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Neuroendocrine carcinoma of the extrahepatic bile duct: A case report

World Journal of Gastroenterology 2016年第30期22卷 6960-6964页

作者： Yukio Oshiro Ryozo Gen Shinji Hashimoto Tatsuya Oda taiki sato Nobuhiro Ohkohchi Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Faculty of Medicine University of Tsukuba Department of Pathology Faculty of MedicineUniversity of Tsukuba

Neuroendocrine carcinoma(NEC) originating from the gastrointestinal hepatobiliary-pancreas is a rare, invasive, and progressive disease, for which the prognosis is extremely poor. The patient was a 72-year-old man referred with complaints of jaundice. He was diagnosed with middle extrahepatic cholangiocarcinoma(cT 4N1M0, c Stage Ⅳ). He underwent a right hepatectomy combined with extrahepatic bile duct and portal vein resection after percutaneous transhepatic portal vein embolization. Microscopic examination showed a large-cell neuroendocrine carcinoma according to the WHO criteria for the clinicopathologic classification of gastroenteropancreatic neuroendocrine tumors. Currently, the patient is receiving combination chemotherapy with cisplatin and etoposide for postoperative multiple liver metastases. Although NEC is difficult to diagnose preoperatively, it should be considered an uncommon alternative diagnosis.

关键词： Neuroendocrine carcinoma Extrahepatic bile duct Neuroendocrine tumor

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Molecular Evolution of the Cytochrome b Gene in Geographically Isolated Populations of Oryzias Latipes

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Journal of Life Sciences 2011年第4期5卷 282-286页

作者： Nobuhiko Asada Akira Hirai taiki Hamada Eri sato Akio Moriyama Tetsuro Takeuchi Department of Zoology Faculty of Science Okayama University of Science Kita Okayama 700-0005 Japan Misaki-cho Okayama 716-1122 Japan Hamada 3rd Junior High School Hamada 695-0101 Japan Faculty of Humanities Shujitsu University Higashi Okayama 703-8255 Japan

The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5＇ and 3＇ ends was 1,143 base pairs （bp） and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control （Hd-rR strain） and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 （K-I I strain） and AB480879 （HS strain）.

关键词： Cytochrome b RFLP diversity evolution Oryzias latipes.

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