. P. corylophilum showed the best production in medium containing wheat bran, agro-industrial residue, without additives （egg albumin or casein）, in which peptidase activity reached 520 U mL^-1 and the enzyme displayed the optimum activity between pH range from 7 to 8 and 60 ℃. It also showed high stability in wide pH range and temperature until 45 ℃ for 60 min of incubation. On the other hand, P. waksmanii, the best production was noted in a medium containing wheat bran （95%） and casein （5%）, reaching 545 U mL^-1, with proteolytic optimum activity at pH 7.5 and 55 ℃. The enzyme was mainly stable in pH range from 8 to 9 and at temperatures until 45 ℃ for 60 rain of incubation. The peptidases secreted by both fungi were inhibited in the presence of phenylmethane sulfonyl fluoride, showing that they belong to the subclass of serine peptidases.

关键词： Serine peptidase biochemical characterization enzymatic extract fungi.

Extracavity Parametric Oscillation with Continuous and/or Discontinuous Frequency Tuning

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Optics and Photonics Journal 2012年第2期2卷 119-122页

作者： Valerik S. Ayrapetyan tamara A. Shirokova Siberian State Academy of Geodesy 10 Novosibirsk Russia

A continuously or/and discontinuously tunable LiNbO3 Optical Parametric Oscillator (OPO) with the spectral range of 1.42 - 4.24 um and shifting of 0…12 nm is created and investigated. The OPO resonator ring circuit provides for the radiation energy output value of up to 50 mJ. Radiation bandwidth narrowing of up to 0.7 cm–1 by introducing of the Fabry-Perot etalon into the OPO resonator has been obtained.

关键词： Optical Parametric Oscillator Fabry-Perot Etalon Analog-Digital Converter

The cell phone in the twenty-first century: Risk for addiction or ingestion? Case report and review of the literature

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World Journal of Emergency Medicine 2021年第4期12卷 335-336页

作者： Sanja Dragasevic Milica Stojkovic Lalosevic Ljubisa Toncev tamara Milovanovic Aleksandra Pavlovic Markovic Srdjan Djuranovic Dragan Popovic Clinic for Gastroenterology and Hepatology Clinical Center of SerbiaBelgrade 11000Serbia School of Medicine University of BelgradeBelgrade 11000Serbia

Dear editor,Foreign body ingestion and food bolus impaction are frequent emergency cases in clinical practice.[1] The majority of foreign body ingestion cases occur in the pediatric population, while individuals with mental illness,neurodevelopmental disorders, alcohol intoxication, and prisoners are recognized as a high-risk adult population for foreign body ingestion.

关键词： cases clinical editor

Radiobiogeochemical Assessment of the Soil Near the Issyk-kul Region

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Journal of Geological Resource and Engineering 2016年第1期4卷 39-43页

作者： Djenbaev Bekmamat Kaldybaev Bakit Toktoeva tamara Kenjebaeva Aigul Biology & Pedology Institute of National Academy of Science Chui Ave 265 Bishkek 720071 Kyrgyz Republic

The results of researches are presented the concentration of humus, macro and trace elements and radionuclide in the soil of the Issyk-Kul region. It has given radio biogeochemical assessment of the current status of ... 详细信息

关键词： Soil radionuclide radiation background radioactive tailing Issyk-Kul region

A Quantitative Analysis of Dose Distributions of Two Tangential Whole Breast Irradiation Techniques

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Journal of Physical Science and Application 2016年第5期6卷 27-30页

作者： tamara Jovanovic Jelena Stankovic Dragan Nikolic Jelica Jovev Clinical Center Nis Clinic of Oncology Nis Serbia

We compared DVHs （dose volume histograms） of two tangential irradiation techniques for whole breast RT （radiotherapy） CT （computed tomography） based 2D （two dimensional） technique with wedge filters and 3D CRT （three dimensional conformal radiotherapy） with segmented fields. These two modalities are commonly used in external beam radiotherapy of breast cancer in Clinical Center Nis, Clinic of Oncology, Serbia. Therapy plans that employed two techniques were generated for eighteen patients. Dosimetric outcomes of each technique were investigated. The maximum dose of breast CTV was significantly reduced from 55.83 Gy for 2D to 54.1 Gy for 3D CRT. Also, the maximum dose of lung tissue decreased from 51.81 Gy for 2D to 49.61 Gy for 3D. The dose conformity and uniformity were much better with 3D CRT segmented field technique.

关键词： Breast cancer conformal technique virtual simulation tangential fields segmented fields.

Inhalation treatment of lung cancer: the influence of composition, size and shape of nanocarriers on their lung accumulation and retention

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Cancer Biology & Medicine 2014年第1期11卷 44-55页

作者： Olga B.Garbuzenko Gediminas Mainelis Oleh Taratula tamara Minko Department of Pharmaceutics Ernest Mario School of Pharmacy Rutgers The State University of New Jersey Department of Environmental Sciences Rutgers The State University of New Jersey Environmental and Occupational Health Sciences Institute Department of Pharmaceutical Sciences Oregon State University Rutgers Cancer Institute of New Jersey

Objective： Various nanoparticles have been designed and tested in order to select optimal carriers for the inhalation delivery ofanticancer drugs to the lungs. Methods： q-he following nanocarriers were studied： micelles, liposomes, mesoporous silica nanoparticles （MSNs）, poly propyleneimine （PPI） dendrimer-siRNA complexes nanoparticles, quantum dots （QDs）, and poly （ethylene glycol） polymers. All particles were characterized using the following methods： dynamic light scattering, zeta potential, atomic force microscopy, in vitro cyto- and genotoxicity. In vivo organ distribution of all nanopartides, retention in the lungs, and anticancer effects of liposomes loaded with doxorubicin were examined in nude mice after the pulmonary or intravenous delivery. Results： Significant differences in lung uptake were found after the inhalation delivery of lipid-based and non-lipid-based nanoparticles. The accumulation ofliposomes and miceUes in lungs remained relatively high even 24 h after inhalation when compared with MSNs, Q Ds, and PPI dendrimers. There were notable differences between nanoparticle accumulation in the lungs and other organs 1 and 3 h after inhalation or intravenous administrations, but 24 h after intravenous injection all nanoparticles were mainly accumulated in the liver, kidneys, and spleen. Inhalation delivery of doxorubicin by liposomes significantly enhanced its anticancer effect and prevented severe adverse side effects of the treatment in mice bearing the orthotopic model of lung cancer. Conclusion： The results of the study demonstrate that lipid-based nanocarriers had considerably higher accumulation and longer retention time in the lungs when compared with non-lipid-based carriers after the inhalation delivery. These particles are most suitable for effective inhalation treatment of lung cancer.

关键词： Nanoparticles drug delivery systems intravenous administration lung neoplasms inhalation administration

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农产品可控瞬时压差加工技术研究进展

现代食品科技 2017年第5期33卷 311-318页

作者：易建勇毕金峰彭健 tamara Allaf Karim Allaf 中国农业科学院农产品加工研究所、农业部农产品加工综合性重点实验室北京100193 Laboratory of Engineering Science for Environment LaSIE-UMR-ONRS 7356 University of La RochelleLa Rochelle 17042France ABCAR-DIC Process BP12053 La Rochelle Cedex01 17010France

可控瞬时压差加工技术(Instant controlled pressure drop processing,法语为Détente instantannée contr?lée,简称DIC),又称变温压差膨化技术,于1988年由法国学者发明。DIC加工技术的原理是基于将处理仓内高压(或大气压力)瞬时下降... 详细信息

可控瞬时压差加工技术(Instant controlled pressure drop processing,法语为Détente instantannée contr?lée,简称DIC),又称变温压差膨化技术,于1988年由法国学者发明。DIC加工技术的原理是基于将处理仓内高压(或大气压力)瞬时下降到真空状态,利用这一过程中产生的热力学效应使物料内部水分快速闪蒸,实现体积膨胀或质构改性等目的。经DIC技术处理后的农产品可获得多孔的组织结构和酥脆的口感。此外,DIC技术的优点还体现在色泽、风味,以及较低的生产成本等方面。本文综述了国内外可控瞬时压差加工技术的研究和应用现状,重点介绍了DIC在农产品质构改性、化学成分提取、杀菌消毒、食物脱敏、粮食干燥过程减损、生物能源制造、生物大分子改性和农产品干燥等方面的研究进展,分析了国内可控瞬时压差加工技术存在的主要问题,并对该技术未来的发展趋势进行了展望。

关键词：质构改性提取膨化干燥杀菌瞬时压差

East Java Propolis Inhibits Cytokine Pro-inflammatory in Odontoblast-Like Cells Human Pulp

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Journal of Agricultural Science and Technology(A) 2014年第1期4卷 27-32页

作者： Ira Widjiastuti tamara Yuanita James Hutagalung Mandojo Rukmo Indri Safitri Department of Conservative Dentistry Faculty of Dentistry Airlangga University Surabaya 60132 Indonesia Institute of Tropical Deases Airlangga University Surabaya 60132 Indonesia Department of Biochemistry Faculty of Medicine Airlangga University Surabaya 60132 Indonesia

Inflammation in pulp tissue is caused by caries bacteria. Most bacteria found are Lactobacillus acidophilus. Propolis is a sticky resin material that is derived from the bees and the surrounding plants, which are reported to have several biological effects including anti-bacterial and anti-inflammatory. This study will prove the effectiveness of propolis against proinflammatory cytokines on odontoblast-like cells in human dental pulp. This study was performed on cultured odontoblast-like cells in pulp. Cell culture was derived from the pulp tissue of human M3 teeth that had been extracted. Odontoblast pulp culture was divided in three groups： group one, cultured cells with propolis; group two, cultured cells induced with inactive Lactobacillus acidophilus; group three, cultured cells induced with inactive Lactobacillus acidophilus and exposed to 3 μg/mL propolis. A measurement of TNF-α and TGF-β1 expression was done using the immunocytochemical technique to determine the effectiveness of propolis extracts from East Java Indonesia against proinfalmmatory cytokines. Data were analyzed using the analysis of variance （ANOVA） test （P = 0.05）. Propolis extract can inhibit the expression of TNF-α and increase the expression of TGF-β1 on odontoblast-like cell in human dental pulp. Anti-inflammatory effects of East Java propolis extract are associated with cytokine modulation.

关键词： Propolis extract TNF-α TGF-β1 odontoblast-like cells.

Anti-Aging Effect of <i>Cynara cardunculus</i>L. var. <i>Cynara scolymus</i>L. Extract in D-Galactose-Induced Skin Aging Model in Rats

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Pharmacology & Pharmacy 2018年第10期9卷 428-439页

作者： Galina Sukoyan Edisher Tsivtsivadze Veronika Golovach tamara Kezeli Natalya Demina International Center of Introduction of New Biomedical Technology Tbilisi Georgia Department of Pharmacology Ivane Javakhishvili Tbilisi State University Tbilisi Georgia Department of Pharmacy Technology of Federal State Autonomous Educational Institution of Higher Education I. M. Sechenov of the Ministry of Health of the Russian Federation (Sechenov University) Moscow Russia

The aim of the study was to evaluate the efficacy of 2% Cynara scolymus L. extracts in reverse disturbances of collagen metabolism and inflammation in D-galactose induced skin aging model in rats. D-galactose induced aging reproduced in 48 animals of main group, and 12 rats in control group. All animals in main group were randomized for 4 groups: I. aging + saline, II - IV. different manufacturers 2% artichoke extracts (with content of chlorogenic acid 2.5%) in a dose of intradermal injection 0.13 mg twice at weeks during 4 weeks. Influence of artichoke extracts restored skin relative weight and led to an increase of solubility in neutral salt and acid, and decreased pepsin solubility collagen fraction, restored the hexosamine/collagen (hydroxyproline) ratio and decreased the activity of nuclear transcription factor (NF-kB). Local prolonged treatment with artichoke extracts improved collagen metabolism and attenuated the progression of inflammation in D-galactose induced skin aging model.

关键词： Skin Aging Collagen Pure Water Cynara cardunculus L. var. Cynara scolymus L. Extract Nuclear Transcription Factor Kappa B

Variability of anti-human transglutaminase testing in celiac disease across Mediterranean countries

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World Journal of Gastroenterology 2017年第24期23卷 4437-4443页

作者： Andrea Smarrazzo Giuseppe Magazzù Mongi Ben-Hariz Maria Legarda tamara Virtut Velmishi Elefhteria Roma Aydan Kansu Dusanka Micetic-Turk Enzo Bravi Pio Stellato Carmela Arcidiaco Luigi Greco Department of Translational Medical Sciences School of MedicineSection of PediatricsUniversity of Naples“Federico II” European Laboratory for Food Induced Diseases Celiac Regional Centre Pediatric Gastroenterology and Cystic Fibrosis UnitUniversity of Messina Pediatric Unit Mongi SLIM’s Hospital of Tunis Paediatric Gastroenterology Unit Cruces University Hospital Service of Pediatric Gastroenterology"Mother Teresa"Hospital First Department of Pediatrics University of Athens Department of Pediatric Gastroenterology School of MedicineAnkara University University Medical Centre Paediatric Department Eurospital SpA

To verify the precision and accuracy of transglutaminase antibodies (TGA) assays across Mediterranean countries. METHODSThis study involved 8 referral centres for celiac disease (CD) in 7 Mediterranean countries. A central laboratory prepared 8 kits of 7 blinded and randomized serum samples, with a titrated amount of Human TGA IgA. Each sample was analysed three times on three different days, with each centre running a total of 21 tests. The results were included in a blindly coded report form, which was sent to the coordinator centre. The coordinator estimated the mean coefficient of Variation (CoVar = σ/μ), the mean accuracy (Accur = Vobserved - Vreal) and the mean percent variation (Var% = [(Vobserved - Vreal)/Vreal] × 100). RESULTSThe analysis showed that 79.17% of the mean variation fell between -25% and +25% of the expected value, with the accuracy and precision progressively increasing with higher titres of TGA. From values 1.25 times greater than the normal cut-off, the measurements were highly reliable. CONCLUSIONTGA estimation is a crucial step for the diagnosis of CD; given its accuracy and precision, clinicians could be confident in establishing a diagnosis.

关键词： Coeliac disease Gluten Transglutaminase Non-invasive testing Accuracy Precision