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Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences

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Advances in Microbiology 2014年第13期4卷 857-870页

作者： I-Nung Huang Yuri Sato Mitsuo Sakamoto Moriya Ohkuma Shinobu Ohnuma takeshi naitoh Chikashi Shibata Akira Horii Junko Nishimura Haruki Kitazawa Tadao Saito Graduate School of Agricultural Science Tohoku University Miyagi Japan Japan Collection of Microorganisms RIKEN BioResource Center Ibaraki Japan Department of Surgery Tohoku University Graduate School of Medicine Miyagi Japan Department of Molecular Pathology Tohoku University Graduate School of Medicine Miyagi Japan

Although the gut microflora is thought to be an essential factor in the development of ulcerative colitis (UC), the entire gut microflora occurring in UC remains unknown. Most studies use feces to represent the microflora distribution;however, here we analyzed the bacterial diversity in colonic mucus from UC patients receiving colectomy surgery and control patients. The diversity of microflora was investigated using a combination of terminal restriction fragment length polymorphism (T-RFLP) and clone library analyses of the 16S rRNA gene sequences. In the T-RFLP analysis, the number of terminal restriction fragments (T-RFs) decreased significantly in UC patients when compared to control samples. Also in the clone library analysis, the number of operational taxonomic units (OTU) and the Shannon diversity index were reduced significantly in UC patients. These molecular analyses reveal an overall dysbiosis in UC patients. No specific pathogen was found, and a strong negative correlation in relative abundance of bacterial populations was observed between the phyla Bacteroidetes and Firmicutes in the UC patients. This is the first report showing a significant correlation between these two phyla, which may be important characteristics in the pathogenesis of UC.

关键词： Ulcerative Colitis Microflora Terminal Restriction Fragment Length Polymorphism 16S rRNA Gene Clone Library

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Case of pancreatic metastasis from colon cancer in which cell block using the Trefle^■ endoscopic scraper enables differential diagnosis from pancreatic cancer

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World Journal of Gastrointestinal Oncology 2018年第3期10卷 91-95页

作者： Akihisa Kato Itaru naitoh Hiroyuki Kato Kazuki Hayashi Katsuyuki Miyabe Michihiro Yoshida Yasuki Hori Makoto Natsume Naruomi Jinno takeshi Yanagita Shuji Takiguchi Satoru Takahashi Takashi Joh Department of Gastroenterology and Metabolism Nagoya City University Graduate School of Medical Sciences Department of Experimental Pathology and Tumor Biology Nagoya City University Graduate School of Medical Sciences Department of Gastroenterological Surgery Nagoya City University Graduate School of Medical Sciences

Endoscopic transpapillary brush cytology and forceps biopsy during endoscopic retrograde cholangiopancreatology are generally used to obtain pathological evidence of biliary strictures. Recently, the new endoscopic scraper Trefle~? has been reported and demonstrated high cancer detectability in malignant biliary strictures. This device is used to scrape the stricture over the guidewire, and, in the original method, the tissue and/or cell samples obtained are subjected to histological and/or cytological analysis separately. However, discrimination of chunks of tissue is hampered by the opacity of the surrounding fluid. We have developed a cell block technique for the Trefle~? device without dividing obtained specimens into tissue and cellular components, which is the simplest method and enables immunohistochemical analysis. We present a case of obstructive jaundice diagnosed immunohistochemically as pancreatic metastasis from colon cancer using cell block sections obtained with the Trefle~? device, which procedure is as easy as conventional brush cytology.

关键词： Trefle? Cell block Endoscopic scraper Pancreatic metastasis Biliary strictures

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Spreading pattern and CT findings of very early-stage peripheral small-cell lung cancer

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Advances in Lung Cancer 2013年第4期2卷 73-78页

作者： takeshi Terashima Tatsu Matsuzaki Shotaro Chubachi Fumio Sakamaki Minako Satoh Eri Tomioka George Horio Rika Ogawa Asuka naitoh Hiroya Ojiri Department of Radiology Tokyo Dental College Ichikawa General Hospital Ichikawa Japan Department of Respiratory Medicine Tokyo Dental College Ichikawa General Hospital Ichikawa Japa Department of Respiratory Medicine Tokyo Dental College Ichikawa General Hospital Ichikawa Japan Department of Respiratory Medicine Tokyo Saiseikai Central Hospital Tokyo Japan

Chest CT frequently reveals small pulmonary lesions. If there is no associated hilar or mediastinal lymphadenopathy, small-cell lung cancer (SCLC) is considered to be very early-stage. Few studies, however, have described the characteristic CT findings of very early-stage SCLC. Clarifying these findings would reduce diagnostic delay. The purpose of this study was to determine the characteristic CT findings of very earlystage SCLC. Computed tomography images obtained from 547 consecutive patients with lung cancer between 2003 and 2012 at Tokyo Dental College Ichikawa General Hospital were reviewed retrospectively. One hundred of these patients had SCLC and 447 had non-SCLC (NSCLC). These CT images, along with any that had also been obtained prior to the one on which the final diagnosis was based, were reviewed. In 5 of the cases reviewed, specific findings and a characteristic spreading pattern were identified on CT images that might have allowed a diagnosis of SCLC to have been made sooner. The findings included a sub-pleural, small nodule accompanied by daughter nodules spreading longitudinally along the bronchovascular bundle, no air bronchogram or cavitation, and background emphysematous change. These were not identified on CT images of very earlystage NCSLC, however (n = 22). Awareness of the relevance of these findings would help physicians and radiologists arrive at a differential diagnosis of small pulmonary nodules.

关键词： SCLC Small Pulmonary Nodules Beaded Appearance Bronchovascular Bundle CT Scan

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