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Metabolite profile comparisons between ascending and descending colon tissue in healthy adults

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World Journal of Gastroenterology 2020年第3期26卷 335-352页

作者： Bridget A Baxter Kristopher D parker Michael J Nosler Sangeeta Rao Rebecca Craig Catherine Seiler Elizabeth P Ryan Department of Environmental and Radiological Health Sciences Colorado State UniversityFort CollinsFort CollinsCO 80523United States University of Colorado Health Gastroenterology Clinic Fort CollinsCO 80524United States Department of Clinical Sciences Colorado State UniversityFort CollinsCO 80523United States Harmony Surgery Center Fort CollinsCO 80528United States Director of Clinical Operations Harmony Surgery CenterFort CollinsCO 80523United States

BACKGROUND Obesity is a risk factor for colorectal cancer,yet metabolic distinctions between healthy right and left colon tissue,before cancer is diagnosed,remains largely *** study compared right-ascending and left-descending colon tissue metabolomes to identify differences from the stool metabolome in normal weight,overweight,and obese *** To examine right and left colon tissue metabolites according to body mass index that may serve as mechanistic targets for interventions and biomarkers for colon cancer *** Global,non-targeted metabolomics was applied to assess right-ascending and left-descending colon tissue collected from healthy adults undergoing screening colonoscopies to test the hypothesis that BMI differentially impacts colon tissue metabolite *** colon tissue and stool metabolome of healthy adults(n=24)was analyzed for metabolite signatures and metabolic pathway networks implicated in progression of colorectal *** Ascending and descending colon contained 504 host,food,and microbiotaderived metabolites from normal weight,overweight and obese adults grouped according to body mass *** acids,lipids,and nucleotides were among the chemical types that further differentiated from the stool metabolite *** weight adults had 46 significantly different metabolites between ascending and descending colon tissue locations,whereas there were 37 metabolite differences in overweight and 28 metabolite differences for obese adults(P<0.05).Obese adults had trimethylamine N-oxide,endocannabinoids and monoacylglycerols with different relative abundances identified between ascending and descending *** and secondary bile acids,vitamins,and fatty acids also showed marked relative abundance differences in colon tissue from overweight/obese *** There were metabolite profile differences between right-ascending and leftdescending colon tissue in healthy *** lipids and other metabolites in

关键词： Colon Ascending Descending Metabolomics Obesity Stool

Acute hemorrhagic edema of infancy: the experience of a large tertiary pediatric center in Israel

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World Journal of Pediatrics 2017年第4期13卷 341-345页

作者： Limor parker Keren Shahar-Nissan Liat Ashkenazi-Hoffnung Liora Harel Jacob Amir Omer Trivizki Efraim Bilavsky Department of Pediatrics B Assaf Harofeh Medical CenterZriffinIsrael Departments of Pediatrics C Schneider Children's Medical CenterPetah TiqvaIsrael Sackler Faculty of Medicine Tel Aviv UniversityTel AvivIsrael

Background:Acute hemorrhagic edema of infancy (AHEI) is a rare leukocytoclastic vasculitis of the small vessels occurring at a young age and considered as a benign self-limited *** to its low prevalence,there are limited data on the presentation and complications of this ***:All computerized files of children who were hospitalized at a tertiary pediatric center due to AHEI over a 10 year period were ***,laboratory and histopathological data were ***:Twenty-six patients were included in our study,accounting for 0.7 cases per 1000 admissions of children aged 2 years or *** age was 12.9 *** than two thirds of the children had preceding symptoms compatible with a viral *** admission,all patients presented with typical findings of a rash and *** was most profound over the lower extremities (73%).Concomitant viral or bacterial infections were found in six *** biopsy was performed in six patients revealing leukocytoclastic *** children (50%) had systemic involvement including joint involvement (n=9),gastrointestinal hemorrhage (n=4),microscopic hematuria (n=1) and compartment syndrome of the limb (n=1).The latter was diagnosed in a patient with familial Mediterranean ***:Our largest data series highlighted what is known regarding clinical and histological findings in children with ***,contrary to what was previously reported,we found a higher rate of systemic *** AHEI is a rare entity,pediatricians should be familiar with its presentation,management and our reported complications.

关键词： acute hemorrhagic edema Finkelstein-Seidlmayer disease Henoch-Sch(o)nlein purpura vasculitis

Dietary mitigation of enteric methane emissions from ruminants:A review of plant tannin mitigation options

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Animal Nutrition 2020年第3期6卷 231-246页

作者： Byeng R.Min Sandra Solaiman Heidi M.Waldrip David parker Richard W.Todd David Brauer United States Department of Agriculture(USDA) Agricultural Research Service(ARS)BushlandTX79012USA Tuskegee University TuskegeeAL36088USA

Methane gas from livestock production activities is a significant source of greenhouse gas(GHG)emissions which have been shown to influence climate *** technologies offer a potential to manipulate the rumen biome through genetic selection reducing CH4 *** production may also be mitigated to varying degrees by various dietary intervention *** to reduce GHG emissions need to be developed which increase ruminant production efficiency whereas reducing production of CH4 from cattle,sheep,and *** emissions may be efficiently mitigated by manipulation of natural ruminal microbiota with various dietary interventions and animal production efficiency *** some CH4 abatement strategies have shown efficacy in vivo,more research is required to make any of these approaches pertinent to modern animal production *** objective of this review is to explain how anti-methanogenic compounds(e.g.,plant tannins)affect ruminal microbiota,reduce CH4 emission,and the effects on host ***,this review provides information relevant to understanding the impact of tannins on methanogenesis,which may provide a cost-effective means to reduce enteric CH4 production and the influence of ruminant animals on global GHG emissions.

关键词： Feed efficiency Greenhouse gas(GHG)emission Methanogenesis Tannin Ruminant

Quantifying E2F1 protein dynamics in single cells

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Quantitative Biology 2020年第1期8卷 20-30页

作者： Bernard Mathey-Prevot Bao-Tran parker Carolyn Im Cierra Hong Peng Dong Guang Yao Lingchong You Department of Pharmacology and Cancer Biology Duke University School of MedicineDurhamNC 27710USA Duke University School of Medicine DurhamNC 27710USA Molecular and Cellular Biology University of ArizonaTucsonAZ 85721USA Department of Biomedical Engineering Duke UniversityDurhamNC 27705USA Center for Genomic and Computational Biology Duke UniversityDurhamNC 27705USA Department of Molecular Genetics and Microbiology Duke University School of MedicineDurhamNC 27708USA Department of Pediatrics Duke University School of MedicineDurhamNC 27708USA University of North Carolina School of Medicine Chapel HillNC 27516USA Janelia Research Campus Howard Hughes Medical InstituteAshburnVA 20147USA

Background:E2F1 protein,a major effector of the Rb/E2F pathway plays a central role in regulating cell-fate decisions involved in proliferation,apoptosis,and *** expression is highly dynamic and tightly modulated through a combination of transcriptional,translational and posttranslational ***,the mechanisms by which its expression and activity can promote different cellular outcomes remain to be fully *** better document E2F1 expression in live cells,we have engineered a series of fluorescent E2F1 protein reporters that quantitatively capture E2F1 protein ***:Reporter constructs,under the control of the mouse or human E2F1 proximal promoter,were designed to express an E2F1-Venus fusion protein incapable of binding *** addition,constructs either included or excluded the y untranslated region(3fUTR)of the E2F1 *** constructs were introduced into fibroblasts and epithelial cells,and expression of the fusion reporter protein was validated and quantified in single cells using live ***:In all cases,expression of the reporter protein effectively recapitulated the behavior of E2F1 under various conditions,including cell cycle progression and genotoxic *** or little fluorescent signal of the reporter was detected in G〇,but as the cycle progressed,expression of the reporter protein steadily increased in the nucleus,peaking a few hours before cell division,but declining to baseline 2-3 h prior to the onset of *** absence of the E2F13 fUTR in the constructs led to considerably higher steady-state levels of the fusion protein,which although normally regulated,exhibited a slightly less complex dynamic profile during the cell cycle or genotoxic ***,the presence or absence of Rb failed to impact the overall detection and levels of the reporter ***:Our validated E2F1 protein reporters complement nicely other reporters of the Rb/E2F pathway and provide a unique tool to f

关键词： protein E2F1 reporter cell cycle

Cold Winter Warm Thoughts

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英语画刊（初三版） 2012年第7期 26-27页

作者： Mary Jessie parker

暴风雪越来越凶猛，狂风怨吼着，鹅毛般的雪花拍打着车窗。寒冷的夜，是什么温暖着我们？＂Are you OK？＂ asked *** car had slid off the country road into a snowdrift（雪堆）.A white world surrounded us--the snowdrift on my ... 详细信息

暴风雪越来越凶猛，狂风怨吼着，鹅毛般的雪花拍打着车窗。寒冷的夜，是什么温暖着我们？＂Are you OK？＂ asked *** car had slid off the country road into a snowdrift（雪堆）.A white world surrounded us--the snowdrift on my side,and the wall of blowing snow on Daddy＇s. Daddy tried to start the car. Nothing happened. ＂We＇re stuck,＂ he said. ＂Well.

关键词：中学英语课外阅读阅读材料

Study the lipidoid nanoparticle mediated genome editing protein delivery using 3D intestinal tissue model

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Bioactive Materials 2021年第11期6卷 3671-3677页

作者： Tao Yang Haobo Han Ying Chen Liu Yang Rachael parker Yamin Li David L.Kaplan Qiaobing Xu Department of Biomedical Engineering Tufts UniversityMedfordMAUSA Department of Prosthodontics Guanghua School of StomatologyHospital of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhou510055China Key Laboratory for Molecular Enzymology1nd Engineering of Ministry of Education School of Life SciencesJilin UniversityChangchun130012China

Lipid nanoparticles are promising carriers for oral drug *** bioactive cargos with intracellular targets,***-editing proteins,it is essential for the cargo and carrier to remain complexed after crossing the epithelial layer of intestine in order for the delivery system to transport the cargos inside targeted ***,limited studies have been conducted to verify the integrity of cargo/carrier nanocomplexes and their capability in facilitating cargo delivery intracellularly after the nanocomplex crossing the epithelial ***,we used a traditional 2D transwell system and a recently developed 3D tissue engineered intestine model and demonstrated the synthetic lipid nanoparticle(carrier)and protein(cargo)nanocomplexes are able to cross the epithelial layer and deliver the protein cargo inside the underneath *** found that the EC16-63 LNP efficiently encapsulated the GFP-Cre recombinase,penetrated the intestinal monolayer cells in both the 2D cell culture and 3D tissue models through temporarily interrupting the tight junctions between epithelial *** transporting across the intestinal epithelia,the EC16-63 and GFP-Cre recombinase nanocomplexes can enter the underneath cells to induce gene *** results suggest that the in vitro 3D intestinal tissue model is useful for identifying effective lipid nanoparticles for potential oral drug delivery.

关键词： Lipidoid nanoparticle Protein delivery Oral drug delivery Genome engineering 3D tissue model

The lncRNA BORG: a novel inducer of TNBC metastasis, chemoresistance, and disease recurrence

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Journal of Cancer Metastasis and Treatment 2019年第5期4卷 48-61页

作者： Alex J.Gooding Kimberly A.parker Saba Valadkhan William P.Schiemann Department of Pathology Case Western Reserve UniversityClevelandOH 44106USA Department of Pharmacology Case Western Reserve UniversityClevelandOH 44106USA Department of Molecular Biology and Microbiology Case Western Reserve UniversityClevelandOH 44106USA Case Comprehensive Cancer Center Case Western Reserve UniversityClevelandOH 44106USA

Although greater than 90% of breast cancer-related mortality can be attributed to metastases, the molecular mechanisms underpinning the dissemination of primary breast tumor cells and their ability to establish malignant lesions in distant tissues remain incompletely understood. Genomic and transcriptomic analyses identified a class of transcripts called long noncoding RNA (lncRNA), which interact both directly and indirectly with key components of gene regulatory networks to alter cell proliferation, invasion, and metastasis. We identified a pro-metastatic lncRNA BMP/OP-Responsive Gene (BORG) whose aberrant expression promotes metastatic relapse by reactivating proliferative programs in dormant disseminated tumor cells (DTCs). BORG expression is broadly and strongly induced by environmental and chemotherapeutic stresses, a transcriptional response that facilitates the survival of DTCs. Transcriptomic reprogramming in response to BORG resulted in robust signaling via survival and viability pathways, as well as decreased activation of cell death pathways. As such, BORG expression acts as a (1) marker capable of predicting which breast cancer patients are predisposed to develop secondary metastatic lesions;and (2) unique therapeutic target to maximize chemosensitivity of DTCs. Here we review the molecular and cellular factors that contribute to the pathophysiological activities of BORG during its regulation of breast cancer metastasis, chemoresistance, and disease recurrence.

关键词： BORG chemoresistance disease recurrence dormancy long noncoding RNA metastasis triple-negative breast cancer

One size does not fit all:adapt and localise for effective,proportionate and equitable responses to COVID-19 in Africa

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Family Medicine and Community Health 2021年第2期9卷 11-14页

作者： Hayley MacGregor Melissa Leach Akhona Tshangela tabitha A Hrynick Shelley Lees Eva Niederberger Melissa parker Santiago Ripoll Lorenzo Hana Rohan Megan Schmidt-Sane Olivia Tulloch Annie Wilkinson Institute of Development Studies BrightonUK Africa Centres for Disease Control and Prevention African UnionAddis AbabaEthiopia Department of Global Health and Development London School of Hygiene&Tropical MedicineLondonUK Anthrologica OxfordUK

INTRODUCTION The heterogeneous epidemiological picture for COVID-19 in Africa continues to generate *** projections raise spec-ulation about the phases and trends of SARS-CoV-2 outbreaks across the continent and how these differ from outbreaks else-where.1-4 Continental efforts drew praise in the first wave of COVID-19,and success has been linked to African experience of epidemics and to decisive leadership.

关键词： Continental Africa size

Relating environmental variables with aquatic community structure in an agricultural/urban coldwater stream

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Ecological Processes 2021年第1期10卷 481-499页

作者： Daniel T.L.Myers Richard R.Rediske James N.McNair Aaron D.parker E.Wendy Ogilvie Annis Water Resources Institute Grand Valley State University740 W Shoreline DriveMuskegonMI 49401USA. Michigan Department of Environment Great Lakesand Energy525 W Allegan StreetLansingMI 48909USA. Grand Valley Metropolitan Council 678 Front Avenue NWSuite 200Grand RapidsMI 49504USA.

Background:Urban areas are often built along large rivers and surrounded by agricultural *** may lead to small tributary streams that have agricultural headwaters and urbanized lower *** study objectives assessed are as follows:(1)landscape,geomorphic,and water quality variables that best explained variation in aquatic communities and their integrity in a stream system following this agricultural-to-urban land use gradient;(2)ways this land use gradient caused aquatic communities to differ from what would be expected for an idealized natural stream or other longitudinal gradients;and(3)whether the impacts of this land use gradient on aquatic communities would grow larger in a downstream direction through the agricultural and urban *** study area was an impaired coldwater stream in Michigan,***:Many factors structured the biological communities along the agricultural-to-urban land use *** woody debris had the strongest relationship with EPT(Ephemeroptera,Plecoptera,and Trichoptera)abundance and richness and were most common in the lower,urbanized *** streambed substrate had the strongest relationship with Diptera taxa and surface air breather macroinvertebrates and was dominant in agricultural *** community assemblage was influenced largely by stream flow and temperature regimes,while poor fish community integrity in lower urban reaches could be impacted by geomorphology and episodic urban pollution *** macroinvertebrates were most abundant in deforested,first-order agricultural headwaters,while EPT macroinvertebrate richness was the highest downstream of agricultural areas within the urban zone that had extensive forest ***:Environmental variables and aquatic communities would often not conform with what we would expect from an idealized natural *** richness improved downstream of agricultural *** shows promise for the recovery of aquatic systems using we

关键词： Land use Habitat Stream Fish Macroinvertebrate Gradient