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Development of real-time onion disease monitoring system using image acquisition

Frontiers of Agricultural Science and Engineering 2018年第4期5卷 469-474页

作者： Du-Han KIM Kyeong-Hwan LEE Chang-Hyun choi tae-Hyun choi Yong-Joo KIM Department of Biosystems Machinery Engineering Chung-Nam National UniversityDaejeon 305-764Republic of Korea Department of Rural and Biosystems Engineering Chonnam National UniversityGwangju 500-757Republic of Korea Department of Bio-Mechatronic Engineering Sungkyunkwan UniversitySuwonGyeonggi 440-746Republic of Korea Sensoreye R&D Solutions Daejeon 302-861Republic of Korea

In this study, real-time disease monitoring was conducted on onion which is the most representative crop in Republic of Korea, using an image acquisition system newly developed for the mobile measurement of phenotype. The purpose of this study was to improve the accuracy of prediction of disease and state variables by processing images acquired from monitoring. The image acquisition system was consisted of two parts, a motorized driving system and a PTZ(pan, tilt and zoom) camera to take images of the plants. The acquired images were processed as follows. Noise was removed through an image filter and RGB(red, green and blue) colors were converted to HSV(hue, saturation and value), which enabled thresholding of areas with different colors and properties for image binarization by comparing the color of onion leaf with ambient areas. Four objects with the most significant browning in the onion leaf to the naked eye were selected as the samples for data acquired. The thresholding method with image processing was found to be superior to the naked eye in identifying accurate disease areas. In addition, it was found that the incidence of disease was different in each disease area ratio. As a result, the use of image acquisition system in image processing analysis will enable more prompt detection of any changes in the onion and monitoring of disease outbreaks during the crop lifecycle.

关键词： imaging acquisition system disease downy mildew onion

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Clinical advantages of single port laparoscopic hepatectomy

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World Journal of Gastroenterology 2018年第3期24卷 379-386页

作者： Jae Hyun Han Young Kyoung You Ho Joong choi tae Ho Hong Dong Goo Kim Division of Hepatobiliary-Pancreas Surgery and Liver Transplantation Department of SurgerySeoul St.Mary’s HospitalCollege of MedicineThe Catholic University of Korea

AIM To evaluate the clinical advantages of single-port laparoscopic hepatectomy(SPLH) compare to multiport laparoscopic hepatectomy(MPLH).METHODS We retrospectively reviewed the medical records of 246 patients who underwent laparoscopic liver resection between January 2008 and December 2015 at our hospital. We divided the surgical technique into two groups; SPLH and MPLH. We performed laparoscopic liver resection for both benign and malignant disease. Major hepatectomy such as right and left hepatectomy was also done with sufficient disease-free margin. The operative time, the volume of blood loss, transfusion rate, and the conversion rate to MPLH or open surgery was evaluated. The post-operative parameters included the meal start date after operation, the number of postoperative days spent in the hospital, and surgical complications was also *** Of the 246 patients, 155 patients underwent SPLH and 91 patients underwent MPLH. Conversion rate was 22.6% in SPLH and 19.8% in MPLH(P = 0.358). We performed major hepatectomy, which was defined as resection of more than 2 sections, in 13.5% of patients in the SPLH group and in 13.3% of patients in the MPLH group(P = 0.962). Mean operative timewas 136.9 ± 89.2 min in the SPLH group and 231.2 ± 149.7 min in the MPLH group(P < 0.001). The amount of blood loss was 385.1 ± 409.3 m L in the SPLH group and 559.9 ± 624.9 m L in the MPLH group(P = 0.016). The safety resection margin did not show a significant difference(0.84 ± 0.84 cm in SPLH vs 1.04 ± 1.22 cm in MPLH, P = 0.704). Enteral feeding was started earlier in the SPLH group(1.06 ± 0.27 d after operation) than in the MPLH group(1.63 ± 1.27 d)(P < 0.001). The mean hospital stay after operation was non-significantly shorter in the SPLH group than in the MPLH group(7.82 ± 2.79 d vs 7.97 ± 3.69 d, P = 0.744). The complication rate was not significantly different(P = 0.397) and there was no major perioperative complication or mortality case in both groups. CONCLUS

关键词： Hepatectomy Laparoscopy Minimally invasive surgery Treatment outcome Feasibility study

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Preloaded and non-preloaded intraocular lens delivery system and characteristics: human and porcine eyes trial

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International Journal of Ophthalmology(English edition) 2018年第1期11卷 6-11页

作者： Byunghoon Chung Hun Lee Moonjung choi Kyoung Yul Seo Eung Kweon Kim tae-im Kim The Institute of Vision Research Department of Ophthalmology Yonsei University College of Medicine Department of Ophthalmology International St. Mary's Hospital Catholic Kwandong University College of Medicine Corneal Dystrophy Research Institute Yonsei University College of Medicine

AIM： To compare characteristics of preloaded and nonpreloaded intraocular lens（IOL） delivery systems during IOL delivery procedures. METHODS： Total 101 human eyes were included in this prospective observational case series. Delivery characteristics of 5 types of IOLs including i Sert250 NC60（NC60）, En Vista MX60（MX60）, Acry Sof IQ SN60 WF（SN60 WF）, TECNIS ZCB00（ZCB00）, and TECNIS PCB00（PCB00） were investigated. NC60 and PCB00 were injected via preloaded delivery systems and other IOLs were injected via nonpreloaded systems. In the human trial, time taken from IOL loading to completion of implantation was measured in all eyes undergoing conventional cataract surgery. Using 4 excised porcine eyes, dynamics of ophthalmic viscosurgical device（OVD） between an IOL injector and a porcine eye was analyzed using fluorescein sodiumstained OVD. RESULTS： The average time for IOL implantation was 22.0 s for NC60, 43.2 s for MX60, 32.3 s for SN60 WF, 41.4 s for ZCB00, and 14.6 s for PCB00 respectively. The number of cases with IOL manipulation with a second instrument was 6 for MX60, 2 for ZCB00, 0 for SN60 WF, NC60, and PCB00. Amount of OVD pushed into a porcine eye was smaller with a preloaded system than with non-preloaded systems. CONCLUSION： IOL delivery with preloaded systems is faster and more predictable. Moreover, a preloaded delivery system shows relatively less OVD pushed into a porcine eye than non-preloaded systems.

关键词： preloaded intraocular lens delivery system non-preloaded intraocular lens delivery system intraocular lens delivery time ophthalmic viscosurgical device

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Does intraoperative frozen section really predict significant positive surgical margins after robot-assisted laparoscopic prostatectomy?A retrospective study

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Asian Journal of Andrology 2021年第1期23卷 74-79页

作者： Se Young choi Byung Hoon Chi tae-Hyoung Kim Bumjin Lim Wonchul Lee Dalsan You Choung-Soo Kim Department of Urology Chung-Ang University HospitalChung-Ang University College of MedicineDongjak-guSeoul 06973Korea Department of Urology Asan Medical CenterUniversity of Ulsan College of MedicineSongpa-guSeoul 05505Korea

We investigated the relationship between positive surgical margin(PSM)-related factors and biochemical recurrence(BCR)and the ability of intraoperative frozen sections to predict significant PSM in patients with prostate *** study included 271 patients who underwent robot-assisted laparoscopic prostatectomy with bilateral nerve sparing and maximal urethral *** frozen sections of the periurethra,dorsal vein,and bladder neck were *** ability of PSM-related factors to predict BCR and significant PSM was assessed by logistic *** 271 patients,108(39.9%)had PSM and 163(60.1%)had negative *** Gleason score^8(18.9%vs 7.5%,P=0.015)and T stage≥T3a(51.9%vs 24.6%,P<0.001)were significantly more frequent in the PSM *** analysis showed that Gleason pattern≥4(vs<4;hazard ratio:4.386;P=0.0004)was the only significant predictor of BCR in the PSM *** frozen sections had a sensitivity of 83.3%and a specificity of 84.2%in detecting PSM with Gleason pattern≥*** analysis showed that membranous urethra length(odds ratio[OR]:0.79,P=0.0376)and extracapsular extension of the apex(OR:4.58,P=0.0226)on magnetic resonance imaging(MRI)and positive periurethral tissue(OR:17.85,P<0.0001)were associated with PSM of the *** with Gleason pattern≥4 is significantly predictive of *** frozen sections of periurethral tissue can independently predict PSM,whereas sections of the bladder neck and dorsal vein could *** examination of these samples may help predict significant PSM in patients undergoing robot-assisted laparoscopic prostatectomy with preservation of functional outcomes.

关键词： biochemical recurrence frozen section positive surgical margin robotic-assisted prostatectomy

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Protective effects of edible insect protein extracts from Protaetia brevitarsis against H_(2)O_(2)-induced oxidative stress in mouse C2C12 myoblast cells

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Food Bioscience 2023年第2期52卷 435-443页

作者： Jae Hoon Lee tae-Kyung Kim Min-Cheol Kang Bum-Keun Kim Yun-Sang choi Research Group of Food Processing Korea Food Research InstituteWanju55365Republic of Korea

Edible insects have been attracting significant attention not only for their nutritional superiority but also for their excellent bio-functional *** this study,the apoptotic protective effects of water-soluble protein(WPB)and salt-soluble protein(SPB)extracts derived from Protaetia brevitarsis against hydrogen peroxide(H_(2)O_(2))-induced oxidative stress in C2C12 cells were *** and SPB were confirmed to exhibit radical scavenging and ferric-ion reducing *** addition,WPB and SPB treatments effectively restored the decreased cell viability of the C2C12 cells induced by H2O2 treatment,and showcased protective effects against apoptosis and mitochondrial dysfunction by regulating the expressions of pro-and anti-apoptotic ***,our data indicated that protein extracts from Protaetia brevitarsis could be beneficial for the prevention and treatment of diseases associated with apoptosis induced by oxidative stress.

关键词： Edible insect protein extract Protaetia brevitarsis Antioxidant Anti-Apoptosis C2C12 myoblast cells

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Assessment of risk of complications in cirrhosis using portal thallium scans

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World Journal of Gastroenterology 2014年第1期20卷 228-234页

作者： Hye-Jin tae Dae-Won Jun Yun-Young choi Min-Jung Kwak Min-Ho Lee Department of Internal Medicine Hanyang University School of MedicineSeoul 133-792South Korea Department of Nuclear Medicine Hanyang University College of MedicineSeoul 133-792South Korea Department of Computer Science and Statistics Pyongtaek UniversityPyongtaek 450-701South Korea

AIM:To investigate the usefulness of a novel thallium scan shunt index for assessing portosystemic shuntrelated cirrhotic ***:We enrolled 209 chronic hepatitis B-related cirrhosis *** rectal thallium instillation,radioactive isotope activity in the heart and liver was *** ratio of radiation uptake between the heart and the liver was calculated(the shunt index).This value indicates the degree of portosystemic circulation *** tests,serum biochemistry tests,abdominal ultrasonography,gastroscopy and examination of clinical features such as the occurrence of varices,bleeding and hepatic encephalopathy were *** analysis was used to identify independent risk factors for *** compared the cumulative incidence rates of complications during the follow-up ***:The thallium scan shunt index was significantly higher in the decompensated liver cirrhosis group than in the compensated liver cirrhosis group(0.91±0.39 vs 0.39±0.32,P<0.001).It was also higher in the varices group,the hepatic encephalopathy group,and the variceal bleeding group than in the control group(P<0.001).Multivariate analysis showed that the index was an independent risk factor for predicting decompensated liver *** the cut-off value was 0.75,the shunt index had a sensitivity of 82.6%,a specificity of 84%,a positive predictive value of 61.5%,and a negative predictive value of 94.4%in diagnosing decompensated *** the shunt index was greater than 0.75,there was a significant increase in the number of decompensated ***:The thallium shunt index is a good predictor of cirrhosis-related complications.

关键词： Liver cirrhosis Portosystemic shunt Decompensation

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Acupuncture for Treating Attention Deficit Hyperactivity Disorder:A Systematic Review and Meta-Analysis

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Chinese Journal of Integrative Medicine 2011年第4期17卷 257-260页

作者： Myeong Soo Lee tae-Young choi Jong-In Kim Lakhyung Kim Edzard Ernst Brain Disease Research Centre Korea Institute of Oriental MedicineDaejeon(305-811)South Korea Complementary Medicine Peninsula College of Medicine and DentistryUniversity of ExeterExeter(EX2 4NT)UK College of Oriental Medicine Kyung Hee University Medical CenterSeoul(130-702)South Korea College of Oriental Medicine Woosuk UniversityWanju(565-701)South Korea

Objective：To assess the effectiveness of acupuncture as a treatment option for attention deficit hyperactivity disorder（ADHD）.Methods：The literatures were searched using 15 databases,including MEDLINE, AMED,CINAHL,EMBASE,Psyclnfo,the Cochrane Central Register of Controlled Trials,the Cochrane Database of Systematic Reviews,six Korean medical databases and two Chinese databases without language *** controlled clinical studies of any type of acupuncture therapy for ADHD autistic patients were *** in which acupuncture was part of a complex intervention were also *** articles were read by two independent reviewers,who extracted data from the articles according to predefined criteria. Risk of bias was assessed using the Cochrane risk of bias ***：Of 114 articles,only three randomized clinical trials（RCTs） met our inclusion *** RCT found that electroacupuncture（EA） plus behavioural treatment was superior to sham EA plus behavioural *** RCTs reported a significant benefit of acupuncture or auricular acupuncture over conventional drug ***：Limited evidence exists for the effectiveness of acupuncture as a symptomatic treatment of *** that the risk of bias of the included studies was high,firm conclusions cannot be drawn.

关键词： acupuncture attention deficit hyperactivity disorder systematic review

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Single-cell RNA sequencing of mitoticarrested prospermatogonia with DAZL::GFP chickens and revealing unique epigenetic reprogramming of chickens

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1305-1325页

作者： Hyeon Jeong choi Kyung Min Jung Deivendran Rengaraj Kyung Youn Lee Eunhui Yoo tae Hyun Kim Jae Yong Han Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences Seoul National UniversitySeoul 08826South Korea Department of Animal Science Pennsylvania State UniversityState CollegePA 16801USA

Background:Germ cell mitotic arrest is conserved in many vertebrates,including birds,although the time of entry or exit into quiescence phase *** arrest is essential for the normal differentiation of male germ cells into spermatogonia and accompanies epigenetic reprogramming and meiosis inhibition from embryonic development to ***,mitotic arrest was not well studied in chickens because of the difficulty in obtaining pure germ cells from relevant developmental ***:We performed single-cell RNA sequencing to investigate transcriptional dynamics of male germ cells during mitotic arrest in DAZL::GFP *** differentially expressed gene analysis and K-means clustering to analyze cells at different developmental stages(E12,E16,and hatch),we found that metabolic and signaling pathways were regulated,and that the epigenome was reprogrammed during mitotic *** particular,we found that histone H3K9 and H3K14 acetylation(by HDAC2)and DNA demethylation(by DNMT3B and HELLS)led to a transcriptionally permissive chromatin ***,we found that global DNA demethylation occurred gradually after the onset of mitotic arrest,indicating that the epigenetic-reprogramming schedule of the chicken genome differs from that of the mammalian *** hypomethylation persisted after hatching,and methylation was slowly re-established 3 weeks ***:We found a unique epigenetic-reprogramming schedule of mitotic-arrested chicken prospermatogonia and prolonged hypomethylation after *** will provide a foundation for understanding the process of germ-cell epigenetic regulation in several species for which this process is not clearly *** findings on the biological processes related to sex-specific differentiation of prospermatogonia could help studying germline development in vitro more elaborately.

关键词： DAZL::GFP chickens DNA methylation Epigenetic reprogramming Histone acetylation Male germ cell Mitotic arrest Prospermatogonia Single-cell RNA sequencing

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Trilobite Biostratigraphy of the lower Paleozoic(Cambrian–Ordovician)Joseon Supergroup,taebaeksan Basin,Korea

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Acta Geologica Sinica(English Edition) 2016年第6期90卷 1976-1999页

作者： choi Duck K LEE Jeong Gu LEE Seung-Bae PARK,tae-Yoon S. HONG Paul S. Research Institute of Basic Sciences Seoul National UniversitySeoul 151-747Korea Ministry of Science ICTand Future PlanningGwacheon 427-700Korea Korea Institute of Geoscience and Mineral Resources Daejeon 305-350Korea Korea Polar Research Institute Incheon 406-840Korea

In Korea, trilobites are among the most intensively studied fossil groups in the past century and provide invaluable information about lower Paleozoic stratigraphy, paleogeography, and tectonics of the Korean Peninsula. Trilobites occur in the lower Paleozoic Joseon Supergroup of the taebaeksan Basin which was part of the Sino-Korean Craton in the Paleozoic. The Joseon Supergroup is divided into the taebaek, Yeongwol, and Mungyeong groups. The taebaek and Yeongwol groups are richly fossiliferous, while the Mungyeong Group is poorly fossiliferous. Contrasting trilobite faunal contents of the taebaek and Yeongwol groups resulted in two separate biostratigraphic schemes for the Cambrian–Ordovician of the taebaeksan Basin. A total of 22 biozones or fossiliferous horizons were recognized in the taebaek Group; 19 zones were established in the Yeongwol Group; and four biozones were known from the Mungyeong Group. These trilobite biozones of the taebaeksan Basin indicate the Joseon Supergroup ranges in age from the Cambrian Series 2 to Middle Ordovician and can be correlated well with the formations of North China, South China, and Australia.

关键词： Trilobites biostratigraphy Cambrian–Ordovician taebaeksan Basin

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Characterization of MAGEG2 with testis-specific expression in mice

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Asian Journal of Andrology 2017年第6期19卷 659-665页

作者： Juri Jeong Sora Jin Heejin choi Jun tae Kwon Jihye Kim Jaehwan Kim Zee Yong Park Chunghee Cho School of Life Sciences Gwangju Institute of Science and Technology Gwangju 61005 South Korea.

Male germ cell development is a well-defined process occurring in numerous seminiferous tubules of the testis. Uncovering testicular novel genes related to intrinsic regulation of spermatogenesis is essential for the understanding of spermatogenesis. In the present study, we investigated mouse Mageg2, which belongs to a group of melanoma-associated antigens （MAGEs）. Mageg2 is transcribed in the testis specifically, and its expression level is increased at the pachytene spermatocyte stage, indicating that Mageg2 is expressed predominantly in germ cells. We generated an antibody against mouse MAGEG2 for further characterization at the protein level. Immunoblot analysis suggested that MAGEG2 has specific testicular expression and the expression primarily occurred in pachytene spermatocytes. Proteomic analyses demonstrated that mouse MAGEG2 binded to testicular germ cell-specific serine/threonine-protein kinase 31 （STK31） and heat shock protein 9 （HSPA9）. Direct binding with both interaction partners was confirmed by co-immunoprecipitation. We found that STK31 and HSPA9 bind MAGEG2 directly but not with each other. Interestingly, MAGEG2 reduced the kinase activity of STK31. Our study suggests that mouse MAGEG2 has at least two functions, including chaperone activity related to HSPA9 and regulation of pachytene spermatocyte-specific kinase, STK31. Altogether, our results provide the first information about MAGEG2 at the transcript and protein levels and suggest its potential molecular functions.

关键词： heat shock proteins serine/threonine kinase spermatogenesis testis

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