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Biochemical Characterization of Soluble Acid and Alkaline Invertases from Shoots of Etiolated Pea Seedlings

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Journal of Integrative Plant Biology 2010年第6期52卷 536-548页

作者： Donggiun Kim So Yun Park Youngjae Chung Jongbum Park Sukchan lee taek-kyun lee Department of Biological Science Silla University South Sea Environment Research Department Korea Ocean Research and Development Institute Department of Life Science and Biotechnology Shin Gyeong University Department of Genetic Engineering Sungkyunkwan University

Soluble invertase was purified from pea （Pisum sativum L.） by sequential procedures entailing ammonium sulfate precipitation, DEAE-Sepharose column, Con-A- and Green 19-Sepharose affinity columns, hydroxyapatite column, ultra-filtration, and Sephacryl 300 gel filtration. The purified soluble acid （SAC） and alkaline （SALK） invertases had a pH optimum of 5.3 and 7.3, respectively. The temperature optimum of two invertases was 37 ℃. The effects of various concentrations of Tris-HCI, HgCI2, and CuSO4 on the activities of the two purified enzymes were examined. Tris-HCI and HgCI2 did not affect SAC activity, whereas 10 mM Tris-HCI and 0.05 mM HgCI2 inhibited SALK activity by about 50%. SAC and SALK were inhibited by 4.8 mM and 0.6 mM CuSO4 by 50%, respectively. The enzymes display typical hyperbolic saturation kinetics for sucrose hydrolysis. The Kms of SAC and SALK were determined to be 1.8 and 38.6 mM, respectively. The molecular masses of SAC shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting were 22 kDa and 45 kDa. The molecular mass of SALK was 30 kDa. Iso-electric points of the SAC and SALK were estimated to be about pH 7.0 and pH 5.7, respectively.

关键词： SAC Biochemical Characterization of Soluble Acid and Alkaline Invertases from Shoots of Etiolated Pea Seedlings

Phenol content,antioxidant and tyrosinase inhibitory activity of mangrove plants in Micronesia

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Asian Pacific Journal of Tropical Medicine 2014年第7期7卷 531-535页

作者： Sung-Suk Suh Jinik Hwang Mirye Park Heung-Sik Park taek-kyun lee South Sea Environment Research Department.Korea Institute of Ocean Science and Technology Korea South Pacific Ocean Research Center Korea Institute of Ocean Science and Technology

Objective:To find out and compare the in vitro antioxidant and tyrosinase inhibitory activities of two species of mangrove ***:Mangrove samples were harvested at the shoreline on the island of Weno,Chuuk State in *** phenol content,antioxidant activity(based on DPPH-free radical scavenging)and tyrosinase inhibitory activity in different tissues(leaves,barks and roots)of Rhizophora stylosa(***)and Sonneratia alba(***),collected from the island of ***:Total phenol content ranged from 4.87 to 11.96 mg per g of freeze dried *** highest antioxidant activity was observed in *** bark(85.5%).The highest tyrosinase inhibitory activity was found in *** ***,total phenol content and antioxidant activity were higher in methanol extracts than in aqueous ***:Taken together,the results of this study proved that mangroves can be excellent sources of antioxidant compounds.

关键词： Rhizophora stylosa Sonneratia alba Phebolics Antioxidant activity Tyrosinase inhibitory activity

Fatty acid methyl ester profiles and nutritive values of 20 marine microalgae in Korea

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Asian Pacific Journal of Tropical Medicine 2015年第3期8卷 191-196页

作者： Sung-Suk Suh So Jung Kim Jinik Hwang Mirye Park taek-kyun lee Eui-Joon Kil Sukchan lee South Sea Environment Research Department Korea Institute of Ocean Science and Technology Kyeongbuk Institute for Marine Bio-Industry Department of Genetic Engineering Sungkyunkwan University

Objecive:To screen the fatty acid(FA) composition of 20 marine microalgae species,including seven Diophyceae,six Bacillariophyeae four Chlorophyceae,two Haptophyceae and one Raphidophyceae ***:Microalgal cells cultured at the Korea Institute of Ocean Science & Technology were harvested during the late exponential growth phase and the FA composition ***:The FA composition of microalgae was *** example,seven different species of Dinophyceae were composed primarily of C14:0,C16:0.C18:0.C20:4n-6.C20:5n-3 and C22:*** C14:0.C16:0,C16:1.C18:0.C20:5n-3 and C22:6n-3 were abundant FAs in six species of *** addition,four Chlurophyceae,two Haptopkyeeae and one Raphidophyceae species all contained a high degree of C16:1 n-7[(9.2R-34.91)%and(34.48-35.04)%].C14:0[(13.34-25.96)%]and[(26.69-Z8.24)%],and C16:0[(5.89-29.15)%]and[(5.70-16.81)%].Several factors contribute to the nutritional value of *** the polyunsaturated FA content and n-3 to n-6 FA ratio,which could be used to assess the nutritional quality of ***:This study is the first comprehensive assessment of the FA composition and nutritional value of microalgae species in South Korea,and identifies the potential utility of FAs as species-specific biomarkers.

关键词： Microalgae PUFA Nutritive values DHA EPA

Detection of coat protein gene of nervous necrosis virus using loopmediated isothermal amplification

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Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 230-235页

作者： Jinik Hwang Sung-Suk Suh Mirye Park Myung-Joo Oh Jong-Oh Kim Sukchan lee taek-kyun lee South Sea Environment Research Department Korea Institute of ocean science & technology Korea University of Science and Technology Department of Aqualife Medicine Chonnam National University Department of Genetic Engineering Sungkyunkwan University

Objective:To establish a novel and highly specific loop-mediated isothermal amplification(LAMP) assay for the identification of nervous necrosis virus(NNV) ***:A set of synthesized primers was used to match the sequences of a specific region of the nnr gene from the National Center for Biotechnology Information database,not originating from NNV-infected fish,the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and *** addition,to determine species-specific LAMP primers,cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine ***:The optimized LAMP reaction carried out at 64 ℃ for 60 min,and above 4 U Bst DNA *** sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain *** LAMP assay primers were specific for the detection NNV infection in Epinephelus ***:The development of LAMP primers based on genetic information from a public database,not virus-infected samples,may provide a very simple and convenient method to identify viral infection in aquatic organisms.

关键词： Nervous necrosis virus Nodaviridae Polymerase chain reaction Loop-mediated isothermal Amplification

Skin whitening and anti-corrugation activities of glycoprotein fractions from liquid extracts of boiled sea cucumber

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Asian Pacific Journal of Tropical Medicine 2016年第10期9卷 980-984页

作者： So Jung Kim So Yun Park Sun-Mee Hong Eun-Hye Kwon taek-kyun lee Gyeongbuk Institute for Marine Bio-Industry South Sea Environment Research Department Korea Institute of Ocean Science and Technology

Objective: To determine skin whitening and wrinkle improvement efficacy, glycoprotein fractions were extracted from liquid extracts of boiled sea cucumber and their effects on tyrosine and elastase inhibitory activities were assayed. Methods: Fractions above and below 50 k Da(>50 kD a and 50 kDa enhanced tyrosinase and elastase inhibitory activities by 50.84% and 28.78%, respectively. Correlations of the >50 kD a concentration with tyrosinase inhibitory(R^2=0.968) and elastase inhibitory(R2=0.983) efficacy were significant. Conclusions: >50 kD a glycoprotein fraction isolated from liquid extracts of boiled sea cucumber, which can serve as a functional cosmetic ingredient for whitening and wrinkle improvement of skin.

关键词： Sea cucumber Glycoprotein Cytotoxicity Tyrosinase Elastase

Efficient detection of pathogen virus in sand dabs,Paralichthys olivaceus using loop-mediated isothermal amplification(LAMP)

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Acta Oceanologica Sinica 2016年第8期35卷 44-50页

作者： HWANG Jinik PARK So Yun SUH Sung-Suk PARK Mirye lee Sukchan lee taek-kyun South Sea Environment Research Department Korea Institute of Ocean Science and TechnologyGeoje 53201Republic of Korea Korea University of Science and Technology Daejeon 34113Republic of Korea Department of Genetic Engineering Sungkyunkwan UniversitySuwon 16419Republic of Korea

Viral hemorrhagic septicemia virus（VHSV） and marine birnavirus（MABV） are the causative pathogens for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of pathogen infection. The loop-mediated isothermal amplification（LAMP） of DNA is currently one of the most commonly used molecular diagnostic tools, as it is simple, quick, and easy to amplify target DNA under isothermal conditions. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of VHSV and MABV infection in fish was developed. Using a set of synthesized primers matching a specific region of the genome, the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficient detection of VHSV and MABV infection in the wild fish, Paralichthys olivaceus. Our results demonstrate the simplicity and convenience of this method for the detection of viral infection in aquatic organisms.

关键词： viral hemorrhagic septicaemia virus(VHSV) marine birnavirus(MABV) polymerase chain reaction loop-mediated isothermal amplification

Bleeding after endoscopic papillectomy and its risk factors:A single center experience of 196 cases

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Hepatobiliary & Pancreatic Diseases International 2024年第6期23卷 613-619页

作者： Jin Ho Choi Won Chul Kim Joo kyung Park Jong kyun lee Kyu taek lee Kwang Hyuck lee Department of Medicine Division of GastroenterologySamsung Medical CenterSungkyunkwan University School of MedicineSeoul 06351Korea

Background:Endoscopic papillectomy(EP)is an effective method to remove an ampulla of Vater(AoV)adenoma with minimal *** reviewed the clinical outcomes and prognosis of patients undergoing EP,including tumor recurrence and adverse ***:A total of 196 patients who underwent EP from January 2004 to December 2017 were *** information was collected through electronic medical records,and risk factors to predict postprocedural bleeding were analyzed using a multivariate logistic regression ***:A total of 93.9%patients(184/196)underwent complete *** the follow-up period,14.7%patients(27/184)experienced tumor recurrence,and two of seven surgically resected tumors were malignant.A total of 45.4%patients(89/196)experienced adverse events related to *** bleeding occurred in 16.3%of the patients(32/196),and they were all successfully treated with endoscopic hemostasis and conservative *** most frequent site of delayed bleeding was the distal end of the papillary orifice,and 56.3%(18/32)patients of delayed bleeding were classified as having mild severity,the others had moderate *** adenomatous polyposis(FAP)[odds ratio(OR)=3.80,95%confidence interval(CI):1.01-14.29;Pleeding was increased in patients with FAP syndrome and male patients,and post-EP bleeding occurred most commonly in the distal part of the AoV.

关键词： Ampullary neoplasm Endoscopic papillectomy Post-procedural bleeding Risk factor

Impact of adolescent complex regional pain syndrome on the psychopathology of young men ahead of military service: A retrospective cohort analysis of Korean conscription data

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Military Medical Research 2021年第3期8卷 363-372页

作者： Shin-Heon lee Myeong-Jin Ko taek-kyun Nam Jeong-Taik Kwon Yong-Sook Park Department of Neurosurgery College of MedicineChung-Ang UniversitySeoul 06973South Korea

Background: The relationship between physical and psychopathological features in complex regional pain syndrome(CRPS) has been a subject of constant interest, but no data are available in adolescents. Therefore, we aimed to identify the factors associated with psychopathology in adolescents with CRPS ahead of military ***: We retrospectively reviewed all conscription examinees who had completed a Military Personality Inventory(MPI) during a period between February 2013 and December 2016. A total of 63 persons with a history of CRPS(19-years of age for all) were enrolled. Basic demographic and pain-related data were analyzed to examine their association with MPI results. The mean FGR score as well as the 8 subdomain scores were compared between those with pain duration at <15 months(n=30) vs. ≥15 months(n=33). Binary MPI results(normal-abnormal) were also compared between the two ***: In multivariate analysis, abnormal MPI was associated with pain duration, with an odds ratio(OR) at 1.05 for every 1-month increase [95% confidence interval(CI) 1.02–1.08;P=0.002]. Subjects with pain duration at ≥15 months have lower faking good response score(P<0.001 vs. those with pain duration at <15 months), and higher abnormal MPI result rate, faking bad response, inconsistency, anxiety, depression, somatization, paranoid, personality disorder cluster A, and personality disorder cluster B scores(P<0.05). Pain duration was significantly associated with the MPI ***: Pain duration is associated with psychopathology in adolescents with CRPS. Psychopathologic features increased as the disease duration increased. A comprehensive understanding of time-dependent psychopathological factors could support the planning of multimodal approaches for managing adolescent CRPS.

关键词： Chronic pain Complex regional pain syndromes Psychopathology Personality inventory Adolescent Military personnel

Is cholecystectomy a reasonable treatment option for simple gallbladder polyps larger than 10 mm?

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World Journal of Gastroenterology 2015年第14期21卷 4248-4254页

作者： Hye Yon Park Se Hoon Oh Kwang Hyuck lee Jong kyun lee Kyu taek lee Division of Gastroenterology Department of Internal MedicineSamsung medical centerSungkyunkwan University School of Medicine

AIM: To determine the relevance of the 10-mm size criterion of the generally accepted surgical indication for gallbladder polyps(GBPs).METHODS: We collected data of patients who were confirmed to have GBPs through cholecystectomy at Samsung Medical Center between January 1997 andDecember *** the patients who underwent cholecystectomy for GBP, those with a definite evidence for malignancy such as adjacent organ invasion, metastasis on preoperative imaging studies, polyp larger than 20 mm, absence of preoperative imaging study results, and patients having gallstones were *** retrospectively collected and analyzed information on patient's clinical characteristics, symptoms, ultrasonographic findings, and blood laboratory ***: A total of 836 patients who had undergone cholecystectomy were retrospectively *** hundred eighty patients(93%) had benign polyps, whereas 56 patients(7%) had malignant *** the 56 patients with malignancy, 4 patients(7%) had borderline GBP(10-12 mm) and a patient had small GBP(< 10 mm) with T2 *** conducted an ROC curve analysis to verify the 10-mm size criteria(AUC = 0.887, SD = 0.21, P < 0.001).In the ROC curve for polyp size and malignancy, sensitivity and specificity of the 10-mm size criterion was 98.2% and 19.6%, *** specificity of the 11-mm and 12-mm size criteria was 44.6% and 56%, respectively, whereas the sensitivity of these two size criteria was *** defined the GBPs of 10 to 12 mm as a borderline-sized GBP, which were found in 411 patients(49%).In this group, there was a significant difference in age between patients with benign and malignant GBPs(47 years vs 60 years, P < 0.05).CONCLUSION: GBPs larger than 13 mm need immediate excision whereas for borderline-sized GBPs detected in young patients, careful medical observation can be a rational decision.

关键词： Gallbladder polyp Gallbladder cancer Cholecystecto