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Chemical Research in Chinese Universities 2010年第2期26卷 268-271页

作者： LIU Xiao-ping WANG Ying LAN Hui-yu SONG Ai-hua tsim karl wah-keung DONG Tina Ting-xia HU Chun Department of Organic Chemistry School of Pharmaceutical Engineering Shenyang Pharmaceutical University Shenyang 110016 P. R. China Tianjin Key Laboratory of Chemistry and Analysis of Chinese Materia Medica Tianjin University of Traditional Chinese Medicine Tianjin 300193 P. R. China Department of Biology The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong P. R. China

Flavonoids exhibit a wide range of biological activities including anti-inflammatory activity, while some 1,3-benzoxazine derivatives show anti-inflammatory activity. In order to explore the novel anti-inflammatory agents, based on the principles of bioisosterism and hybridization, ten novel ehromeno[8,7-e][1,3]oxazin-4-ones were syn- thesized and characterized with IR, ^1H NMR, MS and elemental analyses. The anti-inflammatory activities of the target compounds were evaluated via the croton oil-induced ear oedema test in Swiss mice. According to screened resuits, some target compounds show potent anti-inflammatory activity.

关键词： Anti-inflammatory activity Heterocycle Flavone Chromeno[8,7-e]oxazin-4-one

Luteolin， a common flavone in vegetables， acts as a hormetin possessing neurotrophic function: signaling mediated by inducing mitochondrial stress

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Food Science and Human Wellness 2024年

作者： Alex Xiong Gao Tracy Chenxi Xia Maggie Suisui Guo Gary Kawing Yuen Kevin Qiyun Wu Tina Tingxia Dong karl wah-keung tsim Shenzhen Key Laboratory of Edible and Medicinal Bioresources， HKUST Shenzhen Research Institute Division of Life Science and Center for Chinese Medicine， The Hong Kong University of Science and Technology

This study aims to uncover the hormetic process of luteolin， a common dietary flavone， in neuronal cells through its role in inducing mitochondrial stress. In rat pheochromocytoma PC12 cells， luteolin at low concentrations caused a mild and reversible loss of mitochondrial membrane potential (MMP)， while high concentrations of luteolin triggered intense and sustained depolarization of MMP. The MMP disturbance was shown to have a close association with the trophic and/or toxic effects triggered by luteolin; because the common mitochondrial uncouplers shared similar bi-phase dose-response on cell viability， as that of luteolin. Along with the induced MMP pertubation， luteolin triggered the development of autophagy and mitophagy， as determined by mCherry-GFP-LC3B tandem protein， and by the co-localization of mitochondrial/lysosomal staining. Subsequent application of autophagy inhibitors in the cultures blocked the luteolin-induced neurotrophic activities and sensitized the cells to be less resistant to luteolin-mediated cytotoxicity. Other flavonoids also displayed similar properties in the cultures， indicating that these compounds act as hormetic pharmacological inducers that stimulate cells to become more resilient and adapt to threats. This study provides a unifying mechanistic explanation for the neuro-beneficial effects of luteolin and other flavonoids.

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甘肃贝母的组织培养和植株再生研究

北方园艺 2014年第10期 80-84页

作者：张振霞李彩萍张秋枚董婷霞詹华强郑玉忠韩山师范学院生物学系广东潮州521041 香港科技大学生命科学部香港999077

以甘肃贝母地下鳞茎为试材,研究了不同消毒方法、不同激素组合对其鳞茎的诱导增殖及植株再生的影响,以期建立甘肃贝母的快速无性繁殖体系。结果表明:用70%乙醇处理15s,0.1%升汞处理10min,无菌水洗5次,然后在4℃中冷藏过夜,第2天重复上... 详细信息

以甘肃贝母地下鳞茎为试材,研究了不同消毒方法、不同激素组合对其鳞茎的诱导增殖及植株再生的影响,以期建立甘肃贝母的快速无性繁殖体系。结果表明:用70%乙醇处理15s,0.1%升汞处理10min,无菌水洗5次,然后在4℃中冷藏过夜,第2天重复上述灭菌步骤的连续消毒的方式灭菌效果很好,其染菌率为16.7%;诱导鳞茎不定芽的最佳培养基是MS+NAA2.0mg/L+6-BA 0.5mg/L,出芽率较高,为87.5%,能分化出的绿色健壮的不定芽;继代增殖培养基是MS+NAA 0.5mg/L+6-BA 2.0mg/L,增殖系数达到9;生根的最佳培养基是1/2MS+NAA 0.5mg/L,生根率90%。该试验基本建立了甘肃贝母植株的再生体系,为其愈伤组织的增殖和完整植株的再生提供了重要的研究基础。

关键词：甘肃贝母组织培养鳞茎增殖

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参麦注射液中麦冬化学成分的鉴定

中成药 2017年第11期39卷 2340-2344页

作者：李宁黄星王冰张加余王怀友董婷霞詹华强屠鹏飞香港科技大学深圳研究院广东深圳518057 上海中医药大学教学实验中心上海201203 北京中医药大学科研实验中心北京100029 北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

目的鉴定参麦注射液中麦冬Ophiopogon japonicus(L.f.)Ker-Gawl.的化学成分。方法采用HPLC-ESI-MSn法进行鉴定,条件为Agilent SB-C18色谱柱(250 mm×4.6 mm,5μm);流动相A为乙腈-甲醇(3∶1),流动相B为0.1%甲酸,梯度洗脱;体积流量1.0 m L/min;柱温30℃。结果从中鉴定出15种麦冬化学成分。结论该方法快速灵敏,可为参麦注射液质量标准的提高提供依据。

关键词：参麦注射液麦冬化学成分 HPLC-ESI-MSn

Characterization of edible bird’s nest by peptide fingerprinting with principal component analysis

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Food Quality and Safety 2017年第1期1卷 83-92页

作者： Chun-Fai Wong Gallant Kar-Lun Chan Ming-Lu Zhang Ping Yao Huang-Quan Lin Tina Ting-Xia Dong Geng Li Xiao-Ping Lai karl wah-keung tsim Division of Life Science and Center for Chinese Medicine R&D The Hong Kong University of Science and TechnologyClear Water BayKowloon HKUST Shenzhen Research Institute Hi-Tech ParkNanshan School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine Dongguan Mathematical Engineering Academy of Chinese Medicine Guangzhou University of Chinese MedicineChina

OBJECTIVES:Proteins are the major component and play a key role in nutritious and therapeutic functions of edible bird’s nest(EBN);however,limited studies have been conducted on the protein due to difficulties in extraction,isolation as well as *** study aimed to provide comprehensive information for the quality evaluation of EBN peptides,which would be a valuable reference for further study on EBN ***:Here,we developed a quality control method using high performance liquid chromatography(HPLC)peptide fingerprints deriving from EBN being digested with simulated gastric *** characteristic peptide peaks were collected and identified by LC-MS/***:The characteristic peptide peaks,corresponding to the protein fragments of acidic mammalian chitinase-like,lysyl oxidase,and Mucin-5AC-like,were identified and ***,the principal component analysis indicated that the fingerprints were able to discriminate colour of EBN(white/red)and production sites(cave/house)of White EBN on the same weight *** proposed by the model developed in this study,Muc-5AC-like and AMCaselike proteins were the markers with the highest discriminative ***:The overall findings suggest that HPLC peptide fingerprints were able to clearly demonstrate peptide profile differences between genuine and adulterated EBN samples;and classify EBN samples by its color and production *** addition,the protein identification results suggested that Muc-5AC-like protein was the major protein in EBN.

关键词： Edible bird’s nest Peptide fingerprint Mucin-5AC-like Acidic mammalian chitinase-like Principal component analysis

An optimization of ultra-sonication-assisted extraction from flowers of Apocynum venetum in targeting to amount of free amino acids determined by UPLC-MS/MS

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Food Quality and Safety 2019年第1期3卷 52-60页

作者： Yan Jin Caroline Yang Wang Weihui Hu Yun Huang Miranda Li Xu Huaiyou Wang Xiangpeng Kong Yicun Chen Tina Tingxia Dong Qiwei Qin karl wah keung tsim Shenzhen Key Laboratory of Edible and Medicinal Bioresources HKUST Shenzhen Research InstituteHi-Tech ParkNanshanShenzhen518000China Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and TechnologyClear Water BayHong KongChina Pharmacology Department Shantou University Medical CollegeShantou515041China Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation College of Marine SciencesSouth China Agricultural UniversityGuangzhou 510642PR China

Amino acids are naturally occurring compounds in many edible or medicinal plants,which possess a variety of nutraceutical effects in ***,a method of ultrasound-assisted extraction was optimized using Box-Behnken design of response surface methodology in maximizing the yield of free amino acids deriving from flowers of Apocynum venetum *** the optimal condition of ultrasound-assisted extraction,i.e.187.5 W of ultrasonic power,31.93 min of extraction time,and 0.47 mg/ml of solid-liquid ratio,the experimental yield of extractive was 287.17±0.205 mg/g,which was in close agreement with the value,as predicted by the established *** addition,a hydrophilic interaction ultra-high performance liquid chromatography coupled with QqQ-MS/MS method was developed and validated for simultaneous quantification of 20 types of amino acids without derivatization contained in *** *** analytical method was validated by matrix effect,linearity,limit of detection,limit of quantification,precision,repeatability,stability,and *** analysis results showed that *** flower was rich in free amino acids of~3%of total dried weight,and which contained leucine(13.7 g/mg),isoleucine(7.9 g/mg),and lysine(2.2 g/mg)as the most abundant amino ***,*** flower could provide beneficial nutrient values for human health,*** weight maintenance or glucose homeostasis.

关键词： Apocynum venetum response surface methodology ultra-sonication-assisted extraction amino acid UPLC-MS/MS

DALP Analysis on Genetic Diversity of <i>Panax notoginseng</i>

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Chinese Medicine 2014年第2期5卷 123-129页

作者： Xiuming Cui Hui Xiao Jingjing Yang Tina Dong karl wah-keung tsim Yuqin Sun Yan Zhu College of Life Science and Technology Kunming University of Science and Technology Kunming China Department of Biology and Biotechnology Research Institute The Hong Kong University of Science and Technology Hong Kong China Wenshan Sanqi Research Institute Wenshan College Wenshan China

Panax notoginseng (Burk) F.H. Chen is one of the most famous Chinese traditional medicinal plants, which belongs to Panax genus under Araliaceae family. At the present, Panax notoginseng cultivated is a mix colony. Using DALP makers, this article studied on genetic diversity of cultivated populations of Panax notoginseng in Wenshan County of Yunnan province and Napo country of Guangxi province. And the results showed that there were 260 polymorphic loci detected from the total of 292 in 13 populations, and there was great part of genetic diversity found between populations and the genetic differentiations were lower within populations. So there is broad prospect in good species breeding. And it can provide basic information for resource protection and sustainable use of Panax notoginseng.

关键词： Panax notoginseng Genetic Diversity DALP

An UPLC-MS/MS method for simultaneous determination of multiple constituents in Guizhi Fuling capsule with ultrafast positive/negative ionization switching

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Chinese Journal of Natural Medicines 2018年第4期16卷 313-320页

作者： ZHANG Rong-Hua LI Chao-Ran YANG Hua LI Meng-Ning karl W.K.tsim LI Ping GAO Wen State Key Laboratory of Natural Medicines School of Traditional Chinese Pharmacy China Pharmaceutical University School of Traditional Chinese Medicine Guangdong Pharmaceutical University Division of Life Science and Center for Chinese Medicine The Hong Kong University of Science and Technology

Guizhi Fuling capsule(GFC), a traditional Chinese medicine(TCM) with effects of promoting blood circulation and dissipating blood stasis, has been widely used in the clinic. Because of the complex matrix and various chemical structure types, quality control of GFC remains great challenge. In the present study, an ultra performance liquid chromatography hybrid triple-quadrupole mass spectrometry(UPLC-QQQ MS) method with ultrafast positive/negative ionization switching was developed for simultaneous determination of 18 bioactive components in GFC, including methyl gallate, ethyl gallate, oxypaeoniflorin, benzoic acid, albiflorin, paeonolide, paeoniflorin, 1, 2, 3, 4, 6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, pachymic acid, amygdalin, cinnamaldehyde, paeonol, cinnamic acid, 4-hydroxybenzoic acid, and gallic acid. Separation was performed on an Agilent Zorbax Extend–C18 column(2.1 mm × 50 mm, 1.8 μm), using a gradient elution with acetonitrile and water containing 0.1% formic acid. Cholic acid was selected as the internal standard. This newly developed method was fully validated for linearity, precision, accuracy, and stability, and then applied to quality assessment of GFC. Finally, the batch-to-batch reproducibility of GFC samples was evaluated by the cosine ration and Euclidean distance method, which showed high quality consistency. The results demonstrated that the developed method provided a reasonable and powerful manner for quality control of GFC.

关键词： Guizhi Fuling capsule(GFC) UPLC-QQQ MS Ultrafast positive/negative ionization switching Quality control

Edible Bird’s Nest, an Asian Health Food Supplement, Possesses Skin Lightening Activities: Identification of N-Acetylneuraminic Acid as Active Ingredient

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Journal of Cosmetics, Dermatological Sciences and Applications 2015年第4期5卷 262-274页

作者： Gallant Kar Lun Chan Zack Chun Fai Wong Kelly Yin Ching Lam Lily Kwan Wai Cheng Laura Minglu Zhang Huangquan Lin Tina Tingxia Dong karl wah keung tsim Division of Life Science and Center for Chinese Medicine R&D The Hong Kong University of Science and Technology Kowloon Hong Kong China

Edible bird’s nest (EBN;Yan Wo), or cubilose, is originated from the salivary secretion of Aerodramus fuciphagus. In Asia, EBN is famous for its unproven skin lightening function. Here, we aim to reveal the active ingredients of EBN responsible for skin lightening function. Three major fractions were isolated from EBN water extract by chromatography using LC-MS/MS, bioactivities of these isolated fractions were analyzed by assays of tyrosinase, melanocytes and 3D human skin model, from which, N-acetylneuraminic acid (NANA), the second isolated fraction showed an inhibition effect on tyrosinase activity in a dose-dependent manner. The IC50 of tyrosinase originated from mushroom and human was 16.93 mM and 0.10 mM respectively. Furthermore, only EBN with higher content of NANA (e.g. White and Red EBN), but not EBN with less NANA (e.g. Grass EBN), showed promising skin lightening function. This is the first report to reveal NANA being an active ingredient of EBN on skin lightening function.

关键词： Cubilose N-Acetylneuraminic Acid Chemical Analysis Skin Lightening Human Skin Model

The biodiversity of flavonoids from Chinese medicines-development of health food supplements for health promotion and disease prevention

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The biodiversity of flavonoids from Chinese medicines-develo...