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Function, kinetic properties, crystallization, and regulation of microbial malate dehydrogenase

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第4期17卷 247-261页

作者： Toshiko takahashi-INIGUEZ Nelly ABURTO-RODRIGUEZ Ana Laura VILCHIS-GONZALEZ Maria Elena FLORES Departamento de Biologla Molecular y Biotecnologia lnstituto de Investigaciones BiomddicasUniversidad Nacional Autonoma de MexicoMexicoD.F.04510Mexico

Malate dehydrogenase （MDH） is an enzyme widely distributed among living organisms and is a key protein in the central oxidative pathway. It catalyzes the interconversion between malate and oxaloacetate using NAD＋ or NADP＊ as a cofactor. Surprisingly, this enzyme has been extensively studied in eukaryotes but there are few reports about this enzyme in prokaryotes. It is necessary to review the relevant information to gain a better understanding of the function of this enzyme. Our review of the data generated from studies in bacteria shows much diversity in their molecular properties, including weight, oligomeric states, cofactor and substrate binding affinities, as well as differences in the direction of the enzymatic reaction. Furthermore, due to the importance of its function, the transcription and activity of this enzyme are rigorously regulated. Crystal structures of MDH from different bacterial sources led to the identification of the regions involved in substrate and cofactor binding and the residues important for the dimer-dimer interface. This structural information allows one to make direct modifications to improve the enzyme catalysis by increasing its activity, cofactor binding capacity, substrate specificity, and thermostability. A comparative analysis of the phylogenetic reconstruction of MDH reveals interesting facts about its evolutionary history, dividing this superfamily of proteins into two principle clades and establishing relationships between MDHs from different cellular compartments from archaea, bacteria, and eukaryotes.

关键词： Malate dehydrogenase Carbon metabolism Tricarboxylic acid cycle

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An event mixing technique for Bose-Einstein correlations of two pions in photoproduction around 1 GeV

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Chinese Physics C 2016年第11期40卷 67-74页

作者：何庆华 H.Fujimura H.Fukasawa R.Hashimoto y.Honda T.Ishikawa T.Iwata S.Kaida J.Kasagi A.Kawano S.Kuwasaki K.Maeda S.Masumoto M.Miyabe F.Miyahara K.Mochizuki N.Muramatsu A.Nakamura K.Nawa S.Ogushi y.Okada y.Onodera K.Ozawa y.Sakamoto M.Sato H.Shimizu H.Sugai K.Suzuki y.Tajima S.takahashi y.Taniguchi y.Tsuchikawa H.yamazaki R.yamazaki H.y.yoshida Research Center for Electron Photon Science Tohoku University Institute of Fluid Physics China Academy of Engineering Physics Department of Physics Wakayama Medical University Department of Physics Yamagata University Department of Information Science Tohoku Gakuin University Department of Physics Tohoku University Department of Physics University of Tokyo Accelerator Laboratory KEK Institute of Particle and Nuclear Studies KEK Wakasa-wan Energy Research Center

We have developed an event mixing technique to observe Bose-Einstein correlations （BEC） between two identical neutral pions produced in photo-induced reactions in the non-perturbative QCD energy region. It is found that the missing-mass consistency cut and the pion-energy cut are essential for the event mixing method to effectively extract BEC observables. A Monte Carlo （MC） simulation is used to validate these constraints and confirms the efficiency of this method. Our work paves the way for similar BEC studies at lower energies where the multiplicity of emitted bosons is limited.

关键词： Bose-Einstein correlations photoproduction event mixing

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Perinuclear anti-neutrophil cytoplasmic antibodies (p-anca) in chronic ulcerative colitis: Experience in a Mexican institution

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World Journal of Gastroenterology 2006年第21期12卷 3406-3409页

作者： Jesus K yamamoto-Furusho Takeshi takahashi-Monroy Omar Vergara-Fernandez Edgardo Reyes Luis Uscanga Departments of Gastroenterology. Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico Departments of Surgery. Instituto Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico Departments of Pathology. Institute Nacional de Ciencias Medicas y Nutricion Salvador Zubiran Mexico City Mexico

AIM： To assess the prevalence and clinical value of p-ANCA in a sample of Mexican ulcerative colitis （UC） patients. METHODS： In a prospective, IRB-approved protocol, p-ANCA was determined in 80 patients with UC （mean age, 32 ± 12.9 years）. The severity and extension of disease were determined by clinical methods, searching a statistical association with p-ANCA status. RESULTS： p-ANCA were detected in 41 （51%） patients. Severity of disease was the only clinical variable statistically associated with their presence （P 〈 0.0001; OR = 9; CI 95% = 3.2-24.7）. CONCLUSION： The prevalence of p-ANCA was similar to that reported in other countries. Their presence was associated to UC severity, but offered no more information than the obtained by clinical methods.

关键词： Ulcerative colitis Inflammatory bowel disease Perinuclear anti-neutrophil cytoplasmic antibodies

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Expression of the tumor suppressor gene maspin and its significance in intraductal papillary mucinous neoplasms of the pancreas

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Hepatobiliary & Pancreatic Diseases International 2008年第1期7卷 86-90页

作者： Kyoko Kashima Nobuyuki Ohike Seishiro Mukai Masashi Sato Manabu takahashi Toshio Morohoshi Department of Pathology Showa University School of Medicine Department of Internal MedicineShowa University Toyosy Hospital Department of Pathology Showa University School of Medicine Department of Pathology Showa University School of Medicine Department of SurgeryShowa University School of Medicine Division of Pathology Ebara HospitalTokyo Japan

BACKGROUND: Maspin is a member of the serpin family of protease inhibitors and is thought to inhibit carcinoma invasion, metastasis, and angiogenesis and induce apoptosis. We examined maspin expression immunohistochemically and assessed its significance in intraductal papillary mucinous neoplasm (IPMN) of the pancreas. METHODS: We examined 39 surgically resected specimens of IPMN that included 17 adenomas (IPMAs), 5 borderline tumors (IPMBs), 4 non-invasive carcinomas (non-invasive IPMCs), and 13 invasive carcinomas (invasive IPMCs). Immunostaining was performed according to the EnVision ChemMate method. The degree of maspin expression was scored and assessed according to the percentage and staining intensity of positive cells. RESULTS: Maspin expression was minimal in normal pancreatic duct epithelium, whereas in IPMNs, maspin was expressed in neoplasms of all stages. Maspin expression increased with increasing grade from IPMAs, IPMBs, to non-invasive IPMCs but decreased significantly in invasive IPMCs. No specific association between maspin expression and mucin type was found. Analysis of maspin expression with respect to clinicopathologic factors in cases of invasive IPMC revealed a greater extent of invasion in cases of low maspin expression and significantly fewer apoptotic cells in the ***: Maspin was expressed at high levels in IPMNs at various stages from adenoma to invasive carcinoma, and our results suggest that maspin may be involved in the occurrence and progression of IPMN. In addition, our data suggest that the apoptosis-inducing action of maspin suppresses invasion and progression of IPMN.

关键词： maspin intraductal papillary mucinous neoplasm pancreas apoptosis immunohistochemistry

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Prediction of effects of punch shapes on tableting failure by using a multi-functional single-punch tablet press

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Asian Journal of Pharmaceutical Sciences 2017年第5期12卷 412-417页

作者： Takashi Osamura yoshiko Takeuchi Risako Onodera Masahiro Kitamura yoshiteru takahashi Kohei Tahara Hirofumi Takeuchi Laboratory of Pharmaceutical Engineering Gifu Pharmaceutical University1-25-4Daigaku-NishiGifu501-1196Japan Pharmaceutical Technology Department Sawai Pharmaceutical Co.Ltd12-34HiroshibachoSuita-ShiOsaka564-0052Japan

We previously determined 'Tableting properties' by using a multi-functional single-punch tablet press(GTP-1). We proposed plotting 'Compactability' on the x-axis against'Manufacturability' on the y-axis to allow visual evaluation of 'Tableting properties'. Various types of tableting failure occur in commercial drug production and are influenced by the amount of lubricant used and the shape of the punch. We used the GTP-1 to measure'Tableting properties' with different amounts of lubricant and compared the results with those of tableting on a commercial rotary tableting machine. Tablets compressed with a small amount of lubricant showed bad 'Manufacturability', leading to sticking of powder on *** also tested various punch shapes. The GTP-1 correctly predicted the actual tableting results for all punch shapes. With punches that were more likely to cause tableting failure, our system predicted the effects of lubricant quantity in the tablet formulation and the occurrence of sticking in the rotary tableting machine.

关键词： Tableting Formulation design Lubricant Punch shape Single-punch tablet press Losartan potassium

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Formulation design of granules prepared by wet granulation method using a multi-functional single-punch tablet press to avoid tableting failures

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Asian Journal of Pharmaceutical Sciences 2018年第2期13卷 113-119页

作者： Takashi Osamura yoshiko Takeuchi Risako Onodera Masahiro Kitamura yoshiteru takahashi Kohei Tahara Hirofumi Takeuchi Laboratory of Pharmaceutical Engineering Gifu Pharmaceutical University Pharmaceutical Technology Department Sawai Pharmaceutical Co.Ltd

We previously determined "Tableting properties" by using a multi-functional single-punch tablet press(GTP-1). We plotted "Compactability" on the x-axis against "Manufacturability"on the y-axis to allow visual evaluation of "Tableting properties". Here, we examined whether this evaluation method can be used in the formulation design of tablets prepared by wet granulation. We used the GTP-1 to measure "Tableting properties" with different amounts of binder, disintegrant, and lubricant, and compared the results with those of tableting on a commercial rotary tableting machine. Tableting failures(capping and binding in particular) occurred when samples that had been evaluated as having poor "Compactability" or"Manufacturability" on the GTP-1 were compressed on the rotary tableting machine. Thus,our evaluation method predicted tableting failure at the commercial scale. The method will prove useful for scaling up production.

关键词： tableting formulation design wet granulation capping binding single-punch tablet press

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Mechanical Properties and Microstructural Stability of Advanced Ferritic/Martensitic Steel under Irradiation

Mechanical Properties and Microstructural Stability of Advan...

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Ninth China-Japan Symposium on Materials for Advanced Energy Systems and Fission & Fusion Engineering Jointed with CAS-JSPS Core-university Program Seminar on Fusion Materials, System and Design Integration

作者： y.yano H.takahashi N.Akasaka S.Watanabe Oarai Research and Development Center Japan Atomic Energy Agency 4002 Narita-cho O-arai-machi Ibaraki 311-1393 Japan Center for Advanced Research of Energy Conversion Materials Hokkaido University Sapporo 060-8628 Japan Oarai Research and Development Center Japan Atomic Energy Agency 4002 Narita-cho O-arai-machi Ibaraki 311-1393 Japan Center for Advanced Research of Energy Conversion Materials Hokkaido University Sapporo 060-8628 Japan

The ferritic/martensitic(F/M)steels are expected to be prospective not only for the long life core material of fast reactors but also for the blanket materials of fusion reactor because of their superior swelling *** Nuclear Cycle Development Institute(JNC)has developed a 11Cr-0.5Mo-2W-V,Nb F/M steel(PNC-FMS)for core materials of next fast *** study is to evaluate the effect of neutron and electron irradiations on mechanical properties and structural development of PNC-FMS,especially the changes of tensile property and ductility in addition to irradiation damage structures under higher temperature irradiation.

关键词： Mechanical Properties and Microstructural Stability of Advanced Ferritic/Martensitic Steel under Irradiation

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DEFECT-FLUORITE OXIDES:Ln(Eu AND Gd)-MSSBAUER SPECTROSCOPIC STUDy COUPLED WITH NEW DEFECT-CRySTAL-CHEMISTRy MODEL

DEFECT-FLUORITE OXIDES:Ln(Eu AND Gd)-MSSBAUER SPECTROSCOPI...

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8th International Symposium on the Industrial Applications of the Mossbauer Effect

作者： A.Nakamura N.lgawa y.Okamoto y.Hinatsu M.takahashi M.Takeda Japan Atomic Energy Agency Advanced Science Research CenterTokaiNakaIbaraki 319-1195Japan Japan Atomic Energy Agency Quantum Beam Science DirectorateTokaiNakaIbaraki 319-1195Japan Hokkaido University Department of ChemistrySapporo 160-0810 HokkaidoJapan Toho University Department of ChemistryMiyamaFunahashiChiba 274-8510Japan

Defect-fluorite(DF) oxides as a generic term of various grossly oxygen-deficit oxygen-vacancy(V) type MLnO(V) solid solutions(ss) formed between parent fluorite(F) MO(M= Zr,Hf,Ce,Th,U,Np and Pu etc) and its V-ordered superstructure,lanthanide(Ln) and light-Ln C-type LnO(Ln= La-Lu,y,Sc,In etc), have been the object of enormous research efforts for over these several decades due to their technological importance in nuclear,ceramic and electrochemical etc ***,much has remained elusive till now as to their

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Estimation of water requirement per unit carbon fixed by Eucalyptus camaldulensis in semi-arid land of Western Australia

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Science China Earth Sciences 2002年第Z1期45卷 142-147页

作者： T. Kojima y. Tanaka S. Katoh K. Tahara N. takahashi K. yamada Department of Applied Chemistry Fac. of Eng. Seikei University Tokyo 180-8633 Japan Japan Science and Technology Corporation Tokyo 110-0015 Japan Deptartment of Fine Materials Engineering Shinshu University Nagano Pre. 386-8567 Japan

Afforestation in arid land is a promising method for carbon fixation, but the effective utili- zation of water is highly important and required. Thus, the evaluation of the amount of water per unit carbon fixed with the tree growth is required to minimize the amount of water supplied to the plants. In this research, a tree is regarded as a carbon fixation reactor with inflows of water and nutrients from roots, and CO_2 as the carbon source from leaves with outflow of water vapor from leaves and accumulation in the tree itself. In the process of photosynthesis and respiration nutri- tional elements are dissolved in water flow in trees. They do not flow out by these reactions, but are accumulated in trees. Thus, we have treated the behaviour of nutrients as a marker to evaluate the water/carbon ratio. Assuming that nutrient concentration is constant in sap, and the differences in the ratios of nutrient to carbon in living trees and dead (i.e. litter fall, etc.) are neglected, the ratio of the used water to fixed carbon is given as the ratio of nutrient to carbon in the tree body divided by the ratio of nutrient to water in sap. However, some nutrients are translocated and concentrated within the tree and some may be discarded through litter fall. Thus it is important to examine which nutrient element is the most suitable as the tracer. In this paper, the results of the above method applied to Eucalyptus camaldulensis in semi- arid land of Western Australia are shown. The value of water requirement per unit carbon fixation determined from potassium balance is between 421 kg-H_2O/kg-C for mature trees and 285 kg-H_2O/kg-C for young trees, while the values from calcium balance are much larger than these. The cause of the discrepancy between these values is discussed based on the measured element concentrations in sap and trees and the plant physiology. Finally, the actual average value through the life of a tree is suggested to fall between the two values.

关键词： carbon fixation, unit water requirement, arid land, Western Australia, Eucalyptus camaldulensis,

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Allograft loss from acute Page kidney secondary to trauma after kidney transplantation

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World Journal of Transplantation 2017年第1期7卷 88-93页

作者： Kazuhiro takahashi Rohini Prashar Krishna G Putchakayala William J Kane Jason E Denny Dean y Kim Lauren E Malinzak Transplant and Hepatobiliary Surgery Henry Ford Hospital Nephrology and Internal Medicine Henry Ford Hospital

We report a rare case of allograft loss from acute Page kidney secondary to trauma that occurred 12 years after kidney transplantation. A 67-year-old Caucasian male with a past surgical history of kidney transplant presented to the emergency department at a local hospital with left lower abdominal tenderness. He recalled that his cat, which weighs 15 lbs, jumped on his abdomen 7 d prior. On physical examination, a small tender mass was noticed at the incisional site of the kidney transplant. He was producing a normal amount of urine without hematuria. His serum creatinine level was slightly elevated from his baseline. Computer tomography revealed a large subscapular hematoma around the transplant kidney. The patient was observed to have renal trauma grade Ⅱ at the hospital over a period of three days, and he was finally transferred to a transplant center after his urine output significantly decreased. Doppler ultrasound demonstrated an extensive peri-allograft hypoechoic area and abnormal waveforms with absent arterial diastolic flow and a patent renal vein. Despite surgical decompression, the allograft failed to respond appropriately due to the delay in surgical intervention. This is the third reported case of allograft loss from acute Page kidney following kidney transplantation. This case reinforces that kidney care differs if the kidney is solitary or a transplant. Early recognition and aggressive treatments are mandatory, especially in a case with Doppler signs that are suggestive of compression.

关键词： Page kidney Kidney transplantation Trauma Subcapsular hematoma Doppler ultrasound

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