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A Glycoside Hydrolase 12 Protein from Cytospora chrysosperma Triggers Plant Immunity but is not Essential to Virulence

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A Glycoside Hydrolase 12 Protein from Cytospora chrysosperma...

中国植物病理学会2024年学术年会

作者： XU Zhiye LUO Zheng XIONG Dianguang GAO Meng tian chengming Beijing Key Laboratory for Forest Pest Control Beijing Forestry University The Key Laboratory for Silviculture and Conservation of Ministry of Education College of ForestryBeijing Forestry University

Phytopathogens secrete numerous effectors that facilitate their infection and colonization ***,the pathogenicmechanism of effectors in ***,the causal agent of canker disease in many woody plants,remains poorly *** this study,we identified five glycoside hydrolase family 12(GH12) effectors in *** genome,all of which were significantly upregulated during the infection *** them,CcEGl,which contains an additional carbohydrate-binding module family 1 domain(CBM1) at the C. terminal,was selected for further *** expression showed that CcEGl was localized to the apoplasticregion of Nicotiana benthamiana and acted as an elicitor to induce cell death,and activate the expression of genes involved in salicylicacid,jasmonic acid,and ethylene signaling ***,the GH12 domain was sufficient for cell death-inducing activity,rather than the CBM1 ***,the leucine-rich repeat(LRR) receptor-like kinases NbBAKl and NbSOBIRl were required for defense responses triggered by ***,deletion of CcEGl did not affect fungal pathogenicity,growth,response to hydrogen peroxide and cell wall integrity agents,but affected cellulase *** conclusion,our results suggest that CcEGl induces coreceptors NbBAK1-and NbSOBIR1-dependent plant immunity,increasing our understanding about fungal pathogenesis during the interaction between *** and its host.

关键词： A Glycoside Hydrolase 12 Protein from Cytospora chrysosperma Triggers Plant Immunity but is not Essential to Virulence

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生于华中五味子上的5种拟盘多毛孢属真菌

菌物学报 2024年第5期43卷 24-40页

作者：袁榕彭骋李曙吉田呈明北京林业大学林学院省部共建森林培育与保护教育部重点实验室北京100083

华中五味子Schisandraphenanthera是一种重要的药用植物资源。拟盘多毛孢属Pestalotiopsis真菌种类丰富,分布广泛,是重要的病原菌、内生真菌及腐生菌。本研究采用组织分离法从采集自陕西省安康市宁陕县的华中五味子上分离获得10株拟盘... 详细信息

华中五味子Schisandraphenanthera是一种重要的药用植物资源。拟盘多毛孢属Pestalotiopsis真菌种类丰富,分布广泛,是重要的病原菌、内生真菌及腐生菌。本研究采用组织分离法从采集自陕西省安康市宁陕县的华中五味子上分离获得10株拟盘多毛孢属真菌,结合形态学观察与基于ITS、tef1及tub23个位点的多基因序列的分子系统学分析,将这些菌株鉴定为冷杉拟盘多毛孢***、江西拟盘多毛孢***、庐山拟盘多毛孢***、稻拟盘多毛孢***以及新种五味子拟盘多毛孢***,上述拟盘多毛孢属真菌为华中五味子上首次报道。

关键词：形态学分子系统学新种分类

Modeling the dynamics of a spruce forest and dwarf mistletoe population:a coupled system

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Journal of Forestry Research 2021年第4期32卷 1579-1585页

作者： Dianguang Xiong Zhouyuan Li Yingmei Liang chengming tian College of Forestry Beijing Forestry UniversityBeijing 100083People’s Republic of China Institute of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijing 100871People’s Republic of China Museum of Beijing Forestry University Beijing Forestry UniversityBeijing 100083People’s Republic of China

The parasitic plant dwarf mistletoe(Arceuthobium) is currently one of the most threatening infestations of coniferous forests worldwide,especially in Eurasia and North America,but its population dynamics in relation to one of its hosts(spruce) remain ***,toward understanding the population dynamics,differential equations were used to construct a life history model for the two populations,and two relatively independent subsystems,host and parasite,were generated from their symbiotic relationships.A suspected-infection model was used to couple *** resulting models were used to analyze structural changes in the *** each infected spruce was assumed to support 1000 parasite shoots,the spruce population first increased rapidly,then *** 2000 parasite shoots were assumed,the forest declined dramatically,slipping to zero in the 10 th year,and the spruce seedlings were unable to *** shoot population curves transformed from exponential J-shapes to logistic S-shapes,reaching population limitations as germination rates *** results provide important clues to understanding developmental trends of the present parasite population and will assist in reconstructing invasion histories.

关键词： Dwarf mistletoe Spruce Population dynamic model

杨树腐烂病菌SNARE蛋白CcNyv1的功能

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林业科学 2024年第9期60卷 90-98页

作者：邱啸林王姝敏余璐杨宇辰熊典广田呈明北京林业大学林学院北京100083

【目的】解析SNARE蛋白Nyv1在杨树腐烂病菌中调控囊泡运输相关过程的功能,探究该病菌在生长发育和致病过程中的作用机制,为阐明SNARE蛋白在杨树腐烂病菌致病过程中的调控机制提供理论依据,并为该病害防控提供参考。【方法】1)利用酿酒酵... 详细信息

【目的】解析SNARE蛋白Nyv1在杨树腐烂病菌中调控囊泡运输相关过程的功能,探究该病菌在生长发育和致病过程中的作用机制,为阐明SNARE蛋白在杨树腐烂病菌致病过程中的调控机制提供理论依据,并为该病害防控提供参考。【方法】1)利用酿酒酵母Nyv1的氨基酸序列进行Blastp分析,获得其同源的CcNyv1基因;2)利用生物信息学软件对CcNyv1编码的氨基酸序列和其他真菌中的Nyv1同源序列进行多序列比对,并构建系统发育树;3)采用Split-marker方法和PEG介导的遗传转化体系,构建CcNyv1基因缺失突变体及其回补菌株;4)通过PDA平板生长试验观察菌丝生长速率,分析CcNyv1基因及其Longin、SNARE结构域对菌丝的营养生长和盐胁迫响应的影响;5)通过对1年生杨树枝条烫伤接种观察病斑扩展面积,评价CcNyv1基因及其Longin、SNARE结构域对杨树腐烂病菌致病力的影响;6)利用FM4-64染剂染色,探究CcNyv1基因和Longin、SNARE结构域对该病菌的内吞作用产生的影响。【结果】1)通过酿酒酵母Nyv1的氨基酸序列blast比对,在杨树腐烂病菌中获得其同源基因CcNyv1,该基因全长780 bp,包含1个内含子,编码1个233 aa的囊泡运输相关R-SNARE蛋白,该蛋白含有1个N端的Longin结构域和1个C端的SNARE结构域;2)多序列对比和系统发育分析表明,CcNyv1和其他子囊菌中的Nyv1同源基因具有较高的序列相似度,且在进化上较为保守,与寄生隐丛赤壳中的同源基因的亲缘关系最为接近;3)成功构建CcNyv1基因缺失突变体、Longin结构域缺失突变体、SNARE结构域缺失突变体和CcNyv1基因回补菌株;4)菌丝生长表型和侵染杨树枝条致病表型分析表明,CcNyv1基因缺失突变体和Longin、SNARE结构域缺失突变体在菌丝营养生长、盐胁迫响应以及致病力等方面存在明显缺陷;5)比较FM4-64染色后野生型和缺失突变体菌株的囊泡分布情况,可见CcNyv1基因和Longin、SNARE结构域影响杨树腐烂病菌的内吞作用。【结论】杨树腐烂病菌SNARE蛋白CcNyv1在营养生长、胁迫应答、致病力及内吞作用等方面发挥重要调控作用。

关键词：杨树腐烂病金黄壳囊孢囊泡运输 SNARE CcNyv1 致病性

基于系统动力学的松材线虫病防治效果模型构建

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西北林学院学报 2024年第5期39卷 14-21,67页

作者：王炳鑫肖杰文邓子鑫熊典广周长富田呈明北京林业大学林学院北京100083 浏阳市林业局湖南浏阳410300

利用系统动力学方法,通过对松材线虫病不同防治措施的防治效果进行调查,建立松材线虫病防治效果预测动力学模型,对不同防治措施下病害未来发生情况和改变政策方案后的防治效果进行模拟研究,结果表明,通过模型仿真能够预测不同防治方法... 详细信息

利用系统动力学方法,通过对松材线虫病不同防治措施的防治效果进行调查,建立松材线虫病防治效果预测动力学模型,对不同防治措施下病害未来发生情况和改变政策方案后的防治效果进行模拟研究,结果表明,通过模型仿真能够预测不同防治方法下未来几年的防治效果,通过仿真测定显示采取增加飞防覆盖率、增加经费投入、提供飞防和砍伐补贴等政策,可以实现有效治理松材线虫疫情的目的。综合来看,运用系统动力学方法进行不同防治模式的评价和优化选择,能够为松材线虫疫情防治方案的制定提供一定参考。

关键词：松材线虫病模型治理政策仿真

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Phylogeny and taxonomy of the genus Elsino?（Myriangiales, Dothideomycetes）

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Phylogeny and taxonomy of the genus Elsino?（Myriangiales, D...

中国菌物学会第七届全国会员代表大会暨2017年学术年会

作者： Xinlei Fan chengming tian the Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University

The genus Elsino? includes phytopathogens causing scab and anthracnose disease on many economically important plants, such as citrus, grapevines, field crops and woody hosts. Current criteria for species recognition and host specificity in Elsino? are unclear due to overlapping morphological characteristics, and the lack of molecular and pathogenicity data. In the present study we revised the taxonomy of Elsino? based on DNA sequence and morphological data derived from 119 isolates,representing 67 host genera from 17 countries, including 64 ex-type cultures. Combined analyses of ITS, LSU, rpb2 and TEF1-α DNA sequence data were used to reconstruct the backbone phylogeny of the genus Elsino?. Based on the single nomenclature for fungi, 26 new combinations are proposed in Elsino? for species that were originally described in Sphaceloma. A total of 13 species are epitypified with notes on their taxonomy and phylogeny. A further eight new species are introduced, leading to a total of 75 Elsino? species supported by molecular data in the present study. For the most part species of Elsino? appear to be host specific, although the majority of the species treated are known only from a few isolates, and further collections and pathogenicity studies will be required to reconfirm this conclusion.

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Influencing Factors and Process on in SituDegradation of Poly(Butylene Succinate) Film by Strain Bionectria ochroleucaBFM-X1 in Soil

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Journal of Environmental Protection 2012年第6期3卷 523-532页

作者： Xueli Mei chengming tian Qian Dong Yingmei Liang College of Environmental Science & Engineering Beijing Forestry University Beijing China Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University Beijing China Museum of Beijing Forestry University Beijing Forestry University Beijing China

This is the first report on the PBS film degraded by any Bionectria ochroleuca fungal strain. The fungal strain BFM-X1 was isolated from an air environment on a vegetable field and was capable of degrading poly(butylene succinate) (PBS). The taxonomic identity of the strain BFM-X1 was confirmed to be Bionectria ochroleuca (showing a 99% similarity to B. ochroleuca in a BLAST search) through an ITS rRNA analysis. The bio-degradation of the PBS film by strain BFM-X1 was studied. Approximately 97.9% of the PBS film was degraded after strain BFM-X1 was inoculated at 28?C for 14 days. The degradation efficiency of BFM-X1 against PBS film under different soil environmental conditions was characterized. The results indicated that 62.78% of the PBS film loss was recorded in a 30-d experimental run in a sterile soil environment indoors. On adding strain BFM-X1 to a soil sample, the PBS degradation rate accelerated approximately fivefold. Furthermore, both temperature and humidity influenced the in situ degradation of the PBS by strain BFM-X1, and temperature may be the major regulating factor. The degradation was particularly effective in the warm season, with 90% of weight loss occurring in July and August. Scanning electron microscope observations showed surface changes to the film during the degradation process, which suggested that strain BFM-X1preferentially degraded an amorphous part of the film from the surface. These results suggested that the strain B. ochroleuca BFM-X1 was a new resource for degrading PBS film and has high potential in the bioremediation of PBS-plastic-contaminated soil

关键词： Poly(Butylene Succinate) (PBS) In Situ Biodegradation Strain B. ochroleuca BFM-X1 Temperature Humidity

Families and genera of diaporthalean fungi associated with canker and dieback of tree hosts in Dongling Mountain of China

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Families and genera of diaporthalean fungi associated with c...

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Haiyan Zhu chengming tian Xinlei Fan The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University

Diaporthales is an order comprising of important plant pathogens, saprobes and endophytes on a wide range of woody hosts. Members of this order cause various serious diseases, e.g., chestnut blight disease caused by Cryphonectria parasitica;stem-end rot of citrus fruits infected by Diaporthe citri;poplar and willow canker disease caused by Cytospora chrysosperma;birch canker and dieback caused by Melanconis stilbostoma;leading to severe ecological and economic losses worldwide. Identification of these pathogens is difficult due to their similar disease symptoms and various morphological characters. In the current study we obtained 35 representative isolates from 12 tree hosts of Betulaceae, Cupressaceae, Fagaceae, Juglandaceae, Rosaceae,Tiliaceae and Ulmaceae in Dongling Mountain of China. A total of 16 species residing in five families of Diaporthales(Cytosporaceae, Diaporthaceae, Erythrogloelaceae, Melanconidaceae and Juglanconidaceae) were identified. These include eight known species(Cytospora leucostoma, C. pruinopsis, C. vinacea, Diaporthe betulina, D. eres, D. rostrata, Juglamconis oblonga and Melanconis stilbostoma) and eight novel species(Cytospora albodisca, C. coryleae, C. discostoma, C. donglinensis, C. querceae, C. spiraeicola, C. verrucosa and Dendrostoma donglinensis) based on morphological descriptions and phylogenetic analyses using partial ITS,LSU, act1, cal, his, rpb2, tef1-α and tub2 gene sequences. The majority of the species investigated here appear to be host-specific, although further collections and pathogenicity studies will be required to confirm this *** results represent the first attempts to study diaporthalean fungi associated with canker and dieback of tree hosts in Dongling Mountain of China.

关键词： Ascomycota Diaporthales Phylogeny Taxonomy

Cytospora species associated with canker and dieback disease of Rosaceae in China

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Cytospora species associated with canker and dieback disease...

多彩菌物美丽中国——中国菌物学会2019年学术年会

作者： Meng Pan chengming tian Xinlei Fan The Key Laboratory for Silviculture and Conservation of Ministry of Education Beijing Forestry University

Cytospora canker is a destructive disease of numerous hosts and causes serious economic losses with a worldwide distribution. Identification of Cytospora species is difficult due to insufficient phylogenetic understanding and overlapped morphological characteristics. In this study, 48 fungal strains which covered nine genera of Rosaceae were isolated from branches or twigs with symptomatic canker and dieback disease in *** morphological observation and multilocus phylogeny of ITS, LSU, act, rpb2, tef1-α and tub2 gene regions, indicating 21 distinct lineages with high branch support. These include ten Cytospora species were new,i.e., C. cinnamoneus, C. cotoneastricola, C. ochreae, C. olivaceae, C. pruni-mume, C. roseae, C. rosicola, ***, C. sorbina, and C. tibetensis, and 11 known taxa include Cytospora erumpens, C. japonica, ***, C. leucostoma, C. mali, C. parasitica, C. populinopsis, C. schulzeri, C. sibiraeae, C. spiraeae and ***. This study provides an initial understanding of the taxonomy of Cytospora associated with canker and dieback disease of Rosaceae in China. The plants of Rosaceae are important ecological and economic tree species in China. The current study indicates that the incidence of Cytospora species is serious and have different symptoms in various hosts, including 20 host species of 9 genera in Rosaceae, i.e. Cotoneaster, Crataegus, Malus,Prunus, Pyrus, Rosa, Sibiraea, Sorbus and Spiraea. The result coincides with previous reports that widely extended Cytospora species have been identified to occur in many host species in the current study. Furthermore,Cytospora cotoneastricola, C. ochreae, C. tibetensis were all collected from Cotoneaster sp., which indicates that the same host could be infected by more than one species. At present, China is a hot place to study these taxa as many species of Cytospora are isolated from important hosts such as Rosaceae. Thus the further studies are required to discover the spe

关键词： Cytosporaceae Rosaceae taxonomy