检索结果-南通市图书馆

Specialized Metabolites of the Flavonol Class Mediate Root Phototropism and Growth

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2016年第12期9卷 1554-1555页

作者： takayuki tohge, Alisdair R. Fernie Max-Planck-Institute of Molecular Plant Physiology Am Mühlenberg 1 14476 Potsdam-Golm Germany

Roots are subterranean organs and normally follow the gravity vector to grow downward. However, in nature, roots can be exposed to sunlight in a negative phototropism response termed root light avoidance （Kutschera and Briggs, 2012）. Such tropic responses are characterized by organ curvature caused by asymmetric growth between different sides of the root, which generally correlates with an asymmetric distribution of the plant hormone auxin （Wang et al., 2015）.

关键词： Conserved Interaction Recruiting

Metabolomics-assisted refinement of the pathways of steroidal glycoalkaloid biosynthesis in the tomato clade

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2014年第9期56卷 864-875页

作者： Kevin Schwahn Leonardo Perez de Souza Alisdair R.Fernie takayuki tohge University of Potsdam AG BioinformaticsInstitute for Biochemistry and Biology MaxPlanck-Institute of Molecular Plant Physiology

Steroidal glycoalkaloids（SGAs） are nitrogen-containing secondary metabolites of the Solanum species,which are known to have large chemical and bioactive diversity in *** recent effort and development on LC/MS techniques for SGA profiling have elucidated the main pathways of SGA metabolism in tomato,the problem of peak annotation still remains due to the vast diversity of chemical structure and similar on overlapping of chemical *** we provide a case study of peak classification and annotation approach by integration of species and tissue specificities of SGA accumulation for provision of comprehensive pathways of SGA *** order to elucidate natural diversity of SGA biosynthesis,a total of 169 putative SGAs found in eight tomato accessions（Soianum lycopersicum, ***, ***, ***, ***,***,***,***） and four tissue types were used for correlation *** results obtained in this study contribute annotation and classification of SGAs as well as detecting putative novel biosynthetic branch *** such this represents a novel strategy for peak annotation for plant secondary metabolites.

关键词： Fruit ripening glycoalkaloids secondary metabolite Solanum lycopersicum tomato accessions

Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2014年第9期56卷 941-959页

作者： Alexandra Florian Stefan Timm Zoran Nikoloski takayuki tohge Hermann Bauwe Wagner L.Araujo Alisdair R.Fernie Max Planck Institute for Molecular Plant Physiology Department of Plant Physiology University of Rostock Max-Planck Partner group at the Departament of Plant Biology Federal University of Vicosa

As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the *** the indiscriminant nature of Rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas ***,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in ***,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains *** address these questions,plants Arabidopsis thaliana（Arabidopsis） ecotype Columbia（Col-O） grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this *** results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite *** results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription.

关键词： Carbon dioxide partial pressures gene expression metabolic profiling oxygen partial pressures

Photorespiration Is Crucial for Dynamic Response of Photosynthetic Metabolism and Stomatal Movement to Altered C02 Availability

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2017年第1期10卷 47-61页

作者： Marion Eisenhut Andrea Brautigam Stefan Timm Alexandra Florian takayuki tohge Alisdair R. Fernie Hermann Bauwe Andreas P.M. Weber Institute of Plant Biochemistry Cluster of Excellence on Plant Sciences Heinrich Heine University 40225 Dusseldorf Germany Department of Plant Physiology University of Rostock Albert-Einstein-StraBe 3 18051 Rostock Germany Department of Molecular Physiology Max-Planck Institute for Molecular Plant Physiology 14476 Potsdam-Golm Germany Present address: IPK Gatersleben Network Analysis and Modeling Corrensstrasse 3 06466 Seeland Germany

The photorespiratory pathway or photorespiration is an essential process in oxygenic photosynthetic or- ganisms, which can reduce the efficiency of photosynthetic carbon assimilation and is hence frequently considered as a wasteful process. By comparing the response of the wild-type plants and mutants impaired in photorespiration to a shift in ambient C02 concentrations, we demonstrate that photorespiration also plays a beneficial role during short-term acclimation to reduced C02 availability. The wild-type plants re- sponded with few differentially expressed genes, mostly involved in drought stress, which is likely a conse- quence of enhanced opening of stomata and concomitant water loss upon a shift toward low C02. In contrast, mutants with impaired activity of photorespiratory enzymes were highly stressed and not able to adjust stomatal conductance to reduced external C02 availability. The transcriptional response of mutant plants was congruent, indicating a general reprogramming to deal with the consequences of reduced C02 availability, signaled by enhanced oxygenation of ribulose-l,5-bisphosphate and amplified by the artificially impaired photorespiratory metabolism. Central in this reprogramming was the pro- nounced reallocation of resources from growth processes to stress responses. Taken together, our results indicate that unrestricted photorespiratory metabolism is a prerequisite for rapid physiological acclimation to a reduction in C02 availability.

关键词： photorespiration starvation stress stomata transcription Arabidopsis thaliana

The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2017年第9期59卷 612-628页

作者： Xiaolei Zhu Jing Yu Jianxin Shi takayuki tohge Alisdair R.Fernie Sagit Meir Asaph Aharoni Dawei Xu Dabing Zhang Wanqi Liang Joint International Research Laboratory of Metabolic&Developmental Sciences Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and HealthSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China Central Metabolism Group Max Planck Institute of Molecular Plant Physiology14476 Potsdam-GolmGermany Department of Plant Sciences Weizmann Institute of SciencePO Box 26Rehovot 76100Israel School of Agriculture Food and WineUniversity of AdelaideWaite CampusSA 5005Australia

Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases （PKSs） are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase （OsPKS2） in male reproduction of rice （Oryza sativa）. Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl- CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT （wild type）, ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds. However, unlike pksb/laps, in which most detected phenolics were substantially decreased, ospks2 accumu- lated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/laps, we propose that PKS proteins have functionally diversified during evolution. Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants.

关键词： PKS The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice Figure

Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Horticulture Research 2019年第1期6卷 556-567页

作者： Wasiye F.Beshir takayuki tohge Mutsumi Watanabe Maarten L.A.TMHertog Rainer Hoefgen Alisdair R.Fernie Bart M.Nicolaï Division of Mechatronics Biostatistics and Sensors(MeBioS)Department of Biosystems(BIOSYST)KU LeuvenLeuvenBelgium Max Planck Institute of Molecular Plant Physiology(MPI-MP) Potsdam-GolmGermany Flanders Centre of Postharvest Technology(VCBT) LeuvenBelgium

In developing apple fruit,metabolic compartmentation is poorly understood due to the lack of experimental *** subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the *** work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit,covering the major events occurring during fruit development(cell division,cell expansion,and maturation).Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments(cytosol,plastids,and vacuole).Evidence is presented that most of the sugars and organic acids were predominantly located in the vacuole,whereas some of the amino acids were distributed between the cytosol and the *** results showed a shift in the plastid marker from the lightest fractions in the early growth stage to the dense fractions in the later fruit growth *** implies that the accumulation of starch content with progressing fruit development substantially influenced the distribution of plastidial fragments within the non-aqueous density gradient *** from this study provide substantial baseline information on assessing the subcellular compartmentation of metabolites in apple fruit in general and during fruit growth in particular.

关键词： technique distribution sugar

Mapping theArabidopsis Metabolic Landscape by Untargeted Metabolomics at Different Environmental Conditions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2018年第1期11卷 118-134页

作者： Si Wu takayuki tohge Alvaro Cuadros-lnostroza Hao Tong Hezi Tenenboim Rik Kooke Michael Meret Joost B. Keurentjes Zoran Nikoloski Alisdair R. Fernie Lothar Willmitzer Yariv Brotman 1 Max Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany MetaSysX GmbH Am Muhlenberg 11 14476 Potsdam-Golm Germany Laboratory of Genetics Wageningen University Droevendaalsesteeg 1 6708 PB Wageningen the Netherlands Department of Life Sciences Ben Gurion University of the Negev Beersheva Israel

Metabolic genome-wide association studies （mGWAS）, whereupon metabolite levels are regarded as traits, can help unravel the genetic basis of metabolic networks. A total of 309Arabidopsis accessions were grown under two independent environmental conditions （control and stress） and subjected to untargeted LC-MS- based metabolomic profiling; levels of the obtained hydrophilic metabolites were used in GWAS. Our two- condition-based GWAS for more than 3000 semi-polar metabolites resulted in the detection of 123 highly resolved metabolite quantitative trait loci （p ≤ 1.0E-08）, 24.39% of which were environment-specific. Interestingly, differently from natural variation in Arabidopsis primary metabolites, which tends to be controlled by a large number of small-effect loci, we found several major large-effect loci alongside a vast number of small-effect loci controlling variation of secondary metabolites. The two-condition-based GWAS was fol- lowed by integration with network-derived metabolite-transcript correlations using a time-course stress experiment. Through this integrative approach, we selected 70 key candidate associations between struc- tural genes and metabolites, and experimentally validated eight novel associations, two of them showing differential genetic regulation in the two environments studied. We demonstrate the power of combining large-scale untargeted metabolomics-based GWAS with time-course-derived networks both performed under different ablotic environments for identifying metabollte-gene associations, providing novel global insights into the metabolic landscape of Arabidopsis.

关键词： untargeted metabolomics GWAS network analysis different environments secondary metabolism

Analysis of the Interface between Primary and Secondary Metabolism in Catharanthus roseus Cell Cultures Using ^13C-Stable Isotope Feeding and Coupled Mass Spectrometry

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2013年第2期6卷 581-584页

作者： Carla Antonio Natali R. Mustafa Sonia Osorio takayuki tohge Patrick Giavalbco Lothar Willmitzer Heiko Rischer Kirsi-Marj Oksman-Caldentey Robert Verpoorte Alisdair R. Fernie Central Metabolism Group Max Planck Institute of Molecular Plant Physiology Am Muehlenberg 1 D-14476 Potsdam-Golm Germany Natural Products Laboratory Institute of Biology Leiden University Einsteinweg 55 2300 RA Leiden The Netherlands Present address: Departamento de Biologia Molecular y Bioquimica Universidad de M.~laga 29071 M=~laga Spain Genes and Small Molecules Group Max Planck Institute of MolecularPlant Physiology Am Muehlenberg 1 D-14476 Potsdam-Golm Germany VTT Technical Research Centre of Finland PO Box 1000 F1-02044 VTT (Espoo) Finland

Dear Editor, The tropical plant Madagascar periwinkle Catharanthus roseus （L.） *** is a rich source of plant-derived medicinal ter-penoid indole alkaloids （TIAs）, including the anti-hypertensive ajmalicine, the sedative compound serpentine, and the anti-cancer drugs vinblastine and vincristine. However, the latter two compounds are produced in C. roseus plants only in very low amounts. Elicitors such as hormones （e.g. jasmonates or salicylic acid） activate plant natural defense responses, includ-ing increased secondary metabolite production （EI-Sayed and Verpoorte, 2007; Lackman et al.. 2011）.

关键词： Secondary Metabolism Terpenes jasmonate biosynthesis Pathway Salicylic Acid cultured cells Interfaces Cisplatin/Etoposide/Methotrexate Catharanthus roseus Interface

Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2020年第7期13卷 1027-1046页

作者： takayuki tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo Mutsumi Watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie Max-Planck-lnstitut fiir Molekulare Pflanzenphysiologie Am Muehlenberg 114476 Potsdam-Golm.Germany Graduate School of Biological Science Nara Institute of Science and TechnologyIkoma 630-0192 Japan Council for Agricultural Research and Economics(CREA) Research Centre for Genomics and Bioinformaticsvia Ardeatina 54600178 RomeItaly Universite de Toulouse INP-ENSA ToulouseGenomique et Biotechnologie des FruitsCastanet-Tolosan 31326France Institute of Plant Systems Biology 4000 PlovdivBulgaria Max Planck Institute for Biology of Ageing Joseph Stelzmann Strasse 9b50931 CologneGermany Institute of Botany and Molecular Genetics BioSCRWTH Aachen University52056 AachenGermany National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China

While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across *** this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of *** this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic *** combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid *** combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit *** results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.

关键词： Solanumly copersicum secondary metabolism natural diversity wild accessions pathway elucidation gene discovery