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LC-MS/MS Analysis of Lycorine and Galantamine in Human Serum Using Pentafluorophenyl Column

American Journal of Analytical Chemistry 2022年第9期13卷 300-313页

作者： Chizuko Sasaki Tatsuo Shinozuka Kuniko Yoshimura takaaki maruhashi Yasushi Asari Fumiko Satoh Department of Legal Medicine School of Medicine Kitasato University Sagamihara Japan Department of Pathophysiology Yokohama University of Pharmacy Yokohama Japan Department of Emergency and Critical Care Medicine School of Medicine Kitasato University Sagamihara Japan

Lycorine and galantamine are natural alkaloids found in Amaryllidaceae plants, such as narcissus. Narcissus leaves and roots are sometimes accidentally ingested because they resemble vegetables. Lycorine and galantamine are toxic and cause such effects as nausea, vomiting, and abdominal pain, when accidentally ingested. In a case of narcissus poisoning, the detection of lycorine and galantamine in biological samples is vital to determine whether they have been ingested. This study establishes a liquid chromatography-tandem mass spectrometry (LC/MS/MS) method to measure the lycorine and galantamine content of human serum, which can be used for mild to fatal poisoning cases. A serum pretreatment procedure was performed using acetonitrile and QuEChERS AOAC powder. The separation of the compounds was conducted using a pentafluorophenyl column, CAPCELL CORE PFP (2.1 mm I.D. × 100 mm, 2.7 μm). Lycorine, galantamine, and galantamine-d6 (internal standard) were identified by the transitions of m/z 288 → 147, m/z 288 → 213, and m/z 294 → 216, respectively. The calibration curves were linear in the ranges of 0.05 to 5 ng/mL and 5 to 100 ng/mL, with R2 > 0.999. The precision and accuracy were within the permissible range. The matrix effects of lycorine and galantamine were 94.3% - 98.4% and 87.8% - 91.1%, respectively. The extraction recovery rates of lycorine and galantamine were 101.9% - 112.7% and 95.6% - 107.1%, respectively. The present method detected lycorine and galantamine in the sera of three patients with mild poisoning that had accidentally ingested. This method is applicable in cases of lycorine and galantamine poisoning.

关键词： Lycorine Galantamine Narcissus Pentafluorophenyl Column LC/MS/MS

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LC-MS/MS Analysis of Lycorine and Galantamine in Human Serum Using Pentafluorophenyl Column

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American Journal of Analytical Chemistry（美国分析化学） 2022年第9期

作者： chizuko sasaki tatsuo shinozuka kuniko yoshimura takaaki maruhashi yasushi asari fumiko satoh

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