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Synthesis of Site specifically Phosphate-caged siRNAs and Evaluation of their RNAi Activity and Stability

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Synthesis of Site specifically Phosphate-caged siRNAs and Ev...

中国化学会第29届学术年会

作者： xinjing tang State Key Laboratory of Natural and Biomimetic Drugs the School of Pharmaceutical SciencesPeking University

＜正＞A complete set of new photolabile nucleoside phosphoramidites were synthesized and then site-specifically incorporated in a siRNA strands for phosphate *** caging modification was made along siRNA strands and their photomodulation of gene silencing were *** key phosphate positions were then *** multiple caging modifications at these key positions led to significantly enhanced photomodulation of gene

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A Photochemical Avenue to Photoluminescence Nitrogen Rich Quantum Dots（N-dots）

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A Photochemical Avenue to Photoluminescence Nitrogen Rich Qu...

中国化学会第30届学术年会-第二十七分会：光化学

作者： Qingqing Jin xinjing tang School of Pharmaceutical Sciences Peking University

Quantum dots have been widely studied since 1990 s owing to their useful electronic and photonic *** our previous work, we discovered a nitrogen rich quantum dots（N-dots, up to 34% nitrogen and 10% more nitrogen than carbon element） by heating 2-azidoimidizole in methanol or aqueous solution and these N-dots showed excellent photoluminescence properties. Now, we, for the first time, demonstrated a photochemical avenue to synthesize N-dots from a small organic molecule, and further proposed a mechanism of N-dots *** N-dots could easily collected and the yield of collected N-dots was up to 92.7%, which was higher than that of thermodynamic preparation of *** N-dots have a small size（less than 10 nm） and contain a high percentage of the element nitrogen up to 35.70%, and show unique and distinct photoluminescence *** the N-dots were characterized by FTIR, HRTEM, and AFM,and their optical properties were evaluated in detail. These N-dots have also shown promising applications in two-photon fluorescence imaging.

关键词： bioimaging quantum dots N-Dots

Reduce off-target effect of RNA interference with circular siRNAs

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Reduce off-target effect of RNA interference with circular s...

中国化学会第30届学术年会-第二十八分会：化学生物学

作者： Yuan Wang xinjing tang State Key Laboratory of Natural and Biomimetic Drugs Peking University

Small interfering RNAs（siRNAs）play important roles in gene function study and drug ***,chemical modifications of siRNAs on backbone have been intensively investigated to increase the stability and specificity,*** of off-target effect of ***,we developed a novel type of circular siRNAs that are synthesized by cyclizing two ends of a single strand RNA（sense or antisense）using a T4 RNA *** newly synthesized circular siRNAs were more resistant to the degradation at the presence of SPVD or RNase *** addition,siRNA with circular antisense strand showed little effect on gene silencing,but siRNA with circular sense stand successfully knocked down gene expression of GFP or Luciferase in HEK293 *** results show that this new promising strategy could be used to overcome the off-target effect of strand selection on RNAi induced gene target.

关键词： siRNA circular RNA off-target effect gene expression

Novel Nitrogen-rich Quantum Dots and Their Applications

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Novel Nitrogen-rich Quantum Dots and Their Applications

第一届全国光功能材料青年学者研讨会2018

作者： QingQingJin JieFeng Xiuxian Chen xinjing tang State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking University

＜正＞New carbon based quantum dots have been discovered and investigated, such as nanodiamonds, graphene oxide（GOD）, C-dots. Here, we serendipitously synthesize novel nitrogen-rich quantum dots（N-dots） in methanol or aqueous solution with reaction temperature as low as 50 ℃ or only photoirrdiation with 365 nm UV light.

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不同品种辣椒制作的辣椒酱品质对比分析

食品与发酵工业 2023年第11期49卷 225-232页

作者：颜宇鸽段桂媛唐鑫静姚红杜木英西南大学食品科学学院重庆400715 中匈食品科学合作研究中心(西南大学) 重庆400715 川渝共建特色食品重庆重点实验室重庆400715

以遵义不同品种发酵辣椒酱为研究对象,分析辣椒酱在理化指标、辣椒碱、有机酸、游离氨基酸及挥发性成分的发酵特性,以期为专用型辣椒品种的有效利用和遵义地区辣椒加工产业链的完善提供依据。结果表明,不同品种发酵辣椒酱氨基酸态氮、... 详细信息

以遵义不同品种发酵辣椒酱为研究对象,分析辣椒酱在理化指标、辣椒碱、有机酸、游离氨基酸及挥发性成分的发酵特性,以期为专用型辣椒品种的有效利用和遵义地区辣椒加工产业链的完善提供依据。结果表明,不同品种发酵辣椒酱氨基酸态氮、色泽、类胡萝卜素含量和辣椒碱含量差异显著(P<0.05);5种辣椒酱pH、总酸、还原糖和亚硝酸盐含量无显著性差异;辣椒酱所含有机酸以苹果酸为主(40.2%~48.9%);游离氨基酸味觉活度值分析表明发酵辣椒酱的呈味氨基酸以鲜味氨基酸为主;5种辣椒酱共检测出挥发性物质152种,但不同辣椒品种间挥发性成分含量及种类差异较大,并确定了23种关键香气成分,其中水杨酸甲酯、香叶醇、D-柠檬烯及α-紫罗酮是共有香气成分。综合来看,遵辣7号和子弹头辣度较低,可用于低辣度产品开发;朝天椒及二荆条辣度较高,挥发性物质组成丰富,有机酸含量丰富,较适宜做泡椒、剁辣椒类产品的加工原料;牛角椒酱体细腻,色泽鲜艳,游离氨基酸含量[(4386.65±141.09)mg/kg]及挥发性成分含量[(20496.95±3984.51)μg/kg]最高,因此适宜传统发酵辣椒酱的开发及利用。

关键词：品种品质辣椒酱挥发性成分主成分分析

Caged siRNAs with 5’ terminal modification for target gene regulation

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Caged siRNAs with 5’ terminal modification for target gene ...

第十届全国化学生物学学术会议

作者： Yu,Lijia Yang,Jiali Chen,Changmai tang,xinjing State Key Laboratory of Natural and Biomimetic Drugs Peking University

Conditional regulation of siRNA functions will be highly useful, particularly through regulation of specific gene expression based on spatiotemporal resolution and ***, we will reported the synthesis of a series of caged siRNAs with terminal modification of different targeting moeities, such as cholesterol, folic acid and cRGD. Although single terminal labelling with these moieties did not guarrenteed their entrance to cells, their modifications on 5 terminal of antisense strand of siRNA could effeciently inhibit their corresponding siRNA gene silencing activities. Upon exposure to light irradiation, the terminal moieties were removed and siRNA activity was recovered for target gene *** these terminal modified caged siRNAs, we could achieve photomodulation of targeting gene expression in cells and also spatial regulation of GFP expression. For cRGD modified caged siRNA, we could also GFP gene expression in live mice tumor model with U87-GFP cells.

关键词： caged oligonucleotide siRNA cRGD Cholesterol gene silencing

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核酸光化学生物学

影像科学与光化学 2017年第4期35卷 477-493页

作者：张雪莉汤新景北京大学药学院天然药物及仿生药物国家重点实验室北京100191

光敏感基团作为光化学开关被广泛应用于各种生物过程的光调控中。特别是过去十几年内,核苷酸、寡聚核苷酸和DNA/RNA的光敏修饰策略得到了长足的发展,并在细胞信号传导和靶基因的功能调控等诸多生物学研究中发挥重要的作用。本文主要针... 详细信息

光敏感基团作为光化学开关被广泛应用于各种生物过程的光调控中。特别是过去十几年内,核苷酸、寡聚核苷酸和DNA/RNA的光敏修饰策略得到了长足的发展,并在细胞信号传导和靶基因的功能调控等诸多生物学研究中发挥重要的作用。本文主要针对常用的光敏感基团、光敏感核酸及其化学生物学研究进展进行简要综述,并对未来核酸光化学生物学的研究进行了展望。

关键词：光化学生物光敏感基团核苷酸核酸

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环状寡聚核苷酸的研究进展

中国科学：化学 2018年第11期48卷 1293-1306页

作者：经男男王静汤新景北京大学药学院天然药物及仿生药物国家重点实验室北京100191

环状结构的核酸因其首尾相连的特殊结构,可提高其对核酸外切酶的稳定性,并且其环状结构可使其具有特殊的功能.早期由于检测技术的限制,研究者对环状核酸的研究相对较少.近年来,随着科学技术的进步,研究者发现生物体内存在各种环状核酸,... 详细信息

环状结构的核酸因其首尾相连的特殊结构,可提高其对核酸外切酶的稳定性,并且其环状结构可使其具有特殊的功能.早期由于检测技术的限制,研究者对环状核酸的研究相对较少.近年来,随着科学技术的进步,研究者发现生物体内存在各种环状核酸,并具有独特的生理调节功能.对于较小的环状寡聚核苷酸(几十个碱基)在生命体中是否存在以及其可能的功能仍未知晓.本文综述了环状核酸(特别是较小环的寡聚核苷酸)主要的两类合成法(酶合成法和化学合成法),并比较了它们各自的优缺点.同时阐述了各种环状功能核酸的结构特性以及它们对特定生物活性和基因功能的特异性调控,为阐明环状核酸在生物体内的产生及其作用机制的化学生物学研究提供了新的研究工具.

关键词：环核酸反义核酸小干扰RNA 适配体基因沉默

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光敏感型嵌段聚合物胶束的研究进展

化工进展 2014年第9期33卷 2372-2379页

作者：余丽丽汤新景西安医学院药学院陕西西安710021 北京大学医学部药学院天然药物及仿生药物国家重点实验室北京100191

对聚合物结构中常见的光敏感性基团进行了介绍,并根据嵌段共聚物结构设计和光敏感基团的差异,将光敏感型嵌段聚合物胶束(BCPM)分为侧链变化型、主链断裂型、主链降解型和疏水交联型等几类,并对这几类BCPM的结构特征及其光刺激下结构的... 详细信息

对聚合物结构中常见的光敏感性基团进行了介绍,并根据嵌段共聚物结构设计和光敏感基团的差异,将光敏感型嵌段聚合物胶束(BCPM)分为侧链变化型、主链断裂型、主链降解型和疏水交联型等几类,并对这几类BCPM的结构特征及其光刺激下结构的变化进行了对比分析,重点阐明了各类BCPM作为药物载体材料时对药物的光控包载或释放行为。光敏感型BCPM具有良好的结构可控性和刺激响应能力,通过结构的进一步优化和研究的不断深入将有望发展成为一类新型的药物靶向输送系统。

关键词：光敏感嵌段共聚物胶束纳米粒子