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Amelanotic malignant melanoma of the esophagus:Report of two cases with immunohistochemical and molecular genetic study of KIT and PDGFRA

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World Journal of Gastroenterology 2009年第21期15卷 2679-2683页

作者： tadashi terada Department of Pathology Shizuoka City Shimizu Hospital

The author reports herein two cases of amelanotic malignant melanoma of the esophagus. Case 1 is an 87-year-old woman who was admitted to our hospital because of nausea and vomiting. Endoscopic examination revealed an ulcerated tumor of the distal esophagus, and a biopsy was taken. The biopsy showed malignant polygonal and spindle cells. No melanin pigment was recognized. Immunohistochemically, the tumor cells were positive for melanosome （HMB45）, S100 protein, KIT and Platelet derived growth factor receptor-α （PDG- FRA）. The patient was treated by chemotherapy and radiation, but died of systemic metastasis 12 mo after the presentation. Case 2 is a 56-year-old man presenting with dysphagia. Endoscopic examination revealed a polypoid tumor in the middle esophagus, and a biopsy was obtained. The biopsy showed malignant spindle cells without melanin pigment. Immunohistochemically, the tumor cells were positively labeled for melanosome,S100 protein, KIT and PDGFRA. The patient refused operation, and was treated by palliative chemotherapy and radiation. He died of metastasis 7 mo aEer the admission. In both cases, molecular genetic analyses of gene （exons 9, 11, 13 and 17） and PDGFRA gene （exons 12 and 18） were performed by the PCR direct sequencing method, which showed no mutations of KIT and PDGFRA genes. This is the first report of esophageal malignant melanoma with an examination of the expression of KIT and PDGFRA and the mutational status of K/T and PDGFRA genes.

关键词： Esophagus Amelanotic melanoma KIT Platelet derived growth factor receptor-c~

Primary multiple extragastrointestinal stromal tumors of the omentum with different mutations of c-kit gene

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World Journal of Gastroenterology 2008年第47期14卷 7256-7259页

作者： tadashi terada Department of Pathology Shizuoka Municipal Shimizu HospitalMiyakami 1231Shimizu-Ku424-8636ShizuokaJapan

The author reports a very rare case of sporadic primary multiple extragastrointestinal stromal tumors (EGISTs) of the omentum associated with different mutations of the exon 11 of the c-kit gene in a 75-year-old man with gastric cancer. During an operation for the cancer, two solid tumors (10 mm and 8 mm) were found in the omentum. Both tumors consisted of cellular spindle cells. Mitotic figures were two and three per 50 high power fields. The tumor cells were positive for KIT, CD34 and vimentin, but negative for desmin, S100 protein, α-smooth muscle actin and p53 protein. Ki67 labeling was 2% and 3%. The larger EGIST showed a deletion of codons 552-558 of exon 11 of the c-kit gene, while the smaller EGIST had a point mutation at codon 559 (GTT←GAT) in exon 11 of the c-kit gene. Exons 9, 13, and 17 of the c-kit gene, and exons 12 and 18 of the platelet derived growth factor receptor α genes showed no mutations. The case shows that sporadic multiple EGISTs can occur in the omentum.

关键词： Omentum Extragastrointestinal stromal tumors c-kit Platelet derived growth factor receptor CD34

Histopathological study using computer database of 10000 consecutive gastric specimens:(2)malignant lesions

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Gastroenterology Report 2016年第1期4卷 54-58,I0003页

作者： tadashi terada Department of Pathology Shizuoka City Shimizu HospitalShizuokaJapan

Using a computer database,the author investigated the histopathology of 10000 consecutive gastric specimens collected in the last 12 years 2002–2013 at his pathology laboratory in a relatively large hospital in *** of histological sections was done when *** gastric specimens were made up of 8579 benign conditions and 1421 malignant *** latter comprised gastric carcinoma in 1342 cases(94.4%),gastrointestinal stromal tumor(GIST)in 34(2.4%),mucosal-associated lymphoid tissue(MALT)lymphoma in 25(1.8%),non-Hodgkin’s malignant lymphoma in 19(1.3%),and metastatic carcinoma in 1 case(0.1%).Of the 1342 cases of gastric carcinoma,the histological type was as follows:tubular adenocarcinoma in 755 cases,papillary adenocarcinoma in 176,mucinous adenocarcinoma in 147,signet ring cell carcinoma in 145,poorly differentiated adenocarcinoma in 114,adenosquamous carcinoma in 4,and metastatic small cell carcinoma from the lung in *** surgically resected cases,the number of early gastric carcinomas was 258 and of advanced carcinoma,521 *** GIST(n=34),there were 2 cases of the epithelioid type and 32 of the spindle cell *** size of GIST ranged from 1–15 cm,with a mean of 5.6 ***(CD117)was positive in 34/34 cases,CD34 in 31/34,desmin 2/34,and S1004/34.A genetic analysis was performed in 6 cases of GIST,all of which showed point mutation of KIT and/or PDGFRA *** MALT lymphoma(n=25),centrocyte-like cells and lymphoepithelial lesions were seen in every *** pylori infection was noted in 92%.In non-Hodgkin’s lymphoma(n=19),17 cases were of diffuse large B-cell lymphoma,and 1 was peripheral T-cell lymphoma,while 1 was NK-cell lymphoma.

关键词： stomach malignant lesions histopathology

Esophageal combined carcinomas:Immunohoistochemical and molecular genetic studies

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World Journal of Gastroenterology 2012年第13期18卷 1545-1551页

作者： tadashi terada Hirotoshi Maruo Department of Pathology Shizuoka City Shimizu HospitalShizuoka 424-8636Japan Department of Surgery Shizuoka City Shimizu Hospital424-8636 izuokaJapan

Primary esophageal combined carcinoma is very rare. The authors herein report 2 cases. Case 1 was a com- bined squamous cell carcinoma and small cell carci- noma, and case 2 was a combined squamous cell carci- noma, adenocarcinoma, and small cell carcinoma. Case 1 was a 67-year-old man with complaints of dysphagia. Endoscopic examination revealed an ulcerated tumor in the middle esophagus, and 6 biopsies were obtained. All 6 biopsies revealed a mixture of squamous cell car- cinoma and small cell carcinoma. Both elements were positive for cytokeratin, epithelial membrane antigen, and p53 protein, and had high Ki-67 labeling. The small cell carcinoma element was positive for synaptophysin, CD56, KIT, and platelet-derived growth factor-~ （PDG- FRA）, while the squamous cell carcinoma element was not. Genetically, no mutations of K/Tand PDGFRA were recognized. The patient died of systemic carcinomato- sis 15 mo after presentation. Case 2 was a 74-year-old man presenting with dysplasia. Endoscopy revealed a polypoid tumor in the distal esophagus. Seven biopsies were taken, and 6 showed a mixture of squamous cell carcinoma, small cell carcinoma, and adenocarcinoma. The 3 elements were positive for cytokeratins, epithe-lial membrane antigen, and p53 protein, and had high Ki-67 labeling. The adenocarcinoma element was posi- tive for mucins. The small cell carcinoma element was positive for CD56, synaptophysin, KIT, and PDGFRA, but the other elements were not. Mutations of KIT and PDGFRA were not recognized. The patient died of sys- temic carcinomatosis 7 mo after presentation. These combined carcinomas may arise from enterochromaf- fin cells or totipotential stem cell in the esophagus or transdifferentiation of one element to another. A review of the literature was performed.

关键词： Esophagus Combined carcinoma Histopath-ology Immunohistochemistry Molecular genetics

Histopathological study using computer database of 10000 consecutive gastric specimens:(1)benign conditions

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Gastroenterology Report 2015年第3期3卷 238-242页

作者： tadashi terada Department of Pathology Shizuoka City Shimizu HospitalShizuokaJapan

Using a computer database,the author investigated the histopathology of 10000 consecutive gastric specimens,taken in the last 12 years(2002-2013)at the pathology laboratory of a Japanese ***-observation of the already examined histological sections was done when the histological diagnosis and findings on the computer data base were not very *** gastric specimens were identified as 8579 benign conditions and 1421 malignant *** 8579 benign conditions were comprised almost normal stomach in 74 cases(0.9%),chronic gastritis in 4374(51.0%),benign gastric peptic ulcer in 2195(25.6%),foveolar hyperplastic polyp in 1004(11.7%),fundic gland polyp in 421(4.9%),adenoma in 487(5.6%),heterotopic pancreas in 9(0.1%),pancreatic acinar metaplasia(PAM)in 8(0.1%),and amyloidosis in 7(0.1%).Chronic gastritis showed lymphocytic infiltration and frequently showed erosions and intestinal *** peptic ulcer showed exudate,necrosis,active inflammation,and regenerative atypia of the *** hyperplastic polyp revealed 23 malignant changes and frequently showed dysplastic glands and intestinal *** gland polyp demonstrated cystic dilations of fundic gland *** adenoma showed adenomatous proliferation in the superficial mucosa and cystic dilation of the epitheliumunder the *** pancreas was located in the submucosa and consisted of acinar cells,ducts,and occasionally *** was a tiny lesion in the mucosa and consisted of only pancreatic acinar *** was primary amyloidosis with positive reaction with Congo-red stain.

关键词： stomach benign lesions histopathology

Simultaneous large cell neuroendocrine carcinoma and adenocarcinoma of the stomach

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World Journal of Gastroenterology 2011年第43期17卷 4831-4834页

作者： tadashi terada Hirotoshi Maruo Department of Pathology Shizuoka City Shimizu Hospital Department of Surgery Shizuoka City Shimizu Hospital

A large cell neuroendocrine carcinoma(LCNEC) of the stomach is very rare.A 76-year-old Japanese man was admitted to our hospital because of epigastralgia and *** revealed 2 large tumors in the *** did not have multiple endocrine neoplasia typeⅠor Zollinger-Ellison *** modalities,including computed tomography and magnetic resonance imaging,revealed no other ***,cholecystectomy,and lymph node dissection were *** resected stomach had 2 tumors:one was an antral ulcerated type 3 tumor measuring 5 cm x 5 cm,and the other was a polypoid type 1 tumor measuring 6 cm x 6 cm x 3 cm in the ***,the antral ulcerated tumor was a well differentiated adenocarcinoma with deep *** fundus polypoid tumor was a LCNEC,being composed of malignant large cells arranged in trabecular and nested *** tumor cells were large and the nuclei were *** were frequently present,and there were many mitotic figures,apoptotic bodies,and necrotic *** lymphovascular permeation was *** out of 29 dissected lymph nodes showed metastatic foci;6 were from the LCNEC and 1 from *** intravascular tumor emboli of LCNEC were seen in the peritoneum around the lymph *** were present in the adenocarcinoma but not in the ***,the LCNEC tumor cells were positive for pancytokeratins,synaptophysin(50%positive) ,chromogranin A(10% positive) ,Ki-67(90%labeled) ,and platelet-derived growth factor-α(80%positive) .They were negative for KIT,p53,CD56,and neuron-specific *** non-cancerous stomach showed a normal number of endocrine *** patient is now treated with adjuvant chemotherapy.

关键词： Stomach Carcinomas

Intramanchette transport during primate spermiogenesis： expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis

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Asian Journal of Andrology 2008年第4期10卷 561-568页

作者： Shinichi Hayasaka Yukihiro terada Kichiya Suzuki Haruo Murakawa Ikuo Tachibana tadashi Sankai Takashi Murakami Nobuo Yaegashi Kunihiro Okamura Department of Obstetrics and Gynecology Tohoku University School of Medicine Sendai Miyagi 980-8574 Japan Suzuki Memorial Hospital lwanuma Miyagi 989-2481 Japan Tsukuba Primate Research Center National Institute of Biomedical Innovation Tukuba Ibaraki 305-0843 Japan

Aim： To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein （MyRIP）, and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport （IMT）. Methods： Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey （Macacafascieularis）. Immunocytochemical detection and reverse transcription-polymerase chain reaction （RT-PCR） analysis of the proteins were carried out. Samples were analyzed by light microscope. Results： Using RT-PCR, we found that dynein, myosin Va, MyRIP and Rab27b were expressed in monkey testis. These proteins were localized to the manchette, as shown by immunofluorescence, particularly during human and monkey spermiogenesis. Conclusion： We speculate that during primate spermiogenesis, those proteins that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport.

关键词： intramanchette transport manchette spermiogenesis