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Expert consensus on the diagnosis and treatment of macrolide-resistant Mycoplasma pneumoniae pneumonia in children

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World Journal of pediatrics 2024年第9期20卷 901-914页

作者： Ying-Shuo Wang Yun-Lian Zhou Guan-Nan Bai Shu-Xian Li dan Xu Li-Na Chen Xing Chen Xiao-Yan Dong hong-Min fu Zhou fu Chuang-Li Hao Jian-Guo hong En-Mei liu Han-Min liu Xiao-Xia Lu Zheng-Xiu Luo Lan-Fang Tang Man Tian Yong Yin Xiao-Bo Zhang Jian-Hua Zhang Hai-Lin Zhang De-Yu Zhao Shun-Ying Zhao Guo-hong Zhu Ying-Xue Zou Quan Lu Yuan-Yuan Zhang Zhi-Min Chen Department of pulmonology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Child Health Care Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of pediatric pulmonology and Immunology West China Second University HospitalSichuan UniversityChengdu 610041China Department of pediatrics Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China Department of pulmonology Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200062China Department of pulmonary and Critical Care Medicine Kunming Children’s HospitalKunming 650034China Department of respiratory Medicine Children’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of pulmonology Children’s Hospital of Soochow UniversitySuzhou 215003China Department of pediatrics Shanghai General Hospital Affiliated to Shanghai Jiaotong University School of MedicineShanghai 200080China Department of respiratory Medicine Wuhan Children’s HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430015China Department of respiratory Medicine Children’s Hospital of Nanjing Medical UniversityNanjing 210008China Department of respiratory Medicine Shanghai Children’s Medical CenterShanghai Jiao Tong University School of MedicineShanghai 200127China Department of respiratory Medicine Children’s Hospital of Fudan UniversityShanghai 201102China Department of pediatric pulmonology Xinhua HospitalShanghai Jiaotong University School of MedicineShanghai 201102China Department of pediatric respiratory Medicine The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou 325027China National Clinical research Center for respiratory Diseases Beijing Children’s HospitalCapital Medical UniversityBeijing 100045China Departmen

Background Mycoplasma pneumoniae(***)is a significant contributor to community-acquired pneumonia among *** 1968,when a strain of *** resistant to macrolide antibiotics was initially reported in Japan,macrolide-resistant ***(MrMp)has been documented in many countries worldwide,with varying incidence *** infections lead to a poor response to macrolide antibiotics,frequently resulting in prolonged fever,extended antibiotic treatment,increased hospitalization,intensive care unit admissions,and a significantly higher proportion of patients receiving glucocorticoids or second-line *** 2000,the global incidence of MrMp has gradually increased,especially in East Asia,which has posed a serious challenge to the treatment of *** infections in children and attracted widespread attention from ***,there is still no global consensus on the diagnosis and treatment of MrMp in *** We organized 29 Chinese experts majoring in pediatric pulmonology and epidemiology to write the world’s first consensus on the diagnosis and treatment of pediatric MrMp pneumonia,based on evidence *** evidence searches and reviews were conducted using electronic databases,including pubMed,Embase,Web of Science,CNKI,Medline,and the Cochrane *** used variations in terms for“macrolide-resistant”,“Mycoplasma pneumoniae”,“Mp”,“***”,“pneumonia”,“MrMp”,“lower respiratory tract infection”,“Mycoplasma pneumoniae infection”,“children”,and“pediatric”.results Epidemiology,pathogenesis,clinical manifestations,early identification,laboratory examination,principles of antibiotic use,application of glucocorticoids and intravenous immunoglobulin,and precautions for bronchoscopy are *** and rapid identification of gene mutations associated with MrMp is now available by polymerase chain reaction and fluorescent probe techniques in respiratory *** the resistance rate to mac

关键词： Child Macrolide-resistant Mycoplasma pneumoniae recommendation Diagnosis Treatment

Autophagy Vitalizes Appressorium Morphogenesis in Magnaporthe oryzae

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Autophagy Vitalizes Appressorium Morphogenesis in Magnaporth...

A simple and effective method for total rNA isolation of appressoria in Magnaporthe oryzae

中国植物病理学会2008年学术年会

作者： Xiao-hong liu Jian-ping Lu tong-Bao liu fu-Cheng Lin Biotechnology Institute, zhejiang university, hangzhou 310029,p. r. china

One of the most widespread and devastating plant diseases is rice blast which causes significant crop losses throughout china,South East Asia,and South America,representing a loss of 157 million tons of rice per annum...

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 811-817页

作者： tong-bao liu Jian-ping LU Xiao-hong liu Hang MIN fu-cheng LIN State Key Laboratory for riee Biology Biotechnology Institute Zhejiang University Hangzhou 310029 China College of Life sciences Zhejiang University Hangzhou 310058 China

Appressorium formation is an important event in establishing a successful interaction between the rice blast fungus, Magnaporthe oryzae, and its host plant, rice. An understanding of molecular events occurring in appressorium differentiation will give new strategies to control rice blast. A quick and reliable method to extract total rNA from appressorium is essential for studying gene expression during appressorium formation and its mechanism. We found that duplicate film is an efficient substratum for appressorium formation, even when inoculated with high density conidia. When inoculated with conidia at 1 × 106 ml^-1, the percentages of conidium germination and appressorium formation were （97.98±0.67）% and （97.88±0.45）%, respectively. We applied Trizol before appressorium collection for total rNA isolation, and as much as 113.6 lag total rNA was isolated from the mature appressoria at 24 h after inoculation. functional analysis of two genes, MNH6 and MgATG1, isolated from the cDNA subtractive library, revealed that the quantity of rNA was good enough to construct a cDNA （complementary DNA） library or a cDNA subtractive library. This method may be also applicable for the appressorium rNA isolation of other pathogenic fungi in which conidia differentiate into appressoria in the early stages of host infection.

关键词： Appressorium Magnaporthe oryzae rNA isolation

MoFLp1,encoding a novel fungal fasciclin-like protein,is involved in conidiation and pathogenicity in Magnaporthe oryzae

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 434-444页

作者： tong-bao liu Guo-qing CHEN Hang MIN fu-cheng LIN State Key Laboratory for rice Biology Biotechnology Institute Zhejiang University Hangzhou 310029 China College of Life sciences Zhejiang University Hangzhou 310058 China

Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLp1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLp1 gene contains an open reading frame (OrF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLp1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GpI) anchor additional signal at its N- and C-terminuses, respectively. potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLp1. Sequence analysis and subcellular localization by the expression of MoFLp1-GFp fusion construct in M. oryzae indicated that the MoFLp1 protein is probably localized on the vacuole membrane. Two MoFLp1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal de-velopment and pathogenicity in M. oryzae.

关键词： Magnaporthe oryzae Fasciclin MoFLp1 Cellular localization Conidiation pathogenicity

proteomic Identification and function Analysis of the Silkworm,Bombyx mori

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Proteomic Identification and Function Analysis of the Silkwo...

Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts

2008年全国生物化学与分子生物学学术大会

作者： Yao-zhou Zhang~1 Qing-you Xia~2 Jie Xu~1 Ling-ying Kong~1 Jian Chen~1 Zuo-ming Nie~1 dan Wang~1 Jian-qing Chen~1 Qing-liang Zheng~1 Qing Chen~1 Xiao-yuan ren~1 Jiang Wang~1 Zheng-bing Lü~1 Cai-ying Jiang~1 Li-li liu~1 Qing Sheng Yong-feng Jin~3 Xiang-fu Wu~1 1 The Key Laboratory of Bioreactor and Biopharmacy of zhejiang province Institute of Biochemistry,zhejiang Sci-Tech university,hangzhou 310018,china 2 The Key Sericultural Laboratory of Agricultural Ministry The Key Laboratory for Sericultural sciences and Genomics of the Ministry of Education,College of Biotechnology,Southwest university,Beibei,Chongqing 400715,china 3 College of Life sciences,zhejiang university,hangzhou 310029,china

Mass spectrometry (MS) is an effective method for large-scale proteomic identification and verification of protein-encoding genes present in a *** this study,peptide sequences identified by MS were matched with those ...

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Insect Science 2022年第2期29卷 539-549页

作者： dan-tong Zhu Qiong rao Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng liu Ministry of Agriculture Key Lab of Molecular Biology of Crop pathogens and Insects Institute of Insect SciencesZhejiang UniversityHangzhou310029 China School of Agriculture and Food Science Zhejiang Agriculture and Forestry UniversityLin'anHangzhou311300 China

Nutritional mutualism between insects and symbiotic bacteria is *** various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(portiera)and multiple secondary *** is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect *** effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well *** examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the *** whitefly complex,provisionally named as Asia II 3 and china *** sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B *** then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously *** analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were *** findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host *** phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B *** metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution.

关键词： symbiont B vitamins complement evolution whitefly

Cloning,sequencing and expression analysis of the NAr promoter activated during hyphal stage of Magnaporthe grisea

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Journal of zhejiang university-Science B(Biomedicine & Biotechnology) 2007年第9期8卷 661-665页

作者： LU Jian-ping DUAN Zhi-bing liu tong-bao LIN fu-cheng Department of Biology School of Life Sciences Zhejiang University Hangzhou 310058 China Biotechnology Institute Zhejiang University Hangzhou 310029 China

The promoter of NAr gene in Magnaporthe grisea was isolated and sequenced. The promoter sequences contained the "TATA" box, the "CAAT" box, and binding sites for fungal regulatory proteins. programs that predict promoter sequences in-dicated that promoter sequence lies between locations 430 and 857 of the NAr promoter fragment. GFp expression under the NAr promoter and NAr transcript analysis revealed that this promoter is activated primarily at the mycelial stage in the rice blast fungus and could be used to express native or extrinsic genes in the mycelia of the rice blast fungus.

关键词： Magnaporthe grisea promoter NAr Clone Hypha

physiological and Molecular Features of the pathosystem Arabidopsis thaliana L.-Sclerotinia sclerotiorum Libert

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Journal of Integrative plant Biology 2006年第1期48卷 44-52页

作者： fu-Ming Dai tong Xu Gerhard A. Wolf Zu-Hua He College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Institute of plant physiology and Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China Institute of plant pathology Georg-August University Goettingen Goettingen 37077 Germany

The fungal pathogen Sclerotinia sclerotiorum Libert causes rot diseases on many crops worldwide and large economic losses occur frequently because of a lack of resistant varieties. The pathogenesis of S. sclerotiorum and the molecular basis of plant responses to the pathogen are poorly understood. In the present investigation, the process of S. sclerotiorum infection in Arabidopsis thaliana L., a plant that is highly susceptible to this fungus, was analysed. In addition, the defense activation in the host was investigated. A convenient inoculation method using millet grain was developed for S. sclerotiorum in Arabidopsis. The fungus rapidly infected the plants, probably through ball- or cushion-like infection structures. Visible symptoms developed within 24 h and plants were killed 72 h after inoculation. Cellulase, the main enzyme that caused host tissues to rot, was secreted by S. sclerotiorum in a pH-dependent manner. Oxalic acid, another pathogenic factor secreted by the fungus, induced necrotic lesions on the leaves, infection with S. sclerotiorum strongly induced the production of the pathogenesis-related （pr） proteins β-1,3-glucanase and chitinase in Arabidopsis. furthermore, the pr gene pDF. 1 was induced, but not pr1, indicating that the pathogen activated basal defense of jasmonic acid/ethylene dependence, which is consistent with its necrotrophic characteristics. This pathosystem for Arabidopsis-S. sclerotiorum could provide an approach for the analysis of the interactions between S. sclerotiorum and other crops, thereby facilitating genetic manipulation techniques for controlling this pathogen.

关键词： Arabidopsis defense pathogenesis pathosystem Sclerotinia sclerotiorum.

The real-world study of the clinical characteristics,diagnosis,and treatment of advanced pancreatic cancer in china

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Journal of pancreatology 2024年第1期7卷 1-9页

作者： Jiujie Cui Qihan fu Xiaobing Chen Yanling Wang Qi Li Feng Wang Zhihua Li Guanghai Dai Yusheng Wang hongmei Zhang Houjie Liang Jun Zhou liu Yang Fenghua Wang Leizhen Zheng Xiaofeng Chen ping Gong Jiang liu Ying Yuan Lin Wang Yuejuan Cheng Jun Zhang Yuhong Zhou Weijian Guo Xianbao Zhan Zhengyun Zou Da Li Shan Zeng Enxiao Li Zhiwei Li Zan Teng dan Cao Jie Kan Jianping Xiong Ming Quan Jiayu Yao Haiyan Yang Liwei Wang Department of Medical Oncology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Medical Oncology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouChina Department of Medical Oncology Affiliated Cancer Hospital of Zhengzhou UniversityHenan Cancer HospitalZhengzhouChina Department of Medical Oncology Shanghai General HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Medical Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Medical Oncology Sun Yat-sen Medical Hospital Sun Yat-sen UniversityGuangzhouChina Medical Oncology Chinese PLA General HospitalBeijingChina Department of Gastrointestinal Oncology Shanxi Provincial Cancer HospitalAffiliated to Shanxi Medical UniversityTaiyuanChina Department of Clinical Oncology Xijing HospitalAir Force Medical UniversityXi’anChina Department of Oncology and Southwest Cancer Center Southwest HospitalThird Military Medical University(Army Military Medical University)ChongqingChina Department of Gastrointestinal Oncology Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Peking University Cancer Hospital&InstituteBeijingChina Cancer Center Department of Medical OncologyZhejiang Provincial People’s HospitalPeople’s Hospital of Hangzhou Medical CollegeHangzhouChina Department of Medical Oncology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongChina Oncology Department Xin Hua Hospital Affiliated to Shanghai JiaoTong University School of MedicineShanghaiChina Department of Oncology the First Affiliated Hospital with Nanjing Medical UniversityNanjingChina Department of Oncology The First Affiliated HospitalSchool of MedicineShihezi UniversityShiheziChina Department of Oncology People’s Hospital of Xinjiang Uygur Autonomous RegionXinjiangChina Department of Medical Oncology t

Objective:real-world diagnostic and treatment data for pancreatic cancer in china are *** such,the present study investigated the clinical characteristics,diagnosis,and treatment of advanced pancreatic cancer(including locally advanced and metastatic disease)in the Hospital-based Advanced pancreatic Cancer Cohort in china of the china pancreas Data Center ***:A total of 5349 Chinese patients with advanced pancreatic cancer were identified from a *** entire course of real-world pancreatic cancer management was ***:The proportion of patients with advanced pancreatic cancer was higher among males than females(62.4%vs 37.6%,respectively).patients typically had a history of hypertension(30.8%),diabetes(21.6%),and cholangitis(20.2%).Abdominal pain(51.6%),abdominal distension(27.1%),jaundice(20.1%),and weight loss(16.3%)were the main symptoms observed in patients with advanced pancreatic cancer in this *** carbohydrate antigen(CA)19-9 is one of the most common tumor *** the present study,2562 patients underwent first-line *** median progression-free survival(pFS)for patients undergoing first-line therapy was 4.1 *** major options for first-line therapy included gemcitabine(GEM)plus S-1(GS/X)(23.4%),nab-paclitaxel plus GEM(AG)(18.1%),oxaliplatin,irinotecan,and leucovorin-modulated fluorouracil(FOLFIrINOX;11.9%),nab-paclitaxel plus S-1(AS)(8.9%),and GEM combined with oxaliplatin/cisplatin(GEMOX/Gp)(7.6%).The AS and GS/X regimens were associated with the highest pFS ***:This is the first study to report multicenter,real-world data regarding advanced pancreatic cancer in *** revealed that real-world treatment options differed from guideline recommendations,and pFS was shorter than that in previously reported *** intelligent follow-up systems and standardizing diagnosis and treatment of pancreatic cancer is recommended.

关键词： Advanced pancreatic cancer china pancreas Data Center Chinese First-line regimens real-world study