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Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023 edition)

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Chinese herbal Medicines 2024年第1期16卷 3-12页

作者： Qing Zhao tong Wang Hongbin Wang Peng Cao Chengyu Jiang Hongzhi Qiao Lihua Peng Xingdong Lin Yunyao Jiang Honglei Jin Huantian Zhang Shengpeng Wang Yang Wang Ying Wang Xi Chen Junbing Fan Bo Li Geng Li Bifeng Liu Zhiyang Li Suhua Qi Mingzhen Zhang Jianjian zheng Jiuyao Zhou Lei zheng Kewei Zhao Guangdong Engineering Research Center of Chinese herbal Vesicles The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Institute of Life and health Engineering the First Affiliated HospitalJinan UniversityGuangzhou 510630China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210046China Institute of Basic Medicine Chinese Academy of Medical SciencesBeijing 100005China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310030China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Department of Bone and Joint Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou 510630China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAomen 999078China Shanghai Jiao tong University Affiliated Sixth People’s Hospital Shanghai 200233China School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515China School of Life Sciences Nanjing UniversityNanjing 210008China Nanfang Hospital Southern Medical UniversityGuangzhou 510515China Laboratory Animal Center Guangzhou University of Chinese MedicineGuangzhou 510405China College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital Nanjing 210008China School of Medical and Technology Xuzhou Medical UniversityXuzhou 221004China School of Basic Medical Sciences Xi’an Jiaotong UniversityXi’an 710065China The First Affiliated Hospital of Wenzhou Medical University WenZhou 325035China

To promote the development of extracellular vesicles of herbal medicine especially the establishment of standardization,led by the National Expert Committee on Research and Application of Chinese herbal Vesicles,research experts in the field of herbal medicine and extracellular vesicles were invited nationwide with the support of the Expert Committee on Research and Application of Chinese herbal Vesicles,Professional Committee on Extracellular Vesicle Research and Application,Chinese Society of Research Hospitals and the Guangdong Engineering Research Center of Chinese herbal *** on the collation of relevant literature,we have adopted the Delphi method,the consensus meeting method combined with the nominal group method to form a discussion draft of“Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023)”.The first draft was discussed in online and offline meetings on October 12,14,November 2,2022 and April and May 2023 on the current status of research,nomenclature,isolation methods,quality standards and research applications of extracellular vesicles of Chinese herbal medicines,and 13 consensus opinions were finally *** the Third Academic Conference on Research and Application of Chinese herbal Vesicles,held on May 26,2023,Kewei Zhao,convenor of the consensus,presented and read the consensus to the experts of the Expert Committee on Research and Application of Chinese herbal *** consensus highlights the characteristics and advantages of Chinese medicine,inherits the essence,and keeps the righteousness and innovation,aiming to provide a reference for colleagues engaged in research and application of Chinese herbal vesicles at home and abroad,decode the mystery behind Chinese herbal vesicles together,establish a safe,effective and controllable accurate Chinese herbal vesicle prevention and treatment system,and build a bridge for Chinese medicine to the world.

关键词： Chinese herbal medicine extracellular vesicles research application consensus

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An engineered Cas12i nuclease that is an efficient genome editing tool in animals and plants

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The Innovation 2024年第2期5卷 61-69页

作者： Zhiqiang Duan Yafeng Liang Jialei Sun Hongjin zheng tong Lin Pengyu Luo Mengge Wang Ruiheng Liu Ying Chen Shuhua Guo Nannan Jia Hongtao Xie Meili Zhou Minghui Xia Kaijun Zhao Shuhui Wang Na Liu Yongling Jia Wei Si Qitong Chen Yechun Hong Ruilin Tian Jian-Kang Zhu Institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China Bellagen Biotechnology Jinan 250000China Department of Medical Neuroscience School of MedicineKey University Laboratory of Metabolism and Health of GuangdongSouthern University of Science and TechnologyShenzhenGuangdong Province 518055China

The type V-I CRISPR-Cas system is becoming increasingly more attractive for genome ***,natural nucleases of this system often exhibit low efficiency,limiting their ***,we used structure-guided rational design and protein engineering to optimize an uncharacterized Cas12i nuclease,*** a result,we developed Cas-SF01,a Cas12i3 variant that exhibits significantly improved gene editing activity in mammalian ***-SF01 shows comparable or superior editing performance compared to SpCas9 and other Cas12 *** to natural Cas12i3,Cas-SF01 has an expanded PAM range and effectively recognizes NTTN and noncanonical NATN and TTVN *** addition,we identified an amino acid substitution,D876R,that markedly reduced the off-target effect while maintaining high on-target activity,leading to the development of CasSF01^(HiFi)(high-fidelity Cas-SF01).Finally,we show that Cas-SF01 has high gene editing activities in mice and *** results suggest that CasSF01 can serve as a robust gene editing platform with high efficiency and specificity for genome editing applications in various organisms.

关键词： system attractive rational

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级配碎石层协调沥青混合料层温度收缩变形行为

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吉林大学学报（工学版） 2024年第4期54卷 1045-1057页

作者：万铜铜汪海年郑文华冯珀楠陈玉张琛长安大学特殊地区公路工程教育部重点实验室西安710064 西安航空学院能源与建筑学院西安710077

为揭示级配碎石基层与沥青混合料面层的温度收缩协调变形规律,本文采用动静态应变采集系统实时获取沥青混合料层的温缩应变,基于PFC6.0 Suite和FLAC3D构建了组合结构连续-离散耦合模型,探究了级配碎石层与沥青混合料层温缩协调变形宏观... 详细信息

为揭示级配碎石基层与沥青混合料面层的温度收缩协调变形规律,本文采用动静态应变采集系统实时获取沥青混合料层的温缩应变,基于PFC6.0 Suite和FLAC3D构建了组合结构连续-离散耦合模型,探究了级配碎石层与沥青混合料层温缩协调变形宏观和细观响应规律。研究结果表明:连续-离散耦合模型与室内试验的吻合度相比连续介质模型高,温缩系数相对误差最大为8.1%;沥青混合料及其组合试件温缩应变-时间关系曲线均呈先快后慢的非线性变化规律,沥青混合料类型及降温温差对级配碎石的约束作用基本无影响;组合试件温缩变形主要发生在前1 h内,沥青混合料层整体由“翘起”状逐渐过渡到“平缓”状;级配碎石层通过颗粒向内挤压运动,使两端松散膨胀及颗粒接触重组来实现与沥青混合料层的协调变形。本文从宏观应变响应与空隙率、配位数及三维组构细观响应揭示温缩协调变形机制,研究结果可为级配碎石基层沥青路面低温抗裂研究提供理论参考。

关键词：道路工程协调变形连续-离散耦合温缩开裂级配碎石细观响应

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黄河(郑州段)水体中微塑料污染特征及来源分析

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中国环境科学 2024年第7期44卷 4136-4144页

作者：李和通马振芳郭宇邓正云李银月桂新马丽张发文河南省郑州生态环境监测中心河南郑州450007 河南农业大学河南郑州450046

为探究微塑料在我国黄河中下游水体中的分布情况和污染特征,以黄河(郑州段)为研究对象,选取7个具有代表性的点位采集水样,通过体视显微镜对微塑料的丰度和形貌特征进行观察,进一步借助显微红外光谱对其组成成分进行鉴定,并将统计结果与... 详细信息

为探究微塑料在我国黄河中下游水体中的分布情况和污染特征,以黄河(郑州段)为研究对象,选取7个具有代表性的点位采集水样,通过体视显微镜对微塑料的丰度和形貌特征进行观察,进一步借助显微红外光谱对其组成成分进行鉴定,并将统计结果与其他水体的微塑料污染状况进行对比分析,并探究其污染来源.结果表明:黄河(郑州段)水体中微塑料平均丰度为(16.86±4.20)n/L;以粒径小于1mm的尺寸为主,占比高达61.42%;形状中纤维状占比最高为79.31%;微塑料颜色以透明为主,占比为56.06%;微塑料聚合物类型以人造丝(Rayon)为主,同时还有聚对苯二甲酸乙二醇酯(PET)和聚丙烯(PP)等.不同点位微塑料的形貌特征和聚合物类型可在一定程度上反映其潜在污染来源.黄河(郑州段)和其他水体相比,微塑料丰度分布存在一定的差异性,粒径分布趋势则大致相同,形状、颜色以纤维状、透明为主,不同水体间的微塑料污染差异可能与水体周边生境、经济水平以及人类活动情况等因素密切相关.

关键词：微塑料污染特征黄河丰度分布

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Esophageal ulcer and multisystem inflammatory syndrome after COVID-19:A case report

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World Journal of Gastrointestinal Endoscopy 2024年第8期16卷 483-488页

作者： Ni Yang Zhen Liu tong Jin Hai-Wei Xin Li Gu Yue zheng Hui-Xing Zhou Ning Li Xin-Juan Liu Department of Gastroenterology Beijing Chao-Yang HospitalCapital University of Medical ScienceBeijing 100020China Department of Infectious Diseases and Clinical Microbiology Beijing Chao-Yang HospitalCapital University of Medical ScienceBeijing 100020China Department of Surgical Intensive Care Unit Beijing Chao-Yang HospitalCapital University of Medical ScienceBeijing 100020China Department of hematology Beijing Chao-Yang HospitalCapital University of Medical ScienceBeijing 100020China

BACKGROUND Multisystem inflammatory syndrome in adults(MIS-A)is a rare but severe disease occurring several weeks after severe acute respiratory syndrome coronavirus 2 *** develops in adults with inflammation of different organs including the gastrointestinal tract,heart,kidneys,skin and hematopoietic *** SUMMARY We present a 58-year-old Chinese man diagnosed with *** chief complaints were fever,generalized fatigue and anorexia,accompanied with rashes on his *** examination showed cardiac,renal and liver *** had melena and gastroscopy indicated esophageal ulcer and severe *** blood and sputum culture did not show growth of bacteria or *** treatment was stopped due to unsatisfactory *** condition improved after prednisone and other supportive *** Gastrointestinal involvement in MIS-A is not *** involvement predominates,and esophageal involvement is rarely *** ulcer with bleeding could also be a manifestation of MIS-A.

关键词： Multisystem inflammatory syndrome Esophageal ulcer SARS-CoV-2 Gastrointestinal bleeding Case report

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Real-world effectiveness of early insulin therapy on the incidence of cardiovascular events in newly diagnosed type 2 diabetes

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2953-2959页

作者： Sihui Luo Xueying zheng Wei Bao Sheng Nie Yu Ding tong Yue Yilun Zhou Ying Hu Hua Li Qiongqiong Yang Qijun Wan Bicheng Liu Hong Xu Guisen Li Gang Xu Chunbo Chen Huafeng Liu Yongjun Shi Yan Zha Yaozhong Kong Guobin Su Ying Tang Mengchun Gong Linong Ji Fan Fan Hou Jianping Weng Department of Endocrinology First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHeifeiChina Institute of Public health Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiChina Division of Nephrology National Clinical Research Center for Kidney DiseaseState Key Laboratory of Organ Failure ResearchNanfang HospitalSouthern Medical UniversityGuangzhouChina Department of Nephrology Beijing Tiantan HospitalCapital Medical UniversityBeijingChina The Second Affiliated Hospital of Zhejiang University School of Medicine HangzhouChina Sir Run Run Shaw Hospital Zhejiang University School of MedicineHangzhouChina Department of Nephrology Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhouChina The Second People’s Hospital of Shenzhen Shenzhen UniversityShenzhenChina Institute of Nephrology Zhongda HospitalSoutheast University School of MedicineNanjingChina Children’s Hospital of Fudan University ShanghaiChina Renal Department and Institute of Nephrology Sichuan Provincial People’s HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaSichuan Clinical Research Center for Kidney DiseasesChengduChina Division of Nephrology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Critical Care Medicine Maoming People’s HospitalMaomingChina Key Laboratory of Prevention and Management of Chronic Kidney Disease of Zhanjiang City Institute of NephrologyAffiliated Hospital of Guangdong Medical UniversityZhanjiangChina Huizhou Central People’s Hospital Sun Yat-Sen UniversityHuizhouChina Guizhou Provincial People’s Hospital Guizhou UniversityGuiyangChina Department of Nephrology the First People’s Hospital of FoshanFoshanChina Department of Nephrology Guangdong Provincial Hospital of Chinese Medicinethe Second Affiliated Hospitalthe Second Clinical CollegeGuangzhou University of Chinese Medicin

Early insulin therapy is capable to achieve glycemic control and restoreβ-cell function in newly diagnosed type 2 diabetes(T2D),but its effect on cardiovascular outcomes in these patients remains *** this nationwide real-world study,we analyzed electronic health record data from 19 medical centers across China between 1 January 2000,and 26 May *** included 5424 eligible patients(mean age 56 years,2176 women/3248 men)who were diagnosed T2D within six months and did not have prior cardiovascular *** Cox regression models were used to estimate the associations of early insulin therapy(defined as the first-line therapy for at least two weeks in newly diagnosed T2D patients)with the incidence of major cardiovascular events including coronary heart disease(CHD),stroke,and hospitalization for heart failure(HF).During 17,158 persons years of observation,we documented 834 incident CHD cases,719 stroke cases,and 230 hospitalized cases for *** diagnosed T2D patients who received early insulin therapy,compared with those who did not receive such treatment,had 31%lower risk of incident stroke,and 28%lower risk of hospitalization for *** significant difference in the risk of CHD was *** found similar results when repeating the aforesaid analysis in a propensity-score matched population of 4578 patients and with inverse probability of treatment weighting *** findings suggest that early insulin therapy in newly diagnosed T2D may have cardiovascular benefits by reducing the risk of incident stroke and hospitalization for HF.

关键词： cardiovascular treatment nationwide

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Zr^(4+)共掺对Cs_(2)LaLiBr_(6):Ce晶体的中子/伽马甄别性能的影响

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无机材料学报 2024年第5期39卷 539-546页

作者：郑中秋魏钦华童宇枫唐高尹航秦来顺中国计量大学材料与化学学院杭州310018 中国科学院上海硅酸盐研究所人工晶体研究中心上海201899

中子探测技术广泛用于国土安全、核材料安全检测以及高能物理等领域,由于3he资源紧缺,近年来急需开发出能够同时甄别中子/伽马的新型闪烁晶体,Cs_(2)LaLiBr_(6):Ce(CLLB:Ce)晶体具有良好的中子/伽马甄别能力、优异的能量分辨率以及高的... 详细信息

中子探测技术广泛用于国土安全、核材料安全检测以及高能物理等领域,由于3he资源紧缺,近年来急需开发出能够同时甄别中子/伽马的新型闪烁晶体,Cs_(2)LaLiBr_(6):Ce(CLLB:Ce)晶体具有良好的中子/伽马甄别能力、优异的能量分辨率以及高的光输出,但其中子/伽马甄别性能有待进一步提高。本研究采用垂直布里奇曼法成功生长了Zr^(4+)共掺杂的CLLB:Ce晶体。通过不同表征手段研究了Zr^(4+)共掺杂CLLB:Ce晶体的结构和组分,结果表明Zr^(4+)成功掺入基质材料且对基质晶体结构不产生明显的影响,Zr^(4+)共掺杂后没有产生新的发光中心,紫外衰减时间约为27.0 ns,仍具有较快的荧光衰减。Zr^(4+)共掺杂CLLB:Ce晶体的品质因子(Figure of Merit,FOM)从1.2提高到1.5,表明其中子/伽马甄别能力得到改善。结合热稳定性和闪烁衰减时间,探讨了衰减时间对FOM的影响机制,Zr^(4+)共掺杂可以抑制浅电子陷阱和Vk中心,减少电子捕获和脱陷过程,使Ce^(3+)直接捕获的概率大大增加,从而表现出更快的衰减速率。本研究显示,Zr^(4+)共掺杂CLLB:Ce晶体在中子/伽马探测领域具有潜在的应用前景。

关键词：闪烁晶体 CLLB:Ce 中子/伽马甄别钾冰晶石结构

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多蛇形机器人编队路径跟踪控制

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电子与信息学报 2024年第7期46卷 2981-2993页

作者：郝爽何玉鹏陈继尧王铮上海交通大学安泰经济与管理学院上海200030 国网宁夏电力有限公司银川750001 国网宁东供电公司银川750411 国网宁夏电力有限公司经济技术研究院银川750002

为了实现多个蛇形机器人的编队控制,该文提出一种基于误差约束的抗干扰路径跟随方法。该方法使用高度耦合的动态频率补偿器来调整每个机器人的运动速度,以确保编队成员之间位置和速度的一致性。在动力学控制中,通过障碍函数的等效原则... 详细信息

为了实现多个蛇形机器人的编队控制,该文提出一种基于误差约束的抗干扰路径跟随方法。该方法使用高度耦合的动态频率补偿器来调整每个机器人的运动速度,以确保编队成员之间位置和速度的一致性。在动力学控制中,通过障碍函数的等效原则消除了虚拟变量的奇异现象,提高了路径跟随的稳定性。此外,该文设计了模型不确定性和外界干扰的预测值,以此来提前补偿机器人的关节偏移量和扭矩输入,从而进一步提高了跟随误差的收敛速度和稳态性能。最后,利用Lyapunov理论证明了该方法的一致最终有界性(UUB)。仿真数据表明,相对于其他经典方法,该文所提模型和控制策略具有更高的跟随精度。

关键词：抗干扰障碍李雅普诺夫函数误差约束编队控制多蛇形机器人

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Defect reduction to enhance the mechanical strength of nanocellulose carbon aerogel

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Chinese Chemical Letters 2024年第1期35卷 492-498页

作者： Haihong Lai Zehong Chen Hao Zhuo Yijie Hu Xuan Zhao Jiwang Yi Hongzhi zheng Ge Shi Yifan tong Ling Meng Xinwen Peng Linxin Zhong State Key Laboratory of Pulp and Paper Engineering South China University of TechnologyGuangzhou 510641China Huangpu Hydrogen Energy Innovation Center/Guangzhou Key Laboratory for Clean Energy and Materials School of Chemistry and Chemical EngineeringGuangzhou UniversityGuangzhou 510006China

Carbon aerogels prepared from renewable nano building blocks are rising-star materials and hold great promise in many ***,various defects formed during carbonization at high temperature disfavor the stress transfer and thus the fabrication of flexible carbon aerogel from renewable nano building ***,a structural defect-reducing strategy is proposed by altering the pyrolysis route of cellulose *** salt that inhibits the generation of tar volatilization during pyrolysis can prevent the formation of various structural *** with fewer defects can reduce stress concentration and remarkably enhance the compressibility of carbon aerogel,thus increasing the maximum stress retention of carbon *** carbon aerogel also has high stress sensor sensitivity and excellent temperature coefficient of *** structural defect-reducing strategy will pave a new way to fabricate high-strength carbon materials for various fields.

关键词： Biomass Cellulose nanofiber Pyrolysis Renewable Carbon aerogel

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Numerical and experimental study of the effects of wind turbine operation on sand-dust transport characteristics

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 97-113页

作者： Gaosheng Ma Hong Han Ye Li Deshun Li Yan Wang Ning Fu Quan zheng Rennian Li School of Energy and Power Engineering Lanzhou University of TechnologyLanzhou 730050China Gansu Provincial Technology Center for Wind Turbines Lanzhou 730050China Key Laboratory of Fluid Machinery and Systems Lanzhou 730050China Multiple Function Towing Tank Laboratory State Key Laboratory of Ocean EngineeringSchool of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Given factors such as reduced land availability for onshore wind farms,wind resource enrichment levels,and costs,there is a growing trend of establishing wind farms in deserts,the Gobi,and other arid ***,the relationship between sanddust weather environments and wind turbine operations has garnered significant *** investigate the impact of wind turbine wakes on sand-dust transportation,this study employs large eddy simulation to model flow fields,coupled with an actuator line model for simulating rotating blades and a multiphase particle in cell model for simulating sand *** research focuses on a horizontal axis wind turbine model and examines the motion and spatiotemporal distribution characteristics of four typical sizes of sand particles in the turbine *** findings reveal that sand particles of varying sizes exhibit a spiral settling pattern after traversing the rotating plane of wind turbine blades,influenced by blade shedding vortex and *** particles tend to cluster in the peripheries of the vortex cores of low vorticity in the wind turbine *** rotation of wind turbines generates a wake vortex structure that causes a significant clustering of sand particles at the tip *** the wake distance increases,the particles that cluster at the turbine's tip gradually spread outward to approximately twice the rotor diameter and then begin to mix with the incoming flow *** turbines have a noticeable impact on sand-dust transportation,hindering their movement to a significant *** average sand-blocking rate exhibits a trend of initially increasing and then decreasing as the wake distance *** its peak,the sand-blocking rate reaches an impressive 67.55%.The presence of wind turbines induces the advanced settling of sand particles,resulting in a“triangular”distribution of the deposition within the ground projection area of the wake.

关键词： wind turbine particle transportation actuator line model dry deposition particle clustering

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