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Comparative evaluation of microscopy,OptiMAL® and 18S rRNA gene based multiplex PCR for detection of Plasmodium falciparum & Plasmodium vivax from field isolates of Bikaner,India

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Asian Pacific Journal of Tropical Medicine 2013年第5期6卷 346-351页

作者： Deepak Pakalapati Shilpi garg Sheetal Middha Abhishek Kochar Amit kumar Subudhi Boopathi Pon Arunachalam Sanjay kumar Kochar Vishal Saxena Pareek RP Jyoti Acharya Dhanpat kumar Kochar Ashis Das Center for Biotechnology Dept of Biological SciencesBirla Institute of Technology and SciencePilani-333031RajasthanIndia Department of Medicine Sardar Patel Medical CollegeBikaner-334003RajasthanIndia Medical Centre Birla Institute of Technology and SciencePilani-333031RajasthanIndia Kothari Medical and Research Institute BikanerRajasthan-334003India

Objective:To evaluate microscopy,OptiMAL? and multiplex PCR for the identification of Plasmodium falciparum（***） and Plasmodium vivax（***） from the field isolates of Bikaner,Rajasthan（Northwest India）.Methods:In this study,a multiplex PCR（*** and ***） was further developed with the incorporation of Plasmodium malariae（***） specific primer and also a positive *** performance of microscopy,plasmodium lactate dehydrogenase（pLDH） based malaria rapid diagnostic test OptiMAL? and 18S rRNA gene based multiplex PCR for the diagnosis of *** and *** was ***:The three species multiplex PCR ***,*** and ***) with an inbuilt positive control was developed and *** comparison with multiplex PCR,which showed the sensitivity and specificity of 99.36%（95%CI,98.11%-100.00%） and 100.00%（95%CI,100.00%-100.00%）,the sensitivity and specificity of microscopy was 90.44%（95%CI,88.849-95.04%） and 99.22%（95% CI,97.71%-100.00%）,and OptiMAL? was 93.58%（95%CI,89.75%-97.42%） and 97.69%（95%CI, 95.10%-100.00%）.The efficiencies were 99.65%,95.10%and 95.45%for multiplex *** and OptiMAL?.***:Our results raise concerns over the overall sensitivities of microscopy and OptiMAL?,when compared to the multiplex PCR and thus stress the need for new molecular interventions in the accurate detection of the malarial *** further highlights the fact that further developments are needed to improve the performance of rapid diagnostic tests at field level.

关键词： Malaria diagnosis Microscopy OptiMAL® Multiplex PCR 18S rRNA gene Plasmodium

Status epilepticus and acute promyelocytic leukemia

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Journal of Acute Disease 2017年第5期6卷 235-236页

作者： Sunil kumar garg Pragya garg Dubai NMC Hospital Dubai

Neurological manifestations are rare as initial presentation in acute promyelocytic leukemia (APL). This case illustrates status epilepticus as initial presentation in this disease. Atypical cells on peripheral smear ... 详细信息

关键词： Acute promyelocytic leukemia Acute stroke Status epilepticus Atypical cells

Accuracy of preoperative CT liver volumetry in living donor hepatectomy and its clinical implications

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Hepatobiliary Surgery and Nutrition 2018年第3期7卷 167-174页

作者： Sanjay Goja Sanjay kumar Yadav Amardeep Yadav tarun Piplani Amit Rastogi Prashant Bhangui Sanjiv Saigal Arvinder Singh Soin Institute of Liver Transplant and Regenerative Medicine MedantaThe MedicityGurgaonDelhi(NCR)India Department of Radiology and Nuclear Medicine MedantaThe MedicityGurgaonDelhi(NCR)India

Background: An accurate preoperative volumetric assessment of donor liver is essential for successful living donor liver transplant by ensuring adequate remnant and graft recipient weight ratio (GRWR). Methods: The study cohort consisted of 744 right lobe (RL), 65 left lobe (LL) and 33 left lateral sector (LLS) grafts from July 2010 to January 2014. A semi-automated interactive commercial software called AW Volume share 6 was used for volumetry. Bland Altman plot was used for assessing the agreement between estimated graft weight (EGW) and actual graft weight (AGW). Results: There was no statistically significant difference between EGW and AGW for RL graft weight (722±134 vs. 717±126 gm;P=0.06). Although Bland Altman graph showed that 95% limits of agreement was more in LL (?164 to +110) than RL (?156 to +147) and LLS grafts (?137 to +239), CT scan significantly overestimated LL graft weight (EGW =460±118 gm vs. AGW =433±102 gm;P=0.003) and underestimated LLS graft weight (EGW =203±48 gm vs. AGW =254±49 gm;P<0.001). Conclusions: CT volumetry overestimate LL graft and underestimate LLS graft weight. This should be factored in when selecting LL graft by taking higher GRWR.

关键词： Liver transplantation graft weight CT volumetry living donor

Endoscopic management of postoperative bile leaks

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 273-277页

作者： Naresh Agarwal Barjesh Chander Sharma Sanjay garg Rakesh kumar Shiv K Sarin Department of Gastroenterology GB Pant Hospital New DelhiIndia

BACKGROUND: Significant bile leak as an uncommon complication after biliary tract surgery may constitute a serious and difficult management problem. Surgical management of biliary fistulae is associated with high morbidity and mortality. Biliary endoscopic procedures have become the treatment of choice for management of biliary Gstulae. METHODS: Ninety patients presented with bile leaks after cholecystectomy ( open cholecystectomy in 45 patients, cholecystectomy with common bile duct exploration in 20 and laparoscopic cholecystectomy in 25). The presence of bile leaks was confirmed by ERCP and the appearance of bile in percutaneous drainage of abdominal collections. Of the 90 patients with postoperative bile leaks, 18 patients had complete transaction of the common bile duct by ERCP and were subjected to bilioenteric anastomosis. In the remaining patients after cholangiography and localization of the site of bile leaks. therapeutic procedures like sphinctero-tomy, biliary stenting and nasobiliary drainage ( NBD ) were performed. If residual stones were seen in the common bile duct, sphincterotomy was followed by stone extraction using dormia basket. Nasobiliary drain or stents of 7F size were placed according to the standard techniques. The NBD was removed when bile leak stopped and closure of the fistula confirmed cholangiographically. The stents were removed after an interval of 6-8 weeks. RESULTS: Bile leaks in 72 patients occurred in the cystic duct (38 patients), the common bile duct (30 ), and the right hepatic duct (4). Of the 72 patients with post-operative bile leak, 24 had associated retained common bile duct stones and 1 had ascaris in common bile duct. All the 72 patients were subjected to therapeutic procedures including sphincterotomy with stone extraction followed by biliary stenting (24 patients), removal of ascaris and biliary stenting (1), sphincterotomy with biliary stenting (18), sphincterotomy with NBD (12), biliary stenting alone (12), and NBD

关键词： biliary fistulae endoscopic retrograde cholangiopancreatography sphincterotomy biliary stent nasobiliary drain common bile duct stones

Cr(VI) Ions Removal from Aqueous Solutions Using Natural Adsorbents – FTIR Studies

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Journal of Environmental Protection 2011年第6期2卷 729-735页

作者： Biswajit Singha tarun kumar Naiya Ashim kumar Bhattacharya Sudip kumar Das 不详

The ability of eight natural adsorbents were investigated for adsorptive removal of Cr(VI) from aqueous solutions. Various physico-chemical parameters such as pH, initial metal ion concentration, adsorbent dose level and equi-librium contact time were optimized in batch adsorption technique. A detailed Fourier Transform Infrared Spectrophotometer (FTIR) study of adsorbents and Cr(VI) loaded adsorbents at the optimized condition was carried out to identify the different functional groups that were responsible for the adsorption. The important functional groups like hydroxyl, alkene, aromatic nitro, carboxilate anion, silicon oxide, sulphonic acid etc. were present in the natural adsorbent and were responsible for the chemical adsorption of Cr(VI) from aqueous solutions. The sorption energy calculated from Dubinin-Radushkevich isotherm indicated that the adsorption process were chemical in nature.

关键词： FTIR Chromium (VI) Rice Straw Hyacinth Roots Saw Dust

An Efficient Sound and Data Steganography Based Secure Authentication System

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Computers, Materials & Continua 2021年第4期67卷 723-751页

作者： Debajit Datta Lalit garg Kathiravan Srinivasan Atsushi Inoue G.Thippa Reddy M.Praveen kumar Reddy K.Ramesh Nidal Nasser School of Computer Science and Engineering Vellore Institute of Technology(VIT)Vellore632014India Faculty of Information and Communication Technology University of MaltaMsidaMSD2080Malta School of Information Technology and Engineering Vellore Institute of Technology(VIT)Vellore632014India Department of Information Systems&Business Analytics Eastern Washington UniversitySpokaneWA99202USA Department of Computer Science Karnataka State Akkamahadevi Women’s UniversityVijayapuraIndia College of Engineering Alfaisal UniversityRiyadh1153Saudi Arabia

The prodigious advancements in contemporary technologies have also brought in the situation of unprecedented ***,the pin-based security system is an inadequate mechanism for handling such a *** reason is that hackers use multiple strategies for evading security systems and thereby gaining access to private *** research proposes to deploy diverse approaches for authenticating and securing a connection amongst two devices/gadgets via sound,thereby disregarding the pins’manual ***,the results demonstrate that the proposed approaches outperform conventional pin-based authentication or QR authentication ***,a random signal is encrypted,and then it is transformed into a wave file,after which it gets transmitted in a short burst via the device’s ***,the other device/gadget captures these audio bursts through its microphone and decrypts the audio signal for getting the essential data for ***,this model requires two devices/gadgets with speakers and a microphone,and no extra hardware such as a camera,for reading the QR code is *** first module is tested with realtime data and generates high scores for the widely accepted accuracy metrics,including precision,Recall,F1 score,entropy,and mutual information(MI).Additionally,this work also proposes a module helps in a secured transmission of sensitive data by encrypting it over images and other *** steganographic module includes two-stage encryption with two different encryption algorithms to transmit data by embedding inside a *** encryption algorithms and their combinations are taken for this system to compare the resultant file *** these systems engender high accuracies and provide secure connectivity,leading to a sustainable communication ecosystem.

关键词： Cyber-attacks signals privacy security organization encryption decryption authentication effective communication steganography

Giant exophytic renal angiomyolipoma masquerading as a retroperitoneal liposarcoma: A case report and review of literature

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World Journal of Clinical Oncology 2018年第7期9卷 162-166页

作者： Gopal Sharma Ayush Jain Prerit Sharma Sonal Sharma Vinita Rathi Pankaj kumar garg Department of Surgery University College of Medical Sciences and Guru Teg Bahadur Hospital University of Delhi Department of Pathology University College of Medical Sciences and Guru Teg Bahadur Hospital University of Delhi Department of Radiology University College of Medical Sciences and Guru Teg Bahadur Hospital University of Delhi

A 42-years-old lady, presented with a large retroperitoneal mass which was preoperatively diagnosed as a retroperitoneal liposarcoma following an image guided core biopsy. She underwent a margin-negative resection of the retroperitoneal mass(multi visceral resection-enbloc excision of the retroperitoneal mass with a left nephrectomy and a segmental descending colectomy). The final histopathological examination of the resected specimen confirmed an exophytic renal angiomyolipoma(AML) which was extending into the retroperitoneum. AML is a rare benign tumor arising most commonly from the kidney. It can sometimes present as a diagnostic challenge as it mimics a retroperitoneal liposarcoma or a fat-containing renal cell carcinomas closely. We present this case to share our experience of managing a case of giant exophytic AML which resembled retroperitoneal liposarcoma closely and resulted into an aggressive surgery.

关键词： Angiomyolipoma Retroperitoneum Liposarcoma Diagnosis Biopsy

Depth profile exploration of enzyme activity and culturable microbial community from the oxygen-starved soil of Sundarban mangrove forest, India

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Open Journal of Ecology 2011年第3期1卷 65-72页

作者： Subhajit Das tarun kumar Sarkar Minati De Dipnarayan Ganguly Tusher Kanti Maiti Abhishek Mukherjee Tapan kumar Jana tarun kumar De 不详

Populations of culturable microbes and activities of dehydrogenase & ?-D glucosidase were found maximum in surface soil and decreased with increase in depth in Sundarban mangrove *** maximum (13.529 X 106 C.F.U g-1 dry weight of soil) and minimum (11.547 X 106 C.F.U g-1 dry weight of soil) total microbial popu- lations in surface soil were recorded during po- st-monsoon and monsoon respectively. At 60 cm depth, the lower (6.396 X 106 C.F.U g-1 dry weight of soil) and higher (8.003 X 106 C.F.U g-1 dry weight of soil) numbers of total microbial populations were observed during monsoon and post-monsoon respectively. A decreasing trend of total microbial load, enzyme activities and nutrient status with organic carbon were found with increase in depth throughout the year. Present study revealed the relationship among depth integrated variations of physico-chemical compo- nents (viz. soil temperature, pH, moisture, orga- nic-C, .nitrogen, and available-P) and microbial populations as well as activity of dehydrogenase and ?-D glucosidase enzymes.

关键词： Sundarban Mangrove Sediment Enzyme Activities Depth Microbial Populations

Hematological indices as predictors of mortality in dengue shock syndrome: A retrospective study

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Journal of Acute Disease 2022年第4期11卷 150-155页

作者： L.A.Gauri Ketan Bhatnagar Parvez Sameja Hardeva Ram Nehara Kuldeep Saini Ravi Dutt Bhupen Bhatnagar Mohit Singh Khokhar Vikas kumar Anjali garg S.P.Medical College BikanerRajasthanIndia AIIMS Jodhpur RajasthanIndia

Objective:To explore predictive hematological parameters on admission which are associated with mortality in NS1 positive dengue shock syndrome ***:Demographic characteristics,hematological parameters,and the outcome of NS1 positive dengue shock syndrome patients without any comorbidity and coexisting infections were collected from the Intensive Care Unit and the results were compared between the survivor and non-survivor ***:The mean age was(30.77±11.48)years and 56(56.6%)patients were *** of the total 99 patients,72(72.27%)patients were successfully discharged and 27(27.27%)patients eventually succumbed to *** most common hematological finding was thrombocytopenia(95.95%),followed by anemia(52.52%)and decreased mean platelet volume(37.37%).After controlling other variables,logistic regression analysis showed that absolute neutrophil count and mean platelet volume were associated with ***:Total leucocyte count,absolute neutrophil count,and total platelet count are significantly higher,and mean platelet volume is significantly lower in the non-survivor group as compared to the survivor *** neutrophil count and mean platelet volume are predictors associated with mortality.

关键词： Dengue shock syndrome NS1 Complete blood count Thrombocytopenia Leucocytosis White blood cell Absolute neutrophil count Mean platelet volume