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Molecular Design and Immunogenicity of a Multiple-epitope FMDV Antigen and DNA Vaccination

Chemical Research in Chinese Universities 2008年第1期24卷 69-74页

作者： MA ming-xiao JIN Ning-yi LIU Hui-juan ZHENG Mini YIN Ge-fen tian ming-yao JIN ming-lan LU Hui-jun SHEN Guo-shun ZHU Guang-ze JIN Hong-tao JIN Kuo-shi ZHANG Ying-jiu Genetic Engineering Laboratory Academy of Military Medical Seienees Changehun 130062 P. R. China College of Animal Seienee and Veterinary Medieine Liaoning Medieal University Jinzhou 121001 P. R. China College of Animal Seienee and Veterinary Medieine Yunnan Agrieultural University Kunming 650201 P R. China Key Laboratory for Moleeular Enzymology and Engineering of Ministry of Edueation Jilin University Changehun 130021 P. R. China

This article reports the design and construction of a multiple-epitope foot and mouth disease virus （FMDV） antigen, designated as OAAT. This recombinant antigen consists of the structural protein VP1 genes from serotypes A and O FMDV, five major VP1 immunodominant epitopes from two genotypes of Asial serotype, and three Th2 epitopes originating from the nonstructural protein, three ABC gene and structural protein VP4 gene. Expressions of target gene from these plasmids in HeLa cells were verified by Western-blot. BALB/c mice were immunized intramuscularly with the DNA vaccines thrice every two weeks. We found that pA could induce simultaneously specific antibodies against serotypes A, Asial, and O FMDV. Compared to those of the controls, the spots of FMDV-specific IFN-7 and cytotoxic activity from mice immunized with pA were significantly increased, pA provided full protection in 2/4 guinea pigs from challenge with FMDV O/NY00 and Asial/YNBS/58, respectively. The results show that although pA did not give full protection in 100% immunized guinea pigs from challenge with type O and Asial FMDV, respectively, OAAT may be potential immunogen against FMDV and pA may be potential DNA vaccines against FMDV.

关键词： Multiple-epitope Foot and mouth disease virus DNA vaccine

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MAGNETIC　INVESTIGATION　OF　THE　NICKEL　COMPLEXES　OF　MULTI－SULFUR　1．2－DITHIOLATES

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Chinese Chemical Letters 1996年第7期7卷 685-686页

作者： tian ming yao Xian Fa SHI Xiao Zeng YOU(Department of Chemistry,Tongji University, Shanghai 200092)(State Key Laboratory of Coordination Chemistry,Nanjing University,Nanjing 210093) TONGJI UNIV DEPT CHEMSHANGHAI 200092PEOPLES R CHINA NANJING UNIV STATE KEY LAB COORDINAT CHEMNANJING 210093PEOPLES R CHINA

The magnetic susceptibility of (Bu4N)(NI(MEDT)2) and (Bu4N)(Ni(PHDT)2)(MEDT:5-methyl-6-hydro-1, 4-dithiin-2, 3-dithiolate, PHDT: 5-phenyl-6-hydro-1.4-dithiin- 2,3-dithiolate) was measured in the temperature range of 75-300K. The magnitudeof magnctic moments is consistent,with a doublet state arising from Ni(Ⅲ).Antiferromagnetic properties were discussed in consideration of molecularstructure.

关键词： MULTI DITHIOLATES INVESTIGATION MAGNETIC COMPLEXES NICKEL OF SULFUR THE

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Cardioprotective effects of Guanxinshutong （GXST） against myocardial ischemia/ reperfusion injury in rats

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Journal of Geriatric Cardiology 2012年第2期9卷 130-136页

作者： Zhuo Llang Li-Feng Llu tian-ming yao Yu Huo Ya-Ling Han Department of Cardiology Shenyang Northern Hospital Shenyang 110016Liaoning ProvinceChina Department of Cardiology Xijing HospitalThe Fourth Military Medical UniversityXi'an 710032Shaanxi ProvinceChina Department of lnstitute of Geriatric Cardiology Chinese PLA General Hospital 28 Fuxing Road100853 BeijingChina

Background The protective effects against reperfusion injury of cardioprotective drugs have recently been evaluated and found to be inadequate. Guanxinshutong （GXST）, a combination of the traditional herb and Mongolian medicine, is effective and safe in treating angina pectoris in clinical trials. We assess the cardioprotective effects of GXST against myocardial ischemia and reperfusion （MI/R） injury in rats and explore its possible mechanism. Methods Forty-five male Sprague Dawley rats were randomized into three groups： non-MlfR group （Sham, n = 15）, MUR group treated with vehicle （Control, n = 15） and MI/R group treated with GXST （Drug, n = 15）. MI/R was induced by ligation of the left anterior descending coronary artery （LAD） for 30 minutes, followed by 2/24 hour reperfusion in the Control and Drug groups. In the Sham group, the LAD was exposed without occlusion. GXST powder （in the Drug group） or saline （in the Control and Sham groups） were administered via direct gastric gavage from 7 day prior to surgery. Blood samples were collected from the carotid artery （10 rats each group） after 2 hours of reperfusion, to determine the levels of tumor necrosis factor-or （TNF-ct）, interleukin-1 ~ （IL-113）, interleukin-6 （IL-6） and intercellular adhesion molecule-1 （ICAM-1） using enzyme-linked immunosorbent assays. The animals were then sacrificed and the hearts were harvested for histopathology and western blot analysis. Infarct size was measured in the remaining five rats in each group after 24 hours reperfusion. Results GXST significantly decreased levels of TNF-ct, IL-1β, IL-6, ICAM-1, apoptosis index （AI） and infarct size. GXST also obviously inhibited nuclear factor kappa B （NF.r,B） activity when compared with the Control group （all P 〈 0.05）. Conclusions GXST is effective in protecting the myocardium against MI/R injury in rats. Its possible cardioprotective mechanism involves inhibition of the inflammatory response and apoptosis following MI/R injury.

关键词： I/R injury NF-r.B Inflammation Apoptosis Chinese herbal Guanxinshutong

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Discussion of the feasibility about the damage control surgery applied in the comprehensive surgical treatment shelter

Discussion of the feasibility about the damage control surge...

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中华医学会第十五次全国心血管病学大会

作者： Meiling Ge ming Liang tian-ming yao Jing-Yang Sun Ya-Ling Han Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital Department of Cardiology The Second Affiliated Hospital of Dalian Medical University

Background The damage control surgery was gradually paid more attention and advocated in the field of treatment of trauma in resent years. Owning to the limitations of the medical conditions in our army field , the su...

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Protection Evaluation of X-ray Radiation in the Comprehensive Surgical Treatment Shelter

Protection Evaluation of X-ray Radiation in the Comprehensiv...

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中华医学会第十五次全国心血管病学大会

作者： Meng Wang ming Liang tian-ming yao Jing-yang Sun Huiyu Shao Ya-ling Han Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital Department of Cardiology The Second Affiliated Hospital of Dalian Medical University

Background Natural disasters have been frequent in recent years Effective treatment of patients with cardiovascular disease following natural disasters is an unsolved problem We aimed to develop a comprehensive surgic...

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Safety Evaluation of X-ray Radiation in the Comprehensive Surgical Treatment Shelter

Safety Evaluation of X-ray Radiation in the Comprehensive Su...

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中华医学会第十五次全国心血管病学大会

作者： Meng Wang ming Liang tian-ming yao Jing-yang Sun Huiyu Shao Ya-ling Han Liberation Army Shenyang Northern Hospital Department of Cardiology The Second Affiliated Hospital of Dalian Medical University

Background Natural disasters have been frequent in recent years. Effective treatment of patients with cardiovascular disease following natural disasters is an unsolved problem. We aimed to develop a comprehensive surg...

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Development and application of vehicle-mounted medium C shaped brachial angiography machine system

Development and application of vehicle-mounted medium C shap...

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中华医学会第十五次全国心血管病学大会

作者： Jingyang Sun ming Lian tian-ming yao Jian-Hao Yu Dong-Hui Wang Ya-Ling Han Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital Beijing Smart Image Technology Company Limited

Background There is no report about the vehicle-mounted medium C shaped brachial angiography machine system. This styday adapt to the field of minimally invasive operation module to achieve its interventional operatio...

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Investigation of X-radiation dose to subjects in the comprehensive surgical treatment field shelter during interventional therapy

Investigation of X-radiation dose to subjects in the compreh...

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中华医学会第十五次全国心血管病学大会

作者： Huiyu Shao ming Liang Xiao-Zeng Wang tian-ming yao Jing-Yang Sun Geng Wang Meng Wang Ya-Ling Han Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital

Background Comprehensive surgical treatment field shelter are developed by our center used in field support and disaster emergency rescue. Intervention, minimally invasive and conventional surgery as the main means of this system, to belly department, limbs and skull important viscera urgent heavy damage and disease can be effectively damage control treatment. But in the shelter within the intervention in the process

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Nanocarrier-mediated siRNA delivery:a new approach for the treatment of traumatic brain injury-related Alzheimer's disease

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Neural Regeneration Research 2025年第9期20卷 2538-2555页

作者： Jie Jin Huajing Zhang Qianying Lu Linqiang tian Sanqiao yao Feng Lai Yangfan Liang Chuanchuan Liu Yujia Lu Sijia tian Yanmei Zhao Wenjie Ren Institute of Disaster and Emergency Medicine Tianjin UniversityTianjinChina Key Laboratory for Disaster Medicine Technology TianjinChina Henan Medical Key Laboratory for Research of Trauma and Orthopedics The Third Affiliated Hospital of Xinxiang Medical UniversityXinxiangHenan ProvinceChina Clinical Medical Center of Tissue Engineering and Regeneration Xinxiang Medical UniversityXinxiangHenan ProvinceChina Institutes of Health Central Plain Xinxiang Medical UniversityXinxiangHenan ProvinceChina School of Public Health Xinxiang Medical UniversityXinxiangHenan ProvinceChina

Traumatic brain injury and Alzheimer's disease share pathological similarities,including neuronal loss,amyloid-βdeposition,tau hyperphosphorylation,blood-brain barrier dysfunction,neuroinflammation,and cognitive ***,traumatic brain injury can exacerbate Alzheimer's disease-like pathologies,potentially leading to the development of Alzheimer's *** offer a potential solution by facilitating the delive ry of small interfering RNAs across the blood-brain barrier for the targeted silencing of key pathological genes implicated in traumatic brain injury and Alzheimer's disease.U nlike traditional approaches to neuro regeneration,this is a molecula r-targeted strategy,thus avoiding non-specific drug *** review focuses on the use of nanocarrier systems for the efficient and precise delive ry of siRNAs,discussing the advantages,challenges,and future *** principle,siRNAs have the potential to target all genes and non-targetable protein s,holding significant promise for treating various *** the various therapeutic approaches currently available for neurological diseases,siRNA gene silencing can precisely"turn off"the expression of any gene at the genetic level,thus radically inhibiting disease progression;however,a significant challenge lies in delivering siRNAs across the blood-brain *** have received increasing attention as an innovative drug delive ry tool fo r the treatment of brain *** are considered a potential therapeutic strategy with the advantages of being able to cross the blood-brain barrier,targeted drug delivery,enhanced drug stability,and multifunctional *** use of nanoparticles to deliver specific modified siRNAs to the injured brain is gradually being recognized as a feasible and effective *** this strategy is still in the preclinical exploration stage,it is expected to achieve clinical translation in the future,creating a new field of molecular tar

关键词： blood-brain barrier cognitive dysfunction exosomes gene silencing immunotherapy inorganic nanomaterials liposome nanoparticles neuroinflammation polymer nanoparticles stem cells

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Animal study of application of kelp micro gelation embolization for hepatic artery rupture

Animal study of application of kelp micro gelation embolizat...

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中华医学会第十五次全国心血管病学大会

作者： Yabin Liu ming Liang Guo-jun Lu tian-ming yao Jing-yang Sun Wei Wang Xiao-jun Ma Ya-ling Han Dalian Institute of Chemical Physics Chinese Academy of Sciences Department of Cardiology Institute of Cardiovascular Research of People’s Liberation Army Shenyang Northern Hospital

Background Effective treatment of patients with vascular disease during war or natural disasters is a worldwide problem. We aimed to develop a novel field injuries surgical treatment cabin to provide emergency interventional services for patients with critical cardiovascular disease following war or natural disasters.

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