检索结果-南通市图书馆

Journal of Biomedical Science and Engineering 2022年第2期15卷 83-88页

作者： tadashi Miura Yukari Oda Yasuhiko Shizawa Oral Health Science Center Tokyo Dental College Tokyo Japan Department of Oral and Maxillofacial Implantology Tokyo Dental College Tokyo Japan Department of Liberal Arts College of Bioresource Sciences Nihon University Kanagawa Japan

Newly synthesized membrane and secretory proteins in cells undergo folding in the endoplasmic reticulum with the introduction of disulfide bonds and acquire the correct three-dimensional structure. Disulfide bonds are especially important for protein folding. It has been thought that formation of protein disulfide bonds in eukaryotes is mainly carried out by an enzyme called protein disulfide isomerase. Proteins, bearing the C-terminus of amino acids sequences with His-Asp-Glu-Leu (HDEL) sequence in yeast, in the endoplasmic reticulum (ER), which is a eukaryotic cellular organelle involved in protein synthesis, processing, and transport, have been considered to recycle between ER and Golgi apparatus. The proposal for this recycling model derives from the study of an HDEL-tagged fusion protein. Here, the localization and oligosaccharide modification of protein disulfide isomerase were investigated in yeast, and showed the first direct evidence that this intrinsic ER protein transports from ER to Golgi. Results suggest that this native protein is also accessible to post-ER enzymes, and yet accumulates in the ER.

关键词： Protein Disulfide Isomerase Endoplasmic Reticulum Golgi Apparatus Oligosaccharide Modification Protein Localization

Influence evaluation of loading conditions during pressurized thermal shock transients based on thermal-hydraulics and structural analyses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Mechanical Engineering 2018年第4期13卷 563-570页

作者： Jinya KATSUYAMA Shumpei UNO tadashi WATANABE Yinsheng LI Japan Atomic Energy Agency Ibaraki 319-1195 Japan Mizuho Information & Research Institute Inc. Tokyo 101-8443 Japan Fukui University Fukui 914-0055 Japan

The thermal hydraulic （TH） behavior of coo- lant water is a key factor in the structural integrity assessments on reactor pressure vessels （RPVs） of pressurized water reactors （PWRs） under pressurized thermal shock （PTS） events, because the TH behavior may affect the loading conditions in the assessment. From the viewpoint of TH behavior, configuration of plant equipment and their dimensions, and operator action time considerably influence various parameters, such as the temperature and flow rate of coolant water and inner pressure. In this study, to investigate the influence of the operator action time on TH behavior during a PTS event, we developed an analysis model for a typical Japanese PWR plant, including the RPV and the main components of both primary and secondary systems, and performed TH analyses by using a system analysis code called RELAP5. We applied two different operator action times based on the Japanese and the United States （US） rules： Operators may act after 10 min （Japanese rules） and 30 min （the US rules） after the occurrence of PTS events. Based on the results of TH analysis with different operator action times, we also performed structural analyses for evaluating thermal-stress distributions in the RPV during PTS events as loading conditions in the structural integrity assessment. From the analysis results, it was clarified that differences in operator action times significantly affect TH behavior and loading conditions, as the Japanese rule may lead to lower stresses than that under the US rule because an earlier operator action caused lower pressure in the RPV.

关键词： structural integrity reactor pressure vessel pressurized thermal shock thermal hydraulic analysis pressurized water reactor weld residual stress

Prolonged survival in patients with hand-foot skin reaction secondary to cooperative sorafenib treatment

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2021年第32期27卷 5424-5437页

作者： Masanori Ochi Toshiro Kamoshida Masahiro Araki tadashi Ikegami Department of Gastroenterology Hitachi General HospitalIbaraki 317–0077Japan Department of Gastroenterology and Hepatology Ibaraki Prefectural Central HospitalIbaraki Cancer CenterIbaraki 309-1793Japan Division of Gastroenterology and Hepatology Tokyo Medical University Ibaraki Medical CenterIbaraki 309-1793Japan

BACKGROUND Sorafenib is an oral drug that prolongs overall survival(OS)in patients with hepatocellular carcinoma.Adverse events,including hand-foot skin reaction(HFSR),lead to permanent sorafenib discontinuation.AIM To clarify the association between interventions for adverse events and patient prognosis.METHODS We performed a retrospective,multicenter study of patients treated with sorafenib monotherapy between May 2009 and March 2018.We developed a mutual cooperation system that was initiated at the start of sorafenib treatment to effectively manage adverse events.The mutual cooperation system entailed patients receiving consultations during which pharmacists provided accurate information about sorafenib to alleviate the fear and anxiety related to adverse events.We stratified the patients into three groups:Group A,patients without HFSR but with pharmacist intervention;Group B,patients with HFSR and pharmacist interventions unreported to oncologists(nonmutual cooperation system);and Group C,patients with HFSR and pharmacist interventions known to oncologists(mutual cooperation system).OS and time to treatment failure(TTF)were evaluated using the Kaplan-Meier method.RESULTS We enrolled 134 patients(Group A,n=41;Group B,n=30;Group C,n=63).The median OS was significantly different between Groups A and C(6.2 vs 13.9 mo,p<0.01)but not between Groups A and B(6.2 vs 7.7 mo,P=0.62).Group A vs Group C was an independent OS predictor(HR,0.41;95%CI:0.25-0.66;P<0.01).In Group B alone,TTF was significantly lower and the nonadherence rate was higher(P<0.01).In addition,the Spearman’s rank correlation coefficients between OS and TTF in each group were 0.41(Group A;P<0.01),0.13(Group B;P=0.51),and 0.58(Group C;P<0.01).There was a highly significant correlation between OS and TTF in Group C.However,there was no correlation between OS and TTF in Group B.CONCLUSION The mutual cooperation system increased treatment duration and improved prognosis in patients with HFSR.Future prospective studies(e.g.,randomized controlled trials)an

关键词： Hepatocellular carcinoma Sorafenib Pharmacists Oncologists Prognosis Duration of therapy

Current clinical management of gastrointestinal stromal tumor

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第26期24卷 2806-2817页

作者： Kazuya Akahoshi Masafumi Oya tadashi Koga Yuki Shiratsuchi Department of Gastroenterology Aso Iizuka Hospital Department of Pathology Aso Iizuka Hospital Department of Surgery Aso Iizuka Hospital

Gastrointestinal stromal tumors(GISTs) are the most common malignant subepithelial lesions(SELs) of the gastrointestinal tract. They originate from the interstitial cells of Cajal located within the muscle layer and are characterized by over-expression of the tyrosine kinase receptor KIT. Pathologically, diagnosis of a GIST relies on morphology and immunohistochemistry [KIT and/or discovered on gastrointestinal stromal tumor 1(DOG1) is generally positive]. The prognosis of this disease is associated with the tumor size and mitotic index. The standard treatment of a GIST without metastasis is surgical resection. A GIST with metastasis is usually only treated by tyrosine kinase inhibitors without radical cure; thus, early diagnosis is the only way to improve its prognosis. However, a GIST is usually detected as a SEL during endoscopy, and many benign and malignant conditions may manifest as SELs. Conventional endoscopic biopsy is difficult for tumors without ulceration. Most SELs have therefore been managed without a histological diagnosis. However, a favorable prognosis of a GIST is associated with early histological diagnosis and R0 resection. Endoscopic ultrasonography(EUS) and EUS-guided fine needle aspiration(EUSFNA) are critical for an accurate diagnosis of SELs. EUSFNA is safe and effective in enabling an early histological diagnosis and adequate treatment. This review outlines the current evidence for the diagnosis and management of GISTs, with an emphasis on early management of small SELs.

关键词： Gastrointestinal stromal tumor Endoscopic ultrasonography-guided fine needle aspiration Endoscopic ultrasonography Diagnosis Therapy

Effect of Porous Copper Pore Density on Joint Interface:Microstructure and Mechanical Analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Harbin Institute of Technology(New Series) 2022年第1期29卷 24-31页

作者： Nur Amirah Mohd Zahri Farazila Yusof Yukio Miyashita tadashi Ariga ASMdAbdul Haseeb Nazatul Liana Sukiman Department of Mechanical Engineering Faculty of EngineeringUniversity of MalayaKuala Lumpur 50603Malaysia Department of Mechanical Engineering Faculty of Engineering and Advanced Manufacturing&Material Processing(AMMP Centre)University of MalayaKuala Lumpur 50603Malaysia Department of Mechanical Engineering Nagaoka University of TechnologyNagaoka 940-2188Japan Department of Metallurgical Engineering Tokai UniversityHiratsuka 259-1292Japan Department of Mechanical Engineering Faculty of Engineering and Centre of Advanced Materials(CAM)University of MalayaKuala Lumpur 50603Malaysia

The effect of the pore density of porous copper(Cu)on brazed Cu/porous Cu was investigated.A filler with a composition of Cu⁃9.0Sn⁃7.0Ni⁃6.0P(Sn:Tin;Ni:Nickel;P:Phosphorus)and porous Cu with pore densities of 15 pores per inch(PPI),25 PPI,and 50 PPI were employed.The joint strength of Cu/porous Cu was evaluated with shear tests at different brazing temperatures.Characterizations of the joint interface and fractured surface were achieved with scanning electron microscope(SEM),energy dispersive X⁃ray spectroscopy(EDX),and X⁃ray diffraction(XRD).The micro⁃hardness test of Cu/porous Cu joint interface showed a high hardness value(HV)for 50 PPI porous Cu.This result was in line with its low shear strength.It was proved that the joint strength of Cu/porous Cu is dependent on the pore density of the porous Cu structure and brittle phases of Cu_(3)P and Ni_(3)P in the brazed interface.

关键词： porous metal brazing interface microstructure shear fracture

Accelerated degradation of a variety of aromatic compounds by Spirodela polyrrhiza-bacterial associations and contribution of root exudates released from S. polyrrhiza

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2010年第4期22卷 494-499页

作者： Hai Hoang Ning Yu tadashi Toyama Daisuke Inoue Kazunari Sei Michihiko Ike Division of Sustainable Energy and Environmental Engineering Osaka University Suita Osaka 565-0871 Japan Division of Applied Sciences Muroran Institute of Technology Muroran Hokkaido 050-8585 Japan

Removal experiments of phenol, aniline, 2,4-dichlorophenol, nonylphenol and bisphenol A （BPA） using Spirodela polyrrhiza- bacterial associations revealed that all compounds but BPA underwent accelerated removal. The mechanisms differed depending on the substrates. It was found that S. polyrrhiza has a great ability to release phenolic compound-rich root exudates, and the exudates seem to stimulate bacterial degradation of a variety of aromatic compounds.

关键词： accelerated biodegradation aromatic compounds rhizosphere root exudates Spirodela polyrrhiza

The role of the CNOT1 subunit of the CCR4-NOT complex in mRNA deadenylation and cell viability

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2011年第9期2卷 755-763页

作者： Kentaro Ito Akinori Takahashi Masahiro Morita Toru Suzuki tadashi Yamamoto Division of Oncology Department of Cancer BiologyInstitute of Medical ScienceUniversity of TokyoTokyo 108-8639Japan Cell Signal Unit Okinawa Institute of Science and Technology1919-1Okinawa 904-0412Japan

The human CCR4-NOT deadenylase complex consists of at least nine enzymatic and non-enzymatic subunits.Accumulating evidence suggests that the non-enzymatic subunits are involved in the regulation of mRNA deadenylation,although their precise roles remain to be established.In this study,we addressed the function of the CNOT1 subunit by depleting its expression in HeLa cells.Flow cytometric analysis revealed that the sub G1 fraction was increased in CNOT1-depleted cells.Virtually,the same level of the sub G1 fraction was seen when cells were treated with a mixture of siRNAs targeted against all enzymatic subunits,suggesting that CNOT1 depletion induces apoptosis by destroying the CCR4-NOT-associated deadenylase activity.Further analysis revealed that CNOT1 depletion leads to a reduction in the amount of other CCR4-NOT subunits.Importantly,the specific activity of the CNOT6L immunoprecipitates-associated deadenylase from CNOT1-depleted cells was less than that from control cells.The formation of P-bodies,where mRNA decay is reported to take place,was largely suppressed in CNOT1-depleted cells.Therefore,CNOT1 has an important role in exhibiting enzymatic activity of the CCR4-NOT complex,and thus is critical in control of mRNA deadenylation and mRNA decay.We further showed that CNOT1 depletion enhanced CHOP mRNA levels and activated caspase-4,which is associated with endoplasmic reticulum ER stress-induced apoptosis.Taken together,CNOT1 depletion structurally and functionally deteriorates the CCR4-NOTcomplex and induces stabilization of mRNAs,which results in the increment of translation causing ER stress-mediated apoptosis.We conclude that CNOT1 contributes to cell viability by securing the activity of the CCR4-NOT deadenylase.

关键词： deadenylation CCR4-NOT small interfering RNA P-bodies apoptosis

Van Vleck paramagnetism of europium oxyhydroxide

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rare Earths 2015年第2期33卷 177-181页

作者： Hiroaki Samata Naoki Wada tadashi C.Ozawa Graduate School of Maritime Sciences Kobe University FukaeminamiHigashinada Kobe Hyogo 658-0022 Japan International Center for Materials Nanoarchitectonics National Institute for Materials ScienceNamiki Tsukuba Ibaraki 305-0044 Japan

Europium oxyhydroxide crystals were synthesized by the flux method. The as-grown crystals were transparent and had a plate-like shape with natural flat surfaces. The powder XRD data were refined by assuming a monoclinic structure of the space group P21/m and lattice parameters of a=0.4346 nm, b=0.3744 nm, c=0.6107 nm, and β=108.62°. The magnetic susceptibility of the EuOOH crystals exhibited typical Van Vleck temperature-independent paramagnetism below 120 K. The calculated susceptibility, based on Van Vleck＇s theory, agreed with the experimental data to some extent, with the coupling constant λ=458±10 K. The experimental results were in close agreement with the results calculated using a modified formula with λ=505±2 K and a constant term C=4.6×10^-4 emu/（mol·Oe）.

关键词： europium oxyhydroxide magnetic measurement Van Vleck paramagnetism rare earths

可相变的纳米析出相诱发亚稳低温钢的高延展性

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2023年第12期68卷 1243-1247,M0003页

作者：黎雨李伟王斌君闵娜刘文庆刘慧彬金学军 tadashi Furuhara Institute of Advanced Steels and Materials School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China School of Material Science and Engineering Shanghai Institute of TechnologyShanghai 201418China Key Laboratory for Microstructures Shanghai UniversityShanghai 200444China Baosteel Research Institute Shanghai 201900China Institute for Materials Research Tohoku UniversitySendai 980-8577Japan

纳米析出相在所有合金中都被用作额外的增强相,但大多数情况,特别是在低温环境下,纳米析出相的引入会严重降低材料的塑性和可加工性.这一强塑性倒置问题仍然亟待解决.基于此,本文发现了一种可相变的纳米析出相(TNP),通过其在变形过程的... 详细信息

纳米析出相在所有合金中都被用作额外的增强相,但大多数情况,特别是在低温环境下,纳米析出相的引入会严重降低材料的塑性和可加工性.这一强塑性倒置问题仍然亟待解决.基于此,本文发现了一种可相变的纳米析出相(TNP),通过其在变形过程的相变行为在亚稳基体中诱发了以微带和孪晶马氏体为主的多阶段变形机制.与不含TNP的低温钢相比,可持续的两阶段加工硬化能力使其在77 K下的屈服强度和均匀伸长率都能分别提高30%和65%.微带和析出相之间的交互作用不仅使微观结构得到动态细化,也将孪晶马氏体推迟至应变后期发生.此外,这一相变行为也可以缓解界面的错配应变,阻碍孪晶生长并获得更均匀的变形.这一研究结果证明了析出诱发塑性的创新机制,可为在极端环境下析出强化的应用提供新的方向.

关键词：纳米析出相加工硬化能力低温钢相变行为析出强化可加工性李晶极端环境

Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第27期23卷 4910-4919页

作者： takanobu takata tadashi ueda akiko sakasai-sakai masayoshi takeuchi department of advanced medicine medical research institutekanazawa medical university

AIM To determine the possibility that diabetes mellitus promotes pancreatic ductal adenocarcinoma via glyceraldehyde(GA)-derived advanced glycation-end products(GA-AGEs).METHODS PANC-1,a human pancreatic cancer cell line,was treated with 1-4 mmol/L GA for 24 h. The cell viability and intracellular GA-AGEs were measured by WST-8 assay and slot blotting. Moreover,immunostaining of PANC-1 cells with an anti-GA-AGE antibody was performed. Western blotting(WB) was used to analyze the molecular weight of GA-AGEs. Heat shock proteins 90α,90β,70,27 and cleaved caspase-3 were analyzed by WB. In addition,PANC-1 cells were treated with GA-AGEs-bovine serum albumin(GA-AGEs-BSA),as a model of extracellular GA-AGEs,and proliferation of PANC-1 cells was measured.RESULTS In PANC-1 cells,GA induced the production of GA-AGEs and cell death in a dose-dependent manner. PANC-1 cell viability was approximately 40% with a 2 mmol/L GA treatment and decreased to almost 0% with a 4 mmol/L GA treatment(each significant difference was P < 0.01). Cells treated with 2 and 4 mmol/L GA produced 6.4 and 21.2 μg/mg protein of GA-AGEs,respectively(P <0.05 and P < 0.01). The dose-dependent production of some high-molecular-weight(HMW) complexes of HSP90β,HSP70,and HSP27 was observed following administration of GA. We considered HMW complexes to be dimers and trimers with GA-AGEs-mediated aggregation. Cleaved caspase-3 could not be detected with WB. Furthermore,10 and 20 μg/m L GA-AGEs-BSA was 27% and 34% greater than that of control cells,respectively(P < 0.05 and P < 0.01).CONCLUSION Although intracellular GA-AGEs induce pancreatic cancer cell death,their secretion and release may promote the proliferation of other pancreatic cancer cells.

关键词：肿瘤提升导出 Glyceraldehyde 的先进 glycation 结束产品胰腺的 ductal 腺癌