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Pathogenesis of Demyelinating Encephalopathy in Dogs with Spontaneous Acute Canine Distemper

Journal of Integrative Agriculture 2013年第2期12卷 334-343页

作者： PAN Yao-qian LIU Xing-you MENG Li-ping ZHU Guang-rui XIA Yin-ke CHEN Jin-shan yoshikawa takashi Department of Veterinary Pathology College of Animal Sciences Henan Institute of Science and Technology Department of Microbiology and Immunology Northwestern University Feinberg Medical School Department of Veterinary Pathology School of Veterinary Medicine and Animal Science Kitasato University

So far, the pathogenesis of demyelination caused by canine distemper virus （CDV） in the central nervous system has remained unclear, although a lot of studies have been done extensively. To further investigate the relation of variety cells in brain to demyelination, this study was performed on 15 dogs with spontaneous acute canine distemper and 2 controls. According to anatomical relation, the brain was divided into cerebrum, cerebral stem and cerebellum. The sections with no, mild, moderate, or severe demyelinating lesions were selected respectively and stained by HE and immunohistochemistry. Immuno-localisation of CDV antigen was used to conftrm CDV infection. The brain was examined for co-localisation of the CDV antigen with either an astrocyte-specific marker, glial fibrillary acidic protein （GFAP）, or an oligodendrocyte-specific marker, galactocerebroside （GalC）. Apoptotic cell was detected by TdT-mediated nick end-labeling assay （TUNEL）. The results demonstrated that the local disturbance of blood circulation mainly included congestion, edema, thrombosis, and disseminated intravascular coagulation （DIC）. The CDV neucleocapsid protein positive reaction, metabolic disorder and apoptosis of oligodendrocytes were observed in demyelinating areas. Lots of astrocytes displayed CDV antigen-positive, especially in their process. Some of them became apoptotic cell confirmed by TUNEL staining. Fibrous astrocytes showed more intense GFAP-positive in mild and moderate demyelinating area. Some of nervous cells located in pyramidal cell layers and nucleus nervi were in degeneration, necrosis. Satellitosis, neuronophagia and apoptotic neurons were examined by hematoxylin and eosin （HE） and TUNEL staining. The results suggested that the demyelinating changes in brain tissues infected with CDV mainly related to the metabolic disorder and apoptosis of ogliodendrocytes and astrocytes; also involved with the local disturbance of blood circulation and some neuron lost.

关键词： acute canine distemper demyelinating encephalopathy oligodendrocyte astrocyte apoptosis

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Histopathogenesis of demyelinating encephalopathy on dogs with natural canine distemper

Histopathogenesis of demyelinating encephalopathy on dogs wi...

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中国畜牧兽医学会兽医病理学分第12次暨中国动物病理生理学专业委员会第11次学术讨论会

作者： takashi yoshikawa Department Veterinary Pathology School of Veteribary Medicine and Animal Scienceskitasato University35-1 Higashi-23 banchoTowada cityAomori 034- 8628Japan

Up to now, the pathogenesis of dimyelination in brain tissues has remained unclear, although a lot of studies about neuron and glia changes have been done extensively. To further investigate the relation of variety cell in brain tissue to demyelination, this experiment was performed on 12 dogs with canine distemper. To detect the lesions carefully the brain tissue was divided into three portions and eleven slices that were including cerebrum (four slices) , cerebral stem (five slices) and cerebellum (two slices) according to anatomical relation. The sections stained by HE were observed under light microscope. The sections (no, mild, moderate and severe demyelinatig tissues) were selected respectively and stained by immunohistochemistry. The results demonstrated that the disturbance of blood circulation mainly included congestion, edema, thrombus formation, and the occurrence of diffusive intravascular coagulation (DIC). Oli-godendrocytes were occurred metabolic disorders and apoptosis in demyelinating areas. With anti - CDV antigen staining astrocytes were often displayed as intense positive reaction. With anti - GFAP staining, the fibrous astrocytes were more intense positive reaction in acute, mild and moderate demyelinating area, apoptotic astrocytes were detected in lesion foci . Some ependymal cells were infected by CDV. A lot of inclusion bodies and apoptotic cells were revealed. A few of nervous cells were degeneration and shrunken with pyknotic nuclei. According to the results, it was considered that the demyelinating changes in brain tissues related mainly to the disturbance of circulation and metabolic disorder and apoptosis of ogliodendrocyles. On the whole, the causation of demyelination of brain tissues was a common active result by many factors, but not only one.

关键词： Histopathogenesis canine distemper astrocyte oligodendrocyte demyelinating encephalopathy

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Characteristic changes of T and B cells in lymphoid tissues of dogs naturally infected with canine distemper virus

Characteristic changes of T and B cells in lymphoid tissues ...

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中国畜牧兽医学会兽医病理学分第12次暨中国动物病理生理学专业委员会第11次学术讨论会

作者： takashi yoshikawa Department Veterinary Pathology School of Veterinary Medicine and Animal SciencesKitasato University35-1 Higashi-23 banchoTowada cityAomori 034-8628Japan

In order to investigate the changes of T, B cells subsets and the pathogenesis of lymphocytic depletion in lymphoid tissues this study was done in dogs naturally infected with canine distemper virus. CD3, CD45RO antigen expression of T cells and IgG, IgM antibody expression of B cells, and distribution of canine distemper virus CDV antigen were detected by immunohistochemistry. The results displayed that CDV antigen often occurred in lymphocytes, foilicular dendritic cells, lymphoid dendritic cells and macrophages of lymphoid tissues. The early myelocytes of CDV antigen positive reaction and with eosinophilic intracytoplasmic inclusion bodies were revealed in bone marrow. Compared with control group, CD3 and CD45RO - positive cells mainly presented in T cell areas. The numbers of CD3 and CD45RO - positive cells were reduced in the region of T cell. Macrophages lied in lymphoid tissue were stained by CD45RO. IgG, IgM -positive cells reduced in the zone of B cell. Some of plasma cell distributed in lymphoid tissues stained by IgG or IgM respectively. The orders of lymphocytic depletion in lymphoid tissues were, sharply IgG, moderate IgM and CD45RO, mild CD3 - positive cells. It is considered that the lymphocytic depletion in lymphoid tissues was mainly caused by B cells loss;the lower lymphocytic multiplication is significant reason to bring about lymphocytic depletion in lymphoid tissues.

关键词： canine distemper virus T cell B cell lymphoid tissue natural infection

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Zinc-Based Metal-Organic Frameworks for High-Performance Supercapacitor Electrodes:Mechanism Underlying Pore Generation

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Energy & Environmental Materials 2023年第2期6卷 100-112页

作者： Shigeyuki Umezawa takashi Douura Koji yoshikawa Daisuke Tanaka Vlad Stolojan S.Ravi P.Silva Mika Yoneda Kazuma Gotoh Yasuhiko Hayashi Seiwa Electric Mfg.Co.Ltd. Kyoto 610-0192Japan Graduate School of Natural Science and Technology Okayama UniversityOkayama 700-8350Japan School of Science and Technology Kwansei Gakuin UniversityHyogo 669-1337Japan JST PRESTO2-1 GakuenHyogo 669-1337Japan Advanced Technology Institute University of SurreyGuilfordSurreyGU27XHUK

Heat treatment of metal-organic frameworks(MOFs)has provided a wide variety of functional carbons coordinated with metal *** this study,two kinds of zinc-based MOF(ZMOF),C_(16)H_(10)O_(4)Zn(ZMOF1)and C_(8)H_(4)O_(4)Zn(ZMOF2),were ***1 and ZMOF2 were carbonized at 1000℃,forming CZMOF1 and CZMOF2,*** specific surface area(S_(BET))of CZMOF2 was~2700 m^(2)g^(−1),much higher than that of CZMOF1(~1300 m^(2)g^(−1)).A supercapacitor electrode based on CZMOF2 achieved specific capacitances of 360,278,and 221 F g^(−1)at 50,250,and 1000 mA g^(−1)in an aqueous electrolyte(H2SO_(4)),respectively,the highest values reported to date for ZMOF-derived electrodes under identical *** practical applicability of the CZMOF-based supercapacitor was verified in non-aqueous *** initial capacitance retention was 78%after 100000 charge/discharge cycles at 10 A g^(−1).Crucially,the high capacitance of CZMOF2 arises from pore generation during *** 1000℃,pore generation is dominated by the Zn/C ratio of ZMOFs,as carbon atoms reduce the zinc oxides formed during *** 1000℃,a high O/C ratio becomes essential for pore generation because the oxygen functional groups are *** findings will provide insightful information for other metal-based MOFderived multifunctional carbons.

关键词： metal-organic frameworks pore generation porous carbons supercapacitor zinc oxides

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Cyclooxygenase-2 expression after preoperative chemoradiotherapy correlates with more frequent esophageal cancer recurrence

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World Journal of Gastroenterology 2007年第16期13卷 2283-2288页

作者： Reigetsu yoshikawa Yoshinori Fujiwara Kenji Koishi Syoudou Kojima Tomohiro Matsumoto Hidenori Yanagi Takehira Yamamura Tomoko Hashimoto-Tamaoki takashi Nishigami Tohru Tsujimura Second Department of Surgery Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Institute for Advanced Medical Sciences Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Second Department of Surgery Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Institute for Advanced Medical Sciences Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Department of Genetics Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Second Department of Pathology Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan

AIM： To investigate the relationship between cycloo- xygenase-2 （COX-2）, and vascular endothelial growth factor （VEGF）, and to determine the clinical significance of this relationship in esophageal cancer patients undergoing chemoradiotherapy （CRT）. METHODS： Immunohistochemical staining was used to evaluate COX-2 and VEGF expression in 40 patients with histologically-confirmed esophageal squamous carcinoma （ESCC） who were undergoing preoperative CRT. RESULTS： Fourteen out of 40 ESCC patients showed a pathological complete response （CR） after CRT. COX-2 and VEGF protein expressions were observed in the cytoplasm of 17 and 13 tumors, respectively, with null expression in 9 and 13 tumors, respectively. COX-2 expression was strongly correlated with VEGF expression （P 〈 0.05）. There were also significant associations between COX-2 expression, tumor recurrence, and lymph-node involvement （P = 0.0277 and P = 0.0095, respectively）. COX-2 expression and VEGF expression had significant prognostic value for disease-free survival （log-rank test; P = 0.0073 and P = 0.0341, respectively）, but not for overall survival, as assessed by univariate analysis. expression correlates with VEGF expression and might be a useful prognostic factor for more frequent tumor recurrence in ESCC patients undergoing neoadjuvant CRT. These findings support the use of anti-angiogenic COX-2 inhibitors in the treatment of ESCC.

关键词： Chemoradiotherapy Cyclooxygenase-2 Esophageal cancer Metastasis Vascular endothelial growth factor

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A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone

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Bone Research 2020年第4期8卷 429-438页

作者： Junichi Kushioka takashi Kaito Rintaro Okada Hiroyuki Ishiguro Zeynep Bal Joe Kodama Ryota Chijimatsu Melanie Pye Masahiro Narimatsu Jeffrey LWrana Yasumichi Inoue Hiroko Ninomiya Shin Yamamoto takashi Saitou Hideki yoshikawa Takeshi Imamura Department of Orthopaedic Surgery Osaka University Graduate School of Medicine2-2 YamadaokaSuitaOsaka 565-0871Japan Bone and Cartilage Regenerative Medicine The University of Tokyo7-3-1HongoBunkyo-kuTokyo 113-8655Japan Centre for Systems Biology Lunenfeld-Tanenbaum Research InstituteMount Sinai Hospital600 University AveTorontoON M5G 1X5Canada Department of Cell Signaling Graduate School of Pharmaceutical SciencesNagoya City University3-1 Tanabe-doriMizuho-kuNagoyaAichi 467-8603Japan Department of Molecular Medicine for Pathogenesis Ehime University Graduate School of Medicine454 ShitsukawaToonEhime 791-0295Japan Department of Gastroenterology and Metabology Ehime University Graduate School of Medicine454 ShitsukawaToonEhime 791-0295Japan Translational Research Center Ehime University Hospital454 ShitsukawaToonEhime 791-0295Japan

Transforming growth factor-β(TGF-β)and bone morphogenetic protein(BMP)play important roles in bone *** ubiquitination regulatory factors(Smurfs)regulate TGF-β/BMP signaling via ubiquitination,resulting in degradation of signaling molecules to prevent excessive activation of TGF-β/BMP *** Smurf2 has been shown to negatively regulate TGF-β/Smad signaling,its involvement in BMP/Smad signaling in bone metabolism has not been thoroughly *** the present study,we sought to evaluate the role of Smurf2 in BMP/Smad signaling in bone *** collagen sponges containing 3μg of recombinant human BMP2(rhBMP2)were implanted in the dorsal muscle pouches of wild type(WT)and Smurf2−/−*** rhBMP2-induced ectopic bone in Smurf2−/−mice showed greater bone mass,higher mineral apposition and bone formation rates,and greater osteoblast numbers than the ectopic bone in WT *** WT mice,the ectopic bone consisted of a thin discontinuous outer cortical shell and scant inner trabecular *** contrast,in Smurf2−/−mice,the induced bone consisted of a thick,continuous outer cortical shell and abundant inner trabecular ***,rhBMP2-stimulated bone marrow stromal cells(BMSCs)from Smurf2−/−mice showed increased osteogenic ***2 induced the ubiquitination of Smad1/***/Smad signaling was enhanced in Smurf2−/−BMSCs stimulated with rhBMP2,and the inhibition of BMP/Smad signaling suppressed osteogenic differentiation of these *** findings demonstrate that Smurf2 negatively regulates BMP/Smad signaling,thereby identifying a new regulatory mechanism in bone metabolism.

关键词： Smurf2 metabolism involvement

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Comparison between colorectal low- and high-grade mucinous adenocarcinoma with MUC1 and MUC5AC

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World Journal of Gastrointestinal Oncology 2009年第1期1卷 69-73页

作者： Masayuki Onodera takashi Nishigami Ikuko Torii Ayuko Sato Li-Hua Tao Tatsuki R Kataoka Reigetsu yoshikawa Tohru Tsujimura Department of Pathology Hyogo College of Medicine Department of Surgery Hyogo College of Medicine

AIM:To explore useful prognostic factors for mucinous adenocarcinoma(MAC) in the colon and ***:MAC was divided into low-and high-grade types based on the degree of structural differentiation;low-grade MAC arisen from well to moderately differentiated adenocarcinoma and papillary carcinoma,and high-grade MAC from poorly differentiated adenocarcinoma and signet ring cell ***,the expression of 2 types of MUC1(MUC1/DF and MUC1/CORE),MUC2,2 types of MUC5AC(MUC5AC/CHL2 and HGM),MUC6,CDX2,and CD10 was examined in 16 cases of MAC consisting of 6 low-and 10 high-grade ***:MUC1/DF3 was expressed in 3 of 6 low-grade MAC(50) and 10 of 10 high-grade MAC(100).MUC1/CORE was expressed in 1 of 6 lowgrade MAC(16.7) and 7 of 10 high-grade MAC(70).MUC2 was expressed in all MAC regardless of the ***5AC was expressed in 6 of 6 low-grade MAC(100) and 4 of 10 high-grade MAC(40).HGM was expressed in 5 of 6 low-grade MAC(83.3) and 6 of 10 high-grade MAC(60).Expression of MUC6 and CD10 was undetected in all MAC regardless of the ***2 was expressed in 5 of 6 low-grade MAC(83.3) and 7 of 10 high-grade MAC(70).Taken together,MUC1/DF3 was expressed significantly more frequently in high-grade MAC than in low-grade,and MUC5AC/CHL2 was expressed significantly more frequently in low-grade MAC than in ***:It is proposed that MUC1/DF3 and MUC5AC/CHL2 immunostaining is useful to discriminate high-grade MAC from low-grade MAC.

关键词： Mucinous adenocarcinoma Colon Rectum MUC1 MUC5AC

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Colorectal neuroendocrine carcinoma: A case report and review of the literature

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World Journal of Clinical Cases 2019年第14期7卷 1865-1875页

作者： Tomoaki Yoshida Kenya Kamimura Kazunori Hosaka Koji Doumori Hiromitsu Oka Akito Sato Yasuo Fukuhara Shoji Watanabe Tomomi Sato Akira yoshikawa takashi Tomidokoro Shuji Terai Division of Gastroenterology and Hepatology Graduate School of Medical and Dental SciencesNiigata UniversityNiigata 951-8510Japan Division of Gastroenterology and Hepatology Nagaoka Chuo General HospitalNiigata 940-0861Japan

BACKGROUND Colorectal neuroendocrine carcinoma (NEC) is a rare tumor that demonstrates aggressive growth pattern with ingrowth into the tract, metastasis to the other organs, and invasion to the surrounding organs;these clinical characteristics result in poor prognosis. Surgical resection appears as an effective approach;however, because it is difficult to accurately diagnose NEC during the early stage and owing to its aggressive growth pattern, development of a reliable standard chemotherapy regimen and management strategies are essential. CASE SUMMARY Here, we report the case of patient with NEC showing an aggressive growth pattern that resulted in the rupture of the tumor to the outside the colon after stenting of the internal colonic stenosis. In addition, the tumor invaded into the duodenum, thereby causing duodenal stenosis that required an additional stent in the duodenum. This aggressive growth pattern is one of the main features of the NEC that is different from adenocarcinoma. To clarify the clinical characteristics, we reviewed 60 recently reported cases, including data on tumor location, size, treatment, and prognosis. CONCLUSION We consider that the information presented here is of great significance for the diagnosis, treatment, and management of symptoms of the patients with NEC.

关键词： Neuroendocrine carcinoma Colon Colorectal mixed adenoneuroendocrine carcinoma Growth Case report

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Association of depressive symptoms with dietary habits among Japanese physicians

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Open Journal of Preventive Medicine 2012年第3期2卷 287-290页

作者： Koji Wada Yukiko Suehiro Hiroshi Ohta Toru yoshikawa takashi Hosaka Institute for Science of Labour Kawasaki Japan Kitasato University School of Medicine Kanagawa Japan St. Luke’s International Hospital Tokyo Japan University of Occupational and Environmental Health Kitakyusyu Japan

The aim of this study was to determine the association of depressive symptoms with dietary habits among physicians working in hospitals in Japan. We mailed an anonymous questionnaire with the Quick Inventory of Depressive Symptomatology and items about dietary habits to 10,000 randomly selected physicians. Logistic regression analysis was used to explore the association of depressive symptoms with dietary habits. The participants comprised 3862 physicians (3025 men and 837 women). Among the respondents, 252 (8.3%) men and 88 (10.5%) women were determined to be in depressive symptoms. For men, “never eat a balanced diet” (Odds ratio;95% confidence interval, 2.54;1.70 - 3.80), and for women “eat quite small meals” (3.81;1.14 - 13.1), “always eat until full” (4.40;1.48 - 13.1), and “never eat a balanced diet” (3.05;1.49 - 6.23) were associated with depressive symptoms. For men, “do not eat too much” (0.57;0.39 - 0.83) was associated with prevention of depression. Physicians should recognize the important role of healthy dietary habits in good mental health. Hospitals should intervene to help physicians address depressive symptoms and dietary habits.

关键词： Physicians Depression Nutrition Food Habits

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新辅助化疗后行腹腔镜辅助胃切除术治疗晚期胃癌

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中国普通外科杂志 2013年第10期22卷 1238-1242页

作者： Takaki yoshikawa takashi Oshima Yasushi Rino Munetaka Masuda 日本横滨神奈川癌症中心胃肠外科日本横滨市立大学医学院外科

目前治疗晚期胃癌的方法为开腹胃切除及D2淋巴结清扫并在术后行S-1或卡倍他滨加奥沙利铂辅助化疗。然而III期胃癌患者的预后并不理想。为了提高生存率,需要加大化疗药物的剂量。鉴于患者接受二联或三联化疗的顺应性差,新辅助化疗将是一... 详细信息

目前治疗晚期胃癌的方法为开腹胃切除及D2淋巴结清扫并在术后行S-1或卡倍他滨加奥沙利铂辅助化疗。然而III期胃癌患者的预后并不理想。为了提高生存率,需要加大化疗药物的剂量。鉴于患者接受二联或三联化疗的顺应性差,新辅助化疗将是一个充满前景的方法。目前在东亚国家,正进行多项III期临床试验用于评估新辅助化疗效果。另一方面,手术治疗方法已倾向于行腹腔镜手术。在日本和韩国已经进行了多项III期临床试验评估腹腔镜辅助远端胃切除(LADG)在早期和晚期患者中的疗效。因此,未来标准治疗肿瘤位于中、下1/3晚期胃癌的候选方案由综合治疗组成,包括新辅助化疗及随后的LADG。只要新辅助化疗是按标准实施,随后的腹腔镜胃切除术的可行性、安全性及长期生存率是有保障的。在这个背景下,我们实施了一项随机II期临床试验比较新辅助化疗后LADG和开腹远端胃切除术(ODG)治疗胃癌的疗效。

关键词：胃肿瘤腹腔镜胃切除术新辅助化疗

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