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Claudin-1 Leads to Strong Formation of Tight Junction in Cultured Mouse Lung Microvascular Endothelial Cells

Pharmacology & Pharmacy 2016年第3期7卷 133-139页

作者： Yukari Ueda Yasuhumi Shinmyouzu Hikaru Nakayama tadatoshi tanino Eiko Sakurai Eiichi Sakurai Department of Pharmaceutics Faculty of Pharmaceutical Sciences Tokushima Bunri University Tokushima Japan Faculty of Pharmacy Iwaki Meisei University Fukushima Japan

We aimed to examine paracellular barrier function in cultured mouse lung microvascular endothelial cells (LMECs). The transcellular resistance of LMEC monolayers yielded an electrical resistance of approximately 19 Ω × cm2 at days 6 - 7 in culture when the cells reached confluence, and paracellular permeable clearance of sodium fluorescein was the lowest on day 6 in culture, suggesting the formation of tight junctions (TJs) in cultured LMECs. Moreover, the expression of TJ-associated proteins, occludin, claudin-1 and -4 and zonula occludents 1 (ZO-1) was detected in LMECs at day 6 in culture. However, mRNAs of occludin, claudin-1 and -4 and ZO-1 were already expressed on day 1 after culture, and large variations were absent in the mRNA levels of occludin, claudin-4 and ZO-1 between days 1 and 7 in culture, when the level of each mRNA on day 1 in culture was used as a basal level. However, the claudin-1 mRNA level gradually increased up to approximately 7-fold on day 7 in culture over the basal level. These results indicate that the drastic increase in the mRNA expression level of claudin-1 leads to the strong formation of TJs.

关键词： Mouse Lung Microvascular Endothelial Cells Paracellular Permeability Tight Junction Occludin Claudins Zonula Occludents

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