检索结果-南通市图书馆

Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

引用

BIOCELL 2023年第5期47卷 1095-1106页

作者： MIZUNA YANO KOTA HIROI TETSUYA YUASA KENJI INOUE OSAMU YAMAMOTO takao nakamura DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Faculty of Health Sciences Japan Healthcare UniversitySapporo0620053Japan

Background:Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal *** the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells(hiPS-CM),researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of *** our previous studies,we separately supplemented two polyunsaturated fatty acids(PUFAs),docosahexaenoic acid(DHA)or arachidonic acid(AA),to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation,fatty acid metabolism,and cellular *** enhancement in cellular contractility was attributed to the improvement in intercellular connection rather than a direct enhancement of the contractile ***:This study reports the successive results of the effects of DHA or AA supplementation on *** addition to the contractile force and mRNA measurements used in the previous study,we further investigated the effect of different cellular aggregations on the contractile force output by means of finite element analysis,measured glucose and fatty acids metabolites,and assessed cTNT and MLC2v expressions through immunofluorecsence ***:It showed that the sole supplementation of albumin-conjugated DHA or AA can be taken up by hiPS-CM without other uptake-enhancing factors,and the supplementations may activate the CD36_ERRγmetabolic *** or AA supplementation increased the cellular contractile ratio on collagen gels and AA supplementation stimulated hiPS-CM aggregation to form cellular *** enhancement effect on the hiPS-CM contractile force was modest since the increase in contractile force was not *** supplementation was more effective than DHA supplementation because it significantly upregulated mRNA expressions of P300 and ***,finite element analysis showed that the formati

关键词： Human iPS cells Cardiomyocytes Polyunsaturated fatty acid mRNA expression Contractility Fatty acid metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of docosahexaenoic acid or arachidonic acid supplementation on gene expression and contractile force of rat cardiomyocytes in primary culture

引用

BIOCELL 2021年第5期45卷 1213-1229页

作者： MIZUNA YANO YUTA UMEHARA TOMOKAZU KUDO takao nakamura TADASHI KOSAWADA ATSUYOSHI NISHINA MASAKI SAZUKA DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Graduate School of Medical Science Yamagata UniversityYamagata9909585Japan Department of Materials and Applied Chemistry College of Science and TechnologyNihon UniversityTokyo1018308Japan Department of Health and Nutrition Yamagata Prefectural Yonezawa University of Nutrition SciencesYonezawa9920025Japan

While fatty acids play essential roles in the physiology of the myocardium,conventional culture media contain little *** previously revealed that rat neonatal myocardium mainly contains docosahexaenoic(DHA),linoleic(LA),and arachidonic(AA)acids as polyunsaturated fatty acids(PUFAs),and these contents in cultured cardiomyocytes derived from fetal rats were markedly lower than those in the neonatal *** this study,we first assessed the effects of supplementation of DHA,LA,or AA on the fatty acid contents and the percentage change of contractile area in primarily cultured rat *** on this assessment,we then evaluated the effects of DHA or AA supplementation on mRNA expression and further directly measured the contractile force of cardiomyocytes with the *** study revealed that percentage change of contractile area was maximized under 20μM DHA or 50μM AA supplementation while LA supplementation did not affect this contraction index,and that a widespread upregulation tendency of the mRNA expression related to differentiation,maturity,fatty acid metabolism,and cell adhesion was seen in the cultured cardiomyocytes with supplementation of DHA or *** particular,upregulation of the gene expression of cellular adhesion molecules connexin43 and N-cadherin were remarkable,whereas the effects on differentiation and maturation were less ***,the increase of the percentage change of the contractile area of cardiomyocyte clusters in culture dishes with the supplementations was significant,whereas the enhancement of the contractile force was *** results suggest that supplementation of DHA or AA to the fetal cardiomyocyte culture may play effective roles in preventing the de-differentiation of the cardiomyocytes in culture and that the enhancement of the contractile performance may be mainly attributed to the improvement of intercellular connection.

关键词： Cardiomyocyte culture Polyunsaturated fatty acid mRNA expression Contractile force Intercellular connection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Lipoprotein lipase and obesity

引用

Health 2012年第12期4卷 1405-1412页

作者： Masataka Kusunoki Kazuhiko Tsutsumi Daisuke Sato takao nakamura Department of Biomedical Information Engineering Graduate School of Medical Science Yamagata University Yamagata Japan Department of Internal Medicine Medical Clinic Aichi Medical University Nagoya Japan Okinaka Memorial Institute for Medical Research Tokyo Japan

Obesity is one of the fast-growing major diseases in developed and developing countries. As has been persuasively argued, long-term imbalance between intake and expenditure of fat is a central factor in the etiology of obesity. Obesity aggravates insulin resistance and promotes cardiovascular diseases and atherosclerosis. We hypothesized that elevating lipoprOtein lipase (LPL) activity in skeletal muscle would cause an improvement of obesity. To test this hypothesis, we studied the effects of the LPL activator NO-1886 inobese animals. NO-1886 elevated LPL activity in skeletal muscle, and improved obesity as well as insulin resistance in obese rats. Furthermore, NO-1886 mitigated body weight gain induced by pioglitazone without suppressive effect on the adiponectin-increasing action of pioglitazone. LPL activators hold a lot of promise of curing several diseases shown above in clinical scene.

关键词： Lipoprotein Lipase Lipoprotein Lipase Activator Insulin Resistance Lipid Metabolism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of Fasting on Plasma Lipoprotein(a) in Cynomolgus Monkeys: Preliminary Experiments Results

引用

Open Journal of Animal Sciences 2014年第5期4卷 222-227页

作者： Masataka Kusunoki Kazuhiko Tsutsumi Daisuke Sato Tetsuro Miyata takao nakamura Department of Internal Medicine Medical Clinic Aichi Medical University Nagoya Japan Okinaka Memorial Institute for Medical Research Tokyo Japan Department of Biomedical Information Engineering Graduate School of Medical Science Yamagata University Yamagata Japan Vascular Center Sanno Medical Center Tokyo Japan

Plasma lipoprotein(a) (Lp(a)) is known to be a strong independent risk factor for ischemic heart disease. However, it is not easily modulated by drugs that are presently available. Lp(a) is not present in many experimental animals except for Old World monkeys. In this study, we examined whether cynomolgus monkeys are useful model for research of human plasma Lp(a), and observed that plasma Lp(a) in cynomolgus monkeys varied as much as humans. As a result of 4 day-fasting in cynomolgus monkeys, the plasma Lp(a) level decreased in a monkey with originally high Lp(a) level. The Lp(a) level continued to decrease even 3 days after banana feeding, but returned to the original level 3 days after monkey chow feeding. On the other hand, in a monkey with low Lp(a) level, fasting had no effect on the Lp(a) level. However, in the third monkey having originally high Lp(a) level, the Lp(a) was not affected by decreasing the amount of monkey chow feeding by 50%. In summary, we found that cynomolgus monkeys may be an useful model for studying the effects of food on plasma Lp(a) in place of humans, and that high Lp(a) level may be controllable by strict diet regulation.

关键词： Lipoprotein(a) Fasting Cynomolgus Monkey Banana

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pigmented Villonodular Synovitis of the Spine: Two Case Reports and a Literature Review

引用

Open Journal of Orthopedics 2025年第1期15卷 31-40页

作者： Kazumasa nakamura Hiroshi Takahashi Katsunori Fukutake Keiji Hasegawa Akihito Wada Department of Orthopedic Surgery School of Medicine Toho University Tokyo Japan

Introduction: Pigmented villonodular synovitis (PVNS) of the spine is a rare condition, with only a limited number of cases documented in the medical literature. In this study, we review imaging findings of two cases of PVNS arising in the spine. Case 1: A 52-year-old male presented with right thigh pain of unclear etiology. His condition subsequently deteriorated and he was referred to our hospital. MRI revealed a spinal tumor with high intensity on T1-weighted imaging (T1WI) and iso-intensity on T2WI in the right L2/3 intervertebral foramen. The tumor exhibited heterogeneous enhancement on contrast-enhanced MRI. Computed tomography (CT) myelography showed the presence of an epidural tumor within the right L2/3 intervertebral foramen, situated externally to the spinal canal and compressing the dura mater. Notable scalloping was observed in the posterior margin of the L2 vertebral body on CT images. Case 2: A 78-year-old male presented with a complaint of muscle weakness of the left upper limb. His gait gradually deteriorated, which led to suspicion of cervical myelopathy. A tumor was observed on plain MRI and exhibited low to iso-intensity on T1WI and high intensity on T2WI. The tumor was in the left C7/T1 intervertebral segment and the spinal cord was compressed from the left side. CT showed destruction of the medial aspect of the left C7 vertebral arch. Conclusion: In spinal PVNS, the signal intensity on MRI is dependent on the timing of hemorrhage within the tumor, and there are no distinctive features. However, if an extradural tumor is suspected and CT shows bone erosion, scalloping or osteolytic changes, PVNS should be considered as a differential diagnosis.

关键词： Spinal Pigmented Villonodular Synovitis Imaging Diagnosis Computed Tomography Magnetic Resonance Imaging

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Excited state calculations using variational quantum eigensolver with spin-restricted ansätze and automatically-adjusted constraints

引用

npj Computational Materials 2023年第1期9卷 2204-2212页

作者： Shigeki Gocho Hajime nakamura Shu Kanno Qi Gao takao Kobayashi Taichi Inagaki Miho Hatanaka School of Fundamental Science and Technology Faculty of Science and TechnologyKeio UniversityYokohama 223-8522Japan Quantum Computing Center Keio UniversityYokohama 223-8522Japan IBM Quantum–IBM Research Tokyo Tokyo 103-8510Japan Mitsubishi Chemical Corporation Science&Innovation CenterYokohama 227-8502Japan

The ground and excited state calculations at key geometries, such as the Frank–Condon (FC) and the conical intersection (CI)geometries, are essential for understanding photophysical properties. To compute these geometries on noisy intermediate-scalequantum devices, we proposed a strategy that combined a chemistry-inspired spin-restricted ansatz and a new excited statecalculation method called the variational quantum eigensolver under automatically-adjusted constraints (VQE/AC). Unlike theconventional excited state calculation method, called the variational quantum deflation, the VQE/AC does not require the pre-determination of constraint weights and has the potential to describe smooth potential energy surfaces. To validate this strategy,we performed the excited state calculations at the FC and CI geometries of ethylene and phenol blue at the complete active spaceself-consistent field (CASSCF) level of theory, and found that the energy errors were at most 2 kcal mol−1 even on the ibm_kawasakidevice.

关键词： properties quantum variational

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Amazake(Japanese fermented rice beverage)and its potential health benefits

引用

Food Science and Human Wellness 2025年第1期14卷 1-10页

作者： Klaus W.Lange Yukiko nakamura Faculty of Human Sciences University of RegensburgRegensburg 93040Germany Japan Society for Culture Science and TechnologyRegensburg 93040Germany Nara Institute of Science and Technology Ikoma 630-0192Japan

Amazake is a sweet and non-alcoholic beverage with a long history in *** are two types of amazake,koji amazake produced from rice koji and sakekasu amazake made from sake *** has been suggested to be a functional food with various health benefits and cosmetic *** efficacy of both koji amazake and sakekasu amazake in improving bowel movements and defecation has been well *** may be useful as a food with constipation relieving ***,beneficial effects of amazake on skin barrier function and skin water content in humans have been well established in randomized controlled *** findings of amazake effects on gut microbiota,intestinal environment and gut barrier function,mainly demonstrated in animal models,suggest a role of substances contained in amazake as *** evidence indicates the potential value of amazake in the management of obesity,metabolic syndrome and nonalcoholic fatty liver ***-term and excessive intake tests have demonstrated that the consumption of koji amazake is *** are few concerns regarding weight gain or increases in blood sugar *** functional compounds and mechanisms involved in the health effects of amazake need to be identified in future research.

关键词： Amazake Gut microbiota Bowel movement Skin barrier Functional food Health

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Applications of quantum computing for investigations of electronic transitions in phenylsulfonyl-carbazole TADF emitters

引用

npj Computational Materials 2021年第1期7卷 619-627页

作者： Qi Gao Gavin O.Jones Mario Motta Michihiko Sugawara Hiroshi CWatanabe takao Kobayashi Eriko Watanabe Yu-ya Ohnishi Hajime nakamura Naoki Yamamoto Mitsubishi Chemical Corporation Science&Innovation CenterYokohamaJapan Quantum Computing Center Keio UniversityYokohamaJapan IBM Quantum IBM Research-AlmadenSan JoseCAUSA Materials Informatics Initiative Yokkaichi Research CenterJSR CorporationYokkaichiMieJapan IBM Research-Tokyo TokyoJapan Department of Applied Physics and Physico-Informatics Keio UniversityYokohamaJapan

A quantum chemistry study of the first singlet(S_(1))and triplet(T_(1))excited states of phenylsulfonyl-carbazole compounds,proposed as useful thermally activated delayed fluorescence(TADF)emitters for organic light emitting diode(OLED)applications,was performed with the quantum Equation-Of-Motion Variational Quantum Eigensolver(qEOM-VQE)and Variational Quantum Deflation(VQD)algorithms on quantum simulators and *** quantum simulations were performed with double zeta quality basis sets on an active space comprising the highest occupied and lowest unoccupied molecular orbitals(HOMO,LUMO)of the TADF *** differences in energy separations between S_(1) and T_(1)(ΔEST)predicted by calculations on quantum simulators were found to be in excellent agreement with experimental *** of 17 and 88 mHa with respect to exact energies were found for excited states by using the qEOM-VQE and VQD algorithms,respectively,to perform simulations on quantum devices without error *** utilizing state tomography to purify the quantum states and correct energy values,the large errors found for unmitigated results could be improved to differences of,at most,4 mHa with respect to exact ***,excellent agreement could be found between values ofΔEST predicted by quantum simulations and those found in experiments.

关键词： quantum excited occupied

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Spontaneous pneumothorax in a 17-year-old male patient with multiple exostoses:A case report and review of the literature

引用

World Journal of Orthopedics 2021年第11期12卷 945-953页

作者： Koichi nakamura Kunihiro Asanuma Akira Shimamoto Shinji Kaneda Keisuke Yoshida Yumi Matsuyama Tomohito Hagi Tomoki nakamura Motoshi takao Akihiro Sudo Department of Orthopedic Surgery Mie University Graduate School of MedicineTsu 514-8507Mie PrefectureJapan Department of Thoracic and Cardiovascular Surgery Mie University Graduate School of MedicineTsu 514-8507Mie PrefectureJapan

BACKGROUND Multiple exostoses generally develop in the first decade of *** most frequently arise from the distal femur,proximal tibia,fibula,and proximal *** exostoses are rare,contributing to 1%-2% of all exostoses in hereditary multiple exostoses(HME).They are usually asymptomatic,but a few cases have resulted in severe thoracic *** caused by costal exostoses is rare,with only 13 previously reported *** report a new case of pneumothorax caused by costal *** SUMMARY A 17-year-old male with HME underwent surgery for removal of exostoses around his right *** months following the operation,he felt chest pain when he was playing the trumpet;however,he did not stop playing for a *** was referred to our hospital with a chief complaint of chest *** computed tomography(CT)scan revealed right pneumothorax and multiple exostoses in his right *** CT scan also revealed visceral pleura thickness and damaged lung tissues facing the exostosis of the seventh *** diagnosed that exostosis of the seventh rib induced *** exostosis resection was performed by video-assisted thoracoscopic surgery(VATS)2 wk after the *** patient’s postoperative course was uneventful,and there was no recurrence of pneumothorax for 2 *** Costal exostoses causing thoracic injuries should be resected regardless of *** must be considered in cases with apparently benign and relatively small exostoses or HME.

关键词： Costal exostosis Pneumothorax Video-assisted thoracoscopic surgery Hereditary multiple exostoses Case report Treatment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Middle Molecular Weight Heparinyl Amino Acid Derivatives (MHADs) Function as Indirect Radical Scavengers in Vitro

引用

Pharmacology & Pharmacy 2016年第3期7卷 117-123页

作者： Seiichi Takeda takao Toda Kazuki nakamura Research and Development Center Fuso Pharmaceutical Industries LTD Joto-ku Japan Department of Pharmacology School of Pharmacy and Pharmaceutical Sciences Mukogawa Women’s University Nishinomiya Japan

We conducted the novel synthesis of middle molecular weight heparinyl amino acid derivatives (MHADs) to reduce the adverse effect of heparin (HE) based on its anticoagulant activity. Subsequently, we investigated the radical scavenging effects of 12 kinds of MHAD on cultured human umbilical vein endothelial cells (HUV-ECs) damaged by oxygen free radicals using xanthine and xanthine oxidase in vitro. As a result, middle molecular weight heparinyl phenylalanine, middle molecular weight heparinyl leucine, and middle molecular weight heparinyl tyrosine showed significant protective effects on HUV-ECs. In conclusion, these three HE derivatives might be candidates for therapeutic agents to treat diseases attributed to peroxidation.

关键词： MHAD Heparin HUV-EC Oxygen Free Radical

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：