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Zoological Research 2024年第1期45卷 215-225页

作者： Zhan-Fei Wei kai-Wen ta Nan-Nan Zhang Shan-Shan Liu Liang Meng kai-Qiang Liu Chong-Yang Cai Xiao-Tong Peng Chang-Wei Shao National Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoShandong 266071China BGI-Qingdao BGI-ShenzhenQingdaoShandong 266555China Institute of deep-Sea Science and Engineering Chinese Academy of SciencesSanyaHainan 572000China

A total of 10 specimens of Alcyonacea corals were collected at depths ranging from 905 m to 1633 m by the manned submersible Shenhai Yongshi during two cruises in the South China Sea(SCS).Based on mitochondrial genomic characteristics,morphological examination,and sclerite scanning electron microscopy,the samples were categorized into four suborders(Calcaxonia,Holaxonia,Scleraxonia,and Stolonifera),and identified as 9 possible new cold-water coral *** of GC-skew dissimilarity,phylogenetic distance,and average nucleotide identity(ANI)revealed a slow evolutionary rate for the octocoral mitochondrial *** nonsynonymous(ka)to synonymous(Ks)substitution ratio(ka/Ks)suggested that the 14 protein-coding genes(PCGs)were under purifying selection,likely due to specific deep-sea environmental *** analysis of the median ka/Ks values of five gene families and environmental factors indicated that the genes encoding cytochrome b(cyt b)and dNA mismatch repair protein(mutS)may be influenced by environmental factors in the context of deep-sea species *** study highlights the slow evolutionary pace and adaptive mechanisms of deep-sea corals.

关键词： Mitochondrial genome Alcyonacea ka/Ks evolution Environmental factors

H_(2)O对合成气火焰热释放特性及NO生成路径的化学影响

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节能技术 2024年第3期42卷 219-226页

作者：刘宇谦苏凯孔俊峰刘鹏飞贺世鹏徐顺塔刘豪国家能源集团泰州发电有限公司江苏泰州225300 华中科技大学煤燃烧国家重点实验室湖北武汉430074 烟台龙源电力技术股份有限公司山东烟台264006

利用Chemkin软件研究了H_(2)O的化学效应对不同含氢量的合成气(H_(2)/CO体积比=10∶90和50∶50)火焰温度、热释放特性及NO生成路径的影响规律。结果表明,尽管H_(2)O相比N_(2)具有更高的比热容,但是其能够直接参加化学反应,促进了OH和HO_... 详细信息

利用Chemkin软件研究了H_(2)O的化学效应对不同含氢量的合成气(H_(2)/CO体积比=10∶90和50∶50)火焰温度、热释放特性及NO生成路径的影响规律。结果表明,尽管H_(2)O相比N_(2)具有更高的比热容,但是其能够直接参加化学反应,促进了OH和HO_(2)自由基的生成,提升了H+O_(2)+H_(2)O=HO_(2)+H_(2)O、H+HO_(2)=2OH、OH+CO=H+CO_(2)、OH+H_(2)=H+H_(2)O等反应的热释放速率,导致H_(2)O的化学效应能够提升合成气火焰温度,且对低氢含量的合成气火焰温度提升作用更加明显。不论H_(2)/CO比例高低,H_(2)O的化学效应均促进了热力型NO生成,抑制了NNH路径对NO生成的贡献;在高氢含量的合成气(H_(2)/CO=50∶50)火焰中H_(2)O的化学效应促进了N_(2)O路径对NO生成,但是抑制了低氢含量的合成气(H_(2)/CO=10∶90)火焰中N_(2)O向NO的转化。

关键词：合成气火焰温度 H_(2)O化学效应热释放特性 NO生成路径

Real-world performance analysis of a novel computational method in the precision oncology of pediatric tumors

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World Journal of Pediatrics 2023年第10期19卷 992-1008页

作者： Barbara Vodicska Júlia déri dóra Tihanyi Edit Várkondi EnikőKispéter Róbert dóczi dóra Lakatos Anna dirner Mátyás Vidermann Péter Filotás Réka Szalkai-dénes István Szegedi katalin Bartyik Krisztina Míta Gábor Réka Simon Péter Hauser György Péter Csongor Kiss Miklós Garami István Peták Oncompass Medicine Hungary Kft Retek Str.34Budapest 1024Hungary division of Pediatric Hematology-Oncology Department of PediatricsFaculty of MedicineUniversity of DebrecenDebrecenHungary department of Pediatrics Albert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Onco-Hematology department Velkey LászlóPaediatric Health CentreMiskolcHungary department of Paediatrics Semmelweis UniversityBudapestHungary Onco-Hematology department Heim Pál Children’s HospitalBudapestHungary department of Pharmacology and Pharmacotherapy Semmelweis UniversityBudapestHungary department of Pharmaceutical Sciences University of Illinois at ChicagoChicagoUSA Genomate Health CambridgeMAUSA

Background The utility of routine extensive molecular profiling of pediatric tumors is a matter of debate due to the high number of genetic alterations of unknown significance or low evidence and the lack of standardized and personalized decision support *** drug assignment(ddA)is a novel computational method to prioritize treatment options by aggregating numerous evidence-based associations between multiple drivers,targets,and targeted *** has been validated to improve personalized treatment decisions based on the outcome data of adult patients treated in the SHIVA01 clinical *** aim of this study was to evaluate the utility of ddA in pediatric *** Between 2017 and 2020,103 high-risk pediatric cancer patients(d in our precision oncology program,and samples from 100 patients were eligible for further *** or blood samples were analyzed by whole-exome(WES)or targeted panel sequencing and other molecular diagnostic modalities and processed by a software system using the ddA algorithm for therapeutic decision ***,a molecular tumor board(MTB)evaluated the results to provide therapy *** Of the 100 cases with comprehensive molecular diagnostic data,88 yielded WES and 12 panel sequencing *** identified matching off-label targeted treatment options(actionability)in 72/100 cases(72%),while 57/100(57%)showed potential drug *** reached 88%(29/33)by 2020 due to the continuous updates of the evidence *** approved the clinical use of a ddA-top-listed treatment in 56 of 72 actionable cases(78%).The approved therapies had significantly higher aggregated evidence levels(AELs)than dismissed *** of WES results for targeted panels missed important mutations affecting therapy *** ddA is a promising approach to overcome challenges associated with the interpretation of extensive molecular profiling in the routine car

关键词： Computational decision support Pediatric tumors Precision oncology Tumor board

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互联网金融的系统性风险测算

统计与决策 2019年第7期35卷 158-161页

作者：塔琳北京交通大学经济管理学院北京100044

文章综合我国互联网金融的特征,构建了互联网金融系统性风险的评价指标体系,然后通过主成分分析法,测算了2011—2016年分季度的互联网金融系统性风险。结果表明,近年来我国互联网金融的系统性风险总体上处于上升趋势;同时也可以根据局... 详细信息

文章综合我国互联网金融的特征,构建了互联网金融系统性风险的评价指标体系,然后通过主成分分析法,测算了2011—2016年分季度的互联网金融系统性风险。结果表明,近年来我国互联网金融的系统性风险总体上处于上升趋势;同时也可以根据局部变化特征分为四个阶段,依次为风险上升阶段、风险下降阶段、风险抬高阶段和风险缓降阶段。从风险结构来看,互联网金融市场风险、政策监管和法律风险、顺周期风险、政府债务风险和宏观经济运行风险是重要的组成部分。

关键词：互联网金融系统性风险主成分分析

(E)-2-Benzylidenecycloalkanones XII.*Kinetic Measurement of Bovine and Human Serum Albumine Interaction with Selected Chalcones and Their Cyclic Chalcone Analogues by UV Spectrophotometry

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Spectral Analysis Review 2015年第1期3卷 1-8页

作者： Beáta Veliká Vladimíra Tomecková Krisztina Fodor Ivan Kron Pál Perjési department of Medical Chemistry Biochemistry and Clinical BiochemistryFaculty of Medicine UPJSTrieda SNP 1KosiceSlovakia Institute of Pharmaceutical Chemistry University of PécsPécsHungary

UV-VIS spectroscopic investigations of interaction of bovine and human serum albumin with selected chalcones (1) and their cyclic chalcone analogues: (E)-2-(4’-X-benzylidene-1-tetralones (3), benzosuberones (4) with dimethylamino and methoxy substituents and (E)-2-(2’,4’-dimethox- ybenzylidene)-1-indanone (2) were performed in polar respiration medium. Absorption maxima of the tested compounds were investigated in the presence of bovine and human serum albumin at the 0, 10, 30 and 60 minute timepoints of the interaction. The absorbance of all studied compounds in the presence of proteins decreased after one hour of the reaction. Molecule 4a showed the strongest and fastest kinet initial interaction with both albumins.

关键词： Chalcones Cyclic Chalcone Analogues UV Spectra Kinetic Measurements Binding Constant Bovine Serum Albumin Human Serum Albumin

This work was supported by the European Research Council Advanced Grant(EtaP-742985 to *** J.F.)and by the Ministry of Science and Technology(grant 110-2636-B-005-001 to K.-J.L.).

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Plant Communications 2024年第1期5卷 130-147页

作者： Yu Zhang Yuan Fu Wenfei Xian Xiuli Li Yong Feng Fengjiao Bu Yan Shi Shiyu Chen Robin van Velzen kai Battenberg Alison M.Berry Marco G.Salgado Hui Liu Tingshuang Yi Pascale Fournier Nicole Alloisio Petar Pujic Hasna Boubakri M.Eric Schranz Pierre-Marc delaux Gane ka-Shu Wong Valerie Hocher Sergio Svistoonoff Hassen Gherbi Ertao Wang Wouter Kohlen Luis G.Wall Martin Parniske katharina Pawlowski Philippe Normand Jeffrey J.doyle Shifeng Cheng Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China Biosystematics Group Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands department of Plant Sciences University of CaliforniaDavisDavisCA 95616USA department of Ecology Environment and Plant SciencesStockholm University10691 StockholmSweden Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesLanhei RoadKunming 650201China Universite´de Lyon Universite´Lyon 1CNRSUMR5557Ecologie MicrobienneINRAUMR 141843 bd du 11 novembre 191869622 VilleurbanneFrance Laboratoire de Recherche en Sciences Ve´ge´tales(LRSV) Universite´de ToulouseCNRSUPS24 chemin de Borde RougeAuzevilleBP4261731326 Castanet TolosanFrance department of Biological Sciences University of AlbertaEdmontonAB T6G 2E9Canada French National Research Institute for Sustainable development(IRd) UMR LSTM(IRD/CIRAD/INRAe/Montpellier University/Supagro)-Campus International BaillarguetTA A-82/J34398 Montpellier Cedex 5France National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghaiChina Laboratory of Molecular Biology Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands Laboratory of Biochemistry Microbiology and Soil Biological InteractionsDepartment of Science and TechnologyNational University of QuilmesCONICETBernalArgentina Faculty of Biology GeneticsLMU MunichGroßhaderner Strasse 2-482152 MartinsriedGermany School of Integrative Plant Science Sections of Plant Biology and Plant Breeding&GeneticsCornell UniversityIthacaNY 14853USA State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China Univer

Plant root-nodule symbiosis(RNS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the Nitrogen-Fixing Nodulation Clade(NFNC),and is best understood in the legume *** species share many commonalities,explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time ***,comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for Plant Communications Genomic landscape of nodulation variation in the ***,much remains to be learned about nodulation,especially outside of ***,we employed a large-scale phylogenomic analysis across 88 species,complemented by 151 RNA-seq libraries,to elucidate the evolution of *** phylogenomic analyses further emphasize the uniqueness of the transcription factor NIN as a master regulator of nodulation and identify key muta-tions that affect its function across the *** transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants,while also identifying nodule-specific and nitrogen-response *** 70%of symbiosis-related genes are highly conserved in the four representative species,whereas defense-related and host-range restriction genes tend to be lineage *** study also identified over 900000 conserved non-coding elements(CNEs),over 300000 of which are unique to sampled NFNC ***-specific CNEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions,thus representing a pool of candidate regula-tory elements for genes involved in ***,our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RNS traits in agriculturally important crops.

关键词： nitrogen-fixing root-nodule symbiosis two competing hypotheses phylogenomics phylotranscrip-tomics conserved non-coding elements convergence deep homology

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区块链在互联网金融征信领域的应用前景探析

东北大学学报（社会科学版） 2018年第5期20卷 466-474页

作者：塔琳李孟刚北京交通大学经济管理学院北京100044

从我国互联网金融平台征信发展现状出发,分析了互联网金融平台征信存在的问题和面临的挑战。为解决现存问题建立了一个基于区块链的跨平台征信数据共享模型,探讨了其作用机理和应用场景,实证结果表明:征信系统中的机构数量与逾期率呈现... 详细信息

从我国互联网金融平台征信发展现状出发,分析了互联网金融平台征信存在的问题和面临的挑战。为解决现存问题建立了一个基于区块链的跨平台征信数据共享模型,探讨了其作用机理和应用场景,实证结果表明:征信系统中的机构数量与逾期率呈现明显的负向关系。利用区块链技术将信用平台打通后,可以大大增加系统中提供征信服务的机构数量,按照统计结果推测,项目逾期率会相应降低,有利于互联网金融行业的健康有序发展和政府监管功能的实现。区块链在一定程度上解决了现存问题,但融入征信体系仍需时日,长期来看离不开政府部门的监督。

关键词：区块链互联网金融征信政府监管

diabetes and cognitive function:An evidence-based current perspective

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World Journal of diabetes 2023年第2期14卷 92-109页

作者： Meghna Julian Sebastian Shahanas ka Khan Joseph M Pappachan Mohammad Sadiq Jeeyavudeen School of Life Sciences Keele UniversityStoke-on-Trent ST55BGUnited Kingdom department of Endocrinology and Metabolism Lancashire Teaching Hospitals NHS TrustPreston PR29HTUnited Kingdom Faculty of Science Manchester Metropolitan UniversityManchester M156BHUnited Kingdom Faculty of Biology Medicine and HealthThe University of ManchesterManchester M139PLUnited Kingdom department of Endocrinology and Metabolism University Hospitals of EdinburghEdinburgh EH164SAUnited Kingdom

Several epidemiological studies have clearly identified diabetes mellitus(dM)as a major risk factor for cognitive dysfunction,and it is going to be a major public health issue in the coming years because of the alarming rise in diabetes prevalence across the *** and neural tissues predominantly depend on glucose as energy substrate and hence,any alterations in carbohydrate metabolism can directly impact on cerebral functional output including cognition,executive capacity,and *** affects neuronal function and mental capacity in several ways,some of which include hypoperfusion of the brain tissues from cerebrovascular disease,diabetes-related alterations of glucose transporters causing abnormalities in neuronal glucose uptake and metabolism,local hyper-and hypometabolism of brain areas from insulin resistance,and recurrent hypoglycemic episodes inherent to pharmacotherapy of diabetes resulting in neuronal *** decline can further worsen diabetes care as dM is a disease largely self-managed by ***,it is crucial to understand the pathobiology of cognitive dysfunction in relation to dM and its management for optimal long-term care plan for patients.A thorough appraisal of normal metabolic characteristics of the brain,how alterations in neural metabolism affects cognition,the diagnostic algorithm for patients with diabetes and dementia,and the management and prognosis of patients when they have this dangerous combination of illnesses is imperative in this *** evidence-based narrative with the backup of latest clinical trial reviews elaborates the current understanding on diabetes and cognitive function to empower physicians to manage their patients in day-to-day clinical practice.

关键词： diabetes mellitus dementia Cognitive function Antidiabetic medications Hyperglycemia Hypoglycemia

Investigation of Interaction of Some Chalcones and Cyclic Chalcone Analogues with Outer Mitochondrial Membrane by UV-VIS and Fluorescence Spectroscopy

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Spectral Analysis Review 2013年第1期1卷 1-9页

作者： Vladimíra Tomecková Miroslava Stefanisinová Beáta Veliká Krisztina Fodor Pal Perjési Marek Stupák Juraj Guzy Stefan Tóth Jr Tímea Pekárová department of Medical Chemistry Biochemistry and Clinical BiochemistryFaculty of MedicinePavol Jozef Safárik University in KosiceKosiceSlovakia Institute of Pharmaceutical Chemistry Faculty of MedicineUniversity of PécsPécsHungary

Interaction of the synthetic chalcones (1b,1c) and their cyclic analogues (2b,2c) with bovine (BSA) and human serum albumin (HSA) as well as with rat liver mitochondria (RLM) was studied by fluorescence spectroscopy. The maxima of emission fluorescence spectra were changed only in the case of 2b and 2c during interaction with BSA, HSA as well as mitochondrial outer membrane showing a slight hypsochromic shift and decrease of fluorescence. Interaction of the methoxy-(1b,2b) and the dimethylamino-substituted (1c,2c) compounds with outer mitochondrial membrane were studied by fluorescence polarization. Fluorescence polarization of 1b in the presence of the two proteins and mitochondria was found to be unchanged. Under similar conditions (2b,1c,2c) showed continuously increasing fluorescence polarization signal during the 30 minute period of investigations. Since fluorescence polarization supposes that as a result of binding these substances to proteins and lipids. Compound 2c displayed a continuous increase of fluorescence polarization signal in the presence of proteins (BSA, HSA), yeast cytoplasm (YC) and mitochondria (YM and RLM). This compound displayed a significant cytotoxic effect. This pattern of interaction with proteins might be one of the contributing vectors of the observed cytotoxicity against several human carcinoma cell lines.

关键词： Chalcones Yeast Cytoplasm Yeast Mitochondria Rat Liver Mitochondria Fluorescence Polarization? Fluorescence Synchronous Fingerprint

猪流行性腹泻病毒S2基因B细胞表位嵌入型S-层蛋白在副干酪乳杆菌中的表达与鉴定

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中国畜牧兽医 2022年第10期49卷 4004-4011页

作者：白伟琴卡楚拉乌志勇苗苗塔娜格日勒图内蒙古农业大学兽医学院呼和浩特010010

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEdV)乳酸菌活菌疫苗载体。【方法】应用dNA重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEdV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达... 详细信息

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEdV)乳酸菌活菌疫苗载体。【方法】应用dNA重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEdV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达载体pTRK892中,构建重组载体pTRK-SLP-EpitopeS2,通过电转化方法将重组质粒导入副干酪乳杆菌中,获得重组副干酪乳杆菌。分别用SdS-PAGE、Western blotting和免疫荧光试验(IFA)鉴定目的蛋白在副干酪乳杆菌中的表达。【结果】PCR结果显示,成功扩增出大小为1400 bp的目的条带,与插入融合基因大小一致,双酶切结果出现大小分别为1400和4700 bp的2条带,基因测序结果显示无碱基缺失和突变等,从而确定重组质粒pTRK-SLP-EpitopeS2构建正确。SdS-PAGE、Western blotting结果显示,在48 ku处出现与理论值大小一致的目的蛋白条带,表明融合基因SLP-EpitopeS2在副干酪乳杆菌中得到有效表达。IFA结果显示,与对照组副干酪乳杆菌相比,重组副干酪乳杆菌均能被激发出特异性绿色荧光信号,与高浓度氯化锂(LiCl)洗脱下来的菌体膜蛋白样品补充鉴定试验结果相吻合。表明融合蛋白SLP-EpitopeS2可能在副干酪乳杆菌的菌体表面表达。【结论】成功构建了PEdV EpitopeS2及嗜酸乳杆菌SLP嵌入型融合表达载体pTRK-SLP-EpitopeS2,为乳杆菌活菌载体疫苗相关研究奠定了基础。

关键词：猪流行性腹泻病毒(PEdV) S2基因副干酪乳杆菌表达鉴定