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Neural Regeneration Research 2024年第10期19卷 2240-2248页

作者： Meng Yuan yao tang Tianwen Huang Lining Ke En Huang Key Laboratory of Brain Aging and Neurodegenerative Diseases of Fujian Province Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Human Anatomy School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Scientific Research Center School of Basic Medical SciencesFujian Medical UniversityFuzhouFujian ProvinceChina Department of Neurology Fujian Medical University Union HospitalFuzhouFujian ProvinceChina Fujian Key Laboratory of Vascular Aging Fujian Medical UniversityFuzhouFujian ProvinceChina

In situ direct reprogramming technology can directly convert endogenous glial cells into functional neurons in vivo for central nervous system repair. Polypyrimidine tract-binding protein 1(PTB) knockdown has been shown to reprogram astrocytes to functional neurons in situ. In this study, we used AAV-PHP.e B-GFAP-sh PTB to knockdown PTB in a mouse model of ischemic stroke induced by endothelin-1, and investigated the effects of GFAP-sh PTB-mediated direct reprogramming to neurons. Our results showed that in the mouse model of ischemic stroke, PTB knockdown effectively reprogrammed GFAP-positive cells to neurons in ischemic foci, restored neural tissue structure, reduced inflammatory response, and improved behavioral function. These findings validate the effectiveness of in situ transdifferentiation of astrocytes, and suggest that the approach may be a promising strategy for stroke treatment.

关键词： astrocyte in situ direct reprogramming ischemic stroke miR-30 based shRNA neuron polypyrimidine tract-binding protein 1 transdifferentiation

Origin of the Yueguang gold deposit in Xinhua, Hunan Province, South China: insights from fl uid inclusion and hydrogen–oxygen stable isotope analysis

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Acta Geochimica 2024年第2期43卷 235-254页

作者： Hongxin Fan Qiang Wang Yulong Yang yao tang Hao Zou College of Earth Sciences Chengdu University of TechnologyChengdu 610059China College of Earth Sciences Jilin UniversityJilin 130061China

The Yueguang gold deposit is located in Fengjia,Xinhua County,Hunan Province,South China.It represents a recently discovered small-scale gold deposit situated in the southwestern region of the Jiangnan Orogenic Belt,west of the Baimashan granitic batholith.In order to discern the characteristics of the ore-formingﬂuids,the underlying mineralization processes,and establish a foundation for the origin of the Yueguang gold depositﬂuid inclusion micro-thermometry,as well as quartz hydrogen and oxygen isotope analysis,have been carried out on samples obtained from various stages of mineralization.The hydrothermal miner-alization stages within the Yueguang gold deposit can be categorized into three stages:(i)the barren,pre-ore quartz-pyrite stage(Stage Ⅰ),the quartz-pyrite-gold stage(Stage Ⅱ),and the post-ore quartz-carbonate stage(Stage Ⅲ),with the second stage being the main mineralization stage.Theﬂuid inclusions identiﬁed in samples from the main min-eralization stage can predominantly be described with the NaCl–H_(2)O and CO_(2)–NaCl–H_(2)O systems.These inclusions display homogenization temperatures ranging from 158.8 to 334.9℃,and theﬂuid salinity ranges from 0.3%to 4.0%(wt.%NaCl equiv.).Laser Raman spectroscopy analysis of individual inclusions further reveals the presence of gas-phases such as CO_(2),CH_(4),and N_(2).Isotopic analysis indicatesδ^(18)Oﬂuid values ranging from 3.95 to 6.7‰ and δDH_(2)O values ranging from-71.9 to-55.7‰.These results indi-cate that the ore-formingﬂuid of the Yueguang gold deposit belongs to metamorphic hydrothermalﬂuids of middle-low temperature and low salinity.In the process of ore formation,gold is transported in the form of Au(HS)2-complexes,with gold deposition being driven byﬂuid immiscibility.Therefore,the Yueguang gold deposit is categorized as an orogenic gold deposit dominated by metamorphic hydrother-malﬂuid.It may become a new target for gold exploration in the Baimashan region,central Hunan Province.

关键词： Hunan province Yueguang gold deposit Fluid inclusions Hydrogen–oxygen isotopes Laser Raman Fluid immiscibility Orogenic gold deposit

Development of the International Polar Years and their benefits for China

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Advances in Polar Science 2022年第2期33卷 192-198页

作者： tang yao Polar Research Institute of China Shanghai 200136China

International Polar Years,which have been held four times,have greatly promoted human understanding of the polar regions.The development of the International Polar Years has the following features:increasing interdisciplinary trend;importance of international organizations in initiating and participating in projects;and science diplomacy playing an important role in promoting cooperation and resolving differences.China was highly involved in the fourth International Polar Year in 2007-08,and the PANDA project which as a China-led international project marked a gradual shift in China’s polar activities.China could play a bigger role in the fifth International Polar Year,including the following:initiating a new International Polar Year;initiating more international projects;promoting international organizations;actively conducting science diplomacy;and publicizing its polar activities in different ways.

关键词： International Polar Year International Geophysical Year Antarctic Treaty China

Hypergravity experiments on multiphase media evolution

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Science China(Technological Sciences) 2022年第12期65卷 2791-2808页

作者： CHEN YunMin tang yao LING DaoSheng WANG YuBing Center for Hypergravity Experiment and Interdisciplinary Research Zhejiang UniversityHangzhou 310058China Key Laboratory of Soft Soils and Geoenvironmental Engineering(Ministry of Education) Zhejiang UniversityHangzhou 310058China

The gravitational field affects the evolution of multiphase media, such as rocks, soil, and alloy melts. Hypergravity increases the body force of matter, enhancing the driving force of the relative motion between substances with different densities and accelerating the evolution of multiphase media. Hypergravity experiments provide a new approach to exploring the motion of multiphase media and solving engineering problems. Hypergravity experiments have been conducted in different disciplines,such as materials science, geological science, and geotechnical engineering. However, the knowledge barriers between various research fields have caused the development of centrifuges/inflight devices and theoretical research on the mechanisms of matter in motion in hypergravity to lag behind the application of hypergravity experiments, limiting the progress in these experiments.This article systematically summarizes and proposes the fundamentals of hypergravity experiments, while the scientific challenge of the nonlinear hypergravity effect induced by high hypergravity on multiphase media evolution is clarified. Evaluation criteria are proposed for the noninertial frame effects of the centrifugal hypergravity field. The development of the high-centrifugal acceleration, large-capacity, and long-beam centrifuges are determined as the future research direction. Representative cases are used to demonstrate the effectiveness and great potential of the hypergravity experiments for the solidification of alloy melts and physical modeling. Challenges in the experimental methodology are also clarified. This paper reviews the fundamentals and applications of hypergravity experiments in various disciplines, pointing out the research direction of hypergravity experiments on multiphase media evolution.

关键词： hypergravity experiments multiphase media centrifuge solidification of alloy melts physical modeling

LSM6 promotes cell proliferation and migration regulated by HMGB1 in laryngeal squamous cell carcinoma

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Engineered Regeneration

Engineered Regeneration 2024年第2期5卷 247-254页

作者： Dengbin Ma Jiayi Li Hui Li yao tang Xia Gao Hong Chen Xiaoyun Qian Xiaohui Shen Department of Otolaryngology Head and Neck Surgery Research Institute of OtolaryngologyNanjing Drum Tower HospitalJiangsu Provincial Key Medical DisciplineAffiliated Hospital of medical SchoolNanjing UniversityNanjing210008China Department of Otorhinolaryngology Minda Hospital of Hubei Minzu UniversityEnshi445000China Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University 321 Zhongshan RoadNanjing210008China

Elevated levels of high mobility group protein B1(HMGB1)play a significant role in the pathogenesis of many diseases,but is particularly important for the formation of malignant tumors.Nonetheless,the function of HMGB1 and the underlying mechanism of laryngeal squamous cell carcinoma(LSCC)remain incompletely understood,causing uncertainty.Here we found immunohistochemistry from 97 LSCC tissues showed HMGB1 was upreg-ulated,which was associated with poor differentiation.HMGB1 knockdown could significantly inhibit wound closure and colony formation.The full-genome gene expression microarray was performed to investigate the mechanism.After knockdown of HMGB1 by siRNA,among the expressed differential genes,10 genes were ran-domly selected for validation.Then,shRNA lentivirus targeting these genes were constructed to explore their role in LSCC by cell proliferation assay.LSM6 downregulation was dramatically promoted by HMGB1 knockdown,resulting in higher expression in LSCC tissues.Furthermore,downregulation of LSM6 could significantly suppress cell proliferation,migration and colony formation.This study indicated that HMGB1 promoted LSCC cell malig-nant phenotypes through regulation of LSM6.We anticipate that HMGB1-LSM6 could be a putative therapeutic target for LSCC.

关键词： HMGB1 LSM6 Laryngeal squamous cell carcinoma Cell proliferation Migration

Aggregation-Induced Catalysis: Asymmetric Catalysis with Chiral Aggregates

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Research 2024年第1期2023卷 525-532页

作者： yao tang Yu Wang Qingkai Yuan Sai Zhang Jia-Yin Wang Shengzhou Jin Ting Xu Junyi Pan Kazimierz Surowiec Guigen Li Department of Chemistry and Biochemistry Texas Tech UniversityLubbockTX 79409-1061USA School of Chemistry and Chemical Engineering Nanjing UniversityNanjing 210093China School of Pharmacy Continuous Flow Engineering Laboratory of National Petroleum and Chemical IndustryChangzhou UniversityChangzhouJiangsu 213164China

So far,there have been 4 methods to control chirality including the use of chiral auxiliaries,reagents,solvents,and catalysts documented in literature and textbooks.Among them,asymmetric catalysts are normally divided into homogeneous and heterogeneous catalysis.In this report,we present a new type of asymmetric control—asymmetric catalysis via chiral aggregates that would not belong to the above categories.This new strategy is represented by catalytic asymmetric dihydroxylation reaction of olefins in which chiral ligands are aggregated by taking advantage of typical aggregation-induced emission systems containing tetrahydrofuran and H_(2)O cosolvents.It was proven that the chiral induction can be enhanced from er of 78:22 to 97:3 simply by changing the ratios of these 2 cosolvents.

关键词： chiral asymmetric Chiral

Cold plasma promotes Sertoli cell proliferation via AMPK–mTOR signaling pathway

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Journal of Integrative Agriculture 2022年第9期21卷 2700-2719页

作者： ZHANG Jiao-jiao LI Ya-qi SHI Mei WANG Yu-sha tang yao WANG Xian-zhong Chongqing Key Laboratory of Forage and Herbivore College of Veterinary MedicineSouthwest UniversityChongqing 400715P.R.China

This study investigated cold plasmas for multiple biological applications. Our previous work has found dielectric barrier discharge plasma improves chicken sperm quality. The number of Sertoli cells(SCs) decides spermatogenesis. However, whether cold plasma can regulate SC proliferation remains unclear. This study explored the effects of cold plasma on immature chicken SC proliferation and the regulation mechanism. Results showed that cold plasma exposure at 2.4 W for 30 s twice with an interval of 6 h produced(P<0.05) the maximum SC viability, cell growth, and cell cycle progression. SC proliferation-promoting effect of cold plasma treatment was regulated by increasing(P<0.05) the adenosine triphosphate production and the respiratory enzyme activity in the mitochondria. This process was potentially mediated by the adenosine monophosphate-activated protein kinase(AMPK)–mammalian target of rapamycin(m TOR) signaling pathway, which was regulated by the micro RNA(mi RNA) targeting regulation directly and by the intracellular reactive oxygen species homeostasis indirectly. The cold plasma treatment increased(P<0.01) the mi R-7450-5 p expression and led to a decreased(P<0.01) AMPKα1 level. On the other hand, mi R-100-5 p expression was reduced(P<0.05) and led to an increased(P<0.05) m TOR level in SCs. A single-stranded synthetic mi R-7450-5 p antagomir and a double-stranded synthetic mi R-100-5 p agomir reduced(P<0.05) the SC proliferation. However, this could be ameliorated(P<0.05) by the cold plasma treatment. Our findings suggest that appropriate cold plasma treatment provides a safe strategy to improve SC proliferation, which is beneficial to elevating male chicken reproductive capacity.

关键词： cold plasma Sertoli cell proliferation AMPK mTOR miRNAs

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人工智能应用如何影响企业创新宽度?

财经问题研究 2024年第2期 38-50页

作者：王钰唐要家浙江财经大学经济学院浙江杭州310018

人工智能是引领新一轮科技革命和产业变革的重要驱动力,如何充分释放人工智能促进创新和经济高质量发展的潜力是当前的重要课题。本文基于2011—2019年中国沪深A股制造业上市公司数据,采用多维固定效应模型实证研究了人工智能应用对企... 详细信息

人工智能是引领新一轮科技革命和产业变革的重要驱动力,如何充分释放人工智能促进创新和经济高质量发展的潜力是当前的重要课题。本文基于2011—2019年中国沪深A股制造业上市公司数据,采用多维固定效应模型实证研究了人工智能应用对企业创新宽度的影响及其作用机制。研究结果显示:人工智能应用能显著拓展企业创新宽度;人工智能应用对企业创新宽度的影响在中小企业、市场竞争力强、融资约束小和高科技行业的企业更明显;人工智能应用通过提高企业研发成功率、提升企业运营效率和增强企业竞争优势拓展企业创新宽度;人工智能应用滞后则会制约企业拓展创新宽度。充分释放人工智能应用促进高质量创新潜力的政策重点是:构建人工智能应用拓展企业创新宽度的创新生态,形成人工智能应用驱动创新的市场环境和政策环境,增强企业技术迭代能力以实现可持续创新。

关键词：人工智能应用企业创新宽度研发成功率运营效率竞争优势

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融入交叉注意力编码的皮肤病变分割网络

光学精密工程 2024年第4期32卷 609-621页

作者：李大湘杨福杰刘颖唐垚西安邮电大学通信与信息工程学院陕西西安710121

由于卷积操作的局限性,现有的皮肤病变图像分割网络无法对图像中的全局上下文信息建模,导致其无法有效捕获图像的目标结构信息,本文设计了一个融入交叉自注意力编码的U型混合网络,用于皮肤病变图像分割。首先,将设计的多头门控位置交叉... 详细信息

由于卷积操作的局限性,现有的皮肤病变图像分割网络无法对图像中的全局上下文信息建模,导致其无法有效捕获图像的目标结构信息,本文设计了一个融入交叉自注意力编码的U型混合网络,用于皮肤病变图像分割。首先,将设计的多头门控位置交叉自注意力编码器引入到U型网络的最后两个层级中,使其能够在图像中学习语义信息的长期依赖关系,弥补卷积操作全局建模能力的不足;其次,在跳跃连接部分引入一个新的位置通道注意力机制,用于编码融合特征的通道信息并保留位置信息,提高网络捕获目标结构的能力;最后,设计一个正则化Dice损失函数,使网络能够在假阳性和假阴性之间权衡,提高网络的分割结果。基于ISBI2017和ISIC2018数据集的对比实验结果表明,本文网络的Dice分别为91.48%和91.30%,IoU分别为84.42%和84.12%,分割精度在整体上优于其他网络,且具有较低的参数量和计算复杂度,即本文网络能够高效地分割皮肤病变图像的目标区域,可为皮肤疾病辅助诊断提供帮助。

关键词：医学图像分割皮肤病变交叉自注意力编码位置通道注意力

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基于比较视角的国土空间村庄规划编制探讨

山西建筑 2024年第3期50卷 44-46页

作者：唐尧浙江省国土空间规划研究院浙江杭州311100

现如今国土空间规划建设逐渐成熟,村庄规划编制形式丰富多样,国土空间体系大背景下系统整理村庄规划特点及核心要素十分必要,将为村庄规划提供有效理论支持。将城乡规划机制下村庄规划编制、实施、管理过程,置于国土空间体系下进行对比... 详细信息

现如今国土空间规划建设逐渐成熟,村庄规划编制形式丰富多样,国土空间体系大背景下系统整理村庄规划特点及核心要素十分必要,将为村庄规划提供有效理论支持。将城乡规划机制下村庄规划编制、实施、管理过程,置于国土空间体系下进行对比研究,认为国土空间规划系统呈现多元发展特点,有助于村庄规划多类型、多层级分布,实现全要素、全域化管理,关系乡村规划实用性、有效性,对于推动乡村特色建设、统筹城乡公共服务水平意义重大。

关键词：国土空间规划城乡规划村庄规划体系比较