检索结果-南通市图书馆

Hepatitis B virus subgenotype A1 predominates in liver disease patients from Kerala,India

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第48期19卷 9294-9306页

作者： deepak Gopalakrishnan Mark Keyter Kotacherry Trivikrama Shenoy Kondarappassery Balakumaran Leena Lakshmikanthan Thayumanavan Varghese Thomas KR Vinayakumar Charles Panackel Arun T Korah Ramesh nair Anna Kramvis Hepatitis Virus diversity Research Programme Department of Internal Medicine School of Clinical Medicine Faculty of Health Sciences University of the Witwatersrand Parktown Johannesburg 2193 South Africa department of Gastroenterology Medical College Trivandrum 695011 India Population Health and Research Institute Trivandrum 695011 India Government Rajaji Hospital Madurai Medical College Madurai 625002 India department of Gastroenterology Calicut Medical College PO Calicut 673008 India

AIM:To molecularly characterize hepatitis B virus(HBV)isolates from Kerala and to relate them to the clinical manifestation of ***:Sera and clinical data were collected from91 patients diagnosed with chronic HBV infection and HBV-related hepatocellular carcinoma(HCC).HBV from44 HCC,22 cirrhotic and 25 chronic hepatitis patients were genotyped by sequencing of the complete S region or by restriction fragment length polymorphism *** basic core promoter/precore region was *** complete surface dNA sequences were assembled and aligned manually,and then compared with the sequences of HBV of genotypes(A-J)from *** evolutionary history was inferred using the Neighbor-Joining method and the evolutionary distances computed using the Kimura 2-parameter *** was performed using 1000 *** TaqMan BS-1 probe was used to quantify HBV dNA at a lower detection limit of approximately20 IU/*** variables were compared using an independent Student’s t ***χ2test or Fisher’s exact test was used to compare categorical *** differences were considered statistically significant at Pdisease status,the predominant genotype was A(72%);95%belonging to subgenotype A1,followed by genotypes d(27%)and C(1%).HCC patients infected with subgenotype A1were significantly younger than those infected with *** A1762T/G1764A was significantly associated with HCC in both genotypes A and *** G1862T was more frequent in subgenotype A1(Pd in combination with A1762T/G1764A,it was significantly associated with HBV from HCC *** C1766T/T1768A was significantly associated with genotype A(P=0.05)and HCC(P=0.03).The preS2start codon M1T/I mutation was unique to genotype A strains(15.6%)from all disease groups and occurred at a higher frequency in isolates from HCC patients(P=0.076).A higher frequency of preS deletion mutants(33.3%)was observed in genotype A from HC

关键词： Hepatocellular carcinoma Cirrhosis Chronic hepatitis Phylogenetic analysis Genotype India

Role of cardiovascular imaging in selection of donor hearts

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Transplantation 2015年第4期5卷 348-353页

作者： Nandini nair Enrique Gongora division of Cardiology Texas Tech Health Sciences Center Memorial Cardiac and Vascular Institute

AIM:To perform a systematic review of literature on use of cardiovascular imaging in assessment of donor ***: A systematic search of current literature from January 1965 to August 2015 was performed using Pub Med and Google Scholar to investigate the different imaging modalities used to assess donor ***: Recent literature still estimates only a 32% utilization of available donor hearts in the United States. Most common imaging modality used is transthoracic echocardiography. Use of advanced imaging modalities such as 3d echocardiography, cardiac computer tomography and cardiac magnetic resonance to evaluate donor hearts is not reported in literature. This review attempts to highlight the relevant imaging modalities that can be used to assess cardiac function in a time-efficient manner. The algorithm suggested in this review would hopefully pave the way to standardized protocols that can be adopted by organ procuring organizations to increase the donor ***: Use of advanced imaging techniques for a thorough assessment of organs will likely increase the donor pool.

关键词： donor heart utilization Echocardiography Cardiovascular imaging Cardiac magnetic resonance donor heart selection Cardiac computed tomography

儿童心脏移植后的体外膜肺氧合支持:单中心队列研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国小儿急救医学 2021年第2期28卷 151-151页

作者： nair AG Sleeper LA Smoot LB 方伯梁(译) 钱素云(校) 不详首都医科大学附属北京儿童医院

目的体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)用于心脏切开术后低心排患儿,但较少用于心脏移植后。本文总结了心脏移植后接受ECMO支持患儿的预后特点。设计单中心回顾性研究。场所大型儿童心脏转诊中心。对象1995至201... 详细信息

目的体外膜肺氧合(extracorporeal membrane oxygenation,ECMO)用于心脏切开术后低心排患儿,但较少用于心脏移植后。本文总结了心脏移植后接受ECMO支持患儿的预后特点。设计单中心回顾性研究。场所大型儿童心脏转诊中心。对象1995至2016年所有接受心脏移植且插管行ECMO支持的患儿。干预措施主要预后指标为ECMO后12个月的病死率。根据早期移植失败(心脏移植后ECMO≤7 d)和晚期移植失败,分析患儿的特点及与预后的相关性。测量方法和主要结果研究期间共进行了246例心脏移植手术,其中44例患儿进行了50次ECMO。心脏移植至ECMO的中位时间为1 d(0 d~11.7年),其中28例患儿早期移植失败[1(0~2)d],20例晚期移植失败患儿共行22次ECMO[中位时间0.8年(8 d~11.7年)],其中4例患儿之前因早期移植失败亦接受过ECMO支持。26例(59%)患儿存活至出院,24例(55%)患儿ECMO后12个月仍存活,晚期移植失败患儿的存活率较低(40%比67%,P=0.02)。12个月病死率的独立危险因素包括先天性心脏病、较高的肺血管阻力指数别体表面积值(>2.2 Woods U/m^(2))和较高的肌酐值。高群体反应性抗体水平仅与晚期移植失败组12个月病死率有关。结论ECMO可有效地用于抢救心脏移植后移植功能障碍的患儿,但其早期病死率较高。与12个月内病死率相关的因素包括先天性心脏病、肾功能不全、肺血管阻力指数别体表面积值升高;在心脏移植后晚期ECMO支持患儿还包括高HLA致敏性。

关键词：体外膜肺氧合机械循环支持小儿心脏移植移植物功能障碍病死率

维普期刊数据库

肿瘤体积、厚度和T分类作为手术治疗舌鳞状细胞癌效果的预测因子

在线全文

学校读者我要写书评

暂无评论

中国口腔颌面外科杂志 2018年第5期16卷 430-430页

作者： Mair M nair d nair S 赵泽亮

背景：根据TNM分类,在同一分类中具有良好预后的浅表肿瘤与深度浸润、预后不好的肿瘤常被混淆。方法：对588例未接受过治疗的舌癌患者进行回顾研究。使用肿瘤体积和厚度作为单独的模型,二元逻辑回归用于确定淋巴结转移和包膜外扩散（EC... 详细信息

背景：根据TNM分类,在同一分类中具有良好预后的浅表肿瘤与深度浸润、预后不好的肿瘤常被混淆。方法：对588例未接受过治疗的舌癌患者进行回顾研究。使用肿瘤体积和厚度作为单独的模型,二元逻辑回归用于确定淋巴结转移和包膜外扩散（ECS）的预测价值。生成C指数以量化T分类、厚度和存活的肿瘤体积的预测准确性。

关键词：舌鳞状细胞癌 TNM分类肿瘤体积预测因子手术治疗厚度接受过治疗包膜外扩散

维普期刊数据库

Acquisition and dissemination mechanisms of CTXΦ in Vibrio cholerae : New paradigm for dif residents

在线全文

学校读者我要写书评

暂无评论

World Journal of Medical Genetics 2014年第2期4卷 27-33页

作者： Bhabatosh das G Balakrish nair Rupak K Bhadra Centre for Human Microbial Ecology Translational Health Science and Technology Institute Infectious diseases and Immunology division CSIR-Indian Institute of Chemical Biology

Vibrio cholerae(V. cholerae) genome is equipped with a number of integrative mobile genetic element(IMGE) like prophages, plasmids, transposons or genomic islands, which provides fitness factors that help the pathogen to survive in changing environmental conditions. Metagenomic analyses of clinical and environmental V. cholerae isolates revealed that dimer resolution sites(dif) harbor several structurally and functionally distinct IMGEs. All IMGEs present in the dif region exploit chromosomally encoded tyrosine recombinases, Xer C and Xer d, for integration. Integration takes place due to site-specific recombination between two specific dNA sequences; chromosomal sequence is called att B and IMGEs sequence is called att P. different IMGEs present in the att P region have different attP structure but all of them are recognized by Xer C and Xer d enzymes and mediate either reversible or irreversible integration. Cholera toxin phage(CTXΦ), a lysogenic filamentous phage carrying the cholera toxin genes ctx AB, deserves special attention because it provides V. cholerae the crucial toxin and is always present in the dif region of all epidemic cholera isolates. Therefore, understanding the mechanisms of integration and dissemination of CTXΦ, genetic and ecological factors which support CTXΦ integration as well as production of virion from chromosomally integrated phage genome and interactions of CTXΦ with other genetic elements present in the genomes of V. cholerae is important for learning more about the biology of cholera pathogen.

关键词： Vibrio cholerae Cholera toxin phage VGJΦ Plasmids,Integrative mobile genetic element Xer C Xer d dimer resolution sites att P attB

欧洲和北美的肺癌筛查经验和肺结节处理的指证

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

国际医学放射学杂志 2012年第1期 96-96页

作者： A.nair d.M.Hansell 于寰 department of Radiology Royal Brompton HospitalSydney StreetLondon SW3 6NPUK

低剂量CT扫描能否成为肺癌筛查的有效工具是目前多个随机对照试验的内容,近期公布的北美国家肺癌筛检试验(NLST)的中期结果令人鼓舞。目前欧洲也有多个有

关键词：筛选肺癌临床试验随机螺旋CT 孤立肺结节

Chemical Characterization and Cytotoxic Activity of Antarctic Macroalgae Extracts against Colorectal Cancer

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Advances in Biological Chemistry 2019年第5期9卷 167-177页

作者： Rafaele Frassini Yeslei Paulino da Silva Sidnei Moura Leonardo Zambotti Villela Aline Paternostro Martins Pio Colepicolo Mutue Toyota Fujii nair S.Yokoya Claudio Martin Pereira de Pereira Vagner Reinaldo Zingalli Bueno Pereira Joao Antonio Pegas Henriques Mariana Roesch-Ely Laboratory of Genomics Proteomics and DNA RepairUniversity of Caxias do SulCaxias do SulRSBrazil Laboratory of Biosynthetic and Natural Products of Caxias do Sul Caxias do SulRSBrazil Laboratory of Biochemistry and Molecular Biology of Algae University of Sao PauloSao PauloSPBrazil Phycologycal Research Institute of BotanySao PauloSPBrazil Laboratory of Lipidomics and Biorganic—LLipidomicaBio Federal University of PelotasPelotasRSBrazil General Hospital University of Caxias do SulCaxias do SulRSBrazil

Background/Aim: Antarctic seaweeds are considered a promising source of compounds with anticancer activity. Colorectal cancer (CRC) is one of the most incident cancers with high mortality rates worldwide. This work aimed to characterize chemically extracts of the Antarctic macroalgae Iridaea cordata, Cystosphaera jacquinotii and desmarestia anceps and to evaluate the cytotoxic effects against human colon cancer HCT 116 cell line. Materials and Methods: The extracts were obtained by depletion using an ultrasound probe and were identified by High-Performance Liquid Chromatography (HPLC) and Gas Chromatography coupled with Mass Spectrometry (GC-MS). Cell viability was determined by MTT assay. Results: Hexanic and chloroform extracts of the I. cordata and the hexanic, chloroform and methanolic extracts of d. anceps were able to inhibit growth of colorectal cancer cells in the three different incubation times (24, 48 and 72 h). Through GC analysis, 01 compounds were identified in the hexane extract and 02 compounds in the chloroform extract of the algae I. cordata. The hexane extract of d. anceps macroalgae presented 5 compounds, chloroform extract 10 and methanolic extract 3 respectively, with special highlight to fucosterol. Carotenoid analysis by HPLC identified β-carotene in all species, while zeaxanthin was present in the spectrum of I. cordata and C. jacquinotii. Fucoxanthin and violaxanthin were confirmed in the brown seaweeds C. jacquinotii and d. anceps. Conclusion: Extracts of macroalgae I. Cordata and d. anceps may be a source of therapeutic agents against CRC.

关键词： Antarctic Seaweeds Antitumor Activity Colorectal Cancer

desmarestia anceps Montagne (Phaeophyceae) against Colorectal Cancer Cells: Cytotoxic Activity and Proapoptotic Effects

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Biological Chemistry 2022年第6期12卷 228-245页

作者： Rafaele Frassini daniela Steffens Sidnei Moura Cesar Aguzzoli Aline Paternostro Martins Pio Colepicolo Mutue Toyota Fujii nair S. Yokoya Cláudio Martin Pereira de Pereira Ana Cláudia Phillipus Miriam de Barcellos Falkenberg João Antonio Pêgas Henriques Mariana Roesch-Ely Laboratory of Applied Toxicology and Bioproducts University of Caxias do Sul RS Brazil Laboratory of Biosynthetic and Natural Products of Caxias do Sul RS Brazil Area of Exact Sciences and Engineering University of Caxias do Sul RS Brazil Laboratory of Biochemistry and Molecular Biology of Algae University of S&#227 o Paulo S&#227 o Paulo SP Brazil Nucleus Research Phycology Institute of Botany S&#227 o Paulo SP Brazil Laboratory of Lipidomics and Biorganic-LipidomicaBio Federal University of Pelotas Pelotas RS Brazil Study Group on Natural and Synthetic Products (GEPRONAS) Federal University of Santa Catarina SC Brazil

Impairment of apoptosis promotes abnormal cellular proliferation and accumulation of genetic alterations. Identifying bioactive compounds extracted from seaweeds that induce apoptosis in cancer cells may be explored as new agents for cancer chemoprevention and/or chemotherapy. The present study aimed to determine the chemical composition and biological activity of the chloroform crude extract and fractions from Antarctic seaweed desmarestia anceps. The chloroform extract was obtained by three consecutive macerations and the fractionation by vacuum liquid chromatography. The chemical characterization of the extract and fractions was performed through Gas Chromatography. The cytotoxicity of the crude extract and fractions was evaluated by the MTT assay. Cell death and cell cycle evaluation after 24 hours of exposure to chloroform extract were performed by flow cytometry. A total of 48 compounds were identified. The results indicate that chloroform extract and its fractions presented cytotoxic activity against HCT 116 cell line in a dose dependent-manner. Proapoptotic events were observed after chloroform extract exposition, which promoted an increase of multinucleated cells and reduced cell viability. This study was the first to explore cytotoxic potential of seaweed d. anceps fractions against HCT colorectal cancer cell line, suggesting that these macroalgae may be a promising candidate against anticancer activity.

关键词： Antarctic Seaweeds Antitumor Activity Apoptosis Proapoptotic Effect Colorectal Cancer

从TOd到TAC：利用新公交系统重塑街道走廊

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

城市规划学刊 2022年第1期 120-121页

作者： NEWMANP dAVIES-SLATES CONLEY d 姚智远同济大学建筑与城市规划学院

1研究背景目前围绕轨道站点进行公共交通导向开发（Transit-Oriented development简称TOd）的模式已被广泛接受和采纳，并且逐渐成为城市交通更新和发展的重要手段。另一个交通和土地使用领域的当前术语是公共交通邻近地区开发（Transit...

1研究背景目前围绕轨道站点进行公共交通导向开发（Transit-Oriented development简称TOd）的模式已被广泛接受和采纳，并且逐渐成为城市交通更新和发展的重要手段。另一个交通和土地使用领域的当前术语是公共交通邻近地区开发（Transit-Adjacent development简称tad），它被《重新连接美国》（Reconnecting America）认为是“变坏了的TOd”。还有一个公共交通联合开发（Transit-Joint development简称TJd）的概念，是指将必要的公共和私营部门的发展机会结合在一起。

关键词：