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Molecular Plant 2009年第3期2卷 430-441页

作者： Yong-Ch.n Li Jiang-Ping Ren Myeong-je Ch. Su-Mei Zh.u Yong-b.m Kim h.ng-Xiang Guo Josh.a h. Wong h.ng-b.n Niu h.un-Kyung Kim Susumu Morigasaki Peggy G. Lemaux Oscar L. Frick Jun Yin b.b.b. b.ch.nan National Engineering Research.Centre for Wh.at Henan Agricultural University Zhengzhou 450002 China Department of Plant and Microb.al b.ology 111 Koshland Hall University of California Berkeley CA 94720 USA Department of Pediatrics School of Medicine University of California San Francisco CA 94143 USA

Work with.cereals （b.rley and wh.at） and a legume （Medicago truncatula） h.s estab.ish.d th.oredoxin h.（Trx h. as a central regulatory protein of seeds. Trx h.acts b. reducing disulfide （S-S） groups of diverse seed proteins （storage proteins, enzymes, and enzyme inh.b.tors）, th.reb. facilitating germination. Early in vitro protein studies were complemented with.experiments in wh.ch.b.rley seeds with.Trx h.overexpressed in th. endosperm sh.wed accelerated germination and early or enh.nced expression of associated enzymes （α-amylase and pullulanase）. Th. current study extends th. transgenic work to wh.at. Two approach.s were followed to alter th. expression of Trx h.genes in th. endosperm：（1） a h.rdein promoter and its protein b.dy targeting sequence led to overexpression of Trx h., and （2） an antisense construct of Trx h. resulted in cytosolic underexpression of th.t gene （Arab.dopsis designation）. Underexpression of Trx h. led to effects opposite to th.se ob.erved for overexpression Trx h. in b.rley--retardation of germination and delayed or reduced expression of associated enzymes. Similar enzyme ch.nges were ob.erved in developing seeds. Th. wh.at lines with.underexpressed Trx sh.wed delayed preh.rvest sprouting wh.n grown in th. greenh.use or field with.ut a decrease in final yield. Wh.at with.overexpressed Trx h. sh.wed ch.nges commensurate with.earlier in vitro work： increased solub.lity of disulfide proteins and lower aUergenicity of th. gliadin fraction. Th. results are furth.r evidence th.t th. level of Trx h.in cereal endosperm determines fundamental properties as well as potential applications of th. seed.

关键词： metab.lic regulation molecular ph.siology seed b.ology preh.rvest sprouting seed germination th.oredoxin h wh.at allergenicity.

Mutations in precore and core promoter region of h.0} in patients with.h.patic failure

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h.patob.liary & Pancreatic Diseases International 2002年第1期1卷 63-67页

作者： Reinh.rd h. Dennin Jian-Er Wo Institute of Microb.ology Luebeck Medical College Germany Zh.jiang University Sch.ol of Medicine Hangzhou 310003China

Ob.0}ctive: To clarify th. association of h.patitis b.irus mutants in precore and core promoter regions inpatients with.h.patic failure and h.0}Ag ***: Precore and core promoter regions of 25h.0} isolates from th. patients with.h.patic failurewere analyzed b. polymerase ch.in reaction (PCR)direct sequencing ***: Precore G-to-A^(1896) mutants were identified in16 (64%) of th. 25 isolates. Th. 'h.t spot' mutationsat A-to-T^(1762) and G-to-A^(1764) were present togeth.r in19 (76%) of th. 25 isolates, wh.le C-to-T^(1653) and T-to-C^(1753) existed in a mutually exclusive manner andmore frequently in h.patic failure with.liver cirrh.sisgroup th.n in h.patic failure with.ch.onic h.patitisgroup (100% vs 50%). b.th.A^(1896) and T^(1762)-A^(1764)could b. found frequently in h.0}Ag-positive sub.0}cts(77.8% and 88.9%), wh.reas T^(1653)/C^(1753) was moreprevalent in anti-h.0}-positive sub.0}cts th.n inh.0}Ag-positive sub.0}cts (93.8% vs 33.3%).Conclusions: Th. wh.le frequency of mutations in pre-core and core promoter gene will b.come more fre-quent as h.0} infection is to b. persistent. Mutationto T^(1653)/C^(1753) may b. useful as a marker for h.paticfailure. It requires furth.r study wh.th.r th. mixedinfection of mutants and wilds will develop and affectth. condition of h.0}Ag in serum along th. progres-sion of liver disease.

关键词： mutations gene h.patitis b.virus

Controlling h.poxia-inducib.e factor-2α is critical for maintaining b.ne h.meostasis in mice

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b.ne Research 2019年第2期7卷 210-223页

作者： Sun Young Lee Ka h.on Park h.ung-Gu Yu Eunb.ul Kook Won-h.un Song Gyuseok Lee jeong-Tae Koh.h.ng-In Sh.n je-Yong Ch.i Yun h.un h.h.je-h.ang Ryu Department of Ph.rmacology and Dental Th.rapeutics School of DentistryChonnam National UniversityGwangju 61186Korea Research.Center for b.omineralization Disorders School of DentistryChonnam National UniversityGwangju 61186Korea Department of Oral Path.logy and Ih.0} School of DentistryKyungpook National UniversityDaegu 41940Korea Department of b.och.mistry and Cell b.ology BK21 Plus KNU Biomedical Convergence ProgramSkeletal Diseases Analysis CenterKorea Mouse Phenotyping Center (KMPC)School of MedicineKyungpook National UniversityDaegu 41944Korea Sch.ol of Life Sciences Gwangju Institute of Science and TechnologyGwangju 61005Korea

Path.logical b.ne loss is caused b. an imb.lance b.tween b.ne formation and *** b.ne microenvironments are h.poxic,and h.poxia-inducib.e factor (h.F) is known to play notab.e roles in b.ne ***,th. relevant functions of h.F-2α are not well ***,we h.ve sh.wn th.t h.F-2α deficiency in mice enh.nces b.ne mass th.ough.its effects on th. differentiation of osteob.asts and *** vitro analyses revealed th.t h.F-2α inh.b.ts osteob.ast differentiation b. targeting Twist2 and stimulates RANKL-induced osteoclastogenesis via regulation of *** addition,h.F-2α appears to contrib.te to th. crosstalk b.tween osteob.asts and osteoclasts b. directly targeting RANKL in osteoprogenitor *** performed with.osteob.ast- and osteoclast-specific conditional knockout mice supported a role of h.F-2α in th.s ***-2α deficiency alleviated ovariectomy-induced b.ne loss in mice,and specific inh.b.tion of h.F-2α with.ZINC04179524 significantly b.ocked RANKLmediated ***,our results suggest th.t h.F-2α functions as a catab.lic regulator in b.ne remodeling,wh.ch.is critical for th. maintenance of b.ne h.meostasis.

关键词： is RANKL its via

Wildland fires and moist deciduous forests of Ch.attisgarh. India: divergent component assessment

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Journal of Forestry Research 2014年第4期25卷 857-866页

作者： b.h.Kittur S.L.Swamy S.S.b.rgali Manoj Kumar Jh.riya Department of Silviculture & Agroforestry College of Forestry Kerala Agriculture University Vellanikara Thrissure Kerala India Department of Forestry College of Agriculture Indira Gandhi Krishi Vishwavidyalaya Raipur (C.G.) 492006 India Department of b.tany DSB Campus Kumaun University Nainital–263001 (Uttarakhand) India

We studied moist deciduous forests of Ch.attisgarh. India （1） to assess th. effect of four levels of h.storic wildland fire frequency （h.gh. medium, low, and no-fire） on regeneration of seedlings in fire affected areas during pre and post-fire seasons, （2） to evaluate vegetation struc- ture and diversity b. layer in th. four fire frequency zones, （3） to evalu- ate th. impact of fire frequency on th. structure of economically impor- tant tree species of th. region, and （4） to quantify fuel loads b. fire fre- quency level. We classified fire-affected areas into h.gh. medium, low, and no-fire frequency classes b.sed on government records. Tree species were unevenly distrib.ted across fire frequency categories. Sh.ub.density was maximum in zones of h.gh.fire frequency and minimum in low- frequency and no-fire zones. Lower tree density after fires indicated th.t regeneration of seedlings was reduced b. fire. Th. population structure in th. h.gh.frequency zone was comprised of seedlings of size class （A） and saplings of size class （b., represented b. Diospyros melanoxylon, Dalb.rgia sissoo, Sh.rea rob.sta and Tectona grandis. Younger and older trees were more ab.ndant for Tectona grandis and Dalb.rgia sis- soo after fire, wh.reas intermediate-aged trees were more ab.ndant pre- fire, indicating th.t th. latter age-class was th.nned b. th. catastroph.c effect of fire. Th. major contrib.ting components of fuel load included duff litter and small woody b.anch.s and twigs on th. forest floor. Total fuel load on th. forest floor ranged from 2.2 to 3.38 Mg/h.. Th. netch.nge in fuel load was positive in h.gh. and medium-frequency fire zones and negative under low- and no-fire zones. Repeated fires, h.w- ever, slowly reduced stand stab.lity. An ecological approach.is needed for fire management to restore th. no-fire spatial and temporal structure of moist deciduous forests, th.ir species composition and fuel loads. Th. management approach.sh.uld incorporate participatory forest ma

关键词： forest fire diversity fuel load vegetation structure

Room-temperature sputtered electrocatalyst WSe2 nanomaterials for h.drogen evolution reaction

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Journal of Energy Ch.mistry 2020年第8期29卷 107-111,I0004页

作者： Jae h.eon Nam Myeong je Jang h.e Yeon Jang Woojin Park Xiaolei Wang Sung Mook Ch.i b.ungjin Ch. Department of Advanced Material Engineering Chungbuk National UniversityChungdae-ro 1Seowon-GuCheongjuChungbuk 28644Republic of Korea Surface Tech.ology Department Korea Institute of Materials Science797 ChangwonDaeroSungsan-guChangwon 51508Republic of Korea Advanced Materials Engineering Korea University of Science and Technology(UST)217 Gajeong-roYuseong-guDaejeon 34113Republic of Korea Department of Ch.mical and Materials Engineering University of Alberta9211116 Street NWEdmontonAlberta T6C 1H9Canada

Th. low-temperature ph.sical vapor deposition process of atomically th.n two-dimensional transition metal dich.lcogenide(2D TMD) h.s b.en gaining attention owing to th. cost-effective production of diverse electroch.mical catalysts for h.drogen evolution reaction(h.R) applications. We, h.rein, propose a simple route toward th. cost-effective ph.sical vapor deposition process of 2D WSe2 layered nanofilms as h.R electroch.mical catalysts using RF magnetron sputtering at room temperature(b. controlling th. variab.e sputtering parameters, such.as RF power and deposition time, th. loading amount and electroch.mical surface area(ECSA) of WSe2 films deposited on carb.n paper can b. carefully determined. Th. surface of th. sputtered WSe2 films are partially oxidized, wh.ch.may cause sph.rical-sh.ped particles. Regardless of th. loading amount of WSe2, Tafel slopes of WSe2 electrodes in th. h.R test are narrowly distrib.ted to b. ~120–138 mV dec-1, wh.ch.indicates th. excellent reproducib.lity of intrinsic catalytic activity. b. considering th. trade-off b.tween th. loading amount and ECSA, th. b.st h.R performance is clearly ob.erved in th. 200 W-15 min sample with.an overpotential of 220 mV at a current density of 10 mA cm-2. Such.a simple sputtering meth.d at low temperature can b. easily expanded to oth.r 2D TMD electroch.mical catalysts, promising potentially practical electrocatalysts.

关键词： Two dimensional nanomaterials Sputtering WSe2 nanofilm Electrocatalyst h.drogen evolution reaction

幽门螺杆菌cagⅡ对胃上皮细胞IL-8基因转录的影响及机制

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中华消化杂志 2000年第4期20卷 230-233页

作者：李淑德许国铭李兆申 Crab.ree je Neelam b 第二军医大学长海医院上海市200433 英国LeedsSt.James医院分子医学部

目的　探讨h.cagⅡ对胃上皮细胞IL 8基因转录的影响及信号传导机制。方法　构建cagⅡ基因位点缺失h.突变株及带有IL 8报告基因的人胃癌细胞系L5F11,用液体闪烁计数仪测定荧光素酶 (IL 8转录 )活性 ,用ELISA法测定IL 8蛋白浓度。结果　... 详细信息

目的　探讨h.cagⅡ对胃上皮细胞IL 8基因转录的影响及信号传导机制。方法　构建cagⅡ基因位点缺失h.突变株及带有IL 8报告基因的人胃癌细胞系L5F11,用液体闪烁计数仪测定荧光素酶 (IL 8转录 )活性 ,用ELISA法测定IL 8蛋白浓度。结果　所有h.突变株诱导荧光素酶活性与IL 8蛋白浓度较亲代菌株 2 6 6 95均降低 [(0 .13± 0 .0 1)× 10 6cpm比 (0 .5 9± 0 .0 5 )× 10 6cpm (P h.rb.mycinA不仅抑制h.诱导的荧光素酶活性 [(0 .71± 0 .18)× 10 6cpm比 (1.5 1±0 .2 3)× 10 6cpm ,(P h.rb.mycinA对TNFα诱导的荧光素酶活性及IL 8蛋白表达均无影响 (P均 >0 .0 5 ) ;PKA抑制剂h.抑制TNFα诱导的荧光素酶活性 [(0 .74± 0 .16 )× 10 6cpm比 (2 .6 2± 0 .2 6 )× 10 6cpm ,(P h.诱导的荧光素酶活性无影响 (P >0 .0 5 )。结论　h.cagⅡ中的多个基因能够调节胃上皮细胞IL 8基因转录。

关键词：幽门螺杆菌 cagⅡ 胃上皮细胞白细胞介素-8

Sequential disruption of ALV h.st receptor genes reveals no sh.ring of receptors b.tween ALV sub.roups A, b. and J

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Journal of Animal Science and b.otech.ology 2019年第2期10卷 410-423页

作者： h.ng Jo Lee Kyung je Park Kyung Youn Lee Yongxiu Yao Venugopal Nair Jae Yong h.n Sciences and Research Institute of Agriculture and Life SciencesSeoul National UniversitySeoul 08826Korea Th. Pirb.igh. Institute WokingSurrey GU24 0NFUK.

b.ckground: Previously, we sh.wed th.t targeted disruption of viral receptor genes in avian leukosis virus(ALV)sub.roups using clustered regularly interspaced sh.rt palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9))-b.sed genome editing confers resistance to ALV sub.roups b.and J. h.re, we used th. same strategy to target th. receptor expressed b. ALV sub.roup A(TVA) and generate ch.cken cells resistant to infection b. th.s ***: CRISPR/Cas9-b.sed disruption of exon 2 with.n th. tva gene of DF-1 fib.ob.asts conferred resistance to infection b. ALV sub.roup A regardless of wh.th.r framesh.ft mutations were introduced during editing. Conversely,overexpression of th. wild-type TVA receptor(wtTVA) b. tva-modified DF-1 clones restored susceptib.lity to ALV sub.roup A. Th. results confirm th.t exon 2, wh.ch.contains th. low-density lipoprotein receptor class A domain of TVA, is critical for virus entry. Furth.rmore, we sequentially modified DF-1 cells b. editing th. tva, tvb. and Na^+/h.+ exch.nge 1(ch.h.1) genes, wh.ch.are th. specific receptors for ALV sub.roups A, b. and J, ***: Simultaneous editing of multiple receptors to b.ock infection b. different sub.roups of ALV confirmed th.t ALV sub.roups A, b. and J do not sh.re h.st receptors. Th.s strategy could b. used to generate cells resistant to multiple viral path.gens th.t use distinct receptors for cell entry.

关键词： Avian leukosis virus CRISPR/Cas9 Genome editing h.st receptor TVA

Influence of deh.drating agents on th. oxidative carb.nylation of meth.nol for dimeth.l carb.nate synth.sis over a Cu/Y-zeolite catalyst

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Ch.nese Journal of Ch.mical Engineering 2018年第5期26卷 1059-1063页

作者： Dong-h. Lee Jiin You je-Min Woo Jung Yoon Seo Young Ch.ol Park Jong-Seop Lee h.unuk Kim Jong-h. Moon Seung b.n Park Greenh.use Gas Lab.ratory Korea Institute of Energy Research Department of Ch.mical and b.omolecular Engineering Korea Advanced Institute of Science and Technology Energy Material Lab.ratory Korea Institute of Energy Research

Th. influence of th. deh.dration b. metal oxides on th. synth.sis of dimeth.l carb.nate （DMC） via oxidative carb.nylation of meth.nol was studied. A Cu/Y-zeolite catalyst was prepared b. th. ion exch.nge meth.d from CuCl2.2h.O and th. commercial Nh.-form of th. Y type zeolite, Th. catalyst was ch.racterized b. X-ray fluorescence （XRF）, N2 adsorption （b.T meth.d）, X-ray diffraction （XRD）, and temperature-programmed de- sorption of ammonia （Nh.-TPD） to evaluate its Cu and Cl content, surface area, structure, and acidity. Reaction tests were carried out using an autoclave （b.tch.reactor） for 18 h.at 403 K and 5.5 MPa （2Ch.Oh.＋ 1/2O2 ＋ CO （Ch.O）2CO ＋ h.O）. Th. influence of various deh.drating agents （ZnO, MgO, and CaO） was examined with.th. aim of increasing th. meth.nol conversion （XMeOh. MeOh.conversion）. Th. MeOh.conversion increased upon addition of metal oxides in th. order CaO 〉〉 MgO 〉 ZnO, with.th. DMC selectivity （SDMC） following th. order MgO 〉 CaO 〉 ZnO. Th. catalysts and deh.drating agents were ch.racterized b.fore and after th. oxidative carb.nylation of meth.nol b. th.rmogravimetric and differential th.rmogravimetric （TG/DTG）, and XRD to con- firm th.t th. deh.dration reaction occurred via th. metal oxide （MO ＋ h.O →M（Oh.2）. Th. MeOh.conversion increased from 8.7% to 14.6% and DMC selectivity increased from 39.0% to 53.1%, wh.n using th. deh.drating azent CaO.

关键词： Carb.n monoxide Zeolite Catalyst Dimeth.l carb.nate Oxidative carb.nylation Deh.drating agent

Th. comparison of auditory b.h.vioral and evoked potential responses (steadystate and cortical) in sub.0}cts with.occupational noise-induced h.aring loss

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Journal of Otology 2021年第4期16卷 242-251页

作者： P.h.DeJonckere J.Leb.cq Federal Agency for Occupational Risks Avenue de l’Astronomie1B-1210BruxellesBrusselsBelgium Institute of Neurosciences University of LouvainPasteurAvenue Mounier53B-1200BruxellesBrusselsBelgium

Ob.0}ctive: To define difference scores b.tween PTA, ASSR and CERA th.esh.lds in sub.0}cts with.occupational ***: 44 sub.0}cts undergoing a medico-legal expert assessment for occupational NIh. and fulfillingcriteria of reliab.lity were considered. Assessment included: PTA, 40 h. b.naural multiple ASSR and CERA(1-2-3 kh.).Results: Th. respective average difference scores (ASSR - PTA) for 1, 2 and 3 kh. are 13.01 (SD 10.19) db.12.72 (SD 8.81) db.and 10.38 (SD 8.19) db. Th. average (CERA - ASSR) difference scores are 1.25 (SD 14.63)db.for 1 kh. (NS), 2.73 (SD 13.03) db.for 2 kh. (NS) and 4.51 (SD 12.18) db.for 3 kh.. Th. correlationb.tween PTA and ASSR (0.82) is significantly stronger th.n th.t b.tween PTA and CERA (0.71). In a givensub.0}ct, PTA th.esh.lds are nearly always lower (i.e., b.tter) th.n ASSR th.esh.lds, wh.tever th. frequency (1-2-3 kh.) and th. side (righ. e left). A significant negative correlation is found b.tween th.difference score (ASSR e PTA) and th. degree of h.aring ***: ASSR outperforms CERA in a medicolegal context, alth.ugh.overestimating th. b.h.vioralth.esh.lds b. 10e13 db.

关键词： CERA ASSR NIh. h.aring th.esh.lds Occupational