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Characterization of CD133^+ parenchymal cells in the liver:Histology and culture

World Journal of Gastroenterology 2009年第39期15卷 4896-4906页

作者： Seiichi Yoshikawa Yoh Zen takahiko fujii Yasunori Sato Tetsuo Ohta Yutaka Aoyagi Yasuni Nakanuma Department of Human Pathology Kanazawa University Graduate School of Medicine Division of Gastro-enterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences Division of Pathology Kanazawa University Hospital Department of Gastrointestinal Surgery Kanazawa University Graduate School of Medicine

AIM： To reveal the characteristics of CD133^＋ cells in the liver, METHODS： This study examined the histological characteristics of CD133^＋ cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133^＋ cells derived from human hepatocellular carcinoma （HCC） or cholangiocarcinoma cell lines. RESULTS： Immunostaining reveated constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133^＋/CK19^＋/HepPar-1. A small number of CD133^＋/CK19/HepPar-1^＋ cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC （HUH7, PLC5 and HepG2） and two cholangiocarcinoma cell lines （HuCCT1 and CCKS1）. Fluorescence-activated cell sorting （FACS） revealed that CD133^＋ and CD133 cells derived from HuH7 and HuCCT1 cells similarly produced CD133^＋ and CD133 cells during subculture. To examine the relationship between CD133^＋ cells and the side population （SP） phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133^＋/CD133 cells were almost identical in the SP and non-SP in HUH7. In addition, four different cellular populations （SP/CD133^＋, SP/CD133, nonP/CD133^＋, and non- SP/CD133） could similarly produce CD133^＋ and CD133- cells during subculture. CONCLUSION： This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines.

关键词： Cholangiocarcinoma Hepatocellular carcinoma Keratins Stem cells

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Patient Exposure Dose Delivered by Modern Multi-Detector Helical Computed Tomography

Patient Exposure Dose Delivered by Modern Multi-Detector Hel...

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The Second Asian and Oceanic Congress for Radiation Protection

作者： Shuji KOYAMA Keisuke fujii takahiko AOYAMA Chiyo KAWAURA Department of Radiological Technology School of Health SciencesNagoya University 1-1-20 Daiko-minamiHigashi-kuNagoya 461-8673 JAPAN Department of Radiological Technology School of Health SciencesNagoya University 1-1-20 Daiko-minamiHigashi-kuNagoya 461-8673 JAPAN Department of Radiological Technology School of Health SciencesNagoya University 1-1-20 Daiko-minamiHigashi-kuNagoya 461-8673 JAPAN Department of Radiological Technology School of Health SciencesNagoya University 1-1-20 Daiko-minamiHigashi-kuNagoya 461-8673 JAPAN

The purpose of this study was to clarify the level of organ doses delivered by modern multidetector helical computed tomography（MDCT）scanners（16 and 64 slices）and to assess the effect of tube current modulation（TCM）technique used in these ***-phantom dosimetry systems were constructed with adult and pediatric phantoms in which photodiode dosimeters were installed in various tissue and organ sites to measure the doses for these *** phantom was composed of three different tissue substitutes corresponding to soft tissue,cortical bone and lung,and 32 photodiode dosimeters were installed in the centroid of each tissue or organ assigned in the ICRP’s definition of the effective *** setting the phantoms on the bed of the CT scanners,we measured the dose at each organ site of the phantoms with and without using the TCM *** evaluation in adult thorax examinations for the 64-slice MDCT scanner indicated a maximum organ dose of 21mGy for thyroid without using the TCM technique,and that of 18mGy for lung with using the *** the pediatric thorax examinations for the MDCT scanner,the maximum organ dose was 21mGy for thyroid both with and without using the TCM *** doses in adult and pediatric abdominal CT examinations obtained by using TCM technique were slightly smaller than those without using the technique.

关键词： dosimetry MDCT organ dose effective dose tube current modulation

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