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Effects of abiotic stress and hormones on the expressions of five13-CmLOXs and enzyme activity in oriental melon(Cucumis melo *** Makino)

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Journal of Integrative Agriculture 2016年第2期15卷 326-338页

作者： LIU Jie-ying ZHANG Chong SHAO Qi tang yu-fan CAO Song-xiao GUO Xiao-ou JIN Ya-zhong QI Hong-yan Key Laboratory of Protected Horticulture of Liaoning Province/Key Laboratory of Protected Horticulture Ministry of Education/College of HorticultureShenyang Agricultural University College of Agriculture Heilongjiang Bayi Agricultural University

Lipoxygenases（LOXs） are a group of non-heme,iron-containing enzymes and extensively involved in plant growth and development,ripening and senescence,stress responses,biosynthesis of regulatory molecules and defense *** our previous study,18 LOXs in melon genome were screened and identified,and five 13-LOX genes（CmLOX08,CmLOX10,CmLOX12,CmLOX13 and CmLOX18） were predicted to locate in *** analysis result showed that the five genes have high homology with jasmonic acid（JA） biosynthesis-related LOXs from other *** addition,promoter analysis revealed that motifs of the five genes participate in gene expression regulated by hormones and ***,we analyzed the expressions of the five genes and LOX activity in leaves of four-leaf stage seedlings of oriental melon cultivar yumeiren under abiotic stress：wounding,cold,high temperature and hydrogen peroxide（H_2O_2）,and signal molecule treatments：methyl jasmonate（MeJA）,abscisic acid（ABA） and salicylic acid（SA）.Real time qPCR revealed that wounding and H_2O_2 induced the expressions of all the five *** CmLOX08 was induced by cold while only CmLOX13 was suppressed by high *** induced the expressions of CmLOXIO and CmLOX12 while inhibited CmLOX13 and *** increased the 3 genes expressions except CmLOX08 and CmLOX13,whereas SA decreased the effect,apart from *** the abiotic stresses and signal molecules treatments increased the LOX activity in leaves of oriental *** summary,the results suggest that the five genes have diverse functions in abiotic stress and hormone responses,and might participate in defense *** data generated in this study will be helpful in subcellular localization and transgenic experiment to understand their precise roles in plant defense response.

关键词： oriental melons lipoxygenase abiotic stress signal molecules gene expression

Highly effective design of high GFA alloys with different metalbased and various components by machine learning

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Science China(Technological Sciences) 2024年第5期67卷 1431-1442页

作者： tang YiChuan HE Yifan fan ZhuoQun WANG ZhongQi tang ChengYing Guangxi Key Laboratory for Informational Materials School of Materials Science and EngineeringGuilin University of Electronic TechnologyGuilin541004China

The glass-forming ability(GFA)is of great significance for the development of novel functional metal-based metallic *** this study,seven popular machine learning(ML)algorithms were employed to design novel M-based(M=Fe,Co,Ni,Ti,Zr,and rare earth metal(RE))and X-component(X=2,3,4,5,6,and>6)alloys with excellent GFA.A GFA containing 6957 data points with structural analysis was *** engineering was used to analyze the importance and correlation of *** algorithms were utilized for GFA prediction,revealing that Xtreme Gradient Boosting Trees exhibited the strongest predictive capability,achieving a high accuracy of 94.0%,a true positive rate of 97.6%,and a root mean squared error of 0.3705 across the entire ***,the GFA of ternary to hexahydroxy alloys based on Fe,Co,Ni,Zr,Ti,and Y was predicted using all possible compositions generated through ***,a series of alloys with good GFA was successfully designed and *** present work suggests that the proposed ML method can be utilized to design novel multiple-M-based amorphous alloys with high GFA.

关键词： machine learning amorphous alloy feature engineering materials design glass forming ability

Improving photochemical indicators for attributing ozone sensitivities in source apportionment analysis

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Journal of Environmental Sciences 2024年第9期143卷 235-246页

作者： Xiaohui Du Wei tang Zhongzhi Zhang Yang yu Yang Li Ling Huang Greg Yarwood fan Meng Atmospheric Environment Institute Chinese Research Academy of Environmental SciencesBeijing 100012China College of Water Sciences Beijing Normal UniversityBeijing 100857China Asia Center for Air Pollution Research United Nations Environment Programme Asia and the Pacific OfficeNishi-kuNiigata-shi 9502144Japan School of Environmental and Chemical Engineering Shanghai UniversityShanghai 200444China Ramboll NovatoCalifornia 94945USA

Comprehensive Air Quality Model with extensions(CAMx)-Decoupled Direct Method(DDM)simulations of first-order ozone(O_(3))sensitivity to nitrogen oxides(NO_(x))and volatile organic compounds(VOCs)emissions were performed and combined with modelled P_(H_(2)O_(2))/P_(HNO_(3))ratios to obtain a range of thresholds for determining O_(3)-sensitivity regimes for different areas of *** the new threshold ranges for photochemical indicators,the method for determining O_(3)formation in the Ozone Source Apportionment Technology(OSAT)module within CAMx was improved by a dynamically varied threshold of P_(H_(2)O_(2))/P_(HNO_(3))*** O_(3)concentration contributions in the newly added transition regime were apportioned to NO_(x)and VOCs emissions in proportion to the relationship between the P_(H_(2)O_(2))/P_(HNO_(3))ratio and first-order O_(3)*** source contributions of O_(3)concentrations from different emission sectors from June to September 2019 were compared using the original and improved *** results showed that the O_(3)concentration contributions changed significantly in the NO_(x)-limited regime,with a maximum decrease of 21.89%,while the contributions increased by up to 7.57%in the VOC-limited regime,and were within 15μg/m^(3)in the transition *** modified OSAT module enabled a more sophisticated attribution of O_(3)to precursor emissions and may have far-reaching implications for informing O_(3)pollution control policy.

关键词： O_(3) modeling O_(3)-sensitivity regimes Ozone source apportionment technology Photochemical indicators

Cisplatin-based miRNA delivery strategy inspired by the circCPNE1/miR-330-3p pathway for oral squamous cell carcinoma

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Acta Pharmaceutica Sinica B 2024年第6期14卷 2748-2760页

作者： Hua-yang fan Ming-da Zhao Hong-jie Jiang Zhen-wei yu yu-jiang fan Xin-hua Liang Ya-ling tang Yong Sun State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Oral and Maxillofacial Surgery West China Hospital of StomatologySichuan UniversityChengdu 610041China National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610065China State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Oral Pathology West China Hospital of StomatologySichuan UniversityChengdu 610041China

Circular RNAs(circRNAs)are ideal biomarkers of oral squamous cell carcinoma(OSCC)because of their highly stable closed-loop structure,and they can act as microRNA(miRNA)sponges to regulate OSCC *** analyzing clinical samples,we identified circCPNE1,a dysregulated circRNA in OSCC,and its expression level was negatively correlated with the clinical stage of OSCC ***-of-function assays revealed the tumor-suppressive effect of circCPNE1,which was then identified as a miR-330-3p ***-330-3p was recognized as a tumor promoter in multiple studies,consistent with our finding that it could promote the proliferation,migration,and invasion of OSCC *** results indicated that selective inhibition of miR-330-3p could be an effective strategy to inhibit OSCC ***,we designed cationic polylysine-cisplatin prodrugs to deliver antagomiR-330-3p(a miRNA inhibitory analog)via electrostatic interactions to form PP@miR nanoparticles(NPs).Paratumoral administration results revealed that PP@miR NPs effectively inhibited subcutaneous tumor progression and achieved partial tumor elimination(2/5),which confirmed the critical role of miR-330-3p in OSCC *** findings provide a new perspective for the development of OSCC treatments.

关键词： Oral squamous cell carcinoma CircRNA MiRNA sponge Antagomir MiRNA delivery Cisplatin-based nanoparticles Drug delivery systems Tumor elimination

Amplifiable protein identification via residue-resolved barcoding and composition code counting

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National Science Review 2024年第7期11卷 205-217页

作者： Weiming Guo yuan Liu yu Han Huan tang Xinyuan fan Chu Wang Peng R.Chen Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular Engineering Peking University Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary Studies Peking University

Ultrasensitive protein identification is of paramount importance in basic research and clinical diagnostics but remains extremely challenging. A key bottleneck in preventing single-molecule protein sequencing is that, unlike the revolutionary nucleic acid sequencing methods that rely on the polymerase chain reaction(PCR) to amplify DNA and RNA molecules, protein molecules cannot be directly amplified. Decoding the proteins via amplification of certain fingerprints rather than the intact protein sequence thus represents an appealing alternative choice to address this formidable challenge. Herein, we report a proof-of-concept method that relies on residue-resolved DNA barcoding and composition code counting for amplifiable protein fingerprinting(AmproCode). In AmproCode, selective types of residues on peptides or proteins are chemically labeled with a DNA barcode, which can be amplified and quantified via quantitative PCR. The operation generates a relative ratio as the residue-resolved ‘composition code’ for each target protein that can be utilized as the fingerprint to determine its identity from the proteome database. We developed a database searching algorithm and applied it to assess the coverage of the whole proteome and secretome via computational simulations, proving the theoretical feasibility of AmproCode. We then designed the residue-specific DNA barcoding and amplification workflow, and identified different synthetic model peptides found in the secretome at as low as the fmol/L level for demonstration. These results build the foundation for an unprecedented amplifiable protein fingerprinting method. We believe that, in the future,AmproCode could ultimately realize single-molecule amplifiable identification of trace complex samples without further purification, and it may open a new avenue in the development of next-generation protein sequencing techniques.

关键词： protein identification amplifiable fingerprinting residue-specific chemistry DNA barcoding composition code counting

Characteristic Analysis of Power Oscillations Caused by Disharmony Among Voltage-source-controlled Units in Three-terminal Local Power Grid

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Journal of Modern Power Systems and Clean Energy 2024年第4期12卷 1295-1308页

作者： Qiyi yu Yi tang School of Electrical Engineering Southeast UniversityNanjing 210018China

Previous studies have demonstrated that disharmo-ny among voltage-source-controlled units(VSCUs)may occur on an alternating current(AC)transmission or distribution line under steady-state operating conditions(SSOCs)or quasi-static operating conditions(QSSOCs).As the studies on frequency dis-harmony have been expanded to multiple disharmonized vS-CUs in the local power grid,its adverse effects on AC lines and equivalent load(EL)at the bus without active voltage control ability(non-active bus)need to be investigated ***-ering the locality of disharmony and common topological con-nections among VSCUs,this paper adopts a Y-type three-termi-nal local power grid(LPG)as the research *** dishar-mony among the three VSCUs is ***,for the load at non-active bus,the formulas for single-phase instantaneous voltage,load current,load power,as well as average power un-der disharmony operating conditions(DOCs)are *** characteristic indicators of the above electrical quantities are defined,which can measure the amplification and reduction de-grees of the above electrical quantities before and after ***,for the line directly connected to VSCUs,the formulas for single-phase instantaneous line current and power and the average power under DOCs are *** character-istic indicators of power flow are defined,which can be used to quantify the peak amplification impact of oscillation before and after ***,the case study on the Y-type three-terminal LPG under the single-disharmony and the multi-dis-harmony switching scenarios indicates that the long-period pow-er oscillation caused by disharmony may occur in the load flow at the non-active bus and the line *** oscillation causes a serious decrease in load capability and a significant amplifica-tion of the peak of line power oscillation.

关键词： New power system power oscillation disharmony voltage-source-controlled unit(VSCU) characteristic analysis

Two-dimensional bimetallic selenium-containing metal-organic frameworks and their calcinated derivatives as electrocatalysts for overall water splitting

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Frontiers in Energy 2024年第3期18卷 378-389页

作者： Zhao-ting SHANG tang-ming LI Bing-qian HU Min LIU Wang-ting LU fan yu yun ZHENG Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education College of Optoelectronic Materials and TechnologyJianghan UniversityWuhan 430056China Institute of New Energy Materials and Engineering School of Materials Science and EngineeringFuzhou UniversityFuzhou 350108China Department of Chemical Engineering University of WaterlooWaterlooOntario N2L 3G1Canada

The use of two-dimensional(2D)layered metal-organic frameworks(MOFs)as self-sacrificial templates has been proven to be a successful method to create high-efficiency Selenium(Se)-containing electrocatalysts for overall water ***,two strategies are then utilized to introduce Se element into the Co–Fe MOF,one being the etching of as-prepared MOF by SeO_(2) solution,and the other,the replacing of SCN−with SeCN−as the construction *** electrochemical activity of the pristine 2D MOF and their calcinated derivatives for catalyzing the hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)is evaluated and further *** is found that the effect of introducing Se on improving electrochemical catalytic activity is significant for the HER ***,the calcinated derivative in the replacing method exhibits an overpotential of 235 mV for HER and 270 mV for OER at a current density of 10 mA/*** comparing the two methods of introducing Se element into MOF,similar electrocatalytic activity can be achieved on the their calcinated *** high electrochemical performance of 2D CoFe-MOF derivatives may be resulted from the unique 2D hierarchical porous structure and strong synergistic effect between different components in the material.

关键词： 2D MOF selenium(Se)-containing materials hydrogen evolution reaction(HER) oxygen evolution reaction(OER)

Inferring the spin distribution of binary black holes using deep learning

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Chinese Physics C 2024年第10期48卷 275-283页

作者： Li tang Xi-Long fan School of Math and Physics Mianyang Teachers'CollegeMianyang 621000China School of Physics and Technology Wuhan UniversityWuhan 430072 China

The spin characteristics of black holes offer valuable insights into the evolutionary pathways of their progenitor *** is crucial for understanding the broader population properties of black *** hierarchical Bayesian inference techniques employed to discern these properties often demand substantial time,and consensus regarding the spin distribution of binary black hole(BBH)systems remains *** this study,leveraging observations from GWTC-3,we adopted a machine learning approach to infer the spin distribution of black holes within BBH ***,we developed a deep neural network(DNN)and trained it using data generated from a Beta *** training strategy,involving the segregation of data into 10 bins,not only expedites model training but also enhances the versatility and adaptability of the DNN to accommodate the growing volume of gravitational wave *** Monte Carlo-bootstrap(MC-bootstrap)to generate observation-simulated samples,we derived spin distribution parameters:α=1.3_(-0.18)^(+0.25),β=1.70_(-0.29)^(+0.24)for the larger BH sample and α=1.37_(-0.20)^(+0.31),β=1.63_(-0.20)^(+0.30) for the smaller BH *** our constraints,the distributions of component spin magnitudes suggest the likelihood of both black holes in the BBH merger possessing non-zero spin.

关键词： gravitational wave black hole merges deep learning

Characteristics of the HIV/AIDS Epidemic among People Aged≥50 Years in China during 2018–2021

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Biomedical and Environmental Sciences 2024年第4期37卷 399-405页

作者： HOU yu Shan JIN Yi Chen CAI Chang tang Hou Lin QIN Qian Qian Lyu fan National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

Objective This study aimed to determine the current epidemiological status of PLWHA aged≥50 years in China from 2018 to *** also aimed to recommend targeted interventions for the prevention and treatment of HIV/AIDS in elderly *** Data on newly reported cases of PLWHA,aged≥50 years in China from 2018 to 2021,were collected using the *** tests and spatial analyses were also *** Between 2018 and 2021,237,724 HIV/AIDS cases were reported among patients aged≥50 years in *** main transmission route was heterosexual transmission(91.24%).Commercial heterosexual transmission(CHC)was the primary mode of transmission among males,while non-marital non-CHC([NMNCHC];60.59%)was the prevalent route in *** proportion of patients with CHC decreased over time(Z=67.716,P<0.01),while that of patients with NMNCHC increased(Z=153.05,P<0.01).The sex ratio varied among the different modes of infection,and it peaked at 17.65 for *** spatial analysis indicated spatial clustering,and the high-high clustering areas were mainly distributed in the southwestern and central-southern *** In China,PLWHA,aged≥50 years,were predominantly infected through heterosexual *** primary modes of infection were CHC and *** were variations in the sex ratio among different age groups,infected through various sexual ***/AIDS cases exhibited spatial *** on these results,the expansion of HIV testing,treatment,and integrated behavioral interventions in high-risk populations is recommended to enhance disease detection in key regions.

关键词： HIV/AIDS Aged≥50 years China