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American Journal of Analytical Chemistry 2013年第8期4卷 361-367页

作者： takashi fujimoto Daisuke Miura Takayuki Kato Osamu Kamo Yuichi Shimoikeda Tomoya Machinami Mitsuru Tashiro Department of Chemistry School of Sciences and Engineering Meisei University Hino Japan Solution & Marketing Division JEOL RESONANCE Inc. Akishima Japan

Complex formation of 1,6-anhydro-β-maltose and sodium ions was characterized using single-crystal X-ray crystallography and solution- and solid-state NMR spectroscopy. The 7-coordination structure, comprising two 1,6-anhydro-β-maltoses, a thiocyanate ion and a sodium ion, was identified in the crystal of the complex, where a sodium ion was positioned in the center of the pentagon. In the NMR study, the line broadening of 23Na signals and the decrease of the spin-lattice relaxation times (T1) of 23Na were observed in CD3OD in the presence of 1,6-anhydro-β-maltose, indicating complex formation.

关键词： 1 6-Anhydro-β-Maltose Complex Formation Single-Crystal X-Ray Crystallography Solid-State NMR Spectroscopy

Spectroscopic Studies on Impurity Transport of Core and Edge Plasmas in LHD

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Plasma Science and Technology 2006年第1期8卷 55-60页

作者： Shigeru MORITA Motoshi GOTO SadatsuguMUTO Ryuji KATAI Hisamichi YAMAZAKI Hideaki NOZATO AtsuslliI WAMAE Makoto ATAKE takashi fujimoto Atsushi SAKAUE Hiroaki NISHIMURA Ikuya SAKURAI Chiho MATSUMOTO Akihiro FURUZAWA Yuzuru TAWARA Mitsutoshi ARAMAKI Yuji OKUMURA Koichi SASAKI 龚先祖李建刚万宝年崔正英 National Institute for Fusion Science Toki 509-5292Japan Department of Fusion Science Graduate University for Advanced StudiesToki 509-5292Japan National Institute of Advanced Industrial Science and Technology Tsukuba 305-8563Japan Department of Engineering Physics Kyoto UniversityKyoto 606-8502Japan Institute of Laser Engineering Osaka UniversityOsaka 565-0871Japan Eco Topia Science Institute Nagoya UniversityNagoya 464-8603Japan Department of Electrical Engineering and Computer Science Nagoya UniversityNagoya 464-8603Japan Institute of Plasma Physics Chinese Academy of SciencesHefei 230031China Southwestern Institute of Physics Chengdu 610041China

Spectroscopic diagnostics have been extensively developed for studies of impurity and neutral particle transports at core and edge plasmas in LHD. Diagnostics of core plasmas are similar to a tokamak case, i.e., Zeff from visible bremsstrahlung, K-x-ray measurements from xray spectroscopy using Si（Li） detectors and a compact crystal spectrometer, and high-Z impurity diagnostics from VUV spectroscopy using a flat-field EUV spectrometer. A combination of impurity pellet injection and visible bremsstrahlung is an active tool for determination of the diffusion coeffici＇ent D and convective velocity V. Using this tool the spatial structures of D and V are obtained and discussed with a neoclassical effect. On the other hand, the spectroscopic method for edge diagnostics is considerably different from the tokamak case because of the existence of a thick ergodic layer in addition to the z-points necessarily included into the diagnostic chord view. In order to break this negative situation, Zeeman and polarization spectroscopy are adopted to LHD edge plasmas. As a result, 2-dimensional emission contours of HeI and Ha are successfully obtained. Laser absorption spectroscopy is tried to measure hydrogen neutrals directly. Radial profiles of edge impurities are also measured with a mirror-assembled 3 m VUV spectrometer. Recent results of and progress in LHD spectroscopy are briefly reviewed.

关键词： impurity transport plasma spectroscopy hydrogen neutral

Demonstration of three dopamine molecules bound to α-Synuclein: Implication of oligomerization at the initial stage

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Journal of Biophysical Chemistry 2012年第2期3卷 149-155页

作者： Sakurako Shimotakahara Yuuki Shiroyama takashi fujimoto Mai Akai Takaya Onoue Hiroko Seki Sayaka Kado Tomoya Machinami Yoichi Shibusawa Kenji Uéda Mitsuru Tashiro Chemical Analysis Center Chiba University Chiba Japan Department of Chemistry School of Sciences and Engineering Meisei University Tokyo Japan Department of Dementia and Higher Brain Function Tokyo Metropolitan Institute of Medical Science Tokyo Japan School of Life Science Tokyo University of Pharmacy and Life Science Tokyo Japan School of Pharmacy Tokyo University of Pharmacy and Life Science Tokyo Japan

α-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define neuropathological features and dementia with Lewy bodies. To investigate the role of dopamine (DA) in α-synuclein fibrillation, the structural propensities to form oligomers at the initial stage fibrillation were studied using size exclusion chromatography and various biophysical techniques. Interactions with DA were observed for wild-type α-synuclein and its mutants, A30P, E46K and A53T, using electrospray ionization mass spectrometry (ESI-MS). The results of ESI-MS indicate that an intact α-synuclein, which was not oxidized, had an ability to bind with three molecules of DA at the initial stage. Furthermore, upon binding to DA, α-synuclein oligomerizes to higher molecular weight species. These oligomers are structurally different from amyloid fibrils, as confirmed by thioflavin T and CD analysis.

关键词： α-Synuclein Dopamine Fibrillation Oligomerization Mass Spectrometry

Dopamine cannot promote oligomerization of unoxidized α-synuclein

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Journal of Biophysical Chemistry 2013年第3期4卷 110-114页

作者： Sakurako Shimotakahara Mika Matsui Chiseko Sakuma Teruaki Takahashi takashi fujimoto Kazuo Furihata Masaki Kojima Shohei Seino Tomoya Machinami Yoichi Shibusawa Kenji Uéda Mitsuru Tashiro Department of Chemistry School of Sciences and Engineering Meisei University Tokyo Japan Division of Agriculture and Agricultural Life Sciences The University of Tokyo Tokyo Japan School of Life Science Tokyo University of Pharmacy and Life Science Tokyo Japan School of Pharmacy Tokyo University of Pharmacy and Life Sciences Tokyo Japan

α-Synuclein is the major component of the filamentous Lewy bodies and Lewy neurites that define neuropathological features of Parkinson’s disease and dementia with Lewy bodies. To investigate the oligomerization process of α-synuclein in association with dopamine (DA), the structural propensities to form oligomers were studied using NMR and other biophysical techniques. The1H-15N HSQC spectra indicated that both N- and C-termini interacted with DA. Although interactions with DA were also observed in the presence of glutathione by ESI-MS, the significant suppression of oligomerization was observed in the size exclusion chromatography, suggesting that oxidations of α-synuclein are required for its oligomerization.

关键词： α-Synuclein Dopamine Glutathione Oligomerization

Demonstration of Three Dopamine Molecules Bound to α-Synuclein:Implication of Oligomerization at the Initial Stage

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In Vivo Development of Fetal Pig Kidneys in Mature Monkeys under Clinically Approved Immunosuppressant Drugs

Journal of Biophysical Chemistry 2012年第2期

作者： Sakurako Shimotakahara Yuuki Shiroyama takashi fujimoto Mai Akai Takaya Onoue Hiroko Seki Sayaka Kado Tomoya Machinami Yoichi Shibusawa Kenji Uéda Mitsuru Tashiro

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Engineering 2022年第3期8卷 65-73页

作者： Tsuyoshi Takamura Kenji Matsui Naoto Matsumoto Yatsumu Saito Toshinari fujimoto Susumu Tajiri Shuichiro Yamanaka Kei Matsumoto Akimitsu Kobayashi Izumi Yamamoto Hiroshi Sasaki Haruyuki Hirayama Hitomi Matsunari Kazuaki Nakano Hiroshi Nagashima Akihiko Kiyoshi Takao Kuroda Makoto Inoue Takeshi Miyawaki takashi Yokoo Eiji Kobayashi Division of Nephrology and Hypertension Department of Internal MedicineThe Jikei University School of MedicineTokyo 105-8461Japan Department of Urology The Jikei University School of MedicineTokyo 105-8461Japan Department of Plastic and Reconstructive Surgery The Jikei University School of MedicineTokyo 105-8461Japan Meiji University International Institute for Bio-Resource Research Kanagawa 214-8571Japan Sumitomo Dainippon Pharma Co. Ltd.Osaka-fu 564-0053Japan Department of Kidney Regenerative Medicine The Jikei University School of MedicineTokyo 105-8461Japan

Controlling the immune response with only clinically approved immunosuppressant drugs is difficult in renal heterotra ns plantation from pigs to nonhuman ***,to the best of our knowledge,no reports exist on the use of fetal pigs as kidney *** study aimed to compare the degree of transplant rejection between neonatal and fetal kidneys,with genetically unmodified pigs as donors and cynomolgus monkeys as *** left kidneys of the recipient monkeys were removed,followed by transplantation of neonatal as well as fetal pig kidneys,which had undergone vascular anastomosis at the same site,into the *** was performed with only US Food and Drug Administration-approved *** fetal kidneys were transplanted into the omentum and paraaortic regions of cynomolgus ***,the engraftment and development of the transplanted tissues were pathologically examined by sampling over time(twice in each experiment).An acute rejection was observed after a few weeks in neonatal renal grafts with vascular ***,fetal pig kidneys were spared from rejection despite the administration of the same immunosuppressive protocol to the monkeys and the recipient blood vessels flowing into the fetal *** immunogenicity of fetal kidneys in pig-monkey renal heterotransplantation was lower than that of neonatal kidneys.

关键词： Cynomolgus monkey Pig Kidney Fetal kidney Immunosuppression

The Cellulose Synthases Are Cargo of the TPLATE Adaptor Complex

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Molecular Plant 2018年第2期11卷 346-349页

作者： Clara Sanchez-Rodriguez Yanyun Shi Christopher Kesten Dongmei Zhang Gloria Sancho-Andres Alexander Ivakov Edwin R. Lampugnani Kamil Sklodowski Masaru fujimoto Akihiko Nakano Antony Bacic Ian S. Wallace takashi Ueda Daniel Van Damme Yihua Zhou Staffan Persson Max-Planck-lnstitute for Molecular Plant Physiology Am Muehlenberg 1 14476 Potsdam Germany Department of Biology ETH Zurich 8092 Zurich Switzerland State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China School of Biosciences University of Melbourne Parkvine 3010 VIC Australia ARC Centre of Excellence for Translational Photosynthesis Research School of Biology Australian National University GPO Box 475 Canberra ACT 2601 Australia Department of Biological Sciences Graduate School of Science The University of Tokyo Hongo Bunkyo-ku Tokyo 113-0033 Japan Live Cell Super-resolution Imaging Research Team RIKEN Center for Advances Photonics Hirosawa Wako Saitama 351-0198 Japan ARC Centre of Excellence in Plant Cell Walls School of Biosciences University of Melbourne Parkville 3010 VIC Australia Department of Biochemistry and Molecular Biology University of Nevada Reno NV 89557 USA Division of Cellular Dynamics National Institute for Basic Biology Okazaki Aichi 444-8585 Japan Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Gent Belgium Center for Plant Systems Biology VIB Technologiepark 927 9052 Gent Belgium present address: Laboratory of Plant Molecular Genetics Graduate School of Agricultural and Life Sciences The University of Tokyo 1-1-1 Yayoi Bunkyo-ku Tokyo 113-8657 Japan

Dear Editor Clathrin-mediated endocytosis （CME） is an evolutionary conserved mechanism by which plasma membrane （PM）-based cargo proteins are recognized by adaptor protein complexes and internalized. Apart from the canonical adaptor complex, AP-2, plant cells rely on the TPLATE complex （TPC） to execute CME （Gadeyne et al., 2014）. FT160100218, DP110100410）, the Ministry of Education, Culture, Sports, Science, and Technology of Japan （24114003, 15H04382, and 17K19412）, the European Research Council （ERC grant 682436）, the IRRTF-RNC （no. 501892） and a USA National Science Foundation CAREER Award.

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Recurrent colorectal cancer after endoscopic resectionwhen additional surgery was recommended

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World Journal of Gastroenterology 2016年第7期22卷 2336-2341页

作者： Yukiko Takatsu Yosuke Fukunaga Shunsuke Hamasaki Atsushi Ogura Jun Nagata Toshiya Nagasaki,takashi Akiyoshi Tsuyoshi Konishi Yoshiya fujimoto Satoshi Nagayama Masashi Ueno Department of Gastroenterological Surgery Cancer Institute HospitalJapanese Foundation for Cancer ResearchTokyo 135-8550Japan

AIM: To evaluate the type of recurrence after endoscopic resection in colorectal cancer patients and whether rescue was possible by salvage ***: Among 4972 patients who underwent surgical resection at our institution for primary or recurrent colorectal cancers from January 2005 to February 2015, we experienced eight recurrent colorectal cancers after endoscopic resection when additional surgical resection was ***: The recurrence patterns were: intramural local recurrence(five cases), regional lymph node recurrence(three cases), and associated with simultaneous distant metastasis(three cases). Among five cases with lymphatic invasion observed histologically in endoscopic resected specimens, four cases recurred with lymph node metastasis or distant metastasis. All cases were treated laparoscopically and curative surgery was achieved in six cases. Among four cases located in the rectum, three cases achieved preservation of the anus. Postoperative complications occurred in two cases(enteritis).CONCLUSION: For high-risk submucosal invasive colorectal cancers after endoscopic resection, additional surgical resection with lymphadenectomy is recommended, particularly in cases with lymphovascular invasion.

关键词： Colorectal neoplasms Colorectal surgery Laparoscopy Endoscopy Recurrence

Preoperative chemoradiation and extended pelvic lymphadenectomy for rectal cancer:Two distinct principles

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World Journal of Gastrointestinal Surgery 2010年第4期2卷 95-100页

作者： Tsuyoshi Konishi Toshiaki Watanabe Hirokazu Nagawa Masatoshi Oya Masashi Ueno Hiroya Kuroyanagi Yoshiya fujimoto takashi Akiyoshi Toshiharu Yamaguchi Tetsuichiro Muto Department of Gastroenterological Surgery Cancer Institute HospitalTokyo135-8550Japan Department of Surgical Oncology University of TokyoTokyo113-8655Japan Department of Surgery Teikyo University School of MedicineTokyo173-8605Japan

Extended pelvic lymphadenectomy(EPL) with total mesorectal excision(TME) has been reported to provide oncological benefit in lower rectal cancer in *** Western countries EPL is not widely accepted because of frequent morbidity but instead preoperative chemoradiation(CRT) followed by TME has been established as a standard treatment for decreasing local ***,several studies have focused on the comparison between these two distinct therapeutic approaches in Western countries and Japan.A study comparing Dutch trial data and Japanese data revealed that EPL and RT are almost equivalent in decreasing local recurrence in lower rectal cancer as compared with TME *** that almost 45 survival can be achieved by EPL even in the presence of metastatic lateral lymph nodes(LLNs),EPL performed by experienced surgeons definitely contributes to decrease local *** the other hand,a randomized controlled trial in Japan that compared EPL with conventional TME following preoperative RT revealed that EPL is associated with a higher frequency of sexual and urinary dysfunction without oncological benefits in the presence of preoperative *** this point,preoperative CRT followed by conventional TME without EPL would be a better therapeutic approach in patients without evident metastatic *** future treatment,it would be desirable to have a narrower indication for EPL using full advantage of recent improvement in image *** objective comparison of these two principles between Japan and the West is difficult due to differences in patient groups,further studies would lead to the next great step towards future improvement in treating lower rectal cancer.

关键词： Rectal cancer Extended lymphadenectomy Chemoradiation Pelvic lymph node Lateral lymph node

Treatment of colorectal carcinoids:A new paradigm

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World Journal of Gastrointestinal Surgery 2010年第5期2卷 153-156页

作者： Tsuyoshi Konishi Toshiaki Watanabe Hirokazu Nagawa Masatoshi Oya Masashi Ueno Hiroya Kuroyanagi Yoshiya fujimoto takashi Akiyoshi Toshiharu Yamaguchi Tetsuichiro Muto Department of Gastroenterological Surgery Cancer Institute Hospital Department of Surgical Oncology University of Tokyo Department of Surgery Teikyo University School of Medicine

It is often difficult to evaluate the grade of malignancy and choose an appropriate treatment for colorectal carcinoids in clinical settings. Although tumor size and depth of invasion are evidently not enough to stratify the risk of this rare tumor, the present guidelines or staging systems do not mention other clinicopathological variables. Recent studies, however, have shed light on the impact of lymphovascular invasion on the outcome of colorectal carcinoids. It has been revealed that the presence of lymphovascular invasion was among the strongest risk factors for metastasis along with tumor size and depth of invasion. Furthermore, tumors smaller than 1 cm, within submucosal invasion and without lymphovascular invasion, carry minimal risk for metastasis with 100% 5-year survival in the studies from Japan as well as from the USA. This would suggest that these tumors could be curatively treated by endoscopic resection or transanal local excision. On the other hand, colorectal carcinoids with either lymphovascular invasion or tumor size larger than 1 cm carry the risk for metastasis equivalent to adenocarcinomas. Therefore, it should be emphasized that histological examination of lymphovascular invasion is mandatory in the specimens obtained by endoscopic resection or transanal local excision, as this would provide useful information for determining the need for additional radical surgery with regional lymph node dissection. Although the present guidelines or TNM staging system do not mention the impact of lymphovascular invasion, this would be among the next promising targets in order to establish better guidelines and staging systems, particularly in early-stage colorectal carcinoids.

关键词： Lymphovascular invasion Neuroendocrine tumor Carcinoid Colorectal cancer