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Progress in Natural Science:Materials International 2007年第2期17卷 150-155页

作者： tien po Beijing 100080 China Chinese Academy of Sciences Molecular Virology Research Center Institute of Microbiology

Membrane fusion is a key step in enveloped virus entry. Highly conserved heptad repeat regions (HR1 and HR2) of Newcastle disease virus (NDV) fusion protein (F) are critical functional domains for viral membrane fusion. They display different conformations in the membrane fusion states and are viewed as candidate targets for neutralizing antibody responses. We previously reported that an analog of heptad repeat peptides HR2-HR1-HR2(HR212) and HR2 could inhibit NDV induced cell-cell membrane fusion. Here, we show that HR212 can induce the production of highly potent antibody in immunized rabbits, which could recognize full length peptides of both HR1 and HR2, and inhibit NDV hemagglutination and NDV entry. These suggest that either HR212 or its antibody could be an inhibitor of virus-induced cell-cell membrane fusion.

关键词： NDV heptad repeat domains immune response inhibition of viral entry.

Hepatitis B virus X protein suppresses virus-triggered IRF3 activation and IFN-β induction by disrupting the VISA-associated complex

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Cellular & Molecular Immunology 2010年第5期7卷 341-348页

作者： Xianmiao Wang Ying Li Aiping Mao Chao Li Yongkui Li po tien College of Life Sciences Modern Virology CenterWuhan UniversityWuhan 430072China

Viral RNAs produced during viral infection are recognized by the cytoplasmic RNA helicases retinoic acid-inducible gene-I(RIG-I)and melanoma differentiation-associated gene 5(MDA5).A central adapter protein downstream of RIG-I and MDA5 is the mitochondrial membrane protein virus-induced signaling adaptor(VISA),which mediates the induction of type I interferons(IFNs)through the activation of transcription factors such as nuclear factor-kappaB(NF-kB)and IFN-regulatory factor-3(IRF3).Here we found that hepatitis B virus(HBV)-encoded X protein(HBx)acts as an inhibitor of virus-triggered IRF3 activation and IFN-b *** and plaque assays indicate that HBx inhibits signaling by components upstream but not downstream of *** experiments indicate that HBx interacts with VISA and disrupts the association of VISA with its upstream and downstream *** findings suggest that HBx acts as a suppressor of virus-triggered induction of type I IFNs,which explains the observation that HBV causes transient and chronic infection in hepatocytes but fails to activate the pattern recognition receptor-mediated IFN induction pathways.

关键词： HBx innate immunity signal transduction type I interferons VISA

Cloning of M and NP Gene of H5N1 Avian Influenza Virus and Immune Efficacy of their DNA Vaccines

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中国病毒学 2007年第1期22卷 46-52页

作者： po tien Modern Virology Center State Key Laboratory of VirologyCollege of Life SciencesWuhan UniversityWuhan 430072China

H5N1 鸟的流行性感冒病毒(A/chicken/Hubei/489/2004 ) 的 M 和 NP 基因被 RT-PCR 从病毒的 RNA 放大，并且分别地克隆向量进 pMD18-T。包含 M 基因(pHM6-m ) 或 NP 基因(pHM6-np ) 的表示 plasmid 然后被把 M 或 NP 基因插入到 pHM6 ... 详细信息

H5N1 鸟的流行性感冒病毒(A/chicken/Hubei/489/2004 ) 的 M 和 NP 基因被 RT-PCR 从病毒的 RNA 放大，并且分别地克隆向量进 pMD18-T。包含 M 基因(pHM6-m ) 或 NP 基因(pHM6-np ) 的表示 plasmid 然后被把 M 或 NP 基因插入到 pHM6 优核质表示向量构造；构造 plasmid 然后被定序。32 只 BALB/c 老鼠(6-week-old ) 在随机被划分成四个组。三组 BALB/c 老鼠被接种一次有 plasmid pHM6-m， plasmid pHM6-np 的 30 渭 g 或 plasmid pHM6-m (15 渭 g ) 和 pHM6-np (15 渭 g ) 的混合的任何一个 30 渭 g 的肌内的线路分别地。老鼠的一个另外的组作为控制与 100 渭 l PBS 被注射。二个星期以后，所有老鼠与相应 H5N1 鸟的流行性感冒病毒被质问，并且在下列 12 天内观察了。在 pHM6-m 组， pHM6-np 组和混合 plasmids 组的老鼠的幸存率分别地是 62.5% ， 25.0% 和 50.0% 。结果证明有效保护能被 pHM6-m 或 pHM6-np 提供，但是 pHM6-m 比 pHM6-np 提供了更好保护的效果。关键词 H5N1 流行性感冒病毒 - M 基因 - NP 基因 - 克隆 - DNA 疫苗的 CLC 数字 S852.65 基础条款：国家基本科学才能训练资助(NSFC J0630648 )

关键词： H5N1 influenza virus M gene NP gene Cloning DNA vaccine

Mitochondria Redistribution in Enterovirus A71 Infected Cells and Its Effect on Virus Replication

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Virologica Sinica 2019年第4期34卷 397-411页

作者： Yang Yang Haolong Cong Ning Du Xiaodong Han Lei Song Wenliang Zhang Chunrui Li po tien Center for Molecular Virology CAS Key Laboratory of Pathogenic Microbiology and ImmunologyInstitute of MicrobiologyChinese Academy of SciencesBeijing 100101China University of the Chinese Academy of Sciences Beijing 100101China Beijing Institutes of Life Science Chinese Academy of SciencesBeijing 100101China College of Life Sciences Inner Mongolia Agriculture UniversityHohhot 010018China

Enterovirus A71(EV-A71)is one of the main causative agents of hand,foot and mouth disease(HFMD)and it also causes severe neurologic complications in infected *** interactions between some viruses and the host mitochondria are crucial for virus replication and *** this study,it was observed that EV-A71 infection resulted in a perinuclear redistribution of the *** mitochondria rearrangement was found to require the microtubule network,the dynein complex and a low cytosolic calcium ***,the EV-A71 non-structural protein 2BC was identified as the viral protein capable of inducing mitochondria *** protein was found localized on mitochondria and interacted with the mitochondrial Rho GTPase 1(RHOT1)that is a key protein required for attachment between the mitochondria and the motor proteins,which are responsible for the control of mitochondria ***,suppressing mitochondria clustering by treating cells with nocodazole,EHNA,thapsigargin or A23187 consistently inhibited EV?A71 replication,indicating that mitochondria recruitment played a crucial role in the EV-A71 life *** study identified a novel function of the EV-A71 2BC protein and provided a potential model for the regulation of mitochondrial motility in EV-A71 infection.

关键词： Enterovirus A71(EV-A71) Mitochondria Microtubule network Calcium concentration Mitochondrial Rho GTPase 1(RHOT1)

Synthesis and antiviral activities of amino acid derivatives of Gossypol

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Synthesis and antiviral activities of amino acid derivatives...

Specific inhibition of HIV-1 replication by hCycT1 shRNA without inducing apoptosis

2011年全国药物化学学术会议——药物的源头创新

作者： po tien The College of Life Sciences State Key Laboratory of VirologyModern Virology Research CenterWuhan University

Twenty-one amino acid derivatives of gossypol have been synthesized and screened in vitro for their anti-HIV-1 activity and anti-HN activity *** the study,we found that some amino acids,replacing the aldehyde groups of gossypol,not only lower the cytotoxicity of gossypol, but also enhanced the activities of gossypol against HIV-1 and *** absence of the COONa

关键词： gossypol amino acid derivatives HIV-1 avian influenza virus Molecular modeling

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Specific inhibition of HIV-1 replication by hCycT1 shRNA wit...

FN-λ： A new spotlight in innate immunity against influenza virus infection

第二届中国青年学者微生物遗传学学术研讨会

作者： po tien The Modern Virology Centre and State Key Laboratory of Virology College of Life Sciences Wuhan University Wuhan 430072P.R.China

RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) triggers the silencing of gene expression in a sequence-specific manner. In mammalian cells, RNAi can be triggered by 21-nucleotide small interfering RNA (siRNA) duplexes. RNAi provides a methodology for addressing the role of cellular and viral regulatory factors in the virus life cycle, and the induction of an

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Protein & Cell 2018年第10期9卷 832-837页

作者： Yeping Sun Jingwen Jiang po tien Wenjun Liu Jing Li CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of SciencesBeijing 100101 China

Influenza virus is a long-lasting and severe threat to human health. Seasonal flu epidemics, which are caused by the co- circulating influenza A viruses （IAVs） and influenza B viruses （IBVs）, occur annually and lead to tens of millions of respiratory illnesses and up to half a million human deaths worldwide each year （Ginsberg et al., 2009）. Influenza pandemics are more devastating. The 2009 swine-originated H1N1 virus, which caused the latest influenza pandemic, spread from Mexico and U.S.

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Stability Engineering and Crystal Structure of NK Cell Activating Receptor Immunoglobulin-Like Transcript 1 (ILT1/LIR-7):A 3D Domain Swapped Dimer

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Stability Engineering and Crystal Structure of NK Cell Activ...

miR373和miR542-5p调控肠道病毒71型在人横纹肌肉瘤细胞中的复制

2008年全国生物化学与分子生物学学术大会

作者： po tien George F.Gao Center for Molecular Virology Institute of MicrobiologyChinese Academy of Sciences (CAS) China-Japan Joint Laboratory of Molecular Immunology and Molecular Microbiology

Natural Killer (NK) cells are involved in the recognition and elimination of the virus-infected and malignant cells through the equilibrium of signals produced by inhibitory and activating receptors,apart from their o...

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生物工程学报 2014年第6期30卷 943-953页

作者：杨倬田波中国疾病预防控制中心营养与食品安全所北京100050 中国科学院微生物研究所病原微生物与免疫学重点实验室北京100101

研究发现microRNAs(miRNAs)可以参与调控病毒在宿主细胞内感染和复制的过程。为了揭示miRNAs是否参与肠道病毒71型(Enterovirus 71,EV71)的感染与复制,研究了miRNAs对EV71病毒在宿主细胞内复制的影响。构建miRNAs靶基因筛选系统,在双荧... 详细信息

研究发现microRNAs(miRNAs)可以参与调控病毒在宿主细胞内感染和复制的过程。为了揭示miRNAs是否参与肠道病毒71型(Enterovirus 71,EV71)的感染与复制,研究了miRNAs对EV71病毒在宿主细胞内复制的影响。构建miRNAs靶基因筛选系统,在双荧光素酶报告体系的pMIR载体插入病毒基因,如果插入的基因序列能被细胞内的miRNAs靶向调控,报告基因的表达将发生变化。实验发现EV71病毒5′-UTR基因可能是miRNAs的作用靶标。随后利用miRNAs在线分析软件预测并验证可能作用于5′-UTR基因片段的miRNAs。为了研究miRNAs分子对5′-UTR基因的调控作用是否可以体现在EV71病毒的复制过程中,在人横纹肌肉瘤(Rhabdomyosarcoma,RD)细胞中转染miRNAs mimics,利用Western blotting和real-time PCR实验检验EV71病毒的复制和表达情况。实验结果表明,miR373和miR542-5p可以通过作用于EV71病毒5′-UTR基因从而抑制病毒在RD细胞中的复制和表达。细胞内miR373和miR542-5p可以调控EV71在宿主细胞中的复制过程。研究EV71病毒与宿主miRNAs的相互作用机制为进一步阐明EV71病毒感染与复制机理奠定了基础。

关键词： miRNAs 肠道病毒71型人横纹肌肉瘤细胞病毒复制

热休克蛋白-多肽复合物在肿瘤和传染性疾病免疫中的作用

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生物工程学报 2000年第4期16卷 425-428页

作者：孟颂东高福田波中国科学院微生物研究所北京100080 Harvard University Boston Massachusetts

热休克蛋白家族中的许多成员如 gp96、HSP90、HSP70等具有排斥和治疗肿瘤及传染性疾病的免疫原性 ,进一步研究发现热休克蛋白作为分子伴侣可结合细胞中的肽库 ,它本身没有抗原性 ,抗原性由结合的短肽所决定。热休克蛋白将结合的短肽呈递... 详细信息

热休克蛋白家族中的许多成员如 gp96、HSP90、HSP70等具有排斥和治疗肿瘤及传染性疾病的免疫原性 ,进一步研究发现热休克蛋白作为分子伴侣可结合细胞中的肽库 ,它本身没有抗原性 ,抗原性由结合的短肽所决定。热休克蛋白将结合的短肽呈递给I类MHC分子 ,进而激活特异性CTL和记忆性T细胞 ,引发机体细胞免疫反应。据最新发现gp96还可能有与MHC一样的功能 ,可直接将结合的多肽抗原呈递给T细胞。近年来对哺乳动物的二种主要热休克蛋白 gp96和HSP70的免疫机制和作为治疗性疫苗的优越性进行了详细研究 ,这为乙型肝炎和乙肝继发性肝癌的免疫治疗提供了新思路。

关键词： HSPS 肿瘤免疫 CTL 抗原呈递肽疫苗传染病免疫