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THE EFFECT OF CORNEAL DRYING ON CENTRAL CORNEAL THICKNESS

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国际眼科杂志 2003年第1期3卷 73-74页

作者： V.Dayanir R.Sakarya A.topaloglu E.kir T.Aktunc S.b.Ozkan Med-ical School Adnan Menderes University TurkeyMed-ical School Adnan Menderes University TurkeyMed-ical School Adnan Menderes University TurkeyMed-ical School Adnan Menderes University TurkeyMed-ical School Adnan Menderes University TurkeyMed-ical School Adnan Menderes University Turkey

Purpose: To evaluate the change in central corneal thickness by ultrasongraphic pachymetry induced by corneal drying and to discuss its possible impact on intraocular pressure (IOP). Materials and Methods: 100 eyes of 100 outpatients who were >40 years old were not prescribed any topical medications had no history of intraocular surgery or eye disease had a visual acu-

关键词：每膜干燥角膜中央厚度 IOP 影响超声测厚计

Contribution of functional kir3DL1 to ankylosing spondylitis

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Cellular & Molecular Immunology 2010年第6期7卷 471-476页

作者： Ivan V Zvyagin Ilgar Z Mamedov Olga V britanova Dmitriy b Staroverov Evgeni L Nasonov Anna G bochkova Anna V Chkalina Alexei A Kotlobay Dmitriy O Korostin Denis V Rebrikov Sergey Lukyanov Yuri b Lebedev Dmitriy M Chudakov Shemiakin-Ovchinnikov Institute of bioorganic Chemistry RASMoscowRussia Institute of Rheumatology RAMSMoscowRussia DNA-Technology JSC MoscowRussia Vavilov Institute of General Genetics MoscowRussia

Increasing evidence points to a role for killer immunoglobulin-like receptors(kirs)in the development of autoimmune *** particular,a positive association of kir3DS1(activating receptor)and a negative association of kir3DL1(inhibitory receptor)alleles with ankylosing spondylitis(AS)have been reported by several ***,none of the studies analyzed these associations in the context of functionality of polymorphic *** better understand how the kir3DL1/3DS1 genes determine susceptibility to AS,we analyzed the frequencies of alleles and genotypes encoding functional(kir3DL1*F)and non-functional(kir3DL1*004)*** genotyped 83 AS patients and 107 human leukocyte antigen(HLA)-b27-positive healthy controls from the Russian Caucasian population using a two-stage sequence-specific primer PCR,which distinguishes kir3DS1,kir3DL1*F and kir3DL1*004 *** the patients carrying two functional kir3DL1 alleles,those alleles were additionally genotyped to identify kir3DL1*005 and kir3DL1*007 alleles,which are functional but are expressed at low ***3DL1 was negatively associated with AS at the expense of kir3DL1*F but not of kir3DL1**** finding indicates that the inhibitory kir3DL1 receptor protects against the development of AS and is not simply a passive counterpart of the segregating kir3DS1 allele encoding the activating ***,analysis of genotype frequencies indicates that the presence of kir3DS1 is a more important factor for AS susceptibility than the absence of kir3DL1**** activation of either natural killer(NK)or T cells via the kir3DS1 receptor can be one of the critical events in AS development,while the presence of the functional kir3DL1 receptor has a protective ***,even individuals with a genotype that carried two inhibitory kir3DL1 alleles expressed at high levels could develop AS.

关键词： ankylosing spondylitis autoimmune disease HLA-b27 kir kir3DL1 kir3DS1 kir3DL1*004 NK cell T cell

Pharmacist-Managed Anticoagulation Services for Warfarin Management in Tertiary Hospitals:The Egyptian Experience

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Journal of Clinical and Nursing Research 2022年第3期6卷 24-30页

作者： Amira b Kassem Asmaa M Farrag Dina Shafea Osama Mohamed Ibrahim Department of Clinical Pharmacy and Pharmacy Practice Faculty of Pharmacy Damanhour university Egypt Abo-kir General Hospital Ministry of HealthAlexandriaEgypt Department of Pharmacy Practice and Pharmacotherapeutics Faculty of PharmacyUniversity of SharjahUAE Department of Clinical Pharmacy Faculty of PharmacyCairo University Egypt

background:Warfarin is widely regarded as the main anticoagulant in lowering the risk of *** study used indicators to compare pharmacist-managed anticoagulation services,using a well-prepared protocol,with physician-managed anticoagulation services・Methods:A retrospective prospective pilot study was conducted to compare patient outcomes before and after transitioning patients to phannacist-managed anticoagulation services,comparing the proportion of those with therapeutic international normalized ratio(INK),subtherapeutic INK,and supratherapeutic INR,as well as their bleeding occurrences as indicators of assuring quality ***:A significant improvement in anticoagulation management was noted in the transition to pharmacist-managed anticoagulation *** proportion of those with subtherapeutic INR decreased from 61.8%to 11.8%(pupratherapeutic INR decreased from 20.6%to 2.9%(putic INR increased from 17.6%to 85.3%(purrence of bleeding decreased from 11.8%to 0.0%,without significant difference in warfarin doses(median from 4 before the transition to 5 after);in addition,the time to reach therapeutic INR decreased from 12-24 weeks to 2-8 weeks after transitioning to pharmacist-managed anticoagulation ***:Pharmacist-managed anticoagulation services are considered safer and more effective than physician-managed anticoagulation services alone in terms of patientsJ adherence and satisfaction,which provide an excellent opportunity for quality assurance care.

关键词： Pharmacist-managed anticoagulation services Warfarin Anticoagulation clinic Therapeutic drug monitoring

Ly49 receptors activate angiogenic mouse DbA＋ uterine natural killer cells

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Cellular & Molecular Immunology 2014年第5期11卷 467-476页

作者： Patricia DA Lima Megan M Tu Mir Munir A Rahim Annie R Peng b Anne Croy Andrew P Makrigiannis Department of biochemistry Microbiologyand Immunology University of Ottawa Ottawa ON KIN 6N5 Canada biomedical and Molecular Sciences Queen's University Kingston ON K7L 3N6 Canada

In humans, specific patterns of killer immunoglobulin-like receptors （kirs） expressed by uterine natural killer （uNK） cells are linked through HLA-C with pregnancy complications （infertility, recurrent spontaneous abortion, intrauterine growth restriction and preeclampsia）. To identify mechanisms underpinning the associations between NK cell activation and pregnancy success, pregnancies were studied in mice with genetic knockdown （KD） of the MHC-activated Ly49 receptor gene family. ***49KD pregnancies were compared to normal control ***49z29 and C57bL/6 （b6） pregnancies. At mid-pregnancy （gestation day （gd9.5））, overall uNK cell （TCRI^-CD122＋DbA＋DX5- （DbA＋DX5-）） and TCRIβ-CD122＋DbA-DX5＋（DbA-DX5＋）） frequencies in pregnant uterus were similar between genotypes. Ly49KD lowered the normal frequencies of Ly49＋ uNK cells from 90.3% to 47.8% in DbA-DX5＋ and 78.8% to 6.3% in DbA＋DX5- uNK cell subtypes. ***49KD matings frequently resulted in expanded blastocysts that did not implant （subfertility）. ***49KD mice that established pregnancy had gestational lengths and litter sizes similar to controls. ***49KD neonates, however, were heavier than controls. ***49KD implantation sites lagged in early （gd6.5） decidual angiogenesis and were deficient in mid-pregnancy （gd 10.5） spiral arterial remodelling. ultrastructural analyses revealed that ***49KD uNK cells had impaired granulogenesis, while immunocytochemistry revealed deficient vascular endothelial cell growth factor （VEGFA） production. Perforin and IFNG expression were normal in ***49KD uNK cells. Thus, in normal mouse pregnancies, Ly49 receptor signaling must promote implantation, early decidual angiogenesis and mid-pregnancy vascular remodelling. Disturbances in these functions may underlie the reported genetic associations between human pregnancy complications and the inability of specific conceptus MHCs to engage activating kir on uNK cells.

关键词： fetal growth deviation granule biogenesis MHC-I receptors uterine natural killer cells vascular endothelialcell growth factor

湖南汉族1型糖尿病患者杀伤细胞免疫球蛋白样受体及其配体基因多态性

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中华医学杂志 2010年第4期90卷 236-240页

作者：谭愈昱林健黄干唐炜立 Saikiran K Sedimbi Carani b Sanjeevi 周智广中南大学湘雅二医院代谢内分泌研究所、中南大学糖尿病中心、中南大学代谢综合征研究中心长沙410011 999027 Sweden Molecular Immunogenetics Division Department of Molecular Medicine and Surgery Center for Molecular Medicine Karolinska University Hospital

目的探讨杀伤细胞免疫球蛋白样受体（kir）基因和kir配体人类白细胞抗原-C（HLA—C）基因的多态性与1型糖尿病（T1DM）的关系。方法采用聚合酶链反应-序列特异性引物技术（PCR—SSP），对湖南汉族180例TIDM患者和199例正常对照者kir基因... 详细信息

目的探讨杀伤细胞免疫球蛋白样受体（kir）基因和kir配体人类白细胞抗原-C（HLA—C）基因的多态性与1型糖尿病（T1DM）的关系。方法采用聚合酶链反应-序列特异性引物技术（PCR—SSP），对湖南汉族180例TIDM患者和199例正常对照者kir基因、HIA—C基因进行检测和分析。结果（1）与正常对照相比，T1DM组kir2DL1（98．9％比92．0％，OR=7．78，P=0．002）、3DL1（94．3％比86．4％，OR=2．67，P=0．009）和2DS4（83．9％比70．9％，OR=2．14，P=0．003）基因频率增高；（2）T1DM组和正常对照组HIJA—c（HIJA—C1、HLA—C2）基因频率差异无统计学意义，但T1DM组HLAC1＋／C2＋（3．9％比9．6％，OR=0．38，P=0．03）基因频率降低；（3）与正常对照组相比，T1DM组kir／HLA－C基因组合中kir2DL1-／HLA－C2－（0．6％比6．0％，OR=0．087，P=0．003）和kir2DS1-／HLA—C2－（53．3％比64．8％，OR=0．62，P=0．023）基因频率降低。结论kir基因多态性和kir／HLA—C基因组合与T1DM发病相关。

关键词：糖尿病,1型人白细胞抗原杀伤细胞基因

Genome assembly of the snow lotus species Saussurea involucrata provides insights into acacetin and rutin biosynthesis and tolerance to an alpine environment

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Horticulture Research 2023年第10期10卷 8-11页

作者： Yanxia Sun Aidi Zhang Jacob bLandis Wei Shi Xiujun Zhang Hang Sun Hengchang Wang CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074HubeiChina Center of Conservation biology Core Botanical GardensChinese Academy of SciencesWuhan 430074HubeiChina Center of Economic botany Core Botanical GardensChinese Academy of SciencesWuhan 430074HubeiChina bTI Computational biology Center Boyce Thompson InstituteIthacaNY 14853USA School of Integrative Plant Science Section of Plant Biology and the L.H.Bailey HortoriumCornell UniversityIthacaNY 14853USA Xinjiang Institute of Ecology and Geography Chinese Academy of SciencesUrumchi 830011XinjiangChina Key Laboratory for Plant Diversity and biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina

Dear Editor,understanding the evolution and survival mechanisms of endangered wild medicinal herbs is crucial for their cultivation,utilization,and *** snow lotus species Saussurea involucrata(Kar.&kir.)***.(2n=32)(*** well-known Tianshan snow lotus)which belongs to the eudicot family Asteraceae,is a famous traditional Chinese medicinal herb having anti-inflammatory.

关键词： Tianshan belongs involucrata

HLA相合亲缘供受者自然杀伤细胞免疫球蛋白样受体基因的分析

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中国实验血液学杂志 2007年第6期15卷 1276-1280页

作者：朱发明姜侃吕沁风章伟何吉许先国严力行浙江省血液中心

本研究分析HLA相合亲缘关系供受者自然杀伤细胞免疫球蛋白样受体(kir)基因分布情况并探讨其kir相合的可能性。采用Luminex流式技术和测序方法检测HLA-A,-b,-Cw和-DRb1位点;利用序列特异性引物PCR法(PCR-SSP)检测kir基因。结果表明:在HLA... 详细信息

本研究分析HLA相合亲缘关系供受者自然杀伤细胞免疫球蛋白样受体(kir)基因分布情况并探讨其kir相合的可能性。采用Luminex流式技术和测序方法检测HLA-A,-b,-Cw和-DRb1位点;利用序列特异性引物PCR法(PCR-SSP)检测kir基因。结果表明:在HLA-A,-b,-Cw和-DRb1等8个位点相合的27对亲缘关系供受者样本中检出17个kir基因,其中kir3DL3、kir3DP1、kir2DL4和kir3DL2基因存在于所有个体;检出20种kir基因型和12种单倍体,常见基因型为2,2,频率为29.6%;最常见单倍体是2,频率为53.0%,样本以A型单倍体为主,频率为67.2%;12对(44.4%)供者kir与受者kir基因型和单倍体完全相同,13对(48.1%)供者kir与受者kir存在1个单倍体相同,2对(7.4%)供者kir与受者kir基因型和单倍体完全不同;1对供者kir2DL1与受者HLA-Cw(Lys80)配基完全相合,17对供者kir2DL2/2DL3与受者HLA-Cw(Asn80)配基完全相合,5对供者kir3DL1与受者HLA-b(bw4)配基完全相合。结论:HLA相合亲缘关系供受者kir匹配的错配率比较高。

关键词： HLA相合亲缘供受者自然杀伤细胞免疫球蛋白样受体 kir错配

浙江汉族人群杀伤细胞免疫球蛋白样受体基因多态性研究

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中国实验血液学杂志 2005年第6期13卷 1109-1112页

作者：朱发明姜侃吕沁风章伟张海琴傅启华严力行浙江省血液中心杭州310006

本研究的目的是分析浙江汉族人群杀伤细胞免疫球蛋白样受体(kir)基因的多态性及基因型。采用序列特异性引物PCR法(PCRSSP)进行kir基因低分辨率检测和基因型分析。结果表明:①在浙江汉族人群中共检出目前已知的17个kir基因。2DL4、3DL2和... 详细信息

本研究的目的是分析浙江汉族人群杀伤细胞免疫球蛋白样受体(kir)基因的多态性及基因型。采用序列特异性引物PCR法(PCRSSP)进行kir基因低分辨率检测和基因型分析。结果表明:①在浙江汉族人群中共检出目前已知的17个kir基因。2DL4、3DL2和3DL3基因存在于所有个体,其基因频率为1.00。2DL1、2DL3、2DP1、3DP1003、3DL1、2DS4001/002基因较为常见,频率分别为0.902、0.902、0.902、0.902、0.7598、0.5615;而2DL2、2DL5A、2DL5b、2DS1、2DS2、2DS3、2DS4003、2DS5、3DS1和3DP1001/002基因频率较低。②浙江汉族人群中以A型单倍型为主,频率高达74.7%。在检测样本中共明确检出12种单倍型,最常见的是单倍型2,频率为53.0%。③共检出26种基因型,最常见为AJ(2,2)、AF(1,2)型,其次为AH(2,5)、NN2(2,6)、AI(1,5)和AG(1,1)。其中有15种基因型在白人中尚未见报道,4种基因型无法根据相关文献标准分析出单倍型。结论:浙江汉族人群有其独特的kir基因频率和基因型频率分布。

关键词：杀伤细胞免疫球蛋白样受体单倍型基因多态性

分离人杀伤细胞抑制受体NKAT2特异的单链抗体

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暨南大学学报（自然科学与医学版） 1998年第6期19卷 51-52页

作者：乔旭刚 Colon.,M 暨南大学医学院血液病研究室

人自然杀伤细胞表面的抑制受体（ＫＩＲ），是与表达在其他细胞表面的相应ＨＬＡＩ类分子相互作用的。许多ＫＩＲ受体已被克隆，小部分量的单克隆抗体也已产生。然而为区分不同位点及其等位基因的产物，并精确阐明其功能，需要更多的研... 详细信息

人自然杀伤细胞表面的抑制受体（ＫＩＲ），是与表达在其他细胞表面的相应ＨＬＡＩ类分子相互作用的。许多ＫＩＲ受体已被克隆，小部分量的单克隆抗体也已产生。然而为区分不同位点及其等位基因的产物，并精确阐明其功能，需要更多的研究试剂。本研究探讨用噬菌体抗体库，分离ＫＩＲ单链抗体（ｓｃＦｖ）的可行性。方法：ＮＫＡＴ２－ＩｇＧ１为一ＫＩＲ，与人ＩｇＧ１Ｆｃ以融合蛋白形成表达而获得。噬菌体抗体库为“Ｎｉｓｉｍ”抗体库。抗体库ＮＫＡＴ２－ＩｇＧ１包被的免疫管５次筛选。ｓｃＦｖ由特异的噬菌体感染细菌，诱导而得。筛选效应经噬体菌体的菌落形成单位及ＤＮＡ酶切图谱检测。筛选的克隆进行ＤＮＡ序列分析。ｓｃＦｖ的特异性及免疫学特征由ＥＬＩＳＡ、聚丙烯胺凝胶电泳及免疫印迹分析。细胞结合实验由流式细胞仪测试。结果：５次筛选后，ＮＫＡＴ２特异的噬菌体达到２００倍的富集，ＤＮＡ酶切图谱也显示某种特定消化类型的富集。经ＥＬＩＳＡ分析，结果显示３７个克隆与ＮＫＡＴ２反应呈强阳性。这些克隆与ＮＫＡＴ１－ＩｇＧ１、ＮＫＡＴ３－ＩｇＧ１、ＮＫＡＴ４－ＩｇＧ１、人ＩｇＧ及其他非相关蛋白的反应均为阴性。其中一个克隆（ｓｃＨＵＢ１）经流式细胞仪进一步测试，显示?

关键词：杀伤细胞分离抗体抑制受体 NKAT2 单链抗体