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Transcriptome approach to understand the potential mechanisms of resistant and susceptible alfalfa(Medicago sativa L.) cultivars in response to aphid feeding

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Journal of Integrative Agriculture 2018年第11期17卷 2518-2527页

作者： tu Xiong-bing ZHAO Hai-long ZHANG Ze-hua State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Plant Protection Shenyang Agricultural University

Plant breeding for resistance to agricultural pests is an essential element in the development of integrated crop management systems, however, the molecular and genetic mechanisms underlying resistance are poorly understood. In this pilot study, we conducted a transcriptomic analysis of a resistant （R） and susceptible （S） variety of alfalfa, with （＋A） or without （-A） aphids （totally four treatments）. We used the resistant cultivar Zhongmu t and the susceptible cultivar Soca. A total of 3 549 mRNAs were differentially expressed, of which 1 738 up-regulated and 1 307 down-regulated genes were identified in S＋A/S-A plants, while 543 up-regulated and 331 down-regulated genes were identified in the R＋A/R-A plants. KEGG analysis mapped 112 and 546 differentially expressed genes to 8 and 17 substantially enriched pathways for Zhongmu 1 and Soca, respectively. Six shared pathways were linked to plant resistance traits, including phenylpropanoid biosynthesis associated with salicylic acid synthesis, and linoleic acid metabolism associated with both jasmonic acid and flavonoid biosynthesis. Ultimately, we proposed a preliminary regulatory mechanism of alfalfa cultivar resistance response to aphids feeding based on transcriptome analyses and published documents.

关键词： alfalfa cultivar resistance aphids transcriptomic molecular mechanism

Effects of thermal treatment on physical and mechanical characteristics of coal rock

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Journal of Central South University 2016年第9期23卷 2336-2345页

作者： YIN tu-bing WANG Pin LI Xi-bing SHU Rong-hua YE Zhou-yuan School of Resources and Safety Engineering Central South University Changsha 410083 China School of Energy and Safety Engineering Hunan University of Science and Technology Xiangtan 411201 China

To study the physical and mechanical properties of coal rock after treatment at different temperatures under impact loading, dynamic compression experiments were conducted by using a split Hopkinson pressure bar(SHPB). The stress–strain curves of specimens under impact loading were obtained, and then four indexes affected by temperature were analyzed in the experiment: the longitudinal wave velocity, elastic modulus, peak stress and peak strain. Among these indexes, the elastic modulus was utilized to express the specimens' damage characteristics. The results show that the stress–strain curves under impact loading lack the stage of micro-fissure closure and the slope of the elastic deformation stage is higher than that under static loading. Due to the dynamic loading effect, the peak stress increases while peak strain decreases. The dynamic mechanical properties of coal rock show obvious temperature effects. The longitudinal wave velocity, elastic modulus and peak stress all decrease to different extents with increasing temperature, while the peak strain increases continuously. During the whole heating process, the thermal damage value continues to increase linearly, which indicates that the internal structure of coal rock is gradually damaged by high temperature.

关键词： rock mechanical property split Hopkinson pressure bar (SHPB) high temperature coal rock dynamic mechanical property

Including predator presence in a refined model for assessing resistance of alfalfa cultivar to aphids

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Journal of Integrative Agriculture 2018年第2期17卷 397-405页

作者： tu Xiong-bing FAN Yao-li Mark McNeill ZHANG Ze-hua State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences AgResearch

The aphid quantity ratio（AQR） is defined as the number of aphids on each cultivar divided by the number of aphids on all cultivars. AQR is based on the correlation between aphid populations and their host plants and is an important tool that has been utilized in evaluating Medicago sativa（alfalfa） cultivar resistance to aphids. However, assessment of alfalfa resistance to aphids can be confused by the presence of aphid predators, causing the assessment of plant resistance to aphids to be based on incorrect aphid population data. To refine the AQR and account for the effect of predators on aphid population assessments, we introduced a parameter ‘α＇, corresponding to the predator quantity ratio, and used αAQR as the ratio to quantify aphid populations. Populations of both aphids（4 species） and their predators（12 species） occurring in 28 M. sativa cultivars were sampled over two years at a research station near Cangzhou, Hebei Province, China. Results showed that the most suitable evaluation period was from May to June, as the aphid population was stable during this period. Compared with the AQR method, the predator population numbers based on the αAQR had a significant inverse relationship with aphid population numbers and the 28 cultivars were clustered into three classes： the resistant class, tolerant class, and susceptible class. In addition, 17 cultivars were reassigned when evaluated using αAQR. All numerical values calculated by αAQR were displayed as a Gaussian distribution, which showed that the 28 cultivars could be clustered into nine groups using a median value（±SE） of 1±0.1. Hence, ongoing alfalfa breeding trials will be assessed using the αAQR to establish a robust system that includes agronomic performance parameters in order to generalize the new method for further studies.

关键词： aphid quantity ratio(AQR) model alfalfa cultivar resistance to aphids natural enemy

Preparation of Spherical Microfine Silicon Powder

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Journal of Wuhan University of Technology(Materials Science) 2008年第4期23卷 440-442页

作者： SHENG Yong ZOU Jun LI bing tu Mingjing College of Material Science and Engineering Sichuan University Chengdu 610065 China

Under certain conditions of proper temperature and pH value, sodium silicate was hydrolyzed in liquid ammonia chloride, and spherical microfine SiO2 powder in micrometer-size was prepared. In this experiment, the relationship between needed time and proper pH value, temperature, density of the solution, and its current capacity were found .The optimum conditions are pH 8.5, 70 ~C, and the concentration of sodium silicate is 0.6 mol/L for the density of the solution. The structure of spherical microfine silicon was characterized by SEM and XRD.

关键词： SiO2 powder spherical-structure

Structural form selection of the high-rise buildingwith the improved BP neural network

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High Technology Letters 2020年第1期26卷 92-97页

作者： Zhao Guangzhe Yang Hanting tu bing Zhou Meiling Zhou Chengle School of Electrical and Information Engineering Beijing University of Civil Engineeringand ArchitectureBeijing 100044P.R.China College of Information and Communication Engineering Hunan Institute of Science and TechnologyYueyang 414000P.R.China

As civil engineering technology development,the structural form selection is more and more critical in design of high-rise ***,structural form selection involves expertise knowledge and changes with the environment which makes the task *** approach utilizing improved back propagation(BP)neural network optimized by the Levenberg-Marquardt(L-M)algorithm is proposed to extract the main controlling factors of structural form ***,an intelligent expert system with artificial neural network is constructed to design high-rise buildings structure *** experiment tests the model in 15 well-known architecture samples and get the prediction accuracy of 93.33%.The results show that the method is feasible and can help designers select the appropriate structural form.

关键词： back propagation(BP)neural network high-rise building structural form selection Levenberg-Marquardt(L-M)algorithm

Laparoscopic liver resection for hepatocellular carcinoma complicated with significant portal hypertension:A propensity score-matched survival analysis

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Hepatobiliary & Pancreatic Diseases International 2023年第4期22卷 358-365页

作者： Zhang-You Guo Yuan Hong bing tu Yao Cheng Xiao-Mei Wang Department of Minimally Invasive Interventional Therapy Yunnan Cancer HospitalThe Third Affiliated Hospital of Kunming Medical UniversityYunnan Cancer CenterKunming 650118China Medical Laboratory The First Affiliated Hospital of Kunming Medical UniversityInstitute of Experimental Diagnostics of Yunnan ProvinceKey Laboratory of Laboratory Medicine of Yunnan ProvinceKunming 650032China Department of Hepatobiliary Surgery The Second Affiliated HospitalChongqing Medical UniversityChongqing 400010China

Background: Significant portal hypertension(SPH) is a relative contraindication for patients with resectable hepatocellular carcinoma(HCC). However, increasing evidence indicates that liver resection is feasible for HCC patients with SPH. Methods: HCC patients with cirrhosis who underwent laparoscopic liver resection(LLR) in two centers from January 2013 to April 2018 were included. Surgical and survival outcomes were analyzed to explore potential prognostic factors. Propensity score matching(PSM) analysis was performed to minimize bias. Results: A total of 165 patients were divided into two groups based on the presence(SPH, n = 76) or absence(non-SPH, n = 89) of SPH. Patients in the SPH group had longer operative time, more blood loss, and more advanced TNM stage than patients in the non-SPH group( P 0.05). After PSM, alpha-fetoprotein(AFP) ≥ 400 μg/L [hazard ratio(HR) = 4.71, 95% confidence interval(CI): 2.69-8.25], ascites(HR = 2.18, 95% CI: 1.30-3.66), American Society of Anesthesiologists(ASA) classification(Ⅲ vs. Ⅱ)(HR = 2.13, 95% CI: 1.11-4.07) and tumor diameter > 5 cm(HR = 3.91, 95% CI: 2.02-7.56) independently predicted worse OS. Conclusions: LLR for patients with HCC complicated with SPH appears feasible at the price of increasing operative time and blood loss. AFP, ascites, ASA classification and tumor diameter may predict the prognosis of HCC complicat

关键词： Hepatocellular carcinoma Significant portal hypertension Laparoscopic liver resection Propensity score matching analysis

Spectral‐spatial sequence characteristics‐based convolutional transformer for hyperspectral change detection

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CAAI Transactions on Intelligence Technology 2023年第4期8卷 1237-1257页

作者： Chengle Zhou Qian Shi Da He bing tu Haoyang Li Antonio Plaza School of Geography and Planning Sun Yat‐sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory for Urbanization and GeoSimulation Sun Yat‐sen UniversityGuangzhouChina Institute of Optics and Electronics Nanjing University of Information Science and TechnologyNanjingChina Hyperspectral Computing Laboratory Escuela PolitecnicaUniversity of ExtremaduraCaceresSpain

Recently,ground coverings change detection(CD)driven by bitemporal hyperspectral images(HSIs)has become a hot topic in the remote sensing *** are two challenges in the HSI‐CD task:(1)attribute feature representation of pixel pairs and(2)feature extraction of attribute patterns of pixel *** solve the above problems,a novel spectral‐spatial sequence characteristics‐based convolutional transformer(S3C‐CT)method is proposed for the HSI‐CD *** the designed method,firstly,an eigenvalue extrema‐based band selection strategy is introduced to pick up spectral information with salient attribute ***,a 3D tensor with spectral‐spatial sequence characteristics is proposed to represent the attribute features of pixel pairs in the bitemporal ***,a fusion framework of the convolutional neural network(CNN)and Transformer encoder(TE)is designed to extract high‐order sequence semantic features,taking into account both local context information and global sequence ***,a spatial‐spectral attention mechanism is employed to prevent information reduction and enhance dimensional interactivity between the CNN and ***,the binary change map is determined according to the fully‐connected *** results on real HSI datasets indicated that the proposed S3C‐CT method outperforms other well‐known and state‐of‐the‐art detection approaches in terms of detection performance.

关键词： change detection imageanalysis

IPM-Biological and integrated management of desert locust

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Journal of Integrative Agriculture 2022年第12期21卷 3467-3487页

作者： LI Shuang FENG Shi-qian Hidayat ULLAH tu Xiong-bing ZHANG Ze-hua State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China Chongqing Academy of Agricultural Sciences Chongqing 401329P.R.China Department of Agriculture The University of SwabiAnbar-23561Khyber PakhtunkhwaPakistan Scientific Observation and Experimental Station of Pests in XilinGol Rangeland Institute of Plant ProtectionChinese Academy of Agricultural SciencesXilinhot 026000P.R.China

Locusts have caused periodic disasters in the recorded history of *** to now,locust disaster is still the biggest threat to the world’s agricultural *** desert locust Schistocerca gregaria is one of the most harmful locusts,which has caused massive food crises,economic losses,and ecological *** desert locust is a migratory insect pest that occurs year-round in the tropic and subtropical *** the wind and seasonal alternation,it moves and flies in the African continent and West *** locust damages the stems and leaves of more than 300 plants,including Gramineae,Tribulus terrestris,and *** cause devastating disasters to local plants,especially field crops,and significantly threaten food *** date,voluminous research has been conducted regarding the ecology and management of desert *** review represents an effort to summarize the basic information on the biology and ecology,distribution,damage,and economic impact of desert locusts,examine the recent developments in integrated locust management,and make recommendations for future research.

关键词： Schistocerca gregaria crops Orthoptera locust agriculture production ecology

Devonian Bivalve Faunas, Life-habits, and Biodiversity Changes in Guangxi, South China

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Acta Geologica Sinica(English Edition) 2016年第1期90卷 378-379页

作者： YAO Huazhou ZHANG Renjie tu bing YAN Daoping TANG Zhaoyang Wuhan Institute of Geology and Mineral Resources China Geological Survey

Objective The Devonian bivalves are well developed, highly diversified and widely distributed in Guangxi. However, they have not been well studied previously, although several papers have dealt with it. Meanwhile, the biodiversity change of bivalves which is one of the most important benthos during Devonian in South China has not been studied yet. The aim of present project is focused on the study of systematic palaeontology, the life-habits, of Devonian bivalves of Guangxi.

关键词： Devonian Bivalve Faunas Life-habits and Biodiversity Changes in Guangxi South China