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Feasibility of the Routine Clinical Use of a Multiplex Virus Polymerase Chain Reaction Assay Based on Blood Virus Detection in Hematopoietic Stem Cell-Transplanted Patients

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International Journal of Clinical Medicine 2022年第2期13卷 67-81页

作者： Hiroko Tsunemine Miho Sasaki Yuriko Zushi Toshiharu Saitoh Norio Shimizu Yasuhiro Tomaru Yumi Aoyama Ryusuke Yamamoto Tomomi Sakai Nobuyoshi Arima Taiichi Kodaka takayuki Takahashi Departments of Hematology Shinko Hospital Kobe Japan Cell Therapy Shinko Hospital Kobe Japan Center for Stem Cell and Regenerative Medicine Tokyo Medical and Dental University Tokyo Japan Akasaka Hematology & Oncology Clinic Kobe Japan

Background: Multiplex virus assays are useful in immunocompromised hosts but still challenging in routine clinical settings in terms of their sensitivity, specificity, reproducibility, and time and cost performances. In recent years, we developed a qualitative multiplex virus PCR assay capable of the simultaneous detection of 13 virus species within 3 h. However, because of the multiple and concomitant nature of this virus assay, it should be validated for qualitative reliability. Materials and Methods: As a preclinical examination, this multiplex PCR was able to detect 1.25 × 103 copies/mL of 13 synthesized virus genomes and preserved same virus DNAs by the serial dilution method. Blood samples from 40 patients who underwent hematopoietic stem cell transplantation were then examined by multiplex PCR for 13 virus species, followed by quantitative real-time PCR for all 13 virus species as reference PCR when these patients developed symptoms suggestive of viral infection. Results: In 421 cumulative qualitative-quantitative tests, the multiplex PCR certainly detected 1.0 × 103 copies/mL of 5 viruses (CMV, JCV, BKV, HHV-6, ADV) that were frequently detected and thus reasonably analyzed. The positive and negative predictive values of multiplex PCR were 84.2% - 93.3% and 90.7% - 99.0%, respectively, and sensitivity and specificity were 59.0% - 83.3% and 97.2% - 99.2%, respectively, for these 5 viruses. Conclusion: From these performances, the multiplex PCR assay may be acceptable in a routine clinical laboratory setting.

关键词： Multiplex Virus PCR Assay Routine Laboratory Use Positive Predictive Value Negative Predictive Value Sensitivity Specificity

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LXR, PPAR<i>γ</i>, and PPAR<i>δ</i>Agonists Are Not Sufficient to Demonstrate Therapeutic Potential against Mouse Model of Systemic Lupus Erythematosus

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Open Journal of Rheumatology and Autoimmune Diseases 2017年第2期7卷 128-136页

作者： Noriko Toyota Tatebe Katsue Sunahori Watanabe Sonia Zeggar Sumie Hiramatsu Minglu Yan takayuki Katsuyama Eri Katsuyama Haruki Watanabe Ken-ei Sada Jun Wada Department of Nephrology Rheumatology Endocrinology and Metabolism Okayama University Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama Japan

Aim: We aimed to investigate whether the agonists for liver X receptor (LXR) ameliorate lupus-like phenotypes in mice mediated by the clearance of apoptotic cells, and compare with peroxisome proliferator-activated receptor (PPAR) γ plus PPARδ agonists, which also facilitate the clearance of apoptotic cells and exert anti-inflammatory effects in systemic lupus erythematosus (SLE). Methods: We investigated the efficacy of LXR agonist (GW3965) or dual treatment of PPARγ (pioglitazone) and PPARδ (GW0742) agonists in SLE animal models, female MRL/MpJ-Fas/J mice and BALB/cAJcl mice treated with pristane. The data were analyzed with one-way analysis of variance and Tukey’s honestly significant difference tests. Results: The treatment with LXR or PPARγ/δ agonists did not significantly alter the swelling of lymph nodes, ds-DNA production, albuminuria, histological score of glomerular lesions, and mRNA expression of target genes including Abca1, C1qa, Icam1, Mertk and Tnf. Conclusion: LXR or PPARγ/δ agonists targeting the impaired clearance for apoptosis cells may not be efficient in the remission induction therapy in SLE.

关键词： Nuclear Receptors Liver X Receptor (LXR) Peroxisome Proliferator-Activated Receptor (PPAR) Systemic Lupus Erythematosus (SLE)

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无图形损伤的选择性去除微小尘粒的湿法清洗技术(英文)

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电子工业专用设备 2004年第11期33卷 17-22页

作者： Takehiko Orii takayuki Toshima Kenji Sekiguchi Glenn W.Gale Tokyo Electron AT Limited Nirasaki CityYamanashi 407-0192JAPANTokyo Electron AT LimitedNirasaki CityYamanashi 407-0192JAPAN Tokyo Electron AT LimitedNirasaki CityYamanashi 407-0192JAPAN Tokyo Electron LimitedTBS Broadcast CenterTokyo107-8481JAPAN

The drastic shrinking of semiconductor linewidths has led to a need for new wafer cleaning strategies in the *** past technology generations,particle removal has been accomplished typical-ly using the SC1(NH4OH?H2O2?H2O)solution in conjunction with megasonic energy in a wet bench,or by DI water with a high pressure jet or megasonic nozzle in a wafer ***,such techniques have been found to damage patterns,particularly narrow polysilicon gate ***,the etching of oxide associated with SC1,which facilitates particle removal by an undercutting mechanism,has re cently become a concern due to increas-ingly strict oxide *** alternative technique using an atomized liquid spray nozzle on a single wafer platform is *** droplets are able to effect particle removal without damage to sensitive patterns,by control of their carrier gas(N2)flow *** for particle removal will be *** using this technique show particle removal comparable to megasonics can be ***-more,90nm and65nm polysilicon gate structures were processed with high particle removal efficiency and no damage,as were Al *** of the N2flow rate on particle removal and damage performance will be *** physical mechanism of atomized spray enables chemical etching of oxides to be minimized without sacrificing particle removal *** using this technique in conjunction with chemistry,to further improve particle removal,will be *** over a large range of parti-cle sizes will be *** applications will be discussed.

关键词：尘粒湿法清洗去除选择性 H2O2 OH DI 图形

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EGFR mutation identifies distant squamous cell carcinoma as metastasis from lung adenocarcinoma

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World Journal of Respirology 2013年第2期3卷 38-43页

作者： Nobuhiro Kanaji Shuji Bandoh Toshitetsu Hayashi Reiji Haba Naoki Watanabe Tomoya Ishii Asako Kunitomo takayuki Takahama Akira Tadokoro Osamu Imataki Hiroaki Dobashi Takuya Matsunaga Division of Endocrinology and Metabolism Hematology Rheumatology and Respiratory Medicine Department of Internal Medicine Faculty of Medicine Kagawa University Kagawa 761-0793 Japan Department of Diagnostic Pathology Faculty of Medicine Kagawa University Kagawa 761-0793 Japan

Lung cancer metastasis is typically determined by histologic similarity between distant and primary lesions. Herein, we present a 70-year-old Japanese woman with an adenocarcinoma in her lung and a squamous cell carcinoma in her femur; both tumors had an identical epidermal growth factor receptor mutation, G719 S. This indicated that both tumors had a common origin, despite their histologic dissimilarity. The tumor in the femur was thus identified genetically as a lung cancer metastasis. This case suggests that genetic analysis can determine whether a distant lesion is a lung cancermetastasis, particularly when the histology differs from that of the primary lesion.

关键词： Non-small cell lung cancer Adenocarcinoma Squamous cell carcinoma Epidermal growth factor receptor mutation G719 Metastasis

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轻型纸的制备及其纤维间结合力分析

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中国造纸学报 2012年第4期27卷 10-13页

作者：金海兰岡山隆之于钢东北林业大学生物质材料科学与技术教育部重点实验室黑龙江哈尔滨150040 东京农工大学大学院连合农学研究科日本东京1838509

通过添加松厚剂制备轻型纸,并采用Page理论,从单纤维强度与纤维间结合力方面对轻型纸强度进行了探讨。结果表明,添加松厚剂对轻型纸零距抗张强度的影响很小,但会显著降低纤维间结合力,表明轻型纸强度的降低主要是由纤维间结合力的减弱... 详细信息

通过添加松厚剂制备轻型纸,并采用Page理论,从单纤维强度与纤维间结合力方面对轻型纸强度进行了探讨。结果表明,添加松厚剂对轻型纸零距抗张强度的影响很小,但会显著降低纤维间结合力,表明轻型纸强度的降低主要是由纤维间结合力的减弱引起的;轻型纸光散射系数随纸张强度的降低而提高,这与Page理论的分析相一致;通过扫描电子显微镜观察轻型纸断面发现,添加松厚剂可使纸张厚度明显增大。

关键词：轻型纸抗张强度 Page理论纤维间结合力

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具有优化沟槽HiGT结构的3.3kV/1800 A IGBT及其模块优化设计

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大功率变流技术 2015年第2期 52-56页

作者： takayuki Kushima Katsunori Azuma Yasuhiro Nemoto Katsua kiSaito Yoshihiko Koike 日立功率半导体器件株式会社茨城日立3191221

日立公司开发了具有最高电流等级的3.3k V/1800A IGBT模块,其额定电流等级比现有同尺寸、同电压等级常规产品提高了20%。通过采用优化沟槽Hi GT结构,降低了器件功耗,同时优化了模块的电、热性能设计,降低了热阻以及寄生电感。

关键词： 3-3 kV/1 800AIGBT 优化沟槽HiGT 准交错布局杂散电感寄生电感电极结构

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心肌灌注显像中左心室大小对舒张期功能参数准确性的影响:与组织多普勒超声心动图比较

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中华核医学与分子影像杂志 2017年第6期37卷 383-384页

作者：唐军 Satoshi Kurisu Yoji Sumimoto Hiroki Ikenaga Noriaki Watanabe Ken Ishibashi Yoshihiro Dohi takayuki Hidaka Yukihiro Fukuda Yasuki Kihara 苏州大学附属第二医院核医学科 Department of Cardiovascular Medicine Hiroshima University Graduate School of Biomedical and Health Sciences

目的左心室舒张期功能受损是心肌缺血早期的灵敏征兆。本研究探讨了左心室大小对SPECT心肌灌注显像计算舒张期功能参数准确性的影响。方法研究包括151例已知或可疑冠心病患者,均行SPECT心肌灌注显像和经胸壁超声心动图检查。通过定量门... 详细信息

目的左心室舒张期功能受损是心肌缺血早期的灵敏征兆。本研究探讨了左心室大小对SPECT心肌灌注显像计算舒张期功能参数准确性的影响。方法研究包括151例已知或可疑冠心病患者,均行SPECT心肌灌注显像和经胸壁超声心动图检查。通过定量门控SPECT计算高峰充盈率（PFR）、1/3平均充盈率（1/3 MFR）和高峰充盈时间与RR间期时间的比值（TPFR/RR）。将高峰早期二尖瓣环隙流速（e′）作为左心室舒张期功能的参考标准。结果左室收缩末期容积（ESV）≤10 ml者43例,ESV 11;0 ml者43例,ESV>20 ml者65例;3组患者的PFR（P<0.001）、1/3 MFR（P<0.001）和TPFR/RR（P=0.01）差异均有统计学意义;上述舒张期功能参数随不同患者左心室变小而升高。对所有患者而言,PFR（r=0.24,P=0.003）和1/3 MFR（r=0.31,P<0.001）与左心室大小呈正相关,而TPFR/RR（r=-0.23,P=0.004）和e′呈负相关。多元线性回归分析结果表明,男性（β=-0.14,P=0.07;β=-0.16,P=0.04）、ESV（β=-0.63,P<0.001;β=-0.45,P<0.001）和e′（β=0.36,P<0.001;β=0.40,P<0.001）是PFR和1/3 MFR的相关因素;ESV（β=-0.17,P=0.03）和e′（β=-0.21,P=0.01）是TPFR/RR的相关因素。结论 SPECT计算的PFR、1/3 MFR和TPFR/RR与左心室舒张期功能的参考标准e′有关,但对于心脏较小的患者,这些参数会被高估;解读左心室舒张期功能时要考虑左心室大小。

关键词：左心室舒张期功能舒张期参数小心脏 SPECT

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Comparison of peg-interferon, ribavirin plus telaprevir vs simeprevir by propensity score matching

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World Journal of Hepatology 2015年第28期7卷 2841-2848页

作者： Hideki Fujii Takeshi Nishimura Atsushi Umemura Taichiro Nishikawa Kanji Yamaguchi Michihisa Moriguchi Yoshio Sumida Hironori Mitsuyoshi Chihiro Yokomizo Saiyu Tanaka Hiroki Ishikawa Kenichi Nishioji Hiroyuki Kimura Shiro Takami Yasuyuki Nagao takayuki Takeuchi Toshihide Shima Yoshihiko Sawa Masahito Minami Kohichiroh Yasui Yoshito Itoh Department of Molecular Gastroenterology and Hepatology Kyoto Prefectural University of Medicine Department of Gastroenterology Japanese Red Cross Kyoto Daiichi Hospital Department of Gastroenterology and Hepatology North Medical Center Kyoto Prefectural University of Medicine Department of Gastroenterology Osaka General Hospital of West Japan Railway Company Center for Digestive and Liver Diseases Nara City Hospital Department of Gastroenterology and Hepatology Omihachiman Community Medical Center Health Care Division Kyoto Second Red Cross Hospital Department of Gastroenterology Otsu Municipal Hospital Department of Gastroenterology Matsushita Memorial Hospital Higashiomicity Notogawa Hospital Center of Gastroenterology and Hepatology Saiseikai Suita Hospital Department of Internal Medicine Aiseikai Yamashina Hospital

AIM: To compare efficacy of telaprevir(TVR) and simeprevir(SMV) combined with pegylated interferon(PEG-IFN) and ribavirin(RBV) while treating chronic hepatitis C(CHC). METHODS: In all, 306 CHC patients were included in this study. There were 159 patients in the TVR combination therapy group and 147 patients in the SMV combination therapy group. To evaluate pretreatment factors contributing to sustained virological response at 12 wk(SVR12), univariate and multivariate analyses were performed in TVR and SMV groups. To adjust for patient background between TVR and SMV groups, propensity score matching was performed. Virological response during treatment and SVR12 were ***: Overall rates of SVR12 [undetectable serum hepatitis C virus(HCV) RNA levels] were 79.2% and 69.4% in TVR and SMV groups, respectively. Patients in the SMV group were older, had higher serum HCV RNA levels, lower hemoglobin, higher prevalence of unfavorable interleukin-28B(IL28B) genotype(rs8099917), and poorer response to previous PEG-IFN and RBV treatment. Propensity score matching was performed to adjust for backgrounds(n = 104) and demonstrated SVR12 rates of 74.0% and 73.1% in the TVR and SMV groups, respectively. In the TVR group, discontinuation rates were higher because of adverse events; however, breakthrough and nonresponse was more frequent in the in SMV group. Multivariate analysis revealed IL28 B genotype(rs8099917) as the only independent predictive factor of SVR12 in both ***: SVR12 rates were almost identical following propensity score matching.

关键词： Chronic hepatitis C Combination therapy Pegylated interferon Simeprevir Telaprevir Propensityscore matching Protease inhibitor

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Current perspectives of viral hepatitis

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World Journal of Gastroenterology 2024年第18期30卷 2402-2417页

作者： Daisuke Usuda Yuki Kaneoka Rikuo Ono Masashi Kato Yuto Sugawara Runa Shimizu Tomotari Inami Eri Nakajima Shiho Tsuge Riki Sakurai Kenji Kawai Shun Matsubara Risa Tanaka Makoto Suzuki Shintaro Shimozawa Yuta Hotchi Ippei Osugi Risa Katou Sakurako Ito Kentaro Mishima Akihiko Kondo Keiko Mizuno Hiroki Takami takayuki Komatsu Tomohisa Nomura Manabu Sugita Department of Emergency and Critical Care Medicine Juntendo University Nerima HospitalNerima 177-8521TokyoJapan Department of Sports Medicine Faculty of MedicineJuntendo UniversityBunkyo 113-8421TokyoJapan

Viral hepatitis represents a major danger to public health,and is a globally leading cause of *** five liver-specific viruses:Hepatitis A virus,hepatitis B virus,hepatitis C virus,hepatitis D virus,and hepatitis E virus,each have their own unique epidemiology,structural biology,transmission,endemic patterns,risk of liver complications,and response to antiviral *** remain few options for treatment,in spite of the increasing prevalence of viral-hepatitiscaused liver ***,chronic viral hepatitis is a leading worldwide cause of both liver-related morbidity and mortality,even though effective treatments are available that could reduce or prevent most patients’*** 2016,the World Health Organization released its plan to eliminate viral hepatitis as a public health threat by the year 2030,along with a discussion of current gaps and prospects for both regional and global eradication of viral ***,treatment is sufficiently able to prevent the disease from reaching advanced ***,future therapies must be extremely safe,and should ideally limit the period of treatment necessary.A better understanding of pathogenesis will prove beneficial in the development of potential treatment strategies targeting infections by viral *** review aims to summarize the current state of knowledge on each type of viral hepatitis,together with major innovations.

关键词： Hepatitis A virus Hepatitis B virus Hepatitis C virus Hepatitis D virus Hepatitis E virus Current perspectives

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利用动态子结构系统和MDOF接触网模型对受电弓/接触网系统进行混合仿真测试

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国外铁道车辆 2021年第4期58卷 32-37页

作者： [日]Shigeyuki KOBAYASHI [日]Yoshitaka YAMASHITA [日]takayuki USUDA 岳枰屹(译) 刘宏友(校) 不详

本文基于动态子结构系统(DSS),提出一种受电弓/接触网系统混合仿真(HS)法。与逆传递函数法等常用方法相比,DSS能够更好地避免作动器动力学不稳定性,能更精确地描述受电弓与接触网之间的动力学响应。

关键词：受电弓/接触网系统动态子结构混合仿真日本

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