检索结果-南通市图书馆

A novel visual test for hydrogen sulfide on the tongue dorsum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Stomatology 2012年第4期2卷 314-321页

作者： takashi Kanehira Hirohisa hongo Junji Takehara Kozo Asano Kazumi Osada Hiroshi Izumi Yoshihiro Fujii Wataru Sakamoto Applied Microbiology Applied Bioscience Graduate School of Agricul-ture Hokkaido University Sapporo Japan Department of Physiology School of Dentistry Health Sciences University of Hokkaido Ishikari-Tobetsu Japan Division of Preventive Dentistry Graduate School of Dental Medicine Hokkaido University Sapporo Japan Institute of QOL Fuji Women’s University Ishikari Japan

Objective: The purpose of this study was to develop a novel colorimetric system for direct detection of hydrogen sulfide on the tongue dorsum from subjects with complaints of halitosis. Method: An assay system comprised of a cotton swab impregnated with zinc sulfate, attached chromatography paper, stabilizing solution, and coloring reagents for the methylene blue technique was developed. Using this system we investigated on the visualization of the hydrogen sulfide on the solid phase and possibility for evaluating the origin of oral malodor on the tongue dorsum. Results: The assay system made possible visualization of a linear curve ranging from 5.3 ng to 85 ng of hydrogen sulfide, with low reagent consumption and miniaturization. However, the sensitivity was different for gaseous and fluid samples. The biofilm of the tongue dorsum from subjects with complaints of halitosis showed different shades of methylene blue depending on the concentration of hydrogen sulfide. A positive correlation was found between hydrogen sulfide on the tongue dorsum and the concentration of VSC hydrogen sulfide in mouth breath as measured using an Oral Chroma semiconductor gas sensor. Conclusion: The results of this study suggest that the novel assay system can be used as a visual warning sensor for halitosis by measuring hydrogen sulfide on the tongue dorsum.

关键词： Halitosis Tongue dorsum VSC Hydrogen Sulfide Methylene Blue

MUC5AC/β-catenin expression and KRAS gene alteration in laterally spreading colorectal tumors

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第39期18卷 5551-5559页

作者： Kosaburo Nakae Hiroyuki Mitomi Tsuyoshi Saito Michiko Takahashi takashi Morimoto Yasuhiro Hidaka Naoto Sakamoto takashi Yao Sumio Watanabe Department of Gas-troenterology Juntendo University School of Medicine2-1-1 HongoBunkyo-kuTokyo 113-8421Japan Department of Human Pathology Juntendo University School of Medicine1-1-19 HongoBunkyo-kuTokyo 113-0033Japan

AIM： To clarify differences in mucin phenotype, prolif- erative activity and oncogenetic alteration among sub- types of colorectal laterally spreading tumor （LST）. METHODS： LSTs, defined as superficial elevated lesions greater than 10 mm in diameter with a low vertical axis, were macroscopically classified into two subtypes：（1） a granular type （Gr-LST） composed of superficially spread- ing aggregates of nodules forming a fiat-based lesion with a granulonodular and uneven surface; and （2） a non-granular type （NGr-LST） with a flat smooth surface and an absence of granulonodular formation. A total of 69 LSTs, comprising 36 Gr-LSTs and 33 NGr-LSTs, were immunohistochemically stained with MUC2, MUC5AC, MUC6, CD10 （markers of gastrointestinal cell lineage）, p53, 13-catenin and Ki-67 antibodies, and examined for alteration in exon 1 of v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog （KRAS） and exon 15 of v-raf murine sarcoma viral oncogene homologue B1 （BRAF） by poly- merase chain reaction followed by direct sequencing. RESULTS： Histologically, 15 Gr-LST samples were ad- enomas with low-grade dysplasia （LGD）, 12 were high- grade dysplasia （HGD） and 9 were adenocarcinomas invading the submucosa （INV）, while 12 NGr-LSTs demonstrated LGD, 14 HGD and 7 INV. In the proximal colon, MUC5AC expression was significantly higher in the Gr-type than the NGr-type. MUC6 was expressed only in NGr-LST. MUC2 or CD10 did not differ. P53 ex- pression demonstrated a significant stepwise increment in progression through LGD-HGD-INV with both types of LST. Nuclear β-catenin expression was significantly higher in the NGr-type. Ki-67 expression was signifi- cantly higher in the Gr-type in the lower one third zone of the tumor. In proximal, but not distal colon tumors, the incidence of KRAS provided mutation was signifi- cantly higher in the Gr-type harboring a specific muta- tional pattern （G12V）. BRAF mutations （V600E） were detected only in two Gr-LSTs. CONCLUSION： The two subtypes of LST,

关键词： Laterally spreading tumor Mucin core pro-tein Colon β-catenin Immunohistochemistry v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Directsequencing Adenoma-carcinoma sequence

A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2015年第4期8卷 612-621页

作者： Yuki Kondo takashi Fujita Munetaka Sugiyama Hiroo Fukuda Department of Biological Sciences Graduate School of Science The University of Tokyo 7-3-1 Hongo Bunkyo-ku tokyo 113-0033 Japan Botanical Gardens Graduate School of Science Tile University of Tokyo 3-7-1 Hakusan Bunkyo-ku Tokyo 112-0001 Japan

During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDRpro：GUS and TDRpro：YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF （tracheary element differentiation inhibitory factor） indicates that the key signaling TDIF-TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation.

关键词： Arabidopsis thaliana bikinin leaf-disk culture transcriptome tracheary element differentiation signaling

Simultaneous augmentation of muscle and bone by locomomimetism through calcium-PGC-1αsignaling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bone Research 2022年第4期10卷 770-783页

作者： Takehito Ono Ryosuke Denda Yuta Tsukahara takashi Nakamura Kazuo Okamoto Hiroshi Takayanagi Tomoki Nakashima Department of Cell Signaling Graduate School of Medical and Dental SciencesTokyo Medical and Dental University(TMDU)Yushima 1-5-45Bunkyo-kuTokyo 113-8549Japan Department of Orthopaedic Surgery The Jikei University School of Medicine3-25-8 Nishi-ShimbashiMinato-kuTokyo 105-8461Japan School of Dentistry Tokyo Medical and Dental University(TMDU)Yushima 1-5-45Bunkyo-kuTokyo 113-8549Japan Department of Biochemistry Tokyo Dental CollegeKandamisakicho 2-9-18Chiyoda-kuTokyo 101-0061Japan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyo 113-0033Japan Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyo 113-0033Japan

Impaired locomotion has been extensively studied worldwide because those afflicted with it have a potential risk of becoming *** exercise at times can be an effective remedy for frailty,but exercise therapy cannot be applied in all clinical *** is safer than exercise,but there are no drugs that reinforce both muscle and bone when administered *** medications increase the risk of adverse events;thus,there is a need for individual drugs targeting both *** this end,we established a novel sequential drug screening system and identified an aminoindazole derivative,locamidazole(LAMZ),which promotes both myogenesis and osteoblastogenesis while suppressing *** of this drug enhanced locomotor function,with muscle and bone significantly ***,LAMZ induced Mef2c and PGC-1αin a calcium signaling–dependent *** this signaling is activated upon physical exercise,LAMZ mimics physical ***,LAMZ is a promising therapeutic drug for locomotor diseases,including sarcopenia and osteoporosis.

关键词： drugs clinical diseases

Ultra-large-scale phase-field simulation study of ideal grain growth

在线全文

学校读者我要写书评

暂无评论

npj Computational Materials 2017年第1期3卷 247-252页

作者： Eisuke Miyoshi Tomohiro Takaki Munekazu Ohno Yasushi Shibuta Shinji Sakane takashi Shimokawabe Takayuki Aoki Graduate School of Science and Technology Kyoto Institute of TechnologyMatsugasakiSakyo-kuKyoto 606-8585Japan Faculty of Mechanical Engineering Kyoto Institute of TechnologyMatsugasakiSakyo-kuKyoto 606-8585Japan Division of Materials Science and Engineering Faculty of EngineeringHokkaido UniversityKita 13 Nishi 8Kita-kuSapporoHokkaido 060-8628Japan Department of Materials Engineering The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-8656Japan The Supercomputing Division Information Technology CenterThe University of Tokyo2-11-16 YayoiBunkyo-kuTokyo 113-8658Japan Global Scientific Information and Computing Center Tokyo Institute of Technology2-12-1 i7-3 O-okayamaMeguro-kuTokyo 152-8550Japan

Grain growth,a competitive growth of crystal grains accompanied by curvature-driven boundary migration,is one of the most fundamental phenomena in the context of metallurgy and other scientific ***,the true picture of grain growth is still controversial,even for the simplest(or‘ideal’)*** problem can be addressed only by large-scale numerical ***,we analyze ideal grain growth via ultra-large-scale phase-field simulations on a supercomputer for elucidating the corresponding authentic statistical *** performed simulations are more than ten times larger in time and space than the ones previously considered as the largest;this computational scale gives a strong indication of the achievement of true steady-state growth with statistically sufficient number of ***,we provide a comprehensive theoretical description of ideal grain growth behaviors correctly quantified by the present *** findings provide conclusive knowledge on ideal grain growth,establishing a platform for studying more realistic growth processes.

关键词： grain field establishing

A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bone Research 2020年第4期8卷 429-438页

作者： Junichi Kushioka takashi Kaito Rintaro Okada Hiroyuki Ishiguro Zeynep Bal Joe Kodama Ryota Chijimatsu Melanie Pye Masahiro Narimatsu Jeffrey LWrana Yasumichi Inoue Hiroko Ninomiya Shin Yamamoto takashi Saitou Hideki Yoshikawa Takeshi Imamura Department of Orthopaedic Surgery Osaka University Graduate School of Medicine2-2 YamadaokaSuitaOsaka 565-0871Japan Bone and Cartilage Regenerative Medicine The University of Tokyo7-3-1HongoBunkyo-kuTokyo 113-8655Japan Centre for Systems Biology Lunenfeld-Tanenbaum Research InstituteMount Sinai Hospital600 University AveTorontoON M5G 1X5Canada Department of Cell Signaling Graduate School of Pharmaceutical SciencesNagoya City University3-1 Tanabe-doriMizuho-kuNagoyaAichi 467-8603Japan Department of Molecular Medicine for Pathogenesis Ehime University Graduate School of Medicine454 ShitsukawaToonEhime 791-0295Japan Department of Gastroenterology and Metabology Ehime University Graduate School of Medicine454 ShitsukawaToonEhime 791-0295Japan Translational Research Center Ehime University Hospital454 ShitsukawaToonEhime 791-0295Japan

Transforming growth factor-β(TGF-β)and bone morphogenetic protein(BMP)play important roles in bone *** ubiquitination regulatory factors(Smurfs)regulate TGF-β/BMP signaling via ubiquitination,resulting in degradation of signaling molecules to prevent excessive activation of TGF-β/BMP *** Smurf2 has been shown to negatively regulate TGF-β/Smad signaling,its involvement in BMP/Smad signaling in bone metabolism has not been thoroughly *** the present study,we sought to evaluate the role of Smurf2 in BMP/Smad signaling in bone *** collagen sponges containing 3μg of recombinant human BMP2(rhBMP2)were implanted in the dorsal muscle pouches of wild type(WT)and Smurf2−/−*** rhBMP2-induced ectopic bone in Smurf2−/−mice showed greater bone mass,higher mineral apposition and bone formation rates,and greater osteoblast numbers than the ectopic bone in WT *** WT mice,the ectopic bone consisted of a thin discontinuous outer cortical shell and scant inner trabecular *** contrast,in Smurf2−/−mice,the induced bone consisted of a thick,continuous outer cortical shell and abundant inner trabecular ***,rhBMP2-stimulated bone marrow stromal cells(BMSCs)from Smurf2−/−mice showed increased osteogenic ***2 induced the ubiquitination of Smad1/***/Smad signaling was enhanced in Smurf2−/−BMSCs stimulated with rhBMP2,and the inhibition of BMP/Smad signaling suppressed osteogenic differentiation of these *** findings demonstrate that Smurf2 negatively regulates BMP/Smad signaling,thereby identifying a new regulatory mechanism in bone metabolism.

关键词： Smurf2 metabolism involvement

Efficacy of an orally active small-molecule inhibitor of RANKL in bone metastasis

在线全文

学校读者我要写书评

暂无评论

Bone Research 2019年第1期7卷 66-75页

作者： Yuta Nakai Kazuo Okamoto Asuka Terashima Shogo Ehata Jun Nishida Takeshi Imamura takashi Ono Hiroshi Takayanagi Department of Immunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyoJapan Department of Orthodontic Science Graduate School of Medical and Dental SciencesTokyo Medical and Dental UniversityYushima 1-5-45Bunkyo-kuTokyoJapan Department of Osteoimmunology Graduate School of Medicine and Faculty of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyoJapan Department of Molecular Pathology Graduate School of MedicineThe University of TokyoHongo 7-3-1Bunkyo-kuTokyoJapan Department of Molecular Medicine for Pathogenesis Ehime University Graduate School of MedicineShitsukawaToonEhimeJapan

Bone is one of the preferred sites for the metastasis of malignant tumours,such as breast cancer,lung cancer and malignant *** cells colonizing bone have the capacity to induce the expression of receptor activator of nuclear factor-κB ligand(RANKL),which promotes osteoclast differentiation and ***-induced osteoclastic bone resorption leads to a vicious cycle between tumours and bone cells that fuels osteolytic tumour growth,causing bone pain and ***,RANKL contributes to bone metastasis by acting as a chemoattractant to bone for tumour cells that express its receptor,*** inhibition of the RANKL–RANK pathway is a promising treatment for bone metastasis,and a human monoclonal anti-RANKL antibody,denosumab,has been used in the ***,orally available drugs targeting RANKL must be developed to increase the therapeutic benefits to *** we report the efficacy of the small-molecule RANKL inhibitor AS2676293 in treating bone metastasis using mouse *** administration of AS2676293 markedly inhibited bone metastasis of human breast cancer cells MDA-MB-231-5a-D-Luc2 as well as tumour-induced ***2676293 suppressed RANKLmediated tumour migration in the transwell assay and inhibited bone metastasis of the murine cell line B16F10,which is known not to trigger osteoclast *** on the results from this study,RANKL inhibition with a small-molecule compound constitutes a promising therapeutic strategy for treating bone metastasis by inhibiting both osteoclastic bone resorption and tumour migration to bone.

关键词： Bone tumours metastasis

Gastrointestinal stromal tumor of the stomach with a giant abscess penetrating the gastric lumen

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第16期13卷 2385-2387页

作者： Taro Osada Akihito Nagahara Tomohiro Kodani Akihiro Namihisa Masato Kawabe takashi Yoshizawa Toshifumi Ohkusa Sumio Watanabe Sumio Watanabe Department of Gastroenterology Juntendo University School of Medicine 2-1-1 Hongo Bunkyo-ku Tokyo 113-8421 Japan

Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the gastrointestinal tract. In large GISTs, cystic degeneration, necrosis and focal hemorrhage that occur inside the tumor can result in gastrointestinal bleeding. We describe a case of a 74-year old male with GIST of the stomach accompanied with a giant abscess that penetrated the gastric lumen. The patient experienced undiagnosed fever for two months prior to hospitalization. Gastrointestinal endoscopy, X-ray series and computed tomography of the patient’s abdomen revealed a gastric submucosal tumor in the fornix, with a fistula to the gastric lumen that was inundated with a great deal of pus. The mass was diagnosed as a GIST from biopsy specimens. The patient was treated by endoscopic drainage of the abscess and intravenous administration of antibiotics. Eventually, a partial gastrectomy was performed. He was also administered Imanitib mesylate as adjuvant therapy. He was followed up for 2 years and no metastasis or recurrence was recognized at the follow- up examinations. This is the first report of a patient with clearly diagnosed GIST with endoscopic evidence of an abscess penetrating into the gastric lumen.

关键词： Gastrointestinal stromal tumor Abscess Drainage

Regression model for stabilization energies associated with anion ordering in perovskite-type oxynitrides

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2019年第9期28卷 7-14页

作者： Masanori Kaneko Mikiya Fujii takashi Hisatomi Koichi Yamashita Kazunari Domen Department of Chemical System Engineering School of Engineering The University of Tokyo7-3-1 Hongo Bunkyo-ku Tokyo 113-8656 Japan Center for Energy & Environmental Science Interdisciplinary Cluster for Cutting Edge Research Shinshu University4-17-1 Wakasato Nagano-shi Nagano 380-8553 Japan

Certain perovskite-type oxynitrides have bandgaps suitable for renewable hydrogen production via photocatalytic and photoelectrochemical water splitting under visible *** the ordering of oxide and nitride anions in these materials is important because this ordering affects their semiconductor ***, the numerous possible orderings complicate systematic analyses based on density functional theory(DFT) calculations using defined elemental *** work shows that anion ordering in large-scale supercells within perovskite-type oxynitrides can be rapidly predicted based on machine learning, using BaNbO2N(capable of oxidizing water under irradiation up to 740 nm) as an *** learning allows the calculation of the total energy of BaNbO2N directly from randomly selected initial atomic placements without costly structural optimization, thus reducing the computational cost by more than 99.99%.Combined with the Metropolis Monte Carlo method, machine learning permits exploration of the stable anion orderings of large supercells without costly DFT *** work therefore demonstrates a means of predicting the properties of functional materials having complex compositions based on the most realistic elemental arrangements in conjunction with reasonable computational loads.

关键词： Machine learning Total energy Bandgap Local anion ordering Density functional theory