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LC-MS/MS method for the quantitation of serum tocilizumab in rheumatoid arthritis patients using rapid tryptic digestion without IgG purification

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Journal of Pharmaceutical Analysis 2022年第6期12卷 852-859页

作者： takashi mochizuki Kaito Shibata Takafumi Naito Kumiko Shimoyama Noriyoshi Ogawa Masato Maekawa Junichi Kawakami Department of Hospital Pharmacy Hamamatsu University School of MedicineHamamatsu431-3192Japan Department of Pharmacy Shinshu University HospitalMatsumotoNagano390-8621Japan Third Division Department of Internal MedicineHamamatsu University School of MedicineHamamatsu431-3192Japan Department of Laboratory Medicine Hamamatsu University School of MedicineHamamatsu431-3192Japan

The quantitation of serum tocilizumab using liquid chromatography tandem-mass spectrometry(LC-MS/MS)method has not been widely applied in clinical settings because of its time-consuming and costly sample pretreatments.The present study aimed to develop a validated LC-MS/MS method for detecting serum tocilizumab by utilizing immobilized trypsin without an immunoglobulin G purification step and evaluate its applicability in the treatment of rheumatoid arthritis(RA)patients administered intravenously or subcutaneously with tocilizumab.The tocilizumab-derived signature peptide was deciphered using a nano-LC system coupled to a hybrid quadrupole-orbitrap mass spectrometer.The serum tocilizumab was rapidly digested by immobilized trypsin for 30 min.The chromatographic peak of the signature peptide and that of the internal standard were separated from the serum digests for a total run time of 15 min.The calibration curve of serum tocilizumab concentration was linear with a range of 2-200 μg/mL.The intra-and inter-day accuracy and relative standard deviation(RSD)were 90.7%-109.4%and<10%,respectively.The serum tocilizumab concentrations in the RA patients receiving intravenous and subcutaneous injections were 5.8-28.9 and 2.4-63.5 μg/mL,respectively.The serum tocilizumab concentrations using the current method positively correlated with those using the enzyme-linked immunosorbent assay,although a systematic error was observed between these methods.In conclusion,a validated LC-MS/MS method with minimal sample pretreatments for monitoring serum tocilizumab concentrations in RA patients was developed.

关键词： Tocilizumab LC-MS/MS Immobilized trypsin IgG purification Proteomics Rheumatoid arthritis

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游戏开发商竞逐手机存储空间

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商业周刊（中文版） 2022年第22期 25-26页

作者： takashi mochizuki 王忠(译) 不详

从东京到旧金山,手游工作室为了吸引变化无常的玩家已经争斗了多年,但却忽视了一个问题——通常游戏的体量已经非常庞大,而玩家的手机容纳不了几款游戏。日本游戏发行商Gree Inc.预计,随着开发商在游戏中加人越来越复杂的画面、音效和... 详细信息

从东京到旧金山,手游工作室为了吸引变化无常的玩家已经争斗了多年,但却忽视了一个问题——通常游戏的体量已经非常庞大,而玩家的手机容纳不了几款游戏。日本游戏发行商Gree Inc.预计,随着开发商在游戏中加人越来越复杂的画面、音效和更丰富的故事情节一一这些都是为了让玩家花钱一一他们即将面临成本上升和文件体积不断增大的问题。

关键词：存储空间文件体积手游手机游戏开发商工作室玩家旧金山

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造价1美元的芯片短缺何以在全球经济中掀起危机

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商业周刊（中文版） 2021年第6期 14-15页

作者： Debby Wu takashi mochizuki 不详

要理解4500亿美元的半导体行业何以陷入危机,造价1美元的显示驱动是个不错的切入点。彭博新闻社2021年4月6日报道称,成百上千种不同类型的芯片构成了全球半导体产业,其中最高端的高通和英特尔芯片从100美元到1000美元以上不等。计算机... 详细信息

要理解4500亿美元的半导体行业何以陷入危机,造价1美元的显示驱动是个不错的切入点。彭博新闻社2021年4月6日报道称,成百上千种不同类型的芯片构成了全球半导体产业,其中最高端的高通和英特尔芯片从100美元到1000美元以上不等。计算机或智能手机的运行都离不开这些芯片。相比之下,显示驱动则显得不那么起眼:唯一功能就是传输基本指令,让手机、显示器或导航系统屏幕亮起来。

关键词：显示驱动彭博新闻社基本指令智能手机计算机英特尔半导体行业半导体产业

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摆脱过于依赖中国市场腾讯、网易赴日拓展游戏业务

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商业周刊（中文版） 2021年第21期 15-15页

作者： takashi mochizuki Zhenping Huang 不详

腾讯斥资2.8亿元收购了一家日本创意工作室约九成股份,力求在动漫和视频游戏获得宝贵IP据知情人士,腾讯控股悄悄收购了一家日本创意工作室,该工作室名下有几款任天堂热门游戏,这是在日本这个动漫和漫画之乡延揽人才热潮的一部分。彭博... 详细信息

腾讯斥资2.8亿元收购了一家日本创意工作室约九成股份,力求在动漫和视频游戏获得宝贵IP据知情人士,腾讯控股悄悄收购了一家日本创意工作室,该工作室名下有几款任天堂热门游戏,这是在日本这个动漫和漫画之乡延揽人才热潮的一部分。彭博新闻社2021年11月12日报道称,由于交易尚未公开而要求匿名的知情人士说,这家总部位于深圳的娱乐巨头以超过50亿日元(约合2.8亿元人民币)的代价收购了Wake Up Interactive Ltd.约90%的股份。

关键词：彭博新闻社视频游戏腾讯收购工作室延揽人才动漫知情

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孙正义职业生涯充满令人费解的收购

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商业周刊（中文版） 2020年第22期 43-43页

作者： Pavel Alpeyev Takahiko Hyuga Giles Turner takashi mochizuki Erik Schatzker Sarah McBride 不详

就在投资者认为孙正义在控制软银集团的风险之际,这位日本亿万富豪挺进高杠杆衍生品领域,为他们增添了新的担忧理由。孙正义的职业生涯充满了令人费解的收购和战略转变,但现年63岁的他在2020年大部分时间都采取了对投资者友好的举措,看... 详细信息

就在投资者认为孙正义在控制软银集团的风险之际,这位日本亿万富豪挺进高杠杆衍生品领域,为他们增添了新的担忧理由。孙正义的职业生涯充满了令人费解的收购和战略转变,但现年63岁的他在2020年大部分时间都采取了对投资者友好的举措,看起来他似乎终于开始倾听维权投资者Elliott Management Corp.这类股东的声音。

关键词：孙正义亿万富豪职业生涯战略转变收购高杠杆衍生品

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Hemi-hepatectomy in pediatric patients using two-surgeon technique and a liver hanging maneuver

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World Journal of Gastroenterology 2011年第10期17卷 1354-1357页

作者： Kyoko mochizuki Susumu Eguchi Ryuichiro Hirose Taiichiro Kosaka Mitsuhisa Takatsuki takashi Kanematsu Department of Surgery Nagasaki University Graduate School of Biomedical Sciences

AIM: To evaluate the efficacy of the two-surgeon technique with the liver hanging maneuver (LHM) for hepatectomies in pediatric patients with hepatoblastoma. METHODS: Three pediatric patients with hepatoblastoma were enrolled in this study. Two underwent right hemi-hepatectomies and one underwent a left hemihepatectomy using the two-surgeon technique by means of saline-linked electric cautery (SLC) and the Cavitron Ultrasonic Surgical Aspirator (CUSA; Valleylab, Boulder, CO) and the LHM. RESULTS: The mean operative time during the parenchymal transections was 50 min and the mean blood loss was 235 g. There was no bile leakage from the cut surface after surgery. No macroscopic or microscopic-positive margins were observed in the hepatic transections. CONCLUSION: The two-surgeon technique using SLC and CUSA with the LHM is applicable to even pediatric patients with hepatoblastoma.

关键词：肝切除外科技术医生患者挂机切除术儿科

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Inflammatory pseudotumor of the liver and spleen diagnosed by percutaneous needle biopsy

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World Journal of Gastroenterology 2012年第1期18卷 90-95页

作者： Tsukasa Kawaguchi Kiyoshi mochizuki takashi Kizu Masanori Miyazaki Takayuki Yakushijin Shusaku Tsutsui Eiichi Morii Tetsuo Takehara Department of Gastroenterology and Hepatology Osaka University Hospital Department of Pathology Osaka University Hospital

An inflammatory pseudotumor (IPT) is a relatively rare lesion characterized by chronic inf iltration of inflammatory cells and areas of f ibrosis. IPTs are diff icult to diagnose because of the absence of specif ic symptoms or of characteristic hematological or radiological f indings. In this study, a case of a woman aged over 70 years was reported, who presented with a general malaise lasting more than two months. A computed tomography scan demonstrated a diffusely spread lesion of the liver with a portal vein occlusion and a splenic lesion surrounded by a soft density layer. Since the percutaneous liver biopsy showed f indings that suggested an IPT, although the radiological f indings did not exclude the possibility of a malignancy, we performed a percutaneous spleen biopsy to enable a more defi nitive diagnosis. The microscopic f indings from the spleen specimen lead us to a diagnosis of IPT involving the liver and spleen. Sub-sequent steroid pulse therapy was effective, and rapid resolution of the disease was observed.

关键词： Inflammatory pseudotumor Percutaneous liver biopsy Percutaneous spleen biopsy Steroid pulse therapy

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Quantitative Evaluation of Mixing Characteristics of Static Mixers by Visualization Experiments

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Journal of Flow Control, Measurement & Visualization 2018年第1期6卷 27-38页

作者： Noboru mochizuki Aya Kaide takashi Saeki Business Development Department ISEL Co. Ltd. Osaka Japan Graduate School of Science and Technology for Innovation Yamaguchi University Yamaguchi Japan

We propose a convenient way of evaluating the mixing performance of static mixers used for round pipe by conducting flow visualization experiments under the turbulent region and using water as the main stream. A fluorescent pigment, glycerin, two carboxymethyl cellulose solutions, and rapeseed oil were each injected upstream of the mixer. Three static mixer conditions were tested: 1) no static mixer;2) a Kenics-type static mixer;and 3) a multi-stacked elements (MSE) static mixer. The mixing trend downstream of the mixer in each condition and with each injection fluid was monitored using a laser and high-speed video camera system to obtain cross-sectional images. We propose suitable indexes based on the images obtained for quantitative evaluations of the mixing characteristics of static mixers.

关键词： Line Mixer Stacked Elements Pigment Mixing Performance Visualization Experiment

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Metabolism for cyclosporin A during liver regeneration after partial hepatectomy in rats

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World Journal of Gastroenterology 2008年第41期14卷 6355-6359页

作者： Shigeki Nagayoshi Yujo Kawashita Susumu Eguchi Yukio Kamohara Mitsuhisa Takatsuki Shungo Miyamoto Satoshi mochizuki Akihiko Soyama Hirotaka Tokai Masaaki Hidaka Yoshitsugu Tajima takashi Kanematsu Department of Transplantation and Digestive Surgery Graduate School of Biomedical Sciences Nagasaki University School of Medicine

AIM: To elucidate the metabolism and the effect of the cyclosporin A (CyA) as a representative immuno-suppressive drug used in transplantation in a partially hepatectomized rat model. METHODS: CyA was administered to rats that under- went a 70% hepatectomy. These rats were randomly assigned into three groups according to the dose of CyA administration as follows; (group 1) water, (group 2) 5 mg/kg CyA, (group 3) 10 mg/kg CyA. On post- operative days-1, 3, 7 and 14, the rats were killed to analyze the serum concentration of CyA, the liver re- generation ratio, biochemical or histological markers, and mRNA expression using reverse transcriptase-poly- merase chain reaction method to determine albumin and cytochrome p450 expression. RESULTS: The serum concentration of CyA in group 3 was significantly higher than group 2 during liver regeneration. CyA enhanced the liver regeneration in a dose dependent manner. The mRNA expression associ- ated with CyA metabolism was signifi cantly decreased on day 14, while preserving the albumin producing activity. CONCLUSION: These data indicate that the p-450 ac-tivity required to metabolize the CyA may be reduced during regeneration of the remnant liver after a hepa- tectomy, which may, therefore, be linked to diffi culty in controlling the optimal dose of CyA during early period of LDLT.

关键词：环孢菌素A 肝再生肝切除术疗效

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Dynamic reformation of gap junction and tight junction during re-modeling with cytokinesis.

Dynamic reformation of gap junction and tight junction durin...

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ThirdCongressoftheAsian...

作者： Masao Yamamoto takashi Ito Takahiko Ogawa Naohide Ohue takashi Kojima Toshihiro Mitaka Yoichi mochizuki Dept. Anat. HIROSHIMA Univ. Sch.Med.Hiroshima 734-8551 Dept. Anat. HIROSHIMA Univ. Sch.Med.Hiroshima 734-8551 Dept. Anat. HIROSHIMA Univ. Sch.Med.Hiroshima 734-8551 Dept. Anat. HIROSHIMA Univ. Sch.Med.Hiroshima 734-8551 Dept.Pathol. Cancer Res. SAPPORO Med. Sch.Sapporo 060-0061Japan Dept.Pathol. Cancer Res. SAPPORO Med. Sch.Sapporo 060-0061Japan Dept.Pathol. Cancer Res. SAPPORO Med. Sch.Sapporo 060-0061Japan

＜正＞Generally, each cell makes a impression not to have flexibility or mobility. However, the epithelial cell sheet always develops with mitosis, and the mitotic and surrounding cells have to move or slide each othe...

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