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Programmable polyketide biosynthesis platform for production of aromatic compounds in yeast

Synthetic and Systems Biotechnology 2020年第1期5卷 11-18页

作者： tadas jakočiūnas Andreas K.Klitgaard Eftychia Eva Kontou Julie Bang Nielsen Emil Thomsen David Romero-Suarez Kai Blin Christopher J.Petzold Jennifer W.Gin Yaojun Tong Charlotte Held Gotfredsen Pep Charusanti Rasmus J.N.Frandsen Tilmann Weber Sang Yup Lee Michael K.Jensen Jay D.Keasling The Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs.LyngbyDenmark Joint BioEnergy institute EmeryvilleCAUSA Biological Systems&Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering&Department of Bioengineering University of California BerkeleyCAUSA Center for Synthetic Biochemistry Institute for Synthetic BiologyShenzhen Institutes for Advanced TechnologiesShenzhenChina Department of Chemistry Technical University of DenmarkKgs.LyngbyDenmark Department of Biotechnology and Biomedicine Technical University of DenmarkKgs.LyngbyDenmark Department of Chemical and Biomolecular Engineering(BK21 Plus Program) Korea Advanced Institute of Science and Technology(KAIST)291 Daehak-roYuseong-guDaejeon34141Republic of Korea

To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms,development of new,versatile,bio-based production platforms is required.Here we present a novel yeast-based platform for biosynthesis of bacterial aromatic polyketides.The platform is based on a synthetic polyketide synthase system enabling a first demonstration of bacterial aromatic polyketide biosynthesis in a eukaryotic host.

关键词： Natural products Synthetic biology Aromatic polyketides CRiSPR/Cas9 Polyketide Production platform in eukaryotes Metabolic engineering Biotechnology

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A FAiR-compliant parts catalogue for genome engineering and expression control in Saccharomyces cerevisiae

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Synthetic and Systems Biotechnology 2022年第2期7卷 657-663页

作者： Vasil D’Ambrosio Lea G.Hansen Jie Zhang Emil D.Jensen Dushica Arsovska Marcos Laloux tadas jakociunas Pernille Hjort Davide De Lucrezia Serena Marletta Jay D.Keasling Michael K.Jensen Novo Nordisk Foundation Center for Biosustainability Technical University of DenmarkKgs.LyngbyDenmark Explora Biotech Srl Italy Joint BioEnergy institute EmeryvilleCAUSA Biological Systems and Engineering Division Lawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering Department of BioengineeringUniversity of CaliforniaBerkeleyCAUSA Center for Synthetic Biochemistry Institute for Synthetic BiologyShenzhen Institutes of Advanced TechnologiesShenzhenChina

The synthetic biology toolkit for baker’s yeast,Saccharomyces cerevisiae,includes extensive genome engineering toolkits and parts repositories.However,with the increasing complexity of engineering tasks and versatile applications of this model eukaryote,there is a continued interest to expand and diversify the rational engineering capabilities in this chassis by FAiR(findable,accessible,interoperable,and reproducible)compliance.in this study,we designed and characterised 41 synthetic guide RNA sequences to expand the CRiSPR-based genome engineering capabilities for easy and efficient replacement of genomically encoded elements.Moreover,we characterize in high temporal resolution 20 native promoters and 18 terminators using fluorescein and LUDOX CL-X as references for GFP expression and OD600 measurements,respectively.Additionally,all data and reported analysis is provided in a publicly accessible jupyter notebook providing a tool for researchers with low-coding skills to further explore the generated data as well as a template for researchers to write their own scripts.We expect the data,parts,and databases associated with this study to support a FAiR-compliant resource for further advancing the engineering of yeasts.

关键词： gRNA Promoter Terminator Yeast Standardization

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通向2075之路全球经济增长放缓,但将继续趋同发展

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中国货币市场 2023年第4期 81-86页

作者： Kevin Daly tadas Gedminas 高盛全球投资研究部

高盛全球投资研究部宏观经济研究团队2023年发表关乎未来数十年全球增长预测的四个核心主题,即人口增速放缓拖累全球潜在经济增长率;在亚洲经济引擎的带领下,新兴市场的趋同趋势保持不变;美国经济独有的超预期表现在未来十年不太可能重... 详细信息

高盛全球投资研究部宏观经济研究团队2023年发表关乎未来数十年全球增长预测的四个核心主题,即人口增速放缓拖累全球潜在经济增长率;在亚洲经济引擎的带领下,新兴市场的趋同趋势保持不变;美国经济独有的超预期表现在未来十年不太可能重演;全球不平等减少,国家内部不平等增加。同时文章揭示了全球增长面临的两大主要长期风险:保护主义和气候变化。

关键词：增长预测潜在经济增长率新兴市场经济引擎核心主题保护主义气候变化高盛

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极端火灾天气是导致澳大利亚东南部森林大火的主要原因

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Science Bulletin 2022年第6期67卷 655-664,M0004页

作者：王斌 Allan C.Spessa 冯璞玉侯鑫岳超罗京佳 Philippe Ciais Cathy Waters Annette Cowie Rachael H.Nolan tadas Nikonovas Huidong Jin Henry Walshaw 魏菁华郭小伟刘德立于强 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Northwest A&F UniversityYangling 712100China New South Wales Department of Primary industries Wagga Wagga Agricultural InstituteWagga Wagga 2650Australia Department of Geography College of ScienceSwansea UniversitySingleton ParkSwansea SA28PPUK College of Land Science and Technology China Agricultural UniversityBeijing 100193China College of Natural Resources and Environment Northwest A&F UniversityYangling 712100China institute for Climate and Application Research(iCAR)/Key Laboratory of Meteorological Disaster of Ministry of Education(KLME) Nanjing University of Information Science and TechnologyNanjing 210044China Laboratoire des Sciences du Climat et de l'Environnement CEA-CNRS-UVSQGif sur Yvette F-91191France New South Wales Department of Primary industries Dubbo 2830Australia New South Wales Department of Primary industries Armidale 2351Australia School of Environmental and Rural Science University of New EnglandArmidale 2351Australia Hawkesbury institute for the Environment Western Sydney UniversityPenrith 2751Australia CSiRO Data61 Canberra 2601Australia Python Charmers Pty Ltd Hawthorn 3122Australia Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810008China Climate Change Research Centre University of New South WalesSydney 2052Australia College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China

2019年底至2020年初,澳大利亚东南部发生森林大火,燃烧面积达540万公顷,打破当地历史观测纪录,引发全球关注.此次火灾强度高,且多发在人类聚集区,对当地人类健康、社会经济以及生态系统造成重大影响.研究森林火灾的驱动因素并确定其对... 详细信息

2019年底至2020年初,澳大利亚东南部发生森林大火,燃烧面积达540万公顷,打破当地历史观测纪录,引发全球关注.此次火灾强度高,且多发在人类聚集区,对当地人类健康、社会经济以及生态系统造成重大影响.研究森林火灾的驱动因素并确定其对火灾程度影响的阈值,可有效帮助当地消防机构和管理部门为潜在火灾做好准备.本研究选取与火灾天气和植被生产力有关的环境要素,开发了一个基于数据驱动的机器学习诊断模型.该诊断模型对燃烧面积估测的准确性达80%以上.过去20年间,澳大利亚东南部森林火灾面积与环境要素之间呈非线性关系,且大火主要受极端火灾天气控制.由于全球变暖的影响,南极振荡和印度洋偶极子指数异常波动,导致该区域极端火灾天气频发.本研究开发的火灾面积诊断模型有助于改进澳大利亚森林火灾预警系统,并为有效防范火灾提供决策依据.

关键词：森林大火燃烧面积消防机构诊断模型环境要素机器学习观测纪录森林火灾

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Are invasive Genotypes Superior? An Experimental Approach Using Native and invasive Genotypes of the invasive Grass <i>Phalaris Arundinacea</i>

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Open Journal of Ecology 2017年第2期7卷 125-139页

作者： Jane Molofsky Alexandra R. Collins Eric imbert tadas Bitinas Sébastien Lavergne Department of Plant Biology University of Vermont Burlington VT USA Division of information Technology & Sciences Champlain College Burlington VT USA institut des Sciences de l’Evolution Université de Montpellier Montpellier France Departamento de Ecología Universidad de Alicante Alicante Spain Laboratoire d’Ecologie Alpine (LECA) CNRS-Université Grenoble Alpes Grenoble France

The admixture and recombination of individuals from the native range into a new range may lead to the production of invasive genotypes that have higher fitness and wider climatic tolerances than the native genotypes. in this paper, we compare the survival and growth of native EU and invasive NA genotypes when planted back into the native EU range near where the EU genotypes were collected. We test this hypothesis using the invasive wetland grass Phalaris arundinacea. if invasive genotypes have evolved to have higher survival and growth, then they should outperform the native EU genotypes under field conditions that are better suited to the EU genotypes. individual plants of the wetland grass, Phalaris arundinacea collected from native Europe (Czech Republic (CZ) and France (FR)) and North America (Vermont (VT) and North Carolina (NC)) were planted into common gardens in Trebon, Czech Republic (49.0042°N, 14.7721°E) and Moussac, France (43.9808°N, 4.2241°E). invasive genotypes from North Carolina (NC) survived as well or better than native genotypes in both the Trebon and Moussac garden. Additionally, invasive NC genotypes suffered higher herbivore damage than native genotypes but their growth and survival were not significantly different than genotypes from the other re-gions. A companion field experiment that simulated biomass removal through grazing indicated that invasive NC genotypes recovered faster following grazing than genotypes from other regions. Our results suggest that not all invasive genotypes are superior and regional differences in aggressiveness between invasive genotypes are as great as differences between individuals from native and invasive populations. introduction of genotypes leading to invasion depends upon the environmental conditions and the suitability of the climate for the introduced individuals.

关键词： invasive Plants Admixture invasive Genotypes Enemy Release Hypothesis Evolution of increased Competitive Ability Common Garden Experiment Phalaris arundinacea

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