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The initial phase of oxidative stress in a steroid-induced osteonecrosis rabbit model

Advances in Bioscience and Biotechnology 2012年第7期3卷 978-982页

作者： Toru Ichiseki Ayumi Kaneuji Yoshimichi Ueda Seiji Kaneko Syusuke Ueda tadami matsumoto Department of Orthopaedic Surgery Kanazawa Medical University Ishikawa Japan Department of Pathophysiological and Experimental Pathology Kanazawa Medical University Ishikawa Japan

Using a rabbit model, we examined N epsilon-(hexanoyl) lysine (HEL) levels in bone and urine to detect when peroxidative reaction first occurs after steroid administration. Japanese white rabbits weighing about 3.5 kg each were injected with a single intramuscular dose of methylprednisolone 40 mg/kg and divided into groups consisting of 10 rabbits each, which were killed after 1, 3, 5 and 14 days (groups A, B, C and D respectively). As a control, 10 untreated rabbits (group N) were also studied. The proximal femurs were examined histopathologically and immunohistochemically using monoclonal antibody HEL, which is a highly specific antibody against N epsilon-(hexanoyl) lysine, an early peroxidation marker. In addition, urinary levels of HEL were measured by enzyme-linked immunosorbent assay in group N, A, B and C. Osteonecrosis was detected only in group D (90%). Increase of positive reaction of HEL in the bone was observed in group A and D. HEL expression in group D was judged to be a secondary reaction resulting from the development of osteonecrosis. Urinary level of HEL showed a significant increase in only group A (P < 0.001). The present findings suggest that peroxidation in bone occurred within 24 hours after steroid administration in a rabbit model and that it is possible to noninvasively grasp the timing of this peroxidative reaction by measuring the urinary level of HEL.

关键词： Steroid Osteonecrosis Oxidative Stress Hexanoyl Lysine

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The timing and extent of intraosseous hypoxia in the oxidative stress-induced rat osteonecrosis model

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Advances in Bioscience and Biotechnology 2013年第8期4卷 814-817页

作者： Toru Ichiseki Ayumi Kaneuji Seiji Kaneko Syusuke Ueda Yoshimichi Ueda Hideto Yonekura Kiyokazu Fukui tadami matsumoto Department of Biochemistry Kanazawa Medical University Uchinada Ishikawa Japan Department of Orthopaedic Surgery Kanazawa Medical University Uchinada Ishikawa Japan Department of Pathophysiological and Experimental Pathology Kanazawa Medical University Uchinada Ishikawa Japan

Using a rat oxidative stress-induced femoral head osteonecrosis model, we determined the presence/ absence and timing of the generation of hypoxia in the femoral head. DL-Buthionine-(S,R)-sulfoximine (BSO) 500 mg/kg was administered intraperitoneally to male Wistar rats. The rats were killed at 1, 3, 6, 12 hours, and 1, 3, 5 days after BSO administration, and the bilateral femora were removed. A group not administered BSO (control group) was also studied (each group n = 5). In the femoral heads of each group, the expression of hypoxia-inducible factor-1 alpha (HIF-1α) as an index of hypoxia was confirmed by the Western blot method, and quantified using analytical software. In the femoral head increased HIF-1α expression was found in all groups from 1 hour after BSO administration (p < 0.05). In particular, in all specimens of the group 3 hours after BSO administration the most intense expression of HIF-1α amounting to about 13-fold of that of control group was noted (p < 0.001). The present results suggested that in the extremely short period of 3 hours after BSO administration hypoxia severe enough to cause osteonecrosis was induced by oxidative stress in the rat femoral head.

关键词： Osteonecrosis Oxidative Stress Buthionine Sulfoximine Hypoxia-Inducible Factor-1α

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ELECTRON ENERGY DEPENDENCE OF X-RAY ENERGY DISPERSIVE SPECTROSCOPY AND ELECTRON ENERGY LOSS SPECTROSCOPY

ELECTRON ENERGY DEPENDENCE OF X-RAY ENERGY DISPERSIVE SPECTR...

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The Third Chinese-Japanese Electron Microscopy Seminar

作者： Tetsuo Oikawa Seiichi Suzuki tadami Taoka JEOL LTD .1418 NakagamiAkishimaTokyo 196Japan

*** Analytical electron microscopy for microcharacterization is now well known as a powerful technique in both basic and applied materials scinece.)There are,however,several limitations in analytical microscopy in lower voltage(100～200 kV)microscopes because of most notable multiple scattering of electrons in ***, essential advantages of higher voltage analtytical microscopy has been

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超高压电子显微镜在金属材料性能研究中的应用

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金属学报 1981年第3期 307-351页

作者：田岡忠美邹本三日本职业训练大学基础学科

一、前言目前,电子显微镜作为研究材料性能的一个有力手段,正在开辟它独立的应用领域。这是因为电镜是能对原子排列、晶体微观结构、各种晶体缺陷进行观察的唯一手段。并且,还因为它能分析出包含缺陷的微区成分;有时,也能分析其状态。... 详细信息

一、前言目前,电子显微镜作为研究材料性能的一个有力手段,正在开辟它独立的应用领域。这是因为电镜是能对原子排列、晶体微观结构、各种晶体缺陷进行观察的唯一手段。并且,还因为它能分析出包含缺陷的微区成分;有时,也能分析其状态。但是为了得到这样高分辨率的图象,使用目前100kV(或200kV)商品电镜时,允许观察

关键词：加速电压厚度衬度电镜电子显微镜原位观察晶体薄膜超高压电子显微镜性能研究金属材料

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