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Effect of protein molecules on the photoluminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots

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Chinese Science Bulletin 2013年第21期58卷 2616-2621页

作者： ZHANG YouLin tu langping ZENG QingHui KONG XiangGui State Key Laboratory of Luminescence and Applications Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences

The fact that the photoluminescence properties of quantum dots are always strongly influenced by the environment limits the scope of further progress in the field of QD' bio-applications. In this paper, the effects of immunoglobulin G (IgG) on the photo-luminescence properties and stability of water-soluble CdSe/ZnS core-shell quantum dots coated with amphiphilic poly (acrylic acid) (PAA) are studied. Photoluminescence (PL) spectra, UV-vis spectra and excited state lifetime measurements are used to characterize the influence of different protein molecules, such as IgG (goat anti-human IgG, rabbit anti-human IgG, human IgG, and goat anti-human IgG-human IgG conjugates), avidin and bovine serum albumin (BSA) on the PL properties of QDs. The PL intensity and stability of CdSe/ZnS are largely enhanced compared to that of pure CdSe/ZnS QDs when the IgG molecules are added into the QD solution. The PL intensity increases with increasing the IgG concentration, but there appears no influence on the PL peak and a full width at half maximum (FWHM). The PL evolution of QDs as a function of different protein molecules depends on the structure of protein molecules, which is used as a sensor to recognize human IgG. It is inferred that the interaction between PAA coating layer and IgG molecules results in the enhancement of PL intensity. The study of the effect of pH and ion strength on optical properties of QD-IgG mixed solution, compared with the pure QD solution, suggests that pH value and ion strength do not destroy the interaction between the PAA coating layer and IgG. Excited state lifetime analysis indicates that the PL enhancement comes from the passivation of surface of the QDs with the PAA coating layer. IgG molecules have no effects on the properties of the biological system but can increase the stability and PL intensity of CdSe/ZnS QDs, which will enlarge the application of QDs in biomedicine and other fields.

关键词：光致发光特性蛋白质分子 CdSe 量子点稳定性 ZnS 水溶性抗生物素蛋白

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Internal OH^(-)induced cascade quenching of upconversio n luminesce nee in NaYF4:Yb/Er nanocrystals

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Light(Science & Applications) 2021年第6期10卷 1078-1087页

作者： Yansong Feng Zhi Li Qiqing Li Jun Yuan langping tu Lixin Ning Hong Zhang van't Hoff Institute for Molecular Sciences University of AmsterdamScience Park 9041098XH AmsterdamThe Netherlands. State Key Laboratory of Explosion Science and Technology School of Mechatronical EngineeringBeijing Institute of TechnologyBeijing 100081China. Anhui Key Laboratory of Optoelectronic Materials Science and Technology Department of PhysicsAnhui Normal University241000 WuhuChina. State Key Laboratory of Luminescenee and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033 JilinChina

Internal hydroxyl impurity is known as one of the main detrimental factors affecting the upconversion(UC)efficiency of upconversion luminescence(UCL)*** from surface"ligand-related emission quenching which can be effectively diminished by,e.g.,core"shell structure,internal hydroxyl is easy to be introduced in synthesis but difficult to be quantified and ***,it becomes an obstacle to fully understand the relevant UC mechanism and improve UC efficiency of *** we report a progress in quantifying and large-range adjustment of the internal hydroxyl impurity in NaYF4 *** combining the spectroscopy study and model simulation,we have quantitatively unraveled the microscopic in teractions underlying UCL que nching between internal hydroxyl and the sensitizers and activators,***,the internal hydroxyl-involved UC dynamical process is interpreted with a vivid concept of"Survivor effect" i.e.;the shorter the migration path of an excited state,the larger the possibility of its surviving from hydroxyl-induced quenchi *** from the consist ent experimental results,this concept can be further evidenced by Monte Carlo simulation,which monitors the variation of energy migration step distribution before and after the hydroxyl *** new quantitative insights shall promote the construction of highly efficient UC materials.

关键词： materials. quenching excited

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Preparation of Silver Nano-triangular Prisms and Their SERS of IgG Protein

Preparation of Silver Nano-triangular Prisms and Their SERS ...

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第八届全国光生物学学术会议

作者： Bing Xue langping tu Xianggui Kong State Key Laboratory of Fluorescence and Applications Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

Ag nano-triangular prisms have has drawn a great deal of interests of investigators because of their unique optical properties which make the surface-enhanced Raman scattering（SERS） enhancing, which make some large advantages in analysis of molecules, especially biomolecules. In the present study, Ag nano-triangular prisms were synthesized through synthetic route of single-beam excitation by simply adjusting solution pH. Shape and size effects of silver nano-triangular prisms on SERS properties were studied, and found that different-shaped and size Ag triangular prisms take on different SERS. Specially, the Ag nano-triangular prisms with a equilateral could enhance SERS sensitivity over 90-fold as compared with those from silver nano-spheres with about 40 nm in size. The highly strong SERS of silver triangular prisms were demonstrated from anti-IgG molecules in solution. The Ag nano-triangular prisms with high sensitivity of SERS will be combined optical fibre biosensor in our laboratory for structuring a novel optical fibre biosensor without biolabeling to detect some targeting molecules in vivo, bioassay, biodiagnosis, and even clinical diagnosis.

关键词： Ag triangular prisms SERS without biolabeling optical fibre biosensor

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Upconverting Fluorescence Nanoconjugations Used in Biomaging/Biotracing and Kill of Cancer Cells

Upconverting Fluorescence Nanoconjugations Used in Biomaging...

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第八届全国光生物学学术会议

作者： Xianggui Kong langping tu Lu Xia Xiaomin Liu State Key Laboratory of Fluorescence and Applications Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

Under light excitation,the Photosenstizers(PS) can interact with molecular oxygen and generate cytotoxic singlet oxygen(1O2);And the inherent fluorescence from PS can also be used for fluorescence imaging/biotrancing,often called as photodynamic diagnosis(PDD). However, phototriggered theranostics based on traditional PS molecules suffer mainly from low signal-to-noise ratio, small tissue penetration depth, and photobleaching. In addition, two different wavelength lights are always adopted to implement respectively imaging and therapy, which makes it difficult for PDD and PDT. The contradictory requirements of high 1O2 production for PDT and strong fluorescence for PDD make it difficult to realize simultaneous PDD and PDT. To meet the demands of high fluorescent intensity and high 1O2 production yield, a highly efficient multifunctional nanoconjugations for simultaneous upconversion fluorescence imaging and photodynamic therapy has been developed on the basis of selective energy transfer from multicolor luminescent NaYF4:Yb3+, Er3+ upconversion nanoparticles(UCNP) to PS. Different from popular approaches based on electrostatic or hydrophobic interactions, over 100 PS molecules were covalently bonded to every 20 nm UCNP, which significantly strengthened the UCNP-PS linkage and reduced the probability of leakage/desorption of the PS. Over 80% UCL was transferred to PS, and the singlet oxygen production was readily detected by its feature emission at 1270 nm. Tests performed on JAR choriocarcinoma and NIH 3T3 fibroblast cells verified the efficient endocytosis and PDT of the nanoconjugations with 980 nm irradiation specific to JAR cancer cells. Our work highlights the promise of using UCNPs for potential image-guided cancer PDT.

关键词： fluorescnece upconversion nanoconjugations covalent bonding fluorescence resonant energy transfer singlet oxygen fluorescence imaging photodynamic therapy.

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Studies on the Preparation of Aptamer Optical Bio-switch Based on the Nanocomplexes of Upconversion Luminescence Nanoparticle and Quantum Dot

Studies on the Preparation of Aptamer Optical Bio-switch Bas...

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第八届全国光生物学学术会议

作者： langping tu Yi Yu Lu Xia Fei Wu Xianggui Kong State Key Laboratory of Fluorescence and Applications Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences University of Chinese Academy of Sciences

Aptamer is superior to traditional antibodies, in terms of their small size, long storage, high reproducibility and easy modification as recognition elements in biosensors. Aptamers can be folded into diversely defined tertiary structures, resulting in a shape-complementary recognition between aptamers and targets via hydrogen bonds, van der Waals interactions or base-stacking interactions. The significant conformational change upon target binding offers remarkable flexibility and convenience in designing novel biosensors. In particular, it is worth mentioning that photoactivation of QDs is not favourable for stability of conjugated nucleic acids. To solve the problem of photo-induced ROI on the breakage and damage of aptamers, we propose and validate a novel and simple optical bio-switch based on aptamer-upconversion nanoparticles（UCNPs） bioconjugates. Although there are several reports on the use of UCNPs as QDs substitutes due to the unique characteristics of these new class of photoluminescent labels, there have been little studies on the breakage and damage of photoactivated UCNPs upon biological samples. In the present paper, it was showed for the first time that, in contrast to photoactivated QDs, the UCNPs in themselves do not directly generate ROI using near-infrared（NIR） illumination, illustrating that photosensitized breakage and damage upon aptamers are eliminated by employing UCNPs as donors. LRET-based optical bio-switch of an aptamer- UCNPs conjugate was constructed. It is demonstrated that photosensitized breakage and damage of aptamers are eliminated by employing UCNPs as donors, and the as-designed biosensor is specific and sensitive in the detection of ATP.

关键词： aptamer optical bio-switch upconversion nanoparticles photo breakage and damage of adapter molecules detection of ATP

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反向能量传递增强Er^(3+)上转换发光

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发光学报 2023年第9期44卷 1552-1559页

作者：杨润陈浩然涂浪平李齐清常钰磊中国科学院长春光学精密机械与物理研究所发光学及应用国家重点实验室吉林长春130033 中国科学院大学北京100049

提高上转换发光效率是促进上转换发光材料实际应用的关键。在NaErF_(4)@NaYF_(4)体系中,惰性NaYF_(4)壳层可以抑制高组分Er^(3+)掺杂下的发光浓度猝灭,其上转换发光主要来源于Er^(3+)-Er^(3+)的能量传递上转换。本文利用共沉淀法制备了E... 详细信息

提高上转换发光效率是促进上转换发光材料实际应用的关键。在NaErF_(4)@NaYF_(4)体系中,惰性NaYF_(4)壳层可以抑制高组分Er^(3+)掺杂下的发光浓度猝灭,其上转换发光主要来源于Er^(3+)-Er^(3+)的能量传递上转换。本文利用共沉淀法制备了Er^(3+)和Yb^(3+)分区掺杂的NaErF_(4)@NaYbF_(4)@NaYF_(4)核壳结构的纳米颗粒,通过包覆惰性壳层研究Er^(3+)-Yb^(3+)-Er^(3+)之间的能量传递和反向能量传递过程。由于808 nm波长只能激发Er^(3+)而不能激发Yb^(3+),因此在808 nm波长激发下,Er^(3+)在惰性壳层的保护作用下将激发态能量传递给Yb^(3+),随后通过反向能量传递回Er^(3+),使得Er^(3+)的上转换发光增强。实验结果发现,当中间层Yb^(3+)掺杂浓度为100%时,绿色和红色上转换发光最大增强倍数为24.9和9.79。

关键词：稀土纳米材料上转换发光反向能量传递 β-NaErF_(4)

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Effects of Coupled Biomolecules on Luminescent Properties of Type-ⅡCdTe/CdS Core/Shell Quantum Dots in Aqueous Solution

Effects of Coupled Biomolecules on Luminescent Properties of...

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第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Qinghui Zeng~1,Youlin Zhang~1,Xiaomin Liu~1,langping tu~1,Yajuan Sun~1,Xianggui Kong~1 1.Key Laboratory of Excited State Processes,Changchun Institute of Optics,Fine Mechanics and Physics, Chinese Academy of Sciences,3888 Eastern South Lake Road,Changchun 130033,China

3-mercaptopropionic acid-stabilized type-Ⅱ CdTe/CdS core/shell quantum dots(QDs) were prepared in an aqueous solution following the synthetic route of successive ion layer adsorption and *** different properties of e... 详细信息

关键词： CdTe/CdS type-Ⅱquantum dots biocoupling immunoassay specificity and sensitivity

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Er^(3+)/Tm^(3+)分区掺杂结构的比率型光子上转换低温探针

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发光学报 2023年第8期44卷 1335-1343页

作者：吴晗陈浩然解小雨涂浪平李齐清孔祥贵常钰磊中国科学院长春光学精密机械与物理研究所发光学及应用国家重点实验室吉林长春130033 中国科学院大学北京100049

利用溶剂热法制备了六角相NaYF_(4)∶20%Yb,2%Er@NaYF_(4)@NaYbF_(4)∶0.5%Tm@NaYF_(4)多层核壳结构稀土掺杂上转换纳米粒子,研究其在低温场(10~295 K)及980 nm激发下分别来自于Er^(3+)的绿色与红色以及Tm^(3+)的蓝色发光的上转换发光... 详细信息

利用溶剂热法制备了六角相NaYF_(4)∶20%Yb,2%Er@NaYF_(4)@NaYbF_(4)∶0.5%Tm@NaYF_(4)多层核壳结构稀土掺杂上转换纳米粒子,研究其在低温场(10~295 K)及980 nm激发下分别来自于Er^(3+)的绿色与红色以及Tm^(3+)的蓝色发光的上转换发光性质。结果显示,绿光强度随温度升高呈现出先增后降的变化趋势,而蓝光强度随温度升高呈现热衰减的趋势。本工作利用发光强度比的测温方法实现了精准的温度测量,相对灵敏度可达3.2%·K^(-1)。并通过改变外层发光壳层的厚度调节发光强度比,进一步应用于低温场光学防伪。

关键词：稀土纳米粒子发光强度比测温上转换发光低温场防伪

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A Multi-Sensor Fusion Algorithm for Pedestrian Navigation Using Factor Graphs

A Multi-Sensor Fusion Algorithm for Pedestrian Navigation Us...

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第40届中国控制会议

作者： langping An Xianfei Pan Ze Chen Mang Wang Zheming tu Chaoqun Chu College of Intelligence Science and Technology National University of Defense Technology

Real-time and robust navigation for pedestrians is a challenging problem in the satellite denial environment. When designing an autonomous navigation framework for pedestrians, one often estimates local pose with either the visual odometer or foot-mounted MIMU. However, the framework has poor robustness in diverse environments owing to the shortcomings of vision and inertia. In this paper, we propose a pedestrian navigation algorithm to estimate local pose by fusing foot-mounted MIMU with cameras and optimize the state with other sensors. Different from sensors rigid connection, the local sensors are attached to the pedestrian, which is a flexible body but has a similar position estimation in diverse parts of the body. The global state is optimized in batch with the factor graph. Therefore, the accumulated drifts of inertial integral are corrected and the robustness of system is improved. We evaluate the performance of our algorithm with real-world experiments. Results are compared against other algorithms to show that the absolute trajectory accuracy in the algorithm has been greatly improved.

关键词： Pedestrian Navigation Sensors Fusion Factor Graph

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