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基于动态血糖监测结果评价门诊教育对2型糖尿病患者血糖谱的影响研究

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中国全科医学 2024年第21期27卷 2572-2577页

作者：周潇周云婷孔小岑刘晓梅袁璐荆亭王蔚萍李惠琴南京医科大学附属南京医院南京市第一医院内分泌科江苏省南京市210029

背景动态血糖监测(CGM)在门诊患者治疗中应用较少,基于CGM进行糖尿病教育,改变不良的生活、饮食习惯对血糖谱的影响尚不清楚。目的基于回顾性CGM结果,对使用口服降糖药的门诊2型糖尿病(T2DM)患者进行饮食、运动等门诊教育,评价其对患... 详细信息

背景动态血糖监测(CGM)在门诊患者治疗中应用较少,基于CGM进行糖尿病教育,改变不良的生活、饮食习惯对血糖谱的影响尚不清楚。目的基于回顾性CGM结果,对使用口服降糖药的门诊2型糖尿病(T2DM)患者进行饮食、运动等门诊教育,评价其对患者血糖谱的影响。方法选取2021于南京市第一医院内分泌科门诊就诊使用口服降糖药的T2DM患者88例,其中男60例、女28例,给予CGM。入组患者给予门诊教育,即第1~3天维持原降糖治疗方案及生活习惯;第4天下载并分析CGM数据,结合患者饮食、运动等门诊教育,第6天取下CGM传感器和记录器,下载数据。比较第2天和第5天动态血糖数据[24 h平均血糖(MBG),平均血糖波动幅度(MAGE),葡萄糖目标范围内时间(TIR)]。结果基于CGM结果,给予门诊教育后(第5天)T2DM患者的MBG由(8.34±1.97)mmol/L下降到(7.85±1.65)mmol/L,TIR由(78.21±24.64)%上升到(84.28±21.87)%,MAGE由(4.53±2.25)mmol/L下降到(3.80±1.80)mmol/L(P<0.05)。按糖尿病病程、年龄进行分层分析:病程<10年组(62例)和病程≥10年组(26例)干预后MBG均较干预前降低,干预后病程<10年组标准差(SD)、MAGE、TAR较干预前降低,TIR较干预前升高(P<0.05);按年龄分为<65岁组(55例)和≥65岁组(33例),两组干预后MBG、TAR较干预前降低,TIR较干预前升高,干预后<65岁组SD、MAGE较干预前降低(P<0.05)。结论基于CGM结果,对使用口服降糖药物的门诊T2DM患者进行饮食、运动等门诊教育,可以有效改善患者MBG、提高TIR水平,改善血糖变异度;<65岁、糖尿病病程<10年的患者获益更大。

关键词：血糖自我监测动态血糖监测糖尿病,2型健康教育血糖谱目标范围内时间

Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection

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Chinese Journal of Natural Medicines 2019年第9期17卷 650-662页

作者： GENG Zi-Kai LI Ya-Qun CUI Qing-Hua DU Rui-Kun tian jing-Zhen School of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China. Collaborative Innovation Center for Antiviral Traditional Chinese Medicine Jinan 250355China

Ge Gen Decoction(GGD),a Traditional Chinese Medicine prescription,is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus(IAV)***,the underlying mechanism of GGD against IAV infection remains *** this study,we found that GGD had moderate anti-IAV activity in *** was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization sta *** vivo,GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate,lung index,and pulmonary hist opathological changes in H1N1-infected *** observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the *** treatment decreased the expression of TNF-αand improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected ***,the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD *** results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.

关键词： Ge Gen Decoction Traditional Chinese Medicine(TCM) H1N1 Antiviral Immune regulation

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乌鳢三种性腺组织的转录组比较分析

农业生物技术学报 2024年第2期32卷 379-395页

作者：黄苏静吴玉霞夏威威张啸天赵建李凯彬罗青刘海洋费树站陈昆慈欧密中国水产科学研究院珠江水产研究所/农业农村部热带亚热带水产种质资源利用与养殖重点实验室上海海洋大学水产与生命学院

乌鳢(Channa argus)是我国重要的淡水经济鱼类，具有显著的性别二态性。为了研究乌鳢性别分化和性腺发育的分子机制，挖掘精、卵巢差异表达的功能基因和信号通路，本研究采用Illumina HiSeq 2000测序平台对6月龄乌鳢XX雌鱼(XX-F)、XY雄... 详细信息

乌鳢(Channa argus)是我国重要的淡水经济鱼类，具有显著的性别二态性。为了研究乌鳢性别分化和性腺发育的分子机制，挖掘精、卵巢差异表达的功能基因和信号通路，本研究采用Illumina HiSeq 2000测序平台对6月龄乌鳢XX雌鱼(XX-F)、XY雄鱼(XY-M)和YY超雄鱼(YY-M)的性腺组织进行了转录组测序(RNA-seq)分析。性腺组织两两样品的表达量相关性分析显示，XY-M精巢和YY-M精巢具有相似的表达模式，与XX-F卵巢表达模式相差甚远，与性腺的组织学结果一致。三种性腺组织的基因差异表达分析显示，XY-M vs XX-F中有12 317个差异表达基因(differentially expressed genes, DEGs)，其中5 427个上调、6 890个下调；XY-M vs YY-M中有212个DEGs，其中59个上调、153个下调；YY-M vs XX-F中有11 282个DEGs，其中5 038个上调、6 244个下调。利用qRT-PCR对性别分化和性腺发育相关的DEGs进行验证，发现抗缪勒管激素2型受体(anti-müllerian hormone receptor type 2, Amhr2)、抗缪勒管激素(anti-müllerian hormone, Amh)、double sex和mab3相关转录因子1(double sex and mab3-related transcription factor1,Dmrt1)、性腺体细胞衍生因子(gonadal somatic cell-derived factor, Gsdf)等10个基因在XY-M和YY-M精巢中高表达，骨形态发生蛋白15(bone morphogenetic protein 15, Bmp15)、性腺型芳香化酶基因Cyp19a1a(cytochrome P450 family 19 subfamily A member 1a)、叉头框转录因子2(forkhead transcription factor gene 2, Foxl2)等9个基因在XX-F卵巢中高表达。qRT-PCR验证结果与转录组分析结果一致，说明Amhr2、Amh、Dmrt1和Gsdf等基因与乌鳢精巢发育调控相关，Bmp15、Cyp19a1a和Foxl2等基因与乌鳢卵巢发育调控相关。另外，对DEGs进行KEGG代谢通路富集分析，发现较多的DEGs富集在性别分化和代谢相关通路，如丝裂原活化蛋白激酶(mitogen-activated protein kinase, MAPK)、无翅型MMTV整合位点家族成员(wingless-type MMTV integration site family, Wnt)以及转化生长因子-β(transforming growth factor-β, TGF-β)信号通路等。本研究筛选出了大量乌鳢性别分化和性腺发育相关的候选基因和信号通路，为后续深入研究乌鳢的性别分化和性腺发育的分子机制及性别相关基因的功能提供了基础资料。

关键词：乌鳢转录组差异表达基因(DEG) 性别分化性腺发育

Genome-wide association studies reveal the genetic basis of amino acid content variation in tea plants

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Journal of Integrative Agriculture 2023年第11期22卷 3364-3379页

作者： GUO Ya-fei LI Dai-li QIU Hai-ji ZHANG xiao-liang LIU Lin ZHAO jing-jing JIANG De-yuan National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhan 430070P.R.China

Tea is one of the most popular non-alcoholic beverages in the world,and free amino acids,especially theanine,make a major contribution to the umami taste of ***,the genetic basis of the variation in amino acid content in tea plants remains largely ***,we measured the free amino acid content in fresh leaves of 174 tea accessions over two years using a targeted metabolomics approach and obtained genotype data via RNA ***-wide association studies were conducted to investigate loci affecting the content of free amino acids.A total of 69 quantitative trait loci(–log10(P-value)>5)were *** annotation revealed that branched-chain amino acid aminotransferase,glutamine synthetase,nitrate transporter,and glutamate decarboxylase might be important for amino acid *** significant loci,glutamine synthetase(Glu1,P=3.71×10^(−4);Arg1,P=4.61×10^(−5))and branched-chain amino acid aminotransferase(Val1,P=4.67×10^(−5);I_Leu1,P=3.56×10^(−6)),were identified,*** on the genotyping result,two alleles of CsGS(CsGS-L and CsGS-H)and CsBCAT(CsBCAT-L and CsBCAT-H)were selected to perform function *** of CsGS-L and CsGS-H enhanced the contents of glutamate and arginine in transgenic plants,and overexpression of CsBCAT-L and CsBCAT-H promoted the accumulation of valine,isoleucine and *** activity assay uncovered that SNP1054 is important for CsGS catalyzing glutamate into ***,CsGS-L and CsGS-H differentially regulated the accumulation of glutamine,and CsBCAT-L and CsBCAT-H differentially regulated the accumulation of branched-chain amino *** summary,the findings in our study would provide new insights into the genetic basis of amino acids contents variation in tea plants and facilitate the identification of elite genes to enhance amino acids content.

关键词： Camellia sinensis amino acids genetic variation association studies genotype analysis functional verification

Single-seed sowing increased pod yield at a reduced seeding rate by improving root physiological state of Arachis hypogaea

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Journal of Integrative Agriculture 2020年第4期19卷 1019-1032页

作者： LIANG xiao-yan GUO Feng FENG Ye ZHANG Jia-lei YANG Sha MENG jing-jing LI Xin-guo WAN Shu-bo Shandong Institute of Sericulture Yantai 264002P.R.China Biotechnology Research Center Shandong Academy of Agricultural SciencesJinan 250100P.R.China Shandong Provincial Key Laboratory of Crop Genetic Improvement Ecology and PhysiologyJinan 250100P.R.China Agriculture Bureau of Yiyuan Zibo 256100P.R.China Shandong Academy of Agricultural Sciences Jinan 250100P.R.China

Double-seed sowing(two seeds per hole)is the dominant pattern of peanut sowing in China,but within-hole plant competition usually limits their growth and yield ***,the traditional double-seed sowing method does not facilitate mechanization during *** objective of this study was to determine if single-seed sowing at a proper seeding rate yielded better than traditional double-seed sowing pattern and the differences of physiological metabolism of roots.A field experiment was conducted in two consecutive years to compare pod yields of single-seed sowing at 180000(S180),225000(S225),and 270000 seeds ha^-1(S270)with that of double-seed sowing at 270000 seeds ha^-1(D270)using a completely randomized block design with four *** the root bleeding sap rate,nutrient content,and the main hormone contents in root bleeding sap were also comparatively *** the pod yields of single-seed sowing at the three densities were higher than that of traditional double-seed sowing(D270),S225 yielded better than the other two single-seed sowing treatments(S180 and S270).The increased pod yield in single-seed sowing at 225000 seeds ha^-1 was mainly due to the higher pod dry weight per plant and harvest *** improved pod dry weight and shoot growth had closely relationship with the enhanced root physiological traits such as the increased root bleeding sap rate,content of free amino acids,soluble sugars,K^+,Mg^2+,Zn^2+,and Ca^2+of the individual plant *** improved activity of root reductive,nitrate reductase(NR)and ATPase and higher zeatin and zeatin riboside(Z+ZR)content of root bleeding sap were alsocrucial to the pod and shoot growth of ***-seed sowing at a moderate seeding rate(S225)is a potential practice to increase pod yield and to save seed cost.

关键词： peanut sowing pattern seeding rate root traits yield

Effects of miR-200c on the migration and invasion abilities of human prostate cancer Du145 cells and the corresponding mechanism

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Frontiers of Medicine 2014年第4期8卷 456-463页

作者： Runlin Shi Haibing xiao Tao Yang Lei Chang Yuanfeng tian Bolin Wu Hua Xu Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

microRNAs （miRNAs） have played a key role in human tumorigenesis, tumor progression, and metastasis. On the one hand, miRNAs are aberrantly expressed in many types of human cancer; on the other hand, miRNAs can function as tumor suppressors or oncogenes that target many cancer-related genes. This study aimed to investigate the effects of miRNA-200c （miR-200c） on the biological behavior and mechanism of proliferation, migration, and invasion in the prostate cancer cell line Du145. In this study, Du145 cells were transfected with miR-200c mimics or negative control miR-NC by using an X-tremeGENE siRNA transfection reagent. The relative expression of miR-200c was measured by RT-PCR. The proliferation, migration, and invasion abilities of Du145 cells were detected by CCK8 assays, migration assays and invasion assays, respectively. The expressions of ZEB1, E-cadherin, and vimentin were observed by western blot. Results showed that DU145 cells exhibited a high expression of miR-200e compared with immortalized normal prostate epithelial cell RWPE-1. Du145 cells were then transfected with miR-200c mimics and displayed lower abilities of proliferation, migration, and invasion than those transfected with the negative control. The protein levels of ZEB1 and vimentin were expressed at a low extent in Du145 cells, which were transfected with miR-200c mimics; by contrast, E-cadherin was highly expressed. Hence, miR-200c could significantly inhibit the proliferation of the prostate cancer cell line Du145; likewise, miR- 200c could inhibit migration and invasion by epithelial-mesenchymal transition.

关键词： miR-200c proliferation migration invasion prostate cancer Du145 cell ZEB1

Distribution of Different Biogeographical Tintinnids in Yellow Sea and Bohai Sea

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Journal of Ocean University of China 2018年第2期17卷 371-384页

作者： CHEN Xue LI Haibo ZHAO Yuan ZHAO Li DONG Yi ZHANG Wuchang xiao tian CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of Oceanology Chinese Academy of Sciences Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology College of Earth Sciences University of Chinese Academy of Sciences

There were different biogeographical tintinnids in the oceans. Knowledge of their distribution pattern and mixing was important to the understanding of ecosystem functions. Yellow Sea(YS) and Bohai Sea(BS) were semi-enclosed seas influenced by warm water intrusion and YS cold bottom water. The occurrence of tintinnids in YS and BS during two cruises(summer and winter) were investigated to find out: i) whether warm-water tintinnids appeared in YS and BS; ii) whether boreal tintinnids appeared in high summer; iii) the core area of neritic tintinnids and iv) how these different biogeographical tintinnids mixed. Our results showed that tintinnid community was dominated by neritic tintinnid. We confirmed the occurrence of warm-water tintinnids in summer and winter. In summer, they intruded into BS and mainly distributed in the upper 20 m where Yellow Sea Surface Warm Water(YSSWW) developed. In winter, they were limited in the surface water of central deep region(bottom depth >50 m) of YS where were affected by Yellow Sea Warm Water(YSWW). Boreal tintinnids occurred in YS in high summer(August) and in winter, while they were not observed in BS. In summer, the highest abundance of boreal tintinnids occurred in Yellow Sea Bottom Cold Water, indicating the presence of an oversummering stock. In winter, they were concentrated in the north of YSWW. Vertically, neritic tintinnids abundance was high in the bottom layers. Horizontally, high neritic tintinnids abundance in bottom layers occurred along the 50 m isobath coinciding with the position of front systems. Front systems were the core distribution area of neritic tintinnids. High abundance areas of warm-water and boreal tintinnids were clearly separated vertically in summer, and horizontally in winter. High abundance of neritic tintinnids rarely overlapped with that of warm-water or boreal tintinnids.

关键词： tintinnids biogeography warm-water boreal neritic Yellow Sea Bohai Sea

Functional Characterization of a NEM1-Like Gene in Magnaporthe oryzae

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Agricultural Sciences in China 2011年第9期10卷 1385-1390页

作者： WANG Ying JIAO tian-lei LIU xiao-hong LIN Fu-cheng WU Wei-ren Department of Agronomy College of Agriculture ＆ Biotechnology Zhejiang University Hangzhou 310029 P.R.China Institute of Biotechnology College of Agrieulture ＆ Biotechnology Zhejiang University Hangzhou 310029 PR. China School of Life Sciences Fujian Agriculture ＆ Forestry University Fuzhou 350002 P.R. China

Magnaporthe oryzae, a filamentous ascomycete fungus, is well known as the causal agent of rice blast. With the technology of suppression subtractive hybridization （SSH）, it was previously found that MGG_06001 （or named MoNEM1）, a gene of M. oryzae homologous to the NEM1 （nuclear envelope morphology protein 1） gene of baker＇s yeast （Saccharomyces cerevisiae）, is differentially expressed between the mature appressium and the conidium and mycelium. This study aimed to characterize the function of MoNEM1 gene by knocking it out using the method of target gene replacement. The AMoneml mutants exhibited reduced mycelial growth and conidiation. However, disruption of MoNEM1 gene does not affect the pathogenicity of M. oryzae on barley and rice.

关键词： Magnaporthe oryzae MoNEM1 conidiation growth pathogenicity

食管癌内镜黏膜下剥离术后发生食管狭窄风险列线图构建与验证

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海南医学 2024年第8期35卷 1185-1189页

作者：张冰白璟窦晓雅苏鹏飞南阳市中心医院胸外科食管病区河南南阳473000 南阳市中心医院泌尿外科河南南阳473000 南阳市中心医院感染科河南南阳473000

目的探讨影响食管癌内镜黏膜下剥离术(ESD)术后发生食管狭窄的危险因素,并建立其风险列线图预警模型。方法回顾性分析2022年1月至2023年5月于南阳市中心医院行ESD治疗的94例食管癌患者的临床资料,依据患者术后是否发生食管狭窄残余将其... 详细信息

目的探讨影响食管癌内镜黏膜下剥离术(ESD)术后发生食管狭窄的危险因素,并建立其风险列线图预警模型。方法回顾性分析2022年1月至2023年5月于南阳市中心医院行ESD治疗的94例食管癌患者的临床资料,依据患者术后是否发生食管狭窄残余将其分为狭窄组(n=20)和非狭窄组(n=74),比较两组患者的临床资料,采用二元Logistic回归分析法分析食管癌患者ESD术后食管狭窄的影响因素,构建列线图风险模型并进行验证。结果经单因素分析结果显示,年龄、性别、手术时间、食管癌家族史、病变部位、病理类别与食管癌患者ESD术后食管狭窄无关(P>0.05),而ESD手术史、病变环周范围、浸润深度、术后体位干预、创面材料覆盖、SEMS置入、术后抗炎药物使用、术后抗纤维化使用、病变纵径与食管癌患者ESD术后食管狭窄有关(P<0.05);经二元Logistic回归分析显示,病变环周≥3/4周、浸润深度m3~sm1、术后平卧体位、病变纵径≥50mm是食管癌患者ESD术后食管狭窄的独立危险因素(P<0.05),而SEMS置入、术后使用抗炎药物、术后使用抗纤维化药物是独立保护因素(P<0.05);Hosmer-Leme-show拟合优度检验结果显示,χ^(2)=2.263,P=0.972,受试者工作特征曲线(ROC)的曲线下面积为0.971(P<0.001,95%CI:0.940~1.000),灵敏度为95.00%,特异性为91.90%,最大约登指数为0.869,模型预测食管癌患者ESD术后食管狭窄发生的校准曲线总体与理想曲线趋势相同;模型回归方程Logit(P)=6.666×病变环周范围+4.399×浸润深度+3.938×术后体位干预-3.534×SEMS置入-3.891×术后抗炎药物使用-4.871×术后抗纤维化药物使用+3.513×病变纵径-6.577。结论食管癌患者ESD术后食管狭窄的发生受疾病和多种术后护理因素的影响,根据其危险因素建立的风险列线图预警模型具有较好的区分和预测能力,可为食管狭窄患者行ESD术后预防食管狭窄发生提供参考方案。

关键词：食管癌内镜黏膜下剥离术食管狭窄危险因素风险列线图模型

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